RU2767896C1 - Antibacterial composition - Google Patents

Abstract

FIELD: antibacterial compositions.SUBSTANCE: invention relates to antibacterial compositions, can be used in chemical and cosmetic industry and is intended for inclusion in detergents both for sanitary purposes and personal hygiene products. Antibacterial composition contains benzalkonium chloride, honey-myrtles essential oil, D-limonene, aloe vera gel with the following content of components, wt.%: benzalkonium chloride 18–20, honey-myrtles essential oil 28–30, D-limonene 30–50, aloe vera gel 1–24.EFFECT: composition provides increased detergent power and skin care.1 cl, 4 tbl

Description

The invention relates to antibacterial compositions, can be used in the chemical, cosmetic industry, is intended for inclusion in the composition of detergents for both sanitary purposes and personal hygiene products.

The level of technology.

Known composition for personal hygiene according to patent No. 2435566, published 12/10/2011, containing at least 0.02 wt.% mixture of essential oil components containing:

(a) at least two acyclic components selected from citral, neral, garanial, geraniol and nerol, and

(b) at least two components containing ring structures selected from eucalyptol, eugenol and carvacrol,

wherein each component is present in an amount of at least 0.5 wt.% of the mixture.

The disadvantage of this composition is that the composition can only be used in personal care products.

Known antimicrobial composition according to application No. 99101105/14, published 27.11.2000, including: a) Antimicrobial agent selected from the group consisting of more than 30 vol.% alcohol and an effective amount of triclosan; and b) an effective amount of phenoxyethanol, benzalkonium chloride or benzethonium chloride; and phosphatidyldimonium chloride from cocoa butter.

The disadvantage of this composition is the use of triclosan as an antibacterial agent, since some bacteria can develop resistance to triclosan, rendering the antibacterial agent ineffective.

An active biological substance is known according to patent No. 2647460, published on March 15, 2018, which has antibacterial activity and antifungal activity, characterized by the presence of: eucalyptus extract, benzalkonium chloride and essential oils of eucalyptus, mint, fir, pine, lavender, tea tree, rosemary, coriander, rose and wormwood, as well as the presence of polysorbate 80 and polyvinylpyrrolidone in the following ratio of components, wt.%:

eucalyptus extract 10.3 benzalkonium chloride 10.3 essential eucalyptus oil 5.15 mint essential oil 5.15 fir essential oil 1.03 pine essential oil 1.03 lavender essential oil 1.03 tea tree essential oil 1.03 rosemary essential oil 1.03 coriander essential oil 1.03 rose essential oil 0.51 wormwood essential oil 0.51 polysorbate 80 51.60 polyvinylpyrrolidone 10.3

The disadvantage of this substance is a high risk of allergies and skin dermatitis, due to the wide range of essential oils in the composition.

Substance patent No. 2647460 is the closest analogue to the invention (prototype).

Disclosure of the essence of the invention.

The technical problem, the solution of which is provided by using the invention, is the creation of an antibacterial composition with increased washing power and the possibility of caring for the skin of the hands (moisturizing and healing the skin).

The technical result of the invention is to increase the washing power of the antibacterial composition and the possibility of caring for the skin of the hands.

To achieve a technical result, the antibacterial composition, including benzalkonium chloride, tea tree essential oil, according to the invention contains D-limonene, aloe vera gel, while the content of the components is, wt. %:

Benzalkonium chloride 18-20

Tea tree essential oil 28-30

D-limonene 30-50

Aloe Vera Gel 1-24.

Benzalkonium chloride has a bacteriostatic and bactericidal effect against gram-positive and gram-negative aerobic and anaerobic microorganisms: Staphylococus spp., Escherichia coli, Klebsiella spp., Proteus spp., Pseudomonas aeruginosa., Clostridium spp., Bacilus subtilis. It is more effective against Gram-positive bacteria. Benzalkonium chloride has a fungistatic and fungicidal effect on pathogenic fungi, in particular yeast-like fungi of the genus Candida. Reduces the resistance of bacteria and fungi to antibiotics. Effective against hospital microflora with polyresistance to chemotherapeutic agents, sexually transmitted infections (Treponema spp., Neisseria gonorrhoeae, Trichomonas spp., Chlamidia spp., Ureaplasma spp., as well as some viruses, including HIV, Herpes simplex) [one]. Benzalkonium chloride has a wide spectrum of antimicrobial activity against gram-positive and gram-negative microorganisms, including staphylococci, Pseudomonas aeruginosa and Escherichia coli. The mechanism of action is due to the ability to bind to ribosomes, which leads to irreversible inhibition of protein synthesis, as well as the ability to be fixed on the cytoplasmic membranes of bacteria, disrupting their permeability, while the cell loses potassium ions, amino acids, nucleotides [2].

Tea tree essential oil has a bacteriostatic and bactericidal effect against gram-positive and gram-negative aerobic and anaerobic microorganisms: E. coli, S. aureus, S. epidermidis, E. faecalis, P. aeruginosa, M. avium, H. influenzae, S. pyogenes , S. pneumonia, S. hominis, A. actinomycetemcomitans, L. monocytogenes, F. nucleatum, P. gingivalis, A. baumannii, A. viscosus Actinomyces spp, B. cereus, Bacteroides spp., Corynebacterium sp., E. faecium F. nucleatum, K. pneumonia, Lactobacillus spp., M. luteus, P. anaerobius, P. endodentalis, Prevotella spp, P. acnes, P. vulgaris, Veillonella spp[3].

D-limonene is a common terpene hydrocarbon derived from the essential oils of citrus plants. This natural, environmentally friendly and safe compound has a set of useful properties, such as high dissolving power in relation to hydrophobic contaminants, pleasant smell, antimicrobial, insecticidal and medicinal activity, volatility, chemical stability, high biodegradability, low toxicity [4].

Aloe vera gel is used as an emollient and moisturizer in cosmetics and personal care products. Aloe creams can be found in hundreds of skin products used for cosmetic purposes and wound healing [5].

The antibacterial effect is achieved due to:

- bacteriostatic and bactericidal action against gram-positive and gram-negative aerobic and anaerobic microorganisms of benzalkonium chloride;

- bacteriostatic and bactericidal action against gram-positive and gram-negative aerobic and anaerobic microorganisms of tea tree essential oil;

- antibacterial properties of D-limonene.

Detergency increases due to the high dissolving power of D-limonene in relation to hydrophobic contaminants.

The caring effect for the skin is achieved due to the moisturizing and healing properties of aloe vera gel.

Implementation of the invention.

The combination of the active components of tea tree essential oil, benzalkonium chloride, D-limonene and aloe vera gel in the active biological composition "SIDGUARD" provides an antibacterial effect with increased washing power and care for the skin of the hands.

To prepare the composition, the active ingredients are mixed: tea tree essential oil, benzalkonium chloride, D-limonene and aloe vera gel.

Formulation example: to prepare the composition, the active components are mixed, wt. %:

Benzalkonium chloride 18-20

Tea tree essential oil 28-30

D-limonene 30-50

Aloe Vera Gel 1-24.

The concentration of the composition, which ensures effectiveness in the means, is 0.2-1%.

The color of the finished antibacterial composition "SIDGUARD" can vary from light yellow to yellow, the shelf life is 36 months.

Table 1 shows three samples of the "SIDGUARD" composition.

Table 1

Composition Samples of the composition "SIDGUARD", wt. % one 2 3 Benzalkonium chloride eighteen twenty 19 Tea tree essential oil 28 29 thirty D-limonene thirty fifty 40 Aloe Vera Gel 24 one eleven

Based on the obtained sample No. 2, a 0.2% aqueous solution was prepared. Next, the antibacterial and antifungal activity of the SIDGUARD composition was determined. Microbial suspensions of microorganisms were prepared using the Densi-La-Meter device (according to the instructions for the device). Synchronization of cultures of strains was carried out at a low temperature. Microbial load was 10 ⁷ microbial cells/1 ml of medium. Worked with 20-24-hour cultures of strains of microorganisms.

The method of drug diffusion into agar was carried out by "wells". The activity of the composition was determined on two layers of nutrient medium immersed in Petri dishes. The bottom layer is a non-inoculated medium, the top layer is an agar nutrient medium, melted and cooled to 40°C, into which the corresponding standard of a daily culture of test strains was introduced. The volume of medium for the upper layer is from 14 to 16 ml. The cups were dried for 30-40 min at room temperature and placed in a thermostat for 20-24 hours.

table 2

A drug Diameters of growth retardation zones in mm in triplicate studies ATCC 25923 ATCC 25922 ATCC 4636 ATCC 27853 ATCC 6633 ATCC 653/885 SIDGUARD. 23,23,24 21,21,20 16,16,17 16,16,17 25,24,24 16,16,17

Based on the data in Table 2, it can be argued that SIDGUARD has antibacterial activity, bactericidal, bacteriostatic and antifungal effects.

To determine the washing ability, 0.2% of the SIDGUARD composition (sample No. 1) was added to an aqueous solution of surfactants. Washing ability was determined in accordance with GOST 22567.15-95. For comparison, we carried out a parallel determination of the washing ability in an aqueous solution of surfactants, without adding the SIDGUARD composition. The determination was carried out in triplicate.

Table 3

A drug Washing capacity Aqueous surfactant solution 85,84,87 Aqueous solution of surfactants with 0.2% composition "SIDGUARD", sample No. 1 98,97,100

According to the results of Table 3, it can be seen that the washing power of an aqueous solution of surfactant with the addition of 0.2% of the SIDGUARD composition of sample No. 1 is higher than that of an aqueous solution of surfactant without the addition of the composition (measurement error ±4).

A study was conducted to determine the effect on human skin of SIDGUARD compositions. On the wrists of 25 volunteers, 0.2% of sample #2 of the composition was applied. Moisture and oil measurements were taken after 2 hours using the Kelilong FCM-2 Skin Moisture Meter. The results obtained were compared with the moisture and oil content on the wrists of the volunteers prior to application with the "SIDGUARD" composition.

Table 4

Volunteers, no. Indicators after 2 hours of contact with the composition "SIDGUARD". Indicators before contact with the composition "SIDGUARD". Humidity, % Fat content, % Humidity, % Fat content, % one fifty 23 45 twenty 2 48 21 44 19 3 55 25 fifty 25 4 49 twenty 40 17 five 49 twenty 45 twenty 6 47 22 40 19 7 59 thirty 55 31 8 61 31 58 35 nine fifty twenty 45 17 10 49 21 45 19 eleven 60 33 56 31 12 57 25 51 22 13 49 23 45 19 fourteen 45 twenty 40 17 15 41 twenty 38 16 16 55 25 51 25 17 48 twenty 44 19 eighteen 55 23 fifty twenty 19 53 21 48 19 twenty fifty 28 46 25 21 55 thirty fifty 31 22 63 29 58 31 23 62 33 57 35 24 fifty twenty 45 17 25 53 22 48 19

From the results of Table 4, it can be seen that after applying the SIDGUARD composition to the skin of the volunteers, the moisture content increased. Most of the volunteers normalized oily skin.

Literature

1. Register of medicines of Russia [Electronic resource] / Encyclopedia of medicines and pharmacy products. - Moscow, 2012.

2. Compendium. Medicines [Electronic resource] / Specialized medical online publication for doctors, pharmacists, pharmacists, students of medical and pharmaceutical universities. - Kyiv, 2011.

3. Orchard A, van Vuuren S. Commercial Essential Oils as Potential Antimicrobials to Treat Skin Diseases. – Evidence Based Complement Alternat Med.2017;2017:4517971. doi: 10.1155/2017/4517971. Epub 2017 May 4.

4. Merkulov D.A., Raw materials and packaging magazine: for perfumery, cosmetics and household chemicals; Unique properties and uses of natural D-Limonene from citrus essential oils.-Moscow, 2020.

5. Glushchenko S.N., Shmygareva A.A., Sankov A.N.; Journal BULLETIN OF VORONEZH STATE UNIVERSITY. SERIES: CHEMISTRY. BIOLOGY. PHARMACY; MORPHOLOGICAL-ANATOMICAL STUDY OF THE ALOE-VERA LEAF.-Voronezh, 2018.

Claims (2)

An antibacterial composition comprising benzalkonium chloride, tea tree essential oil, characterized in that it contains D-limonene, aloe vera gel, while the content of the components is, wt. %: Benzalkonium chloride 18-20 Tea tree essential oil 28-30 D-limonene 30-50 Aloe Vera Gel 1-24.