RU2618889C1 - Mouthwash agent having antiinflammatory, antimicrobial, wound-healing effect - Google Patents

Abstract

FIELD: pharmacology.

SUBSTANCE: invention relates to mouthwash agent having antiinflammatory, antimicrobial, wound-healing effect, which represents leaf hydrolate Gynura procumbens obtained by steam or water distillation of fresh or dried leaves Gynura procumbens.

EFFECT: agent is effective for mouthwash, prevention and treatment of various diseases of the oral cavity due to the pronounced antiinflammatory, antimicrobial, wound-healing effect.

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Description

The invention relates to the pharmaceutical industry, in particular to a medicinal product based on plant materials, leaves of Gynura Procumbens, for rinsing the oral cavity, which has anti-inflammatory, antimicrobial, wound healing effect.

Inflammation of the oral mucosa develops as a result of trauma, a bacterial or viral infection, often accompanies some infectious diseases, hypovitaminosis, and allergic conditions. Stomatitis is characterized by hyperemia and soreness of the oral mucosa. With aphthous stomatitis, painful round-shaped elements with a whitish-gray bloom and a zone of hyperemia appear on it. Possible increase in body temperature, increase and soreness of the regional lymph nodes. Hemorrhagic, ulcerative and ulcerative necrotic forms of stomatitis are severe. Often, the disease acquires a chronic recurrent course.

With stomatitis, local (analgesic, antiseptic), general strengthening and desensitizing therapy is carried out. For pain relief, novocaine (2-5% solution), primocaine (1-2% solution), trimecaine (2-5% solution), lidocaine, or xicaine (1-2% solution) are used to lubricate the foci of inflammation. A suspension of anestezin in sunflower oil is also recommended.

Iodinol (1% solution), boric acid (2% solution), ethacridine sodium (0.05% solution), furatsilin (0.02% solution) are used as antiseptic solutions for rinsing, irrigation and washing. For fungal infections of the mucous membrane, decamethoxin (0.02% solution), a suspension of sodium tetraborate in glycerin are used.

Some aerosol preparations based on plant materials have antimicrobial and anti-inflammatory properties, for example, Ingalipt (contains streptocide, norsulfazole, thymol, eucalyptus oil, peppermint oil, alcohol, glycerin), Oxycort (contains hydrocortisone and oxytetracycline), Proposol ( contains propolis, glycerin, ethyl alcohol).

If there are no signs of secondary infection, you can use agents that have anti-inflammatory and astringent properties (oak bark, St. John's wort grass, sage leaves).

Reparative processes in the oral cavity are accelerated by sea buckthorn and rosehip oil.

For rinsing the oral cavity with stomatitis, various collections of plants that contain chamomile flowers, sage leaves, mint, fennel fruits are useful; or sage leaves, marshmallow roots, elderberry flowers. Fees are also being prepared on the basis of bark, oak, St. John's wort, marjoram, marigold, linden flowers [1].

Gingivitis is an inflammation or infection of the gums and alveolar bones that support the teeth. It is generally believed that gingivitis is caused by bacteria in the mouth (especially bacteria caused by plaque formation) and toxins formed as bacteria by-products. Periodontitis is commonly believed to occur where unremoved plaque hardens, turning into tartar (dense dental deposits), which affects periodontal ligaments. Periodontitis is a gradually worsened condition of the disease compared with gingivitis. As plaque and tartar continue to gradually increase in thickness, the gums begin to move away from the teeth and pockets form between the teeth and gums, which can ultimately lead to destruction of the bone and periodontal ligament. Such reactions lead to the destruction of the supporting structure, to constant infection and, potentially, to subsequent tooth loss.

Thus, there is a need to create a harmless, highly effective antibacterial, anti-inflammatory, wound healing product for rinsing the oral cavity, to prevent or mitigate diseases in the oral cavity.

According to the WHO, inflammatory periodontal diseases currently affect 70-90% of the world's adult population. This disease is the main cause of tooth loss. In this regard, the treatment of inflammatory periodontal diseases is still one of the urgent problems of modern dentistry. The high frequency of spread of gingivitis and periodontitis, various forms of their manifestation, the occurrence of foci of chronic infection in the oral cavity, tooth loss and, as a result, decreased performance indicate the relevance of increasing the effectiveness of treatment of periodontal pathology.

Known means for rinsing the oral cavity, which is a collection of rhizomes of calamus, rhizomes of the potentilla erect, chamomile inflorescences. A decoction is prepared: 1-2 teaspoons of the collection are insisted for 3 hours in a glass of boiling water, boiled for 10 minutes and used to rinse the mouth and throat with angina, laryngitis and stomatitis [2].

Known means for rinsing the oral cavity, which is a collection of flowers of buckwheat, flowers of poppy, flowers of mother and stepmother, grass of medunitsa, flowers of mallow forest - all 10 g. Prepare an infusion: 4 tbsp. collection spoons insist for 2-3 hours in 0.6 l of boiling water and drink with honey to taste 1 / 2-2 / 3 cups of warm infusion 3-4 times a day before meals with laryngitis, catarrh of the throat and diploma voice [3].

Known means for rinsing the oral cavity, which is a collection of: dioecious nettle leaves - 20 g, upright rhizome rhizomes - 15 g, chopped dill fruits - 10 g, medicinal sage leaves - 20 g. A decoction is prepared: 1-2 teaspoons of the collection are insisted 3 hours in a glass of boiling water, boil for 10 minutes and use to rinse your mouth and throat with angina, laryngitis and stomatitis [4].

Known means for rinsing the oral cavity, which use eucalyptus leaves and / or oil from them. Rinse is prepared - 1 tablespoon of a decoction of leaves in 1 glass of water or 10-20 drops of eucalyptus oil in a glass of water. Used as a bactericidal agent for rinsing and inhalation in inflammatory diseases in the oral cavity [5].

A disadvantage of the known drugs is the individual intolerance to certain components of the drug that is observed in some patients, insufficient anti-inflammatory, wound healing, antimicrobial activity.

The authors of the present invention developed a composition based on plant materials - leaves of Gynura Procumbens.

Gynura Procumbens from the family of asters was born many centuries ago on the African continent, then the plant successfully migrated to Asia, where it was appreciated by the healers of China and Southeast Asia. It was used simply as food and at the same time as a cure for disease. In Southeast Asia, Ginura is part of a balanced diet and healthy lifestyle. Phytochemical studies confirm that the extract from Ginura leaves is an excellent natural source of many potent biologically active components, including flavonoids, carotenoids, alkaloids and essential oils. An active effect on metabolic processes puts Ginuru in a number of other medicinal plants, which are actively used as primary protection for cancer patients before chemotherapy and radiotherapy. The results of clinical studies confirm the therapeutic effect of ginura in diabetes, kidney disease, gastroenterological and cardiovascular diseases. The plant has pronounced antioxidant properties, inhibits the growth of cancer cells in the body, has an anti-inflammatory effect and helps to increase reproductive function.

From the document [6] it is known that Gynura Procumbens is widely used in medicine for the treatment of inflammation, rheumatism, and viral diseases. Gynura Procumbens alcohol extract was tested for anti-inflammatory activity, which was fractionated in water and ethyl acetate. Gynura Procumbens aqueous extract has been shown to have no anti-inflammatory effect, while organic extraction exhibits pronounced anti-inflammatory activity.

The paper [7] studied the antioxidant activity of Gynura Procumbens. Gynura Procumbens methanol extract was fractionated in chloroform, ethyl acetate, butanol, water. Among all the extracts, the ethyl acetate fraction showed more pronounced antioxidant activity.

From the document [8], the antiproliferative effect of alcohol extraction of Gynura Procumbens leaves is known.

The paper [9] investigated the chemopreventive effect of Gynura Procumbens extract. The results showed that Gynura Procumbens has antiproliferative, antioxidant properties.

The document [10] discloses a dragee (candy) for oral hygiene, for the prevention of diseases of the oral cavity, throat. The candy contains 1-30 mass% of ginura grass in the form of micropowder. This tool is not effective enough for the prevention of diseases of the oral cavity, has a low antibacterial, anti-inflammatory effect.

The document [11] discloses a composition for treating the oral cavity, in the form of an oral spray, containing in its composition an alcohol extract of ginura. The composition has a low antibacterial activity, anti-inflammatory activity.

A document [12] discloses a mouthwash that contains juice from the roots of Gynura. Gynura Procumbens leaves are characterized by a unique composition of biologically active substances, which differs from the composition of the roots of the plant. The tool from the above document has a low antibacterial, anti-inflammatory activity.

The document [13] discloses a composition for treating an oral ulcer containing from 12 to 16 parts of two-flowered Ginura. The document [14] discloses a composition for treating oral cancer containing a two-flowered Ginur. The above documents do not disclose the use of Gynura Procumbens leaves as an oral rinse.

The authors of the present invention unexpectedly found that the hydrolyte from the leaves of Gynura Procumbens has a high activity - anti-inflammatory, antimicrobial, wound healing and can be used to rinse the mouth for the prevention and treatment of various diseases of the oral cavity.

Hydrolates (hydrosols) - water obtained by steam or water distillation of water through plant material. When water vapor passes through plant material, they are saturated with valuable components contained in plants: essential oils, acids, bioflavonoids, vitamins and other valuable substances. Steam extracts more valuable substances from the plant than water, therefore, the content of extracted substances in the hydrolyte is much higher than in ordinary broth, while various “ballast” substances — salts, dyes, etc., do not get into the hydrolyte, so the effect of the hydrolyte is much more effective and harmless than the effects of a decoction. Hydrolates contain about 0.2-0.5% (sometimes more) of water-soluble components of plants distributed in water in ionized form.

Hydrolates differ in properties from essential oils, as they consist of an aqueous phase and a water-soluble plant extract. Essential oils are contained in ordinary hydrolyte in a concentration optimal for use in a pure, undiluted form [15].

Hydrolates retain all the beneficial qualities of essential oils, but unlike the latter, they act very gently without causing burns or allergic reactions, they are safe for use on delicate areas of the body, as well as on skin prone to inflammation. The main advantage of using hydrolytes in comparison with concentrated essential oils is their more gentle effect on the skin, mucous membranes, etc.

The document [16] discloses the use of tea tree leaf hydrolyte, which has antibacterial, anti-inflammatory activity, for treating oral diseases. This tool is selected as a prototype. The disadvantage of the proposed hydrolyte is a low anti-inflammatory, antimicrobial, wound healing effect.

The objective of the proposed invention was to develop a tool based on the leaves of Gynura Procumbens, which is effective for rinsing the oral cavity, and can be used for the prevention and treatment of diseases of the oral cavity due to the high anti-inflammatory, antimicrobial, wound healing effect.

The technical result is that the proposed tool is a hydrolyte of leaves of Gynura Procumbens, which has a high anti-inflammatory, antimicrobial, wound healing effect, is effective for rinsing the mouth, preventing and treating diseases of the oral cavity, such as stomatitis, gingivitis, periodontitis, etc. .d.

The technical result is achieved by the fact that the proposed tool for the treatment of skin diseases, has anti-inflammatory, antimicrobial, wound healing, action, is a hydrolyte from the leaves of Ginura Prokumbens (Gynura procumbens), obtained using the method of steam or water distillation of fresh leaves in the ratio of fresh leaves and water 1: 3-6, or dry leaves with a ratio of dry leaves and water 1: 30-60.

During clinical studies, it was found that the optimal ratio of feedstock and water for steam or water distillation is: for fresh leaves 1: 3-6, for dry leaves 1: 30-60, respectively.

The inventors have unexpectedly found that the hydrolyte obtained from the leaves of Gynura Procumbens is characterized by a high content of biologically active substances that are not found in other products from the leaves of Gynura Procumbens, such as aqueous, alcoholic, ethyl acetate extract, as well as in known plant hydrolytes.

The presence of amino acids with antibacterial, immunomodulating and regenerating properties, in combination with the most important trace elements in organic form (associated with amino acids), provide high penetration into the cells and high antimicrobial properties. This fact is one of the distinguishing features of the hydrolyte from the leaves of Gynura Procumbens from other hydrolytes.

The hydrolyte from Gynura Procumbens leaves also contains essential oils, as well as Chlorophyll A and B, which significantly increase its effectiveness.

Getting hydrolyte from the leaves of Gynura Procumbens.

To obtain leaf hydrolyte, Gynura Procumbens used the standard method for producing plant hydrolytes using a distillation unit that operates in steam and water distillation modes.

Fresh or dry leaves of Gynura Procumbens were taken to obtain the hydrolyte. The ratio of plant materials and water for fresh leaves is 1.3-6, for dry leaves 1: 30-60, respectively.

To obtain the hydrolyte by steam distillation, fresh or dry leaves of Gynura Procumbens are placed on the grill of a distillation unit located at least 5 cm above the upper water level.

To obtain hydrolyte by water distillation, fresh or dry leaves of Gynura Procumbens are placed directly in a vessel with water.

The vessel with water is heated to a high temperature and the steam begins to break the glands of the plant, while releasing the essence of the plant in the form of steam. The steam is collected in a pipe passing through the cooling units, after which it again takes on a liquid form and is discharged into a sump.

The resulting hydrolyte and the fraction of essential oils are separated.

The composition of the resulting hydrolyte from the leaves of Gynura Procumbens was studied using a spectral-dynamic complex (certified in the EU as KME, Medicial Expert Complex).

According to the results of the study, the following groups of biologically active substances were isolated in the hydrolyte from the leaves of Gynura Procumbens.

Amino acids: serine, lysine, threonine, alanine, glutamine, citrulin, cystine, threonine, cysteine; organic acids: succinic acid, isovaleric acid; macro and micro elements: palladium, lithium, platinum, cobalt, bromine, gold, iron, zinc.

Gynura Procumbens leaf hydrolyte also contains chlorophyll A, B, and essential oils.

The obtained hydrolytes were used to rinse and irrigate the gums and oral mucosa in a volume of 20 ml for gingivitis, periodontal disease, multiple caries, stomatitis, etc.

The study of the pharmacological activity of hydrolyte from fresh or dry leaves of Gynura Procumbens and its comparison with the hydrolyte of the prototype and an aqueous extract of Gynura Procumbens leaves

Example 1

The minimum inhibitory concentration according to the dilution micromethod in the broth.

For anaerobes, 190 μl of a sterile pre-reconstituted Wilkins Chalgren Anaerobic Broth (WC) with 0.075% cysteine was added to the first well of a test series in a 96-well plate, followed by 100 μl of each of the four additional test wells. 10 μl of Gynura Procumbens leaf hydrolyte was added to the first well of the series. The contents of the first well were thoroughly mixed and serially diluted (1: 1) in the remaining four wells of the series; 100 μl were discarded from the last well of the series. Suspensions of Porphyromanas gingivalis ATCC 33277 or Prevotella intermedia ATCC 25611 diluted to about 1 × 10 8 CFU (colony forming units) per ml were added to the test wells in an amount of 100 μl. Appropriate controls for growth were included. The plates were incubated at 37 ° C. anaerobically until turbidity was observed in the growth control well. BMD (minimum inhibitory concentration) was recorded as the lowest concentration inhibiting the growth of the test organism, which was observed with the naked eye. Streptococcus mutans ATCC 25175 was similarly tested with tryptic soy broth (TSB) as the test medium and the plate was incubated at 37 ° C in an atmosphere with 5% CO2. Staphylococcus aureus ATCC 6538 was tested using tryptic soy broth (TSB) as a test medium and the plate was incubated at 37 ° C. Aspergillus niger ATCC 16404 was tested with tryptic soy broth (TSB) as a test medium and incubated at room temperature (25 ° C).

The results were recorded as the most probable value for three experiments for each bacterium. P. intermedia and P. gingivalis are gram-negative bacteria involved in the pathological process of periodontitis. Streptococcus mutans is involved in the formation of caries. The proposed tool, which is a hydrolyte from the leaves of the ginura prokumbens, is able to inhibit the growth of these 3 pathogenic bacteria at the lowest concentrations of the tested drug. Also, the proposed tool showed a high anti-inflammatory activity.

The prototype tool inhibited the growth of 3 pathogenic bacteria at higher concentrations of the test drug, namely at concentrations 1.5 times the concentration of hydrolyte from the leaves of Guinura Prokumbens.

When using an aqueous extract of Ginura Prokumbens, there is no inhibition of the growth of 3 pathogenic bacteria in the same concentration that was used to study the hydrolyte of leaves of Ginura Prokumbens.

The effect of Ginura Prokumbens leaf hydrolyte on the viability of P. gingivalis cells was investigated using a study of the reduction of tetrazolium sodium-3 '- {1 - [(phenylamino) carbonyl] -3,4-tetrazolium} bis (4-methoxy-6-nitro) benzenesulfonic hydrate acids (XTT). In summary, XTT was dissolved in PBS at a concentration of 1 mg / ml, and menadione in acetone was prepared at a concentration of 1 mM. XTT / menadione reagent contained 12.5 parts XTT / 1 part menadione. A 48-hour P. gingivalis biofilm was prepared as described above and treated with Ginura Prokumbens leaf hydrolyte (0 to 250 μg / ml) for 2 hours (anaerobiosis, 37 ° C.) before adding 25 μl of XTT / menadione. After 1 hour at 37 ° C., absorbance was read at 490 nm (A490) using a microplate reader. The hydrolyte of this invention was able to inhibit the growth of P. gingivalis, as well as inhibit the formation of P. gingivalis. Then P. gingivalis was unable to adhere (release). When the hydrolyte from the leaves of Ginura Prokumbens was applied after the biofilm was formed, there was no desorption of the biofilm, but the viability of the biofilm was reduced, which means that the biofilm was not removed, but that the bacteria that formed the biofilm died.

Thus, a hydrolyte from freshly picked or dried leaves of Gynura Procumbens exhibits a pronounced antimicrobial activity.

Example 2

Gynura Procumbens leaf hydrolyte caused a significant inhibition of macrophage production of interleukin 1β, interleukin 8 and tumor necrosis factor a obtained from U937 cells, a cell line of human monoblastic leukemia, stimulated with LPS from Aggregatibacter actinomycetemcomitans at a concentration of 10 μg / ml in 5 μg / ml in 5 μg / ml and 5 μg against interleukin 6. These results are confirmed in two independent experiments.

The proposed hydrolyte caused a significant suppression of the production of interleukin 6, interleukin 8 and tumor necrosis factor-α (TNF-α) by macrophages stimulated by LPS P. gingivalis in a concentration in the range of 5-10 μg / ml. These results are confirmed in two independent experiments. At the same time, it was shown that the tea tree hydrolyte and Gynura Procumbens aqueous extract showed low anti-inflammatory activity.

Example 3

We examined and treated with hydrolyte from the leaves of Ginura Prokumbens (20 men and 19 women aged 25 to 55 years) with chronic generalized periodontitis of mild and moderate severity, rinsing 10-15 ml 2-3 times a day for 2- 3 weeks. The control was a group of patients of the same age with a similar diagnosis using tea tree leaf hydrolyte. Evaluation of treatment results was carried out according to the clinical research methods in dynamics: examination, determination of the depth of periodontal pockets, Fedorov-Volodkina hygiene index (IG), PMA index, Schiller-Pisarev test, bleeding index (IR), determination of the functional resistance of capillaries according to V.I. . Kulazhenko (FSK), the state of periodontal vessels microcirculation was determined by laser Doppler flowmetry using the LAKK-01 apparatus.

Prior to treatment, all patients underwent oral sanitation; recommendations were given on special oral hygiene. After preliminary antiseptic treatment, over- and subgingival deposits were removed with a piezoelectric scaler (P-5 BOOSTER SUPRASSON).

Research results.

Treatment of patients with hydrolyzed leaves of Ginura Prokumbens by rinsing the mouth 2-3 times a day allowed for a short time (5-7 days in the main group versus 13-18 days in the control) to obtain a persistent therapeutic effect. Clinical improvement was confirmed by the positive dynamics of changes in clinical indices.

During the observation period (6 months) in patients of the main group there was no exacerbation of the disease. In the control group, 40% of the subjects had recurrence of the disease within 1.5-2 months after the course of treatment.

The use of Ginura Prokumbens aqueous extract for the treatment of mild chronic generalized periodontitis has also been investigated. Clinical studies have shown that a positive effect was observed only on day 20-21.

Example 4

Patient V., 35 years old, complained of pain while chewing food, an unpleasant burning sensation and dry mouth, bad breath, and bleeding gums. According to the patient, these symptoms appeared 2 weeks after treatment by an orthopedic dentist about restoring the integrity of the upper dentition with a partial denture.

Objectively: the mucous membrane of the oral cavity of the upper jaw is hyperemic, edematous, in the area of 12-17 teeth - pinpoint hemorrhages, bleeding erosion and aphthae are determined. In the area of 13, 14, 15, 16 teeth - pathological gingival pockets up to 5 mm and more than 5 mm deep, with the release of serous-purulent discharge, in the area of 45, 46 and 47 teeth - inflammation with ulceration of the gums, the gingival contour is hypertrophied by more than 1/3 of the tooth crown, there is a recession of the gums. IG (OHI-S) was 2.5 points (poor oral hygiene). To make an accurate diagnosis of the patient, a mucous test was performed, the result after 12 hours - hyperemia with swelling, after 48 hours - hyperemia with small bubbles.

Diagnosis: hypersensitivity of the oral mucosa, stomatitis of the dentition, periodontitis.

Treatment. The patient has been replaced with a denture, desensitizing therapy, heavy drinking, rinsing with the developed product 4 times a day, 5-7 minutes, delaying the solution in the mouth after rinsing for 5-10 minutes, were prescribed.

Next visit: on the second day after the application of the developed product, the patient notes a sharp decrease in pain and burning of the mucous membrane. After 4 days, with an objective study, the aphthae decreased in size; when touched, they do not bleed. Purulent-serous discharge when probing the gingival pockets is not determined. The hygiene index after 6 days after the start of treatment was 0.95 points (good condition), the Grossman index, Fedi - 0.5 points (normal gum, elastic consistency, no signs of exudation, gingival pockets no more than 3 mm), gingival-periodontal the index is 0.8 points for the gums (good condition) and 3.5 points for the periodontium (the probe does not sink deeper than 3 mm). The results obtained indicate a complete relief of the inflammatory process, the wound healing effect of the agent, a decrease in the formation of plaque, and a decrease in the virulence of oral microorganisms.

Example 5

Patient V., 30 years old, complained of bad breath, pain when chewing and food getting between the teeth of the lower jaw on the right.

зуба), пародонтальный индекс Рамфьорда - 2,29 балла (воспаление средней тяжести - охватывает всю десну вокруг зуба).Objectively: 33, 34-37 under a bridge of plastic metal prosthesis. The mucous membrane in the area of the prosthesis is hyperemic, the gingival papillae 33-37 are swollen, with ulceration along the vestibular surface, the gum in the region of 37 is swollen, hyperemic, bleeds when touched by a probe. Periodontal pockets in the region of 33, 34, the mesial surface of the 37 tooth - 5 mm, with exudative discharge. IG - 3.8 points (unsatisfactory condition), the Rumfjord hygiene index - 3.7 points (tooth plaque is present on all proximal, buccal, lingual surfaces, but does not cover more

tooth), the periodontal Rumfjord index - 2.29 points (moderate inflammation - covers the entire gum around the tooth).

Diagnosis: periodontitis in the area of the bridge in 33, 34 and 38 teeth.

Treatment. Frequent rinses of the oral cavity with the developed product at least 4 times a day are prescribed. After 2 days, the patient noted a decrease in pain and swelling of the gums, a decrease in bleeding and the size of foci of gum ulceration in the region of 33, 34, 35 teeth. After 7 days, the patient makes no complaints, there is no discharge from the gingival pockets, IG - 1.03 points (good hygiene), gingival-periodontal index - 0.83 points (good condition of the gums and periodontium). The patient was under observation for another 1 month, no complaints were noted. The data obtained indicate a rapid remission of the disease immediately after the start of using the developed product, a decrease in plaque.

Example 6. (using the prototype).

Patient S., 38 years old, complained of pain when chewing and biting on a removable lower jaw prosthesis.

Objectively: The mucous membrane of the oral cavity in the lower jaw is hyperemic, with 3 foci of ulceration (aphthae), round in shape, 1 × 1.5 cm in size and 0.5 × 1.5 cm in the region of the transitional fold on the left. Hygienic condition of the oral cavity is unsatisfactory (IG - more than 3.6 points), in the area of chewing and front teeth of the upper jaw - periodontal pockets, up to 5 mm deep, with purulent-exudative discharge.

Diagnosis. Dentistry stomatitis, periodontitis.

Treatment. Replacement of a removable denture. Assigned to rinse the mouth with a tool containing hydrolyte from tea tree leaves (prototype). After 2 days, there was a slight improvement in the state of the oral cavity, according to the patient, the pain during chewing remained. After 1 week, the state of oral hygiene was 3.4 points (unsatisfactory), exudative discharge from the gingival pockets of the teeth of the lower jaw, bad breath.

After 2 weeks, the IG 1.9 points - (satisfactory), gingive-periodontal index - 2.44 points (moderate), bleeding from the gingival pockets during sounding is noted. After 4 weeks, the patient's condition improved, IG - 0.93 points (good), Grossman index, Fedi - 1.97 points (moderate), but complete recovery did not occur, which required a significant correction in the course of treatment.

Thus, the obtained data indicate a positive effect of the developed agent as an anti-inflammatory, antimicrobial, wound healing agent for rinsing the oral cavity for various diseases of the oral cavity, exceeding the similar effect in the prototype and other extracts.

The claimed composition of the drug was used in 65 patients, all received a positive result on average 80.5% faster than other similar hygiene products.

Thus, as shown by the results of a clinical study, the developed tool differs from analogues in its high anti-inflammatory, antimicrobial, wound healing, hemostatic, decongestant properties, which allow it to be effectively used to rinse the oral cavity in cases of pathology of periodontal tissues and oral mucosa.

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Claims (1)

An agent with anti-inflammatory, antimicrobial, wound-healing action for rinsing the oral cavity, which is a hydrolyte of Gynura procumbens leaves, obtained by steam or water distillation of fresh or dry leaves of Gynura procumbens, in the ratio of fresh leaves to water 1: 3-6 or in the ratio of dry leaves and water 1: 30-60.