RU2527349C1 - Method for measuring amino acid absorption rate in digestive tract - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: fasting blood test and blood test following taking amino acid mixture are conducted. First, fasting circulating blood volume, hematocrit and total calculated blood amino acid nitrogen content are determined in the morning. That is followed by oral administration of amino acid solution 100.0 ml transported according to one of absorption options with the known concentration of the solution and the amino acid nitrogen content therein The circulating blood volume, the hematocrit and the total calculated amino acid nitrogen content are determined again 30, 40, 50, 60, and 70 minutes after the administration of the amino acid sequence. The blood samples are measured for an amino acid absorption ratio by formula: $$K=\frac{N_n\times CB{V}_n\times \left(100-H{t}_n/100\right)-N_1\times CB{V}_1\times \left(100-H{t}_1/100\right)}{N},$$

wherein K is the amino acid absorption ratio, N is the nitrogen content in the amino acid mixture, N₁ is a blood amino acid nitrogen value before taking amino acids, N_n is a blood amino acid nitrogen value after taking amino acids, CBV₁ is a circulatory blood volume before taking amino acids, CBV_n is a circulatory blood volume after taking amino acids, Ht₁ is a hematocrit value before taking amino acids, Ht_n is a hematocrit value after taking amino acids, n is a time following the oral administration of amino acids. Taking into account the ratio K and the time following the oral administration of amino acids, a diagram reflecting the amino acid absorption rate in the digestive tract is plotted.

EFFECT: method enables measuring the total amino acid absorption rate in the digestive tract taking into account the transport system characteristics.

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Description

The invention relates to medicine, to the field of gastroenterology and directly to the study of the absorption of amino acids in the gastrointestinal tract.

It is known that the maximum absorption in the digestive tract of up to 95% of amino acids occurs within 30-50 minutes after a meal. The different rate of penetration of amino acids through cell membranes indicates the presence of transport systems that ensure the transfer of amino acids, both through the outer plasma membrane and through intracellular membranes. Currently, five specific transport systems are known, each of which functions to transfer a certain group of amino acids with similar structure:

- neutral, with a short side chain (alanine, series, threonine);

- neutral, with a long or branched side chain (valine, leucine, isoleucine);

- with cationic radicals (lysine, arginine);

- with anionic radicals (glutamic and aspartic acids);

- amino acids (proline, hydroxyproline) (see Biochemistry: Textbook. / Ed. by E.S. Severin. - 4th ed. rev. - M: GEOTAR-Media, 2005. S.461-469).

In this case, the absorption rate of a specific amino acid, but not similar in the structure of amino acids, was estimated, which made it possible to identify the features of absorption of each amino acid in a particular transport system. We have not come across publications that mention methods for determining the rate of absorption of amino acids, the assimilation of which is via a common transport system for them. As a rule, the content of a specific amino acid in the blood was determined before and after a meal.

However, the determination of a specific amino acid in the blood before and after ingestion of food allows us to judge only about its absorption in the digestive tract, but not the total amino acids for a particular transport path.

The task of the invention is to assess the rate of total absorption of amino acids from the digestive tract, taking into account the characteristics of the transport system.

This is achieved by the fact that a method is used to study the rate of absorption of amino acids from the digestive tract, namely, that blood is tested in the morning on an empty stomach and after taking an amino acid mixture, characterized in that in the morning on an empty stomach they determine the volume of circulating blood, hematocrit and the total estimated nitrogen content of amino acids in the blood, then 100.0 ml of a solution of amino acids is transported orally, transported according to one of the absorption options with a known concentration of the solution and its content in m amino nitrogen, then after 30, 40, 50, 60 and 70 minutes to re-determine the blood volume, hematocrit and total amino acid nitrogen content calculated, after which the amino acids calculated blood absorption coefficient of the samples according to the formula:

, $$\mathrm{TO}=\frac{N_{\underset{\_}{n}}\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_{\underset{\_}{n}}\times \left(\mathrm{one\; hundred}-H{t}_{\underset{\_}{n}}/\mathrm{one\; hundred}\right)-N_{\underset{\_}{\mathrm{one}}}\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_{\underset{\_}{\mathrm{one}}}\times \left(\mathrm{one\; hundred}-H{t}_{\underset{\_}{\mathrm{one}}}/\mathrm{one\; hundred}\right)}{N}$$

,

where K is the absorption coefficient of amino acids,

N is the nitrogen content in the amino acid mixture,

N ₁ - indicators of nitrogen amino acids before taking amino acids,

N _n - indicators of nitrogen amino acids after taking amino acids,

BCC ₁ - the volume of circulating blood before taking amino acids,

BCC _n is the volume of circulating blood after taking amino acids,

Ht ₁ - hematocrit before taking amino acids,

Ht _n - hematocrit after taking amino acids,

n is the time after oral administration of amino acids, and, taking into account the results of the coefficient K coefficient and the time elapsed after administration of the amino acid solution, a graph is constructed that reflects the rate of absorption of amino acids in the digestive tract.

The proposed method for studying the rate of absorption of amino acids in the digestive tract allows you to evaluate the absorption of amino acids in the digestive tract according to the studied transport system, taking into account the structure of amino acids.

The method is as follows: in the morning on an empty stomach they determine the volume of circulating blood, collect and determine the hematocrit in the blood and calculate the total nitrogen content of amino acids in it, after which 100.0 ml of the amino acid mixture is orally administered and after 30, 40, 50, 60 and 70 minutes re-determine the volume of circulating blood, hematocrit and calculate the total nitrogen content of amino acids, and then calculate in a blood sample with maximum residual nitrogen the absorption coefficient of amino acids by the formula:

, $$\mathrm{TO}=\frac{N_{\underset{\_}{2}}\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_{\underset{\_}{2}}\times \left(\mathrm{one\; hundred}-H{t}_{\underset{\_}{2}}/\mathrm{one\; hundred}\right)-N_{\underset{\_}{\mathrm{one}}}\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_{\underset{\_}{\mathrm{one}}}\times \left(\mathrm{one\; hundred}-H{t}_{\underset{\_}{\mathrm{one}}}/\mathrm{one\; hundred}\right)}{N}$$

,

Where K is the absorption coefficient of amino acids,

N is the nitrogen content in the amino acid mixture,

N ₁ - indicators of nitrogen amino acids before taking amino acids,

N _n - indicators of nitrogen amino acids after taking amino acids,

BCC ₁ - the volume of circulating blood before taking amino acids,

BCC _n is the volume of circulating blood after taking amino acids,

Ht ₁ - hematocrit before taking amino acids,

Ht _n - hematocrit after taking amino acids,

n is the time after oral administration of amino acids,

and, taking into account the results of the coefficient K coefficient and the time elapsed after administration of the amino acid solution, a graph is plotted reflecting the rate of absorption of amino acids in the digestive tract.

This technique with a positive result was applied in 12 men and 5 women aged 18 to 34 years for two consecutive days. After determining the absorption of amino acids on the first day, non-invasive orthograde orthograde intestinal lavage was performed with Macrogol 4000 (Fortran). It was found that after cleaning the digestive tract, the absorption of amino acids in the digestive tract increased.

Example: For a volunteer S., 52 years old, when determining the rate of absorption of amino acids, the volume of circulating blood, hematocrit and nitrogen metabolism in the blood were determined on an empty stomach in the morning and 30, 40, 50, 60 and 70 minutes after taking 100.0 ml 40% a preparation containing leucine, isoleucine and valine (a BCAA sports nutrition preparation), and in accordance with the methodology of the method for studying the absorption rate of amino acids, the values of the coefficient K were determined in the study time. After 30 minutes, K = 0.56; after 40 minutes, K = 0.69; after 50 minutes, K = 0.74; after 60 minutes, K = 0.79; after 70 minutes, K = 0.81. The results obtained reflected the features of the rate of absorption of amino acids in the digestive tract and indicated a decrease in the rate of absorption of amino acids, since 90-95% of amino acids are absorbed within 30-50 minutes, and, accordingly, the values of the coefficient of absorption of amino acids should be K≥0.9. After cleaning the intestines with the drug Macrogol-4000 (Fortran), a second study of the absorption of amino acids in the digestive tract was carried out according to the proposed method. It was found that after 50 minutes the maximum value of the coefficient K = 0.91 was noted, which indicated the normalization of the rate of absorption of amino acids in the digestive tract, that is, slagging of the intestine can lead to a decrease in the rate of absorption of amino acids in the digestive tract.

Thus, the method for studying the rate of absorption of amino acids in the digestive tract allows us to estimate the rate of total absorption of amino acids from the digestive tract, taking into account the characteristics of the transport system.

Claims (1)

A method for studying the rate of absorption of amino acids from the digestive tract, which consists in the fact that they perform blood tests in the morning on an empty stomach and after taking an amino acid mixture, characterized in that in the morning on an empty stomach they determine the volume of circulating blood, hematocrit and the total calculated nitrogen content of amino acids in the blood, then orally administered 100.0 ml of a solution of amino acids transported according to one of the absorption options with a known concentration of the solution and the nitrogen content of amino acids in it, after that Erez 30, 40, 50, 60 and 70 minutes to re-determine the blood volume, hematocrit and total amino acid nitrogen content calculated, after which blood samples are calculated in the coefficient of absorption of amino acids formula:
$$\mathrm{TO}=\frac{N_n\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_n\times \left(\mathrm{one\; hundred}-H{t}_n/\mathrm{one\; hundred}\right)-N_{\mathrm{one}}\times \mathrm{ABOUT}\mathrm{Ts}{\mathrm{TO}}_{\mathrm{one}}\times \left(\mathrm{one\; hundred}-H{t}_{\mathrm{one}}/\mathrm{one\; hundred}\right)}{N}$$

,
where K is the absorption coefficient of amino acids,
N is the nitrogen content in the amino acid mixture,
N ₁ - indicators of nitrogen amino acids before taking amino acids,
N _n - indicators of nitrogen amino acids after taking amino acids,
BCC ₁ - the volume of circulating blood before taking amino acids,
BCC _n is the volume of circulating blood after taking amino acids,
Ht ₁ - hematocrit before taking amino acids,
Ht _n - hematocrit after taking amino acids,
n is the time after oral administration of amino acids,
and, taking into account the results of the coefficient K coefficient and the time elapsed after administration of the amino acid solution, a graph is plotted that reflects the rate of absorption of amino acids in the digestive tract.