RU2500418C1 - Hepatoprotective herbal tea - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to a hepatoprotective herbal tea. The hepatoprotective herbal tea that contains black currant leaves, red raspberry leaves, great willow herb, meadowsweet herb in certain proportions.

EFFECT: herbal tea provides the effective hepatoprotective action.

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Description

The invention relates to medicine, in particular to pharmacy, and is a collection of medicinal plant materials with hepatoprotective action.

Known collection with hepatoprotective and anti-inflammatory activities (RF Patent No. 2419447 C2, publ. 05/27/2011), containing inflorescences of calendula officinalis; peppermint leaves; Scutellaria baicalensis roots; rhizomes of a frangipani; black leaves of frankincense; shoots of shrubbery and used for the treatment and prevention of alcoholic hepatitis.

A known method for the complex treatment of chronic viral hepatitis B and C (RF Patent No. 2341275 C1, publ. 12/20/2008), including infusion from the collection No. 1 of medicinal plants containing oak bark, clover grass, licorice roots, bird of highlander, burdock roots, coltsfoot leaves, motherwort grass, cinnamon grass, echinacea grass, and in the remission phase, infusion from collection No. 2 containing rhizomes with elecampane roots, calendula flowers, nettle leaves, plantain leaves, rose hips, common wormwood grass, bean leaves Echinacea herb.

A known method of obtaining funds with hepatoprotective and choleretic action (RF Patent No. 2292897 C1, publ. 02/10/2007). The method consists in the extraction of crushed raw materials and separating the extract, the raw materials used are the collection of medicinal plant materials: hill hodgepodge grass, celandine grass, tansy flowers and sequential three times extraction with 70% ethanol and purified water at a ratio of raw materials: extractant 1: 1 method step countercurrent extraction.

Known hepatoprotectors of plant origin are legal (silymarin, silibinin), carlsil, obtained from the fruits of milk thistle, which are closest in pharmacological activity to the proposed hepatoprotective collection from medicinal plant materials and are recommended as comparison drugs in the study of hepatoprotective drugs [Khabriev R.U. . Guidelines for the experimental (preclinical) study of new pharmacological substances., Moscow. 2005, S.683-691].

The objective of the invention is the expansion of the arsenal of hepatoprotective agents of plant origin.

The problem is solved by the collection of hepatoprotective action, including leaves of black currant, leaves of raspberry vulgaris, fireweed narrow-leaved grass and grass meadowsweet with their equal weight ratio.

The use of this collection as a hepatoprotective agent was made possible thanks to the experimental identification of its new properties, namely, the therapeutic and prophylactic effect in toxic liver damage. Water infusion from the proposed collection has a pronounced hepatoprotective activity, not inferior to the reference drug - carlsyl. The ability of this collection to prevent and eliminate the effects of toxic liver damage was identified during experimental studies.

Concrete example

Dried, crushed and sifted through a sieve with a diameter of 0.5 mm leaves of raspberry ordinary 10.0; blackcurrant leaves 10.0; narrow-leaved fireweed grass 10.0; the grass of the meadowsweet 10.0 is mixed, placed in a container with a capacity of 1000 ml, 400 ml of distilled water are added, taking into account the coefficient of water absorption (K _VP = 3.0). Heated in a boiling water bath for 15 minutes, cooled at room temperature for 45 minutes. After cooling, the extract is filtered through a gauze filter.

At the first stage of the studies, a comparative study of the therapeutic and prophylactic effect of the developed collection and the reference drug - carzil in CCl ₄ -induced toxic hepatitis was carried out. The study was performed on 60 white outbred male rats weighing 220-240 g, randomly distributed in 4 groups:

1st group - intact rats (n = 22);

2nd group - control group (n = 22);

3rd group - experimental, claimed collection (n = 28);

4th group — experimental, reference preparation — carlsil (n = 28).

Animals were kept under standard vivarium conditions on a standard feeding diet. The first experimental group for three weeks received water extract from the claimed collection. During the first two weeks of the experiment, the experimental animals received infusion under conditions of free soldering, on average, 30 ml of infusion per day per animal. The second experimental group received Karsil (Sopharma, Bulgaria) at a dose of 200 mg / kg intragastrically as a suspension, as a reference preparation with hepatoprotective activity. Animals from the control and intact groups received ad librum water.

On the 15th day of the experiment, toxic hepatitis was induced in the experimental and control groups of animals by intraperitoneal administration of CCl ₄ in the form of a 25% solution in olive oil, once at a dose of 0.2 ml / kg (in terms of CCl ₄ ). After which the animals continued to receive hepatoprotectors for another 7 days. Thus, in the experiment, the therapeutic and prophylactic use of hepatoprotectors was simulated.

The hepatoprotective effect of the test collection was evaluated using a thiopental test. Thiopental was administered intraperitoneally at a dose of 50 mg / kg and the duration of thiopental anesthesia was recorded according to the “lateral position” indicator. The test allows you to evaluate the rate of thiopental metabolism carried out by cytochrome P-450-dependent monooxygenase system of hepatocytes.

The results of the experiment were processed using t-student criterion and are presented in table 1.

Table 1 The effect of aqueous extracts from the collection of medicinal plant materials and the prototype on the duration of thiopental anesthesia in animals (min) No. p \ p Group of animals The number of animals in the group □ x ± m one. Intact 12 59.8 ± 6.27 2. The control 12 168.1 ± 9.68 * 3. Experience (claimed fee) eighteen 40.7 ± 4.83 *; ** four. Experience (Karsil) eighteen 46.9 ± 3.84 ** Notes: * - significantly different from intact (P <0.05); ** - significantly different from control (P <0.05).

CCl ₄ hepatitis is characterized by the development of collication necrosis, protein and fatty degeneration of hepatocytes, localized mainly in the central zone of the hepatic lobule, where the activity of cytochrome P-450 monooxygenases is maximal and production of damaging metabolites of hepatotoxin predominates. From the data obtained in the experiment it is seen that the duration of thiopental anesthesia in animals exposed to hepatotoxin (control), the duration of thiopental anesthesia was almost 3 times higher than that of intact. The therapeutic and prophylactic use of the studied collection completely prevented these CCl ₄ -induced changes. It should be noted that a comparison of the hepatoprotective effect for this indicator (the duration of thiopental anesthesia) did not reveal a significant difference from the same indicator of the comparison drug - carzil. This indicates less liver damage in the experimental group (the claimed collection) and the preserved function of the cytochrome P-450-dependent monooxygenase system of hepatocytes.

Some biochemical and enzymatic blood parameters were also used as informative criteria for assessing the safety of liver tissue and the hepatoprotective effectiveness of the proposed collection.

In experimental animals that were not exposed to thiopental, on the 21st day from the start of the experiment, after decapitation, blood was taken to determine cholesterol, bilirubin (total, direct, indirect), ALT, ACT. Blood was centrifuged for 10 min at 3000 rpm on a Scanspeed mini centrifuge (USA). Plasma was taken and blood biochemical parameters were evaluated using kits from Intermedica (USA), according to the manufacturer's instructions on a biochemistry analyzer from Biochem (USA).

table 2 Changes in the main biochemical parameters in rat blood serum when applying the claimed collection on a model of experimental hepatitis Key biochemical indicators Intact animals Control CCl ₄ Experience: CCl ₄ + claimed fee Experience: CCl ₄ + Carsil ALT, ME 35.0 ± 3.6 220.5 ± 24.8 ^* 63.1 ± 6.0 ^{*; **} 54.3 ± 6.19 ^** ACT, ME 136.8 ± 14.7 1326.8 ± 184.6 ^* 570.3 ± 61.4 ^{*; **} 487.4 ± 57.8 ^** Cholesterol mmol / L 1.41 ± 0.05 2.68 ± 0.10 ^* 1.89 ± 0.05 ^{*; **} 1.75 ± 0.05 ^** Bilirubin total μmol / L 10.88 ± 0.34 134.68 ± 8.00 ^* 90.10 ± 6.60 ^{*; **} 87.6 ± 7.05 ^** Direct bilirubin micromol / l 3.18 ± 0.17 64.05 ± 2.05 ^* 38.10 ± 3.08 ^{*; **} 37.21 ± 3.11 ^** Indirect bilirubin μmol / L 7.70 ± 0.5 78.63 ± 7.07 ^* 52.00 ± 3.93 ^{*; **} 50.39 ± 3.92 ^** Note: * - significantly different from intact (P <0.05); ** - significant difference with control (P <0.05).

As can be seen from the results shown in table 2, in the control group of CCl ₄ hepatitis there was a significant increase in the level of liver enzymes, cholesterol, total, direct and indirect bilirubin, caused by exposure to hepatotoxin. In the group receiving the claimed collection against hepatitis, these shifts were significantly less pronounced. It should be noted that the data obtained in the “CCl ₄ + declared collection” group are only slightly inferior to the results obtained in the “CCl ₄ + carlsil” animal group.

Thus, the data obtained in the experiment indicate the presence of hepatoprotective properties in the collection consisting of common raspberry leaves, black currant leaves, narrow-leaved fireweed grass, and common meadowsweet grass.

Claims (1)

The collection of hepatoprotective action, characterized in that it contains leaves of black currant, leaves of raspberry vulgaris, fireweed narrow-leaved grass and grass meadowsweet with their equal weight ratio.