RU2568588C2 - Application of water with negative redox below zero potential as base for gels for biologically active additives in form of gel - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to application of water with stable negative redox potential from -201 to - 225 mV as solvent for manufacturing biologically active additives in form of gels, containing glutathione, pectin, arginine and plant extracts.

EFFECT: invention makes it possible to obtain biologically active additives in form of gel, which possess effective effect on human organism, with compositions possessing increased stability.

2 dwg, 6 ex

Description

The invention relates to medicine, in particular, considers the features of the construction of solvents for biologically active additives. In the framework of this invention we are talking about the basics of gels that use water as one of the components.

The basis of the life of the body is the balance of oxidative and reducing biochemical processes in it. Water is responsible for these processes as the main solvent - in the chemical sense - and as the main structural element of the body - in biological terms. The intensity of redox reactions depends on the activity of electrons in aqueous solutions, which is characterized by redox potential (ORP). The higher this parameter, the more “acidic” the medium, the more it is capable of breaking down intermolecular bonds and oxidizing molecules; and vice versa: the lower the AFP, the higher its regenerative ability.

The issue of the conformity of the ORP of water to the human body is taken into account in some cases in the production of medicines and cosmetics.

For example, from RU 2274465, A61K 36/07, A61K 36/25, publ. 04/20/2006, known compositions that contain active components from one or more types of Basidiomycotina belonging to the tubular Basidiomycotina, and active components from the root of the plant belonging to Araliaceae. Such compositions have hypoglycemic and antitumor activity. For these compositions, an aqueous solution with a redox potential of less than about 1230 mV, more preferably less than about 900 mV, and most preferably less than about 330 mV, is used.

It has been established that after the composition is obtained or after its dissolution or suspension, its redox potential, as a rule, changes with time. Therefore, the ORP of the liquid mixture should be checked from time to time to clarify that the ORP of the solution is approximately +900 mV or less. It has also been established that the redox potential of the resulting composition is of great importance, because, especially in the case of a composition with a redox potential of +900 mV or less, there is a negative correlation between the redox potential of the composition and its antitumor activity. In other words, the lower the redox potential, the stronger the antitumor activity of the composition. Thus, it is possible to increase the effectiveness of treatment.

These compositions contain only components extracted from Basidiomycotina and components extracted from the root of the plant Araliaceae. However, it has been found that other active or inactive ingredients may also be included in these compositions until the additional ingredients inhibit the synergistic effect of the two above-mentioned extracted components. For example, these extracted components can be mixed with pharmaceutically acceptable carriers and additives. The effect of improving the treatment manifests itself in other forms, for example, in the form of a solution, syrup, suspension, emulsion, granules, tablets, pills, capsules, creams, lozenges, chewable tablets, suppository, eye drops, injections, aerosol, elixir, etc. . Compositions in solid (powder) form must be restored by adding water.

Also identified from RU 2336880, A61K 33/00, A61P 1/00, A61P 11/06, A61P 17/00, A61P 43/00, publ. October 27, 2008, that when using means for stimulating the lymphatic drainage of drinking water that has undergone baromembrane treatment and has a redox potential of +200 to +343 mV, the total mineralization is from 25 to 130 mg / l and the pH is from 6.9 to 8 , 3, stimulation of lymphatic drainage is significantly increased in the treatment of patients with chronic skin diseases, non-oncological diseases of the gastrointestinal tract and chronic obstructive bronchitis. The first intake is carried out on an empty stomach. This tool allows you to highly systematically stimulate lymphatic drainage of body tissues by enhancing the processes of formation and transport of lymph without any undesirable irritating effects.

Water is obtained with such ORP for a means of stimulating lymphatic drainage, due to the fact that the water is preliminarily adjusted to the state of drinking water and then treated on a baromembrane unit, while water is passed through membranes, which are a semi-permeable material with a hole diameter of 0.0001- 0.005 μm, purified water has the following indicators: redox potential from +200 to +343 mV, total salinity from 25 to 130 mg / l, pH from 6.9 to 8.3. In addition, purified water can be subjected to additional processing, saturating it with macro- and microelements (calcium, magnesium, sodium, sulfur, vanadium, carbon, oxygen; iron, iodine, copper, cobalt, zinc, fluorine, selenium, silicon, silver, etc. .).

So, the ORP of the internal environment of a healthy person is always less than zero and ranges from -100 to -250 millivolts. ORP of almost any, including industrially treated drinking water, is always greater than zero and ranges from +100 to +400 millivolts. These differences in the ORP of the internal environment of the human body and drinking water mean that the activity of electrons in the internal environment of the human body is much higher than the activity of electrons in drinking water. When ordinary drinking water penetrates the tissues of the human (or other) body, it takes away electrons from cells and tissues, which are 80-90% water. As a result of this, the biological structures of the body undergo oxidative destruction. In order for the body to optimally use drinking water in metabolic processes, it must be conditioned according to the ORP indices, which is achieved, for example, on the basis of electrochemical activation (ECA) technologies. If the drinking water entering the body has an ORP close to the value of the ORP of the internal environment of the human body, then the water is immediately absorbed, since it has biological compatibility in this parameter. If drinking water has an ORP more negative than the ORP of the internal environment of the body, then it feeds it with this energy, which is used by the cells as an energy reserve of the antioxidant defense of the body from the adverse effects of the external environment.

Scientists from the Institute of Organoelement Compounds named after A.N. Nesmeyanov of the Russian Academy of Sciences and from Moscow State University named after M.V. Lomonosov in collaboration with Fiuggi water experts Eugenia Girbul measured the pH and ORP of the Fiuggi mineral water at the source and found that the ORP (Redox potential) and the pH of the mineral water in Boniface Park in one of the closest places to the exit of water from the ground at a temperature of 19 ° C ORP averaged -32.5 mV at pH 7.44. In other fountains located 100-150 m from this place, the ORP is - 27.3 mV at pH 7.38. This means that Fiuggi mineral water has reducing properties. For comparison, the redox potential of tap water in the park turned out to be equal to an average of +26.0, and pH 6.5. Those. tap water has oxidizing properties. This situation, unfortunately, is typical of tap water and is associated with the features of its treatment. The redox potential of human extracellular fluid is in the range from -100 to -200 mV at a blood pH of about 7.4. Those. the internal environment of a person, like Fiuggi's mineral water, is in a restored state. And the closer the parameters of the consumed water to the parameters of the body, the more useful the water. A negative redox potential, along with low mineralization and the presence of humic acids in the water, is another reason for the therapeutic effect of Fiuggi mineral water.

Typically, the ORP of the human body ranges from -90 mV to -200 mV, and the ORP of ordinary drinking water is almost always significantly above zero: tap water from +80 mV to +300 mV, water in plastic bottles from +100 mV to +300 mV, and well, spring water from +120 mV to +300 mV. Negative ORP of natural water is an extremely rare phenomenon. When drinking ordinary water, the activity of electrons in the internal environment of the body is higher than the activity of electrons in it. Those. such drinking water takes free electrons from the biological environment of the body, i.e. is an oxidant. This leads to premature aging, chronic diseases, chronic fatigue. Conversely, a negative ORP of drinking water gives energy to cells, organs, and systems. The electrical energy of cell membranes is not spent on the correction of the activity of electrons of water and water is immediately absorbed, because possesses biocompatibility in this parameter. Negative ORP drinking water is an ideal antioxidant.

The second evidence of the benefits of low ORP water for the human body is information about the properties of melt water. Despite the fact that melt water has been known for several centuries, interest in it is due to its benefits for health and beauty, the ability to rejuvenate the body and prolong life. In fact, melt water is almost perfectly pure and is no different from distilled water. The composition of melt water does not include any macro- and microelements, including those useful for the body. The only exception is a change in its formula with redox potential. Melt water in its composition and structure resembles human blood (in some sources they write that it resembles blood plasma), which is why it is very useful. In fact, only saline can be compared with blood plasma. It is this artificially prepared solution that approaches the plasma composition in terms of salt composition and osmotic pressure. The most popular advice regarding the use of melt water is to wash with pieces of ice, which "refreshes and rejuvenates the skin of the face." It really is. However, the anti-aging effect is explained not so much by the composition of melt water, but by its temperature, mostly very low. Short-term exposure to cold improves blood circulation and gives the skin a healthy look. Beauticians recommend periodically wiping the skin on the face with ice cubes.

The prototype of our invention is the use of water with a stable negative redox potential for the manufacture of, including biologically active additives, while sodium bicarbonate and potassium bicarbonate are dissolved in water so that the ratio of sodium to potassium ions in water is 2-6: 1 (RF Patent 2479318, published April 20, 2013).

However, the manufacture of this tool has the following disadvantage:

1. The addition of salts to the composition of the product to reduce the ORP of the solution changes the effect of the active substances in their composition.

The present invention is aimed at achieving a technical result, which consists in increasing the effectiveness of exposure to the human body of water-based gels for dietary supplements, as well as improving their stability.

The specified technical result is achieved by using water with a negative redox potential of less than zero mV of the claimed range as the basis for biologically active additives in the form of gels.

These signs are essential to achieve a technical result.

In FIG. Figure 1 shows a graph of the change in the ORP in an open container: a decrease in the ORP when purging with hydrogen through the upper water layer, the total volume of water is 1 liter The hydrogen purge rate of 1 l / h. After 24 hours, the ORP becomes minus 450 mV.

In FIG. Figure 2 shows a graph of changes in redox potential in an open dish: an increase in redox potential saturated with hydrogen when stored in an open dish with a liquid volume of 250 ml.

In the framework of this invention we are talking about the basics of gels, that is, soft forms intended for application, including on the skin or wounds and mucous membranes and consisting of a base containing water as one of the components, and biologically active substances uniformly distributed in it . Preservatives, surfactants and other excipients authorized for medical or other use can be introduced into the gels themselves.

In the framework of the present invention will be considered hydrophilic bases, that is, able to mix with water or dissolve in it. This type also includes substances that give stable gels after swelling in water with subsequent dissolution (solutions and gels of polysaccharides, proteins, oligoesters, polyethylene oxide bases, etc.), substances insoluble, but swellable in water (phytosterol and sitosterol gels, hydrophilic clay minerals).

The authors conducted studies on the use of water with low (negative) AFP to increase the effectiveness of dietary supplements (dietary supplements) in the form of gels in which water was used as a base component.

The main processes that ensure vital activity are redox reactions. The energy released during these reactions is spent on the regeneration of body cells and maintaining homeostasis relative dynamic constancy of the composition and properties of the internal environment. In the course of redox reactions, the electric potential of substances changes: one substance gives off its electrons and oxidizes, acquiring a positive charge, the other accepts electrons and, being charged negatively, is restored. The difference in electrical potentials between them is the redox potential (ORP).

ORP, which is also called the redox potential, characterizes the degree of electron activity in redox reactions. The greatest oxidizing ability is oxygen, and the reducing ability is hydrogen.

Differences in the ORP of the internal environment of the human body and drinking water mean that the activity of electrons in the internal environment of the human body is much higher than the activity of electrons in drinking water. If the fluid entering the body has an ORP close to the value of the ORP of the internal environment of the human body, then the electrical energy of the cell membranes (the vital energy of the body) is not spent on correcting the activity of the electrons of this fluid, and it is immediately absorbed, since it has biological compatibility in this parameter. If the incoming liquid has an ORP more negative than the ORP of the internal environment of the body, then it feeds it with this energy, which is used by the cells as an energy reserve of the antioxidant defense of the body from the adverse effects of the external environment.

To confirm the hypothesis about the possibility of enhancing the activation of biologically active additives by using water with a low (below zero mV) ORP as a direct filler or a base or component of an aqueous solvent, studies were conducted on the stability of low ORP water, at least for a given period of time (e.g. up to three days).

The measurement results of the characteristics of the water prepared by the methods of RU 71331 and 71332 showed the following.

In non-hydrogenated water, the content of dissolved oxygen decreases in 40 days from 13 to 7.9 mg / l, the potential decreases from 570 to 120-150 mV. In the water that is charged immediately, the oxygen content decreases strongly, after 40 days there is almost no oxygen left, the ORP drops to -350 mV. In the settled water, the concentration of dissolved oxygen also decreases, but with a long exposure time (11-40 days), oxygen in the solution remains noticeably larger. The ORP of water immediately charged with hydrogen decreases more slowly than in stagnant water, although it reaches approximately the same values. In the settled distilled water, characterized in that it has a lower concentration of dissolved oxygen, and during its processing there are no stages when active particles can form, after 1 hour (0.041 days) the ORP has decreased to - 170 mV, although the concentration of dissolved oxygen has not yet has changed. It clearly shows a tendency to decrease ORP more rapidly and achieve lower potential values. It is noteworthy that in the water treated with cold plasma after long standing (40 days), the concentration of dissolved oxygen in the water is almost the same as in distilled, although the potential is much lower. Over 5 hours (0.21 days) in the first three types of water, the potential decrease was 250-350 mV, while in distilled water the potential decreased by ~ 700 mV.

, которая близка к нулю. С понижением концентрации кислорода ОВП раствора будет уменьшаться. Содержание кислорода уменьшается при его расходовании на окисление водорода.When water is saturated with hydrogen and oxygen and equilibrium is established, the solution potential will be determined by the sum of the equilibrium potentials $$j_{\mathrm{one}}^0(pH=7)+{\mathrm{<figure-callout\; id="2"\; label="j"\; filenames="00000013.png,00000014.png"\; state="\{\{state\}\}">j</figure-callout>}}_2^0$$

which is close to zero. With decreasing oxygen concentration, the ORP of the solution will decrease. The oxygen content decreases when it is spent on the oxidation of hydrogen.

Thus, we can say that the slow establishment of the potential in the presence of dissolved oxygen and hydrogen in water is due to the consumption of oxygen in the reaction with hydrogen. If the amount of dissolved hydrogen is greater than is necessary for the reaction with oxygen, then after spending all the oxygen in the water, hydrogen remains, which determines the value of the redox potential. It should be emphasized that if water is saturated with hydrogen and oxygen to the limit of solubility, there will always be enough hydrogen to completely consume oxygen, and a negative ORP will be obtained.

In the case of electrolysis, dissolved oxygen instantly reacts at the cathode with atomic hydrogen, and the oxygen concentration in the catholyte is zero. The formation of OH ^- ions in catholyte increases the pH value; therefore, during the electrolysis of redox potential, catholyte will always be less than just when saturated with hydrogen, and this difference can be of the order of 400 mV if neutral water was electrolyzed.

The observation of reduction reactions in aqueous solutions saturated with hydrogen, in particular, the reduction of molecular iodine, manganese, and iron compounds, shows that the measured value of the potential is due not only to the electrode processes on platinum, but to the real properties of the solution. To restore the compounds in solution, hydrogen is consumed. The recovery process stops after the complete consumption of hydrogen, if it is not enough to recover all substances, and the ORP of the solution does not change. When the concentration of hydrogen with a guarantee exceeds the stoichiometrically necessary amount for reduction, hydrogen remains and the potential of the solution decreases.

I did not find a direct explanation for the effect of a slow increase in the redox potential when opening the surface of water saturated with hydrogen. A light gas, hydrogen should diffuse instantly from water, since there is very little hydrogen in the atmosphere above its surface. However, if we imagine water as a network bonded with hydrogen bonds, then the introduced hydrogen can be fixed by hydrogen bonds, forming molecules of a more complex composition than H ₂ O.

The dependence of the ORP on the volume of a gas bubble remaining above the surface of the water after the introduction of hydrogen can be explained by a decrease in the partial pressure of hydrogen spent on the reaction with oxygen. As was shown, the process of oxygen consumption is slow, it can take several days or more. Therefore, the partial pressure of the initially introduced hydrogen gradually decreases, and the larger the volume of the initial bubble, the weaker the decrease in the amount of hydrogen in the bubble, and the pressure decreases less. The concentration of oxygen dissolved in water in the experiments on finding the dependence of the ORP on the volume of the gas bubble was the same in all samples, and the absolute amount of oxygen in the sample decreased with increasing hydrogen bubble, because the volume of water decreased.

The kinetics of establishing ORP in an open vessel of 1 liter volume when hydrogen is bubbled through a surface layer 3–4 mm thick is shown in FIG. 1. The hydrogen feed rate was 1 l / h. From figure 1 it is seen that one hour after the start of the purge ORP reaches a value of minus 150 mV, after 2.5 ÷ 3 hours, approximately minus 300 mV. After a day, the purge is set ORP = -450 mV.

The kinetics of increasing ORP during storage of hydrogen-saturated water in an open vessel with a volume of 250 ml is shown in FIG. 2. The initial value of the ORP minus 520 mV. Over 6 hours, the ORP increases to minus 380 mV. Then within three days, the ORP increases to minus 100 mV.

Thus, based on the experiments, the following conclusions were obtained:

1. The potential establishment time in a bottle filled with hydrogen is approximately 1 day.

2. The value of the established potential depends on the amount of hydrogen introduced: the potential increases with increasing volume of hydrogen in the bottle and tends to reach a plateau.

3. The pH value of distilled water, simply purified water and all investigated aqueous solutions does not change when saturated with hydrogen.

4. The value of the potential established after 24 hours depends little on the type of dissolved substance and its concentration if the dissolved substance does not interact with hydrogen.

5. The increase in ORP during storage of the initially hydrogenated solution is associated with the leakage of hydrogen from the bulk. If there is no hydrogen leak, no increase in potential is observed.

6. The main role in lowering the ORP in the studied processes is played by hydrogen dissolved in water. The contribution of other processes, which can also lead to a decrease in redox potential, is much less.

After obtaining data on the ORP stability of water, preparations were prepared based on water with an ORP below zero (-201 - 225 mV).

Studies have been conducted on the use of water with AFP from -215 mV for disinfection and sterilization of instruments, rooms, equipment, care items, skin and mucous membranes, etc. Tests have shown that they, when exposed for 5-10 minutes to rinse the oral cavity, reduce the seeding by microorganisms of the oral cavity and pharynx by 25-100 times, which is confirmed by their successful use for rinsing with diseases of the pharynx (L.G. Bazhenov et al., 1999) . The use of wetted wipes allows you to completely clean the wound cavities with gunshot wounds, phlegmon, abscesses, trophic ulcers, mastitis, extensive purulent-necrotic lesions of the subcutaneous tissue in 3-5 days, and subsequent use within 5-7 days significantly accelerates the reparative processes. There is also evidence of the high therapeutic efficacy of electroactivated solutions for non-specific and candidal colpitis, endocervicitis, residual urethritis, cervical erosion, corneal ulcers, purulent keratitis, infected wounds of the eyelids, for the correction of dysbiosis and immune disorders, in the treatment of stomatitis, gingivitis, parade with diseases of the stomach; in the treatment of salmonellosis, dysentery, as well as in the treatment of diabetes mellitus, tonsillitis, purulent otitis media, oily and dry seborrhea of the face, hair loss, contact allergic dermatitis, wrinkle correction. A good effect was found when using water with an AFP of 215 mV for gastritis, peptic ulcer, hemorrhoids, dermatomycosis, eczema, prostate adenoma and chronic prostatitis, tonsillitis, bronchitis, chronic pyelonephritis, chronic hepatitis, viral hepatitis, deforming arthrosis, etc. .

The authors conducted a study on the antiviral activity of a stabilized solution of a base containing water with reducing properties in experimental infections caused by hepatitis C virus in cell cultures, showed that:

- SVRVS (stabilized aqueous solution with reducing properties, ORP = -210 - -220 mV) does not have cytotoxic properties for Vero-E6, SPEV, PTP cell cultures for 4 or more days of cultivation.

- SIRS has virucidal activity against hepatitis C virus (HS) upon exposure to HCV - containing fluid and medium. A needle prepared on SIDS for 1 hour and 24 hours; HCV titers for cell cultures are reduced by 2.3-3.0 lg TCD50 .

- SVRVS has antiviral properties and is able to reduce the production of HCV-infected adolescents by an average of 3.0-4.0 lg when added immediately after adsorption of HCV on the cells or 24 hours before hepatitis C virus infection of the cells.

These results clearly show the possibility of using water with an ORP below zero mV as the basis for dietary supplements in the form of gels.

Below are compositions with ORP from -201 to -225 mV. Observations of the compositions of the examples were carried out for more than 6 months (at room temperature), while the value of the ORP practically did not change. A study was conducted using artificial aging of the product, which showed that the AFP does not change for 18 months of the estimated time.

The gel, including that used for dietary supplements, is a high viscosity product based on water (70-80%). Also, the gel may contain glycerin (10-30%), gelatin (2-3%), gum arabic, starch, agar-agar, pectin, trialose (a natural polysaccharide based on corn starch, which has a very high water-holding capacity). The gel does not contain oils and fats, and therefore it has a more delicate and lighter consistency, is absorbed faster and does not leave a greasy film on the skin. Due to these properties, the gel is perfect, for example, for oily, young, problematic skin, fits well under makeup. With gel, the skin breathes better. This tool has excellent moisturizing properties, so it is sometimes applied directly under the cream, giving a more nourishing, protective effect.

Given these features, the authors tested gels, which were based on purified water with a negative redox potential of less than zero mV. In this case, water with a negative redox potential of (-201) - (- 225) millivolts was used.

Using water with negative AFP, the authors developed functional complexes that have a restorative and preventive effect on the key life processes of the human body for its restoration, rejuvenation and enhancement of its biological properties and vital signs. Each of the complexes is based on a unique combination of amino acids, including glutathione and arginine, with complexes of natural plant extracts and vitamins.

The following gel composition was used as bases with water: water (70-80%), gelatin (2-3%) with additives from the series: gum arabic, starch, agar-agar, pectin, trialose. Any other composition having water may be used.

One of the main components of each complex is glutathione, which determines the redox status of the intracellular environment and keeps it in a stable range. Glutathione - g-glutaminyl-cystsinyl-glycine, a tripeptide formed by the residues of three amino acids - glutamic acid, cysteine and glycine. The structural feature of glutathione is a peptide bond between cysteine and glutamic acid, in which its g-carboxyl group is involved. Glutathione is found in all living organisms and is important for redox reactions due to the ability of the sulfhydryl group (SH-) cysteine to enter a reversible reaction. Apparently, the functions of glutathione in metabolism also include the protection of SH groups of cytoplasmic proteins from oxidation. Thanks to glutathione, the ORP index in the complexes (-200) - (- 240) millivolts (mV). Thus, glutathione creates an active, workable, favorable environment for each cell. Glutathione is a key element of antioxidant protection: it is able to restore other antioxidants. The main antioxidant role of glutathione is to protect immune cells, primarily lymphocytes. If glutathione is not enough, lymphocytes die and the functioning of the immune system is seriously impaired, which leads to a number of diseases. Glutathione contains sulfur-containing groups. Sulfur is a sticky substance, and all the “debris” contained in the body adheres to its molecules, including free radicals, toxins and heavy metals. Glutathione is a protein that is synthesized in every cell of the human body.

Glutathione needs to be replenished by everyone, even very healthy people, starting from the age of 20. Since it is from this age that the synthesis of glutathione by the body begins to decline (approximately 1% per year). The older a person becomes, the more his body loses its ability to produce enough glutathione. This makes a person susceptible to uncontrolled cell breakdown due to oxidative stress and the growth of free radicals, defenseless against infections and many immune diseases. Glutathione deficiency occurs in so many diseases. These include chronic fatigue syndrome, cardiovascular disease, cancer, chronic infections, autoimmune diseases, diabetes, autism, Alzheimer's disease, Parkinson's disease, arthritis, asthma, kidney and liver diseases, and many others.

Arginine - 2-amino-5-guanidinovaleric acid, diaminomonocarboxylic amino acid, conditionally essential amino acid. In an adult and a healthy person, arginine is produced by the body in sufficient quantities. At the same time, in children and adolescents, in elderly and sick people, the level of arginine synthesis is often insufficient. In this case, a certain amount of arginine should come with food. Physiologically significant for the body is the levorotatory form of arginine - L-arginine. All its pharmacological effects are associated with conversion to nitric oxide (NO) molecules. Nitric oxide, in turn, takes part in the regulation of blood circulation, immunological and neurotransmitter functions, liver function, blood coagulation and sexual function.

Arginine is part of many proteins and is one of the precursors in the synthesis of creatine;

an intermediate product of the synthesis of urea in the liver;

enhances the detoxification of xenobiotics and promotes detoxification and elimination of ammonia;

takes an active part in the regulation of metabolism in the body;

activates the regeneration processes in the post-traumatic period during the healing of fractures, in case of burns (restores the protein balance in severe burns), in the healing of trophic ulcers;

participates in collagen formation processes;

is a donor of the multifunctional signaling molecule of nitric oxide (NO), the discovery of the biological effects of which was awarded the Nobel Prize in medicine;

is part of the peptide hormone pituitary vasopressin;

promotes the functioning of the thymus gland (thymus), increases its size and activity;

performs important immune functions, participating in the formation of antibodies, stimulates the production of T-lymphocytes;

increases the antibacterial activity of neutrophils;

increases the content of growth hormone in the blood;

reduces body fat;

promotes the synthesis of glycogen in the liver and muscles;

increases the level of glucagon, prolactin, ornithine, arginine phosphate, etc .;

increases spermatogenesis - participates in the formation and formation of seminal fluid (almost 80% of its volume);

improves the quality and duration of an erection, enhances sexual desire in both sexes, increases the frequency and intensity of orgasms in women;

regulates blood pressure;

possesses antitumor activity (when arginine is converted to nitric oxide (NO) - a free-radical compound that destroys tumor cells);

NO inhibits the development of osteoporosis and helps to increase bone density;

preventive remedy for physical and mental fatigue;

prevents premature aging.

It has been clinically proven that arginine in combination with carnitine can stimulate the formation of growth hormone in the human body: there is a decrease in subcutaneous fat and an increase in muscle mass.

Complexes of plant extracts and vitamins make up for the lack of biologically active compounds, contribute to the restoration and improvement of the body.

All plant extracts and vitamins that make up each complex are of natural origin, natural and selected in such a way that each complements and enhances the effect of each other, as a result of which the composition works to the maximum extent possible.

Pectin is necessary to stabilize the metabolism, reduces cholesterol in the body, improves peripheral blood circulation, as well as intestinal motility. He has the ability to clean living organisms from harmful substances without disturbing the bacteriological balance of the body. Its enveloping and astringent properties favorably affect the condition of the mucous membrane of the gastrointestinal tract.

It is the combination of glutathione + arginine + plant extracts and vitamins against a negative ORP of water from -201 to -225 mV that provides the maximum healing effect on the body and protection against the destructive effect of free radicals.

Below are examples of complexes whose component composition is selected experimentally.

Complex No. 1 is a peptide complex, normalizes the functioning of the cardiovascular system, and reduces the risk of cardiovascular diseases. Helps to restore functional disorders of cardiac activity and heart rhythm, normalize blood pressure, improve heart muscle function, increase blood circulation in the coronary vessels. Helps to normalize cholesterol metabolism and prevents the formation of blood clots in blood vessels, strengthens capillaries. Provides powerful antioxidant protection at the cellular level. It normalizes the state of the immune system and helps to improve blood circulation in blood vessels and the brain, reduce the excitability of the central nervous system, and relieve fatigue.

Peptide complex (glutathione, alanine, glutamic acid, aspartic acid, arginine) - determines the redox status of the intracellular environment, has a powerful antioxidant, thrombolytic, anti-atherosclerotic, vasodilating effects.

Red wine extract - contains quercetin, rutin, anthocyanins, catechins, resveratrol, micro and macro elements, essential amino acids, vitamins, organic acids. It helps to improve the condition of the aorta and lower cholesterol, widen the coronary vessels, lower blood pressure and lower blood viscosity. Prevents blood clots.

Hawthorn fruit extract is a source of flavonoids, the amount of which reaches 17, trace elements Cu, Fe, Zn, Mg, Ca. They have a vasodilating effect on the blood vessels of the heart, promote the assimilation of oxygen by the heart muscle.

Cranberry fruit extract - contains bioflavonoids, catechins and phenolic acids, as well as betaine and important micro and macro elements: potassium, calcium and phosphorus. Strengthens the muscles of the heart, increases the strength of capillaries, prevents the formation of blood clots in blood vessels, and improves blood circulation in blood vessels and the brain.

B vitamins (B1, B6, B12 - thiamine hydrochloride, pyridoxine hydrochloride, cyanocobalamin) - help strengthen the cardiovascular and nervous system, improve blood formation, support the body with prolonged overload and stress.

Apple pectin - stabilizes metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

The experiments were carried out on 45 fragments of the kidneys of Wistar rats weighing 150–200 g. The studies were carried out by administering massive doses of catecholamines to the animals, which leads to an excessive stimulating effect of the heart muscle with a relative lack of blood and oxygen supply, which causes heart-like changes in the heart ECG changes and the appearance of arrhythmias. The use of the composition before introducing the stimulator confirmed the energy-saving effect of the composition, which was manifested in the inhibition of glycolysis, increasing the efficiency of oxidative phosphorylation in mitochondria. As a result of the action of the composition during hypoxia and ischemia, glycogen stores are not depleted, the activity of LDH, LDH decreases, the activity of NADH-DG is preserved.

The gel also showed its stability for 6 months while maintaining the properties of water with negative ORP within the stated range.

Complex No. 2 - the peptide complex provides reliable protection of the kidneys and urinary system. It prevents the formation of stones in the kidneys and bladder, has a bactericidal effect in urinary tract infections. Helps to reduce inflammatory processes in the kidneys and bladder, urinary stagnation phenomena. It has a diuretic effect, helps soften and remove stones and salts. Normalizes the synthesis of uric acid and promotes its excretion from the body. Increases detoxification capabilities of the body, has a restorative effect. The skin condition is restored, excessive puffiness goes away, the manifestation of cellulite is reduced.

Peptide complex (glutathione, glutamine, arginine) - determines the redox status of the intracellular environment, has a powerful antioxidant effect.

Rosehip extract with vitamin C contains ascorbic acid, riboflavin, carotene, phylloquinone and bioflavonoids (vitamin P).

Soya lecithin is necessary for the body as a building material for the renewal of damaged cells, the prevention of stone formation, and has a general strengthening effect.

Cranberry and lingonberry extracts have diuretic and antimicrobial properties, relieve the condition with urolithiasis and urinary tract inflammation, and prevent the formation of kidney stones.

Juniper extract contains vitamins, sugars, pectin, organic acids. Provides anti-inflammatory, bactericidal and diuretic effects. Helps to remove pathogens from the body.

Pineapple extract provides decongestant action.

Celery extract contains zinc, potassium, calcium, phosphorus, iron, magnesium, vitamins of groups B, PP, E, A. Prevents the appearance of kidney stones, helps to eliminate decay products, normalizes pressure.

Apple pectin stabilizes the metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

The effect of the drug on the development of kidney explants. The experiments were carried out on 45 fragments of the kidneys of Wistar rats weighing 150–200 g. The composition was added to the nutrient medium at a concentration of 2 ml / 100 g. The area index (PI) —the ratio of the area of the entire explant together with the growth zone — served as a biological activity criterion to the outgoing area of the kidney fragment. The IP values were expressed as a percentage, the control IP value was taken as 100%. It was established that after 2 days of cultivation, the explants spread on a collagen substrate and the proliferation of proliferating and migrating cells began on the periphery of the explant. On the 3rd day of cultivation, at a drug concentration, a significant increase in the explant PI by 28% was observed compared with the control PI values. Thus, in relation to kidney tissue, the drug exerted a tissue-specific effect, manifested in stimulating the growth of explants.

The gel also showed its stability for 6 months while maintaining the properties of water with negative ORP within the stated range.

Complex No. 3 - the peptide complex helps to normalize the function of the liver, biliary tract and gastrointestinal tract. It helps to restore the functioning of the liver and biliary tract, increases the production of bile and prevents its stagnation with dyskinesia of the biliary tract, helps dissolve stones in the gall bladder. It has a hepatoprotective property, i.e. protects the liver cell from various lesions of toxins, from viruses, decay products, removes toxins from the body. It has anti-allergic, antiseptic, anti-inflammatory properties, a slight laxative property. It normalizes bilirubin metabolism and the level of cholesterol in the body involved in the formation of prothrombin in the liver, which is necessary to maintain normal blood coagulation in the body.

Peptide complex (glutathione, glucamine, arginine) - determines the redox status of the intracellular environment, initiates the production of detoxification enzymes in the liver, protects the cells of the liver itself from damage, and promotes their regeneration.

Green tea extract - a source of catechins, vitamins C, Bl, B2, PP, K, amino acids and trace elements, has a hepatoprotective effect, preventing structural changes in the tissues of organs, their growth.

Soya lecithin - protects the liver from the effects of preservatives, insecticides, toxins, drugs, hormones, etc. It is necessary for the formation and stabilization of the biological structure and regeneration of the membranes of the liver cells.

Lipoic acid - protects the liver, restores the integrity of liver cells. It prevents fatty degeneration of the liver and removes excess fat from the liver. Reduces glutathione consumption.

Oat extracts - contains amino acids: tryptophan and lysine. As well as gum and vitamins: B1, B2, B6, vitamin K, carotene and acids (oxalic, erucic, molonic), magnesium and potassium, chromium and phosphorus, manganese, iron, nickel, iodine, fluorine and zinc, sulfur. Helps cleanse the liver. It has anti-allergic, antiseptic, anti-inflammatory properties, a slight laxative property.

Blackcurrant extract - contains vitamins C, B1, B2, B6, B9, E, K, P, A, D. Pectins, carotenoids, salts of phosphorus, iron and potassium.

It has an auxiliary, anti-inflammatory effect. Helps dissolve gallstones. Antitumor protection.

Melon extract contains potassium, vitamins PP, B, C, carotene, phosphorus, copper, silicon, magnesium, calcium, organic acids, carbohydrates.

Helps restore liver function, helps dissolve gallstones.

Celery extract contains proteins, vitamins, amino acids and minerals. Ensures the stability of body cells and slows down the aging process. Reduces fat deposition in the liver.

Orange extract has a choleretic effect.

Vitamins B4, B9 prevent obesity of the liver, restores liver tissue damaged by toxic drugs, viruses, alcohol and drugs.

Apple pectin - stabilizes metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

The experiments were performed on fragments of the liver of Wistar rats weighing 150-200 g. Liver fragments were placed in this medium and cultured in Petri dishes in an incubator at a temperature of 36.7 ° C for 2 days. The peptide composition was added to the experimental medium at concentrations of 2 ml / 100 g. The area index (PI), the ratio of the area of the entire explant together with the growth zone to the outgoing area of the liver fragment, was used as a criterion of biological activity.

It was established that after 2 days of cultivation, the explants spread on a collagen substrate and the proliferation of proliferating and migrating cells began on the periphery of the explant. There was a significant increase in the explant PI by 40%, compared with the control PI values, which indicates a tissue-specific effect, manifested in stimulating the growth of explants.

The gel also showed its stability for 7 months while maintaining the properties of water with negative ORP within the stated range.

Complex No. 4 - the peptide complex contributes to the normalization of respiratory function. The respiratory system consists of the nose, pharynx, trachea, bronchi and lungs. It has a bactericidal effect in infections of the upper respiratory tract. It has an expectorant, antitussive, anti-inflammatory, antifungal, antiviral effect. Helps to restore the function of the bronchopulmonary system, reduce inflammation in the bronchi, trachea, lungs, restoring the functional state of the mucosa. It has a resolving, healing, diaphoretic and antipyretic, tonic and stimulating effect. It has an immunomodulatory effect.

The peptide complex (glutathione, arginine, glutamine, aspartic acid, leucine) - determines the redox status of the intracellular environment, has a powerful antioxidant, healing and regenerating effects.

Blackcurrant extract is a powerful anti-inflammatory effect, as it is a source of natural antibiotics and phytoncides, which kill fungi, bacteria, viruses in the body, and a high content of ascorbic acid increases immunity.

Extracts of mango, ginger root, lingonberry - sources of vitamins: A, B1, B2, PP, B5, B6, B9, C, E. They contain minerals: potassium, calcium, phosphorus, magnesium, sodium, iron, copper, zinc, manganese, sulfur. Provide a pronounced anti-inflammatory effect, dilutes sputum and promotes its easy passage from the bronchi, increases immunity.

Juniper extract - contains many tannins, organic and ascorbic acids. Provides bactericidal effect. It removes phlegm. Helps to restore the functional state of the bronchial mucosa.

Extracts of raspberries, oranges - contain vitamins C, B1, B2, P, K, E, B9, carotene. It has a bactericidal effect in infections of the upper respiratory tract, as well as diaphoretic and antipyretic, tonic effects.

Vitamins B6 and B12 - increase oxygen consumption by cells, which helps to eliminate hypoxia that occurs in acute and chronic bronchitis, and also facilitates its course.

Apple pectin stabilizes the metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

The tissue explants of lungs of mature Wistar rats were cultured in Petri dishes with a collagen-coated bottom. The test composition was added to the culture medium at a concentration of 2 ml / 100 g of culture medium. The same volume of 0.9% NaCl solution was added to the control group explants. After incubation for 3 days at a temperature of 37 ° C, an increase in the area of the explants was evaluated under a phase contrast microscope. The biological effect of the composition was evaluated by changing the area index (PI) of explants grown in a medium with a peptide, compared with the control. The results of the experiment showed that the composition significantly stimulates the growth of explants of organotypic culture of lungs of rats more than 50%.

The Gel complex also showed its stability for 6 months while maintaining the properties of water with negative ORP within the stated range.

No. 5 - the peptide complex contributes to the normalization of the digestive system. Digestive system: oral cavity, pharynx and esophagus, stomach, duodenum - the initial section of the intestine, small intestine, large intestine, digestive glands - liver, pancreas, salivary glands. Helps normalize the functional state of the stomach, duodenum, liver, pancreas, small and large intestines. It improves the composition of the intestinal flora, reduces pain and inflammation, reduces secretion, normalizes intestinal motility and improves the absorption process. Helps to improve intestinal motor function. Eliminates the main symptoms of diseases of the digestive system, normalizing acute and chronic processes that turn into a stable remission.

Peptide complex (glutathione, glutamic acid, arginine) - determines the redox status of the intracellular environment, has a powerful antioxidant, healing and regenerating effects.

Glycine - slows down the degeneration of muscle tissue, is necessary for the synthesis of nucleic acids (DNA and RNA). Useful for repairing damaged tissue.

Vitamin PP - promotes the breakdown of proteins, fats and carbohydrates, thereby helping the stomach easier to digest food.

Melon extracts contain vitamins A, B1, B2, B3, B4, B5, B6, B9, C, E. Minerals: iron, potassium, calcium, magnesium, manganese, copper, sodium, selenium, phosphorus, fluorine, zinc. It normalizes intestinal function, helps restore intestinal motor function, and provides detoxification processes.

Green tea extract contains tannin, catechins, antioxidants - help to improve the digestive process and normalize the processes of the gastrointestinal tract. It has an antimicrobial effect, removes radioactive substances from the body.

Ginger root extracts are a source of vitamins: A, B1, B2, PP, B5, B6, B9, C, E. It contains minerals: potassium, calcium, phosphorus, magnesium, sodium, iron, copper, zinc, manganese, sulfur. Improves the composition of microflora. Promotes the digestion of heavy animal proteins, removes toxins and other harmful substances. Promotes weight loss.

Cranberry fruit extract - contains iron, iodine, calcium, magnesium, etc. Vitamins: B1, B2, K, PP, C, benzoic, malic, citric acids, antioxidants. Stimulates pancreatic function. Improves metabolism and immune defense, slows down the aging process.

Oat extract - contains vitamins A, B1, B2, B3, B6, K and F. Organic acids, essential amino acids, trace elements: potassium, magnesium, sulfur, chromium, iron, fluorine, iodine, manganese, zinc, nickel, phosphorus. It contributes to the normalization of acute and chronic processes in the stomach and pancreas, small and large intestines.

Broccoli sprout extract - contains ascorbic acid, vitamins A, E, vitamins of group B, PP, potassium, calcium, chromium, phosphorus, iron, magnesium, manganese, copper, iodine. Helps to eliminate belching, heartburn, nausea. Improves performance, helps cleanse the digestive system.

Soy isoflavones - contribute to the strengthening of anti-inflammatory, healing processes, normalization of the motor activity of the gastrointestinal tract, and have an immunostimulating effect.

Folic acid - has a beneficial effect on fat metabolism in the liver.

Apple pectin - stabilizes metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

A study of the biological activity of the composition on the mitochondrial mechanisms of apoptosis in gastric epithelial cells induced by the Helicobacter pylori microbe was carried out on SGC7901 human gastric epithelial cells. Cells were passaged in culture medium for 5 days, after which they were taken for research.

On the 5th day after the start of cell passage, the composition was introduced into the culture medium at a concentration of 2 ml / 100 g, and after 30 min of incubation, the cells were taken for examination. As a control, cells were considered without adding a composition. The condition of isolated mitochondria was assessed using immunofluorescence confocal laser microscopy. Under the action of the composition, the outlines and structure of mitochondria were preserved, the expression of mitochondrial GFP practically does not differ from the initial indicators (2.48 ± 0.17 cu in optical density; 3.84 ± 0.20% in expression area), which indicates the property of preventing mitochondrial apoptosis disorders, arising under the influence of Helicobacter pylori.

The gel also showed its stability for 5 months while maintaining the properties of water with negative ORP within the stated range.

Complex number 6 - the peptide complex regulates the work of the male reproductive system. Helps to restore fertilization processes, prevention and treatment of male infertility. Normalizes function and provides support for the prostate gland. Supports the normal functioning of the genitourinary and reproductive systems. It harmonizes the nervous and endocrine systems and improves vitality. It maintains the full reproductive function of men. Supports the normal functioning of the urinary system. It has an anti-inflammatory effect, pain and inflammation decrease, and restores potency in men.

Peptide complex (glutathione, lysine, glutamic acid, arginine, proline) - determines the redox status of the intracellular environment, has a powerful antioxidant, healing and regenerating effects.

Red wine extract - contains quercetin, rutin, anthocyanins, catechins, resveratrol, micro and macro elements, essential amino acids, vitamins, organic acids. It increases the production of testosterone in men by 2.7 times, promotes the harmonization of the nervous and endocrine systems, increases vitality, a powerful antioxidant defense.

Vitamin PP (nicotinic acid or vitamin B3) is extremely important for men's health.

Mango extract - contains vitamins A, C, B6.

Walnut extract - rich in vitamins A, B1, B2, B3, B12, C, K, E, PP. It contains minerals - phosphorus, potassium, magnesium, calcium, sulfur, iron, aluminum, manganese, zinc. It enhances the synthesis of male sex hormone - testosterone, enhances the production of sex hormone, helps restore male potency.

Melon extract - contains potassium, calcium, sodium, iron, magnesium, phosphorus, vitamins B9, C, A, pectins. It contributes to the normal functioning of the genitourinary and reproductive systems, normalization of the function of the prostate gland.

Celery extract contains vitamin C, vitamins of groups B, E, A, PP, K. Minerals of zinc, magnesium, calcium, as well as potassium, iron, phosphorus, male sex hormone - androsterone, which enhances the appearance of secondary sexual characteristics and increases potency. It is a prophylactic against prostatitis. Thanks to its diuretic properties, it helps to cope with infectious diseases of the genitourinary system and reproductive organs.

Pineapple stalk and fruit extract - contains vitamin A, C, PP, B vitamins, iron, calcium, copper, iodine, potassium, magnesium. It has anti-inflammatory effect, restores male power.

Apple pectin - stabilizes metabolism, lowers cholesterol in the body, improves peripheral blood circulation. It cleanses the body of harmful substances without disturbing the bacteriological balance of the body.

We studied the contractility of isolated preparations of the prostate gland of guinea pigs at a concentration of 2 ml / 100 g. It was shown that Protethion 7 significantly increased the response of the smooth muscle of the prostate to electrical stimulation, and caused a significant increase in the amplitude of phase-tonic contractions. This effect of the drug enhances microcirculation and improves the outflow of secretion from the prostate gland. Such an action seems to be very important for the correction of disorders accompanying any changes in blood circulation and trophism of the prostate gland (and, consequently, functionally and anatomically connected organs) caused by stagnation of blood in the small pelvis, dysrhythmia of sexual life, hypothermia, trauma to soft tissues of the perineum, etc. d.

The gel also showed its stability for 4.5 months while maintaining the properties of water with negative ORP within the stated range.

Claims (1)

The use of water with a stable negative redox potential from -201 to - 225 mV as a solvent for the manufacture of biologically active additives in the form of gels containing glutathione, pectin, arginine and plant extracts.

Images