RU2556201C2 - Oil protective composition - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to application of composition, which contains Polygonum aviculare grass 20-25 wt %, cowberry leaves 13-17 wt %, Viburnum sargentii koehne fruits 5-9 wt %, fruits of dog-rose of different of Far-East species 20-25 wt %, Schisandra chinensis seeds 13-17 wt %, Fragaria orientalis leaves 5-9 wt % and corn stigmas 5-9 wt %, as means with oil protective activity in treatment of glomerulonephritis.

EFFECT: invention provides positive influence on basic indices of glomerulonephritis: promotes recovery of diuresis, reduction of proteinuria, reduction of azotemia, cryoglobulinemia and level of circulating immune complexes.

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Description

The invention relates to medicine: to pharmacology, pharmacognosy, namely, to the creation of herbal remedies that can be used as a nephroprotective agent for non-infectious chronic inflammation of the kidneys, namely glomerulonephritis.

The closest analogue prototype is a herbal preparation, which contains 100 g of centaury herb 0.6 g, lovage 0.6 g of roots, rosemary leaves 0.6 g, purified water 71.0 g, ethanol content 16 , 0-19.5% - in volume terms under the name Kanefron N, oral solution (Instructions for use of the medicinal product for medical use. Bionorica. Kanefron N. Reg. No P N014244 / 01).

The disadvantage of this herbal preparation is possible dyspeptic disorders: nausea, vomiting, diarrhea, and the ethanol content limits the use of the drug for a number of diseases: alcoholism, liver disease, head injury, brain disease, peptic ulcer of the stomach and duodenum in the acute stage, childhood. In addition, during the treatment with the drug, the use of a large amount of liquid is recommended, which is contraindicated in case of impaired heart or kidney function and with high blood pressure.

The task is to offer a composition of medicinal plants, which will not have negative effects, as in the prototype, and will expand the base of used medicinal plants.

The technical result is a pronounced nephroprotective effect in the treatment of glomerulonephritis.

The technical result is achieved by the fact that a composition with a pronounced nephroprotective effect is made up of medicinal plants growing in the Far East under natural conditions in the following proportions in wt.%:

mountaineer bird grass 20-25 lingonberry leaves 13-17 viburnum sargent fruits 5-9 rose hips different Far Eastern fruit species 20-25 Schisandra chinensis seeds 13-17 wild strawberry leaf 5-9 corn silk 5-9

The studies were performed on male rats of the BD-9 Dark Agouty line weighing 160-180 g. The specific activity of the nephroprotective composition was studied on the model of experimental Heimann glomerulonephritis.

Studies have shown an improvement in renal depuration function, restoration of urine output, decreased erythrocyturia, a shift in urine reaction towards an increase in pH, a decrease in swelling of the stroma, capillary loop, a decrease in tubular dystrophy and leukocyte infiltration. A certain dose dependence of the nephroprotective activity of the composition was established and good tolerance was revealed. The composition was most active in the form of infusion at a dose of 20 ml / kg on day 28.

Based on the analysis of the obtained data, it was found that the effect of the nephroprotective composition is realized through a decrease in the severity of autoimmune processes in the glomeruli of the kidneys of Table 1, namely, the nephroprotective composition reduced immunological and biochemical parameters compared with the control: the level of circulating immune complexes, cryoglobulins, creatinine, urea. Proteinemia also decreased significantly, which indicated an improvement in renal depuration function when using this collection. The nephroprotective composition also acts through the normalization of urodynamics Table 2.

Thus, the nephroprotective composition had a positive effect on the main indicators of glomerulonephritis: it helped restore diuresis, reduce proteinuria, reduce azotemia, cryoglobulinemia and the level of circulating immune complexes. In general, the studied composition was not inferior in terms of nephroprotective activity to the Kanefron prototype, and in a number of indicators it exceeded it.

The advantages of the proposed composition:

- does not cause dyspeptic reactions from the gastrointestinal tract;

- does not require the use of a large amount of liquid;

- it is possible to use in a wider circle of patients, including children;

- plants do not require special cultivation;

- the plants are more active, as they grow under natural conditions, namely, lemongrass Chinese, rose hips, sparkling, wrinkled, Maksimovich, Ussuri, wild strawberries, viburnum Sargenta are endemic to the Far East;

- the composition has a pleasant smell and taste;

- it is possible to use in the treatment of any non-infectious chronic inflammatory diseases of the kidneys;

- does not contain ethyl alcohol.

Table 1 The effect of nephroprotective composition on biochemical and immunological parameters of blood with glomerulonephritis Group / drug CEC,% KG OD × 100 Creatinine, μM / L Urea, mmol / l Total protein, g / l 11th knots Intact group 10-14 0.6-1.4 49-59 4.1-4.5 68-80 rats Group con 28-36 * 3.9-4.9 * 98-114 * 7.3-7.7 * 51-59 * Rats Capefron N, 3.0 ml / kg 25-33 * 2.6-3.8 * 85-99 * 6.2-6.6 * 53-59 * NK, 5.0 ml / kg 24-36 * 2.8-3.4 * 87-105 * 6.5-7.3 * 47-65 * NK, 20.0 ml / kg 24-30 * 3.8-4.2 * 87-101 * 6.4-7.0 * 53-61 * 28th day Intact group 5-15 0.4-1.4 57-65 3.4-4.4 71-79 rats Group con 43-51 * 5.5-6.7 * 167-181 * 8.7-9.1 * 29-35 * trol rats Capefrop N, 3.0 ml / kg 16-28 * / ** 2.7-3.1 * 7 ** 99-111 * / ** 4.7-5.3 ** 61-71 ** NK, 5.0 ml / kg 28-38 * / ** 5.5-6.1 * 111-121 * / ** 5.7-6.1 ** 47-55 ** HK, 20.0 ml / kg 14-26 * / ** 1.7-2.7 * / ** 75-91 * / ** 5.3-5.9 ** 63-69 **

where the CEC - circulating immune complexes,

KG - cryoglobulins,

NK - nephroprotective composition,

* - differences are significant but compared with intact ones, p <0.05,

** - differences are significant compared with the control, p <0.05.

table 2 The effect of infusion nephroprotective composition on the excretory function of the kidneys with glomerulonephritis Group / drug Diuresis,% 01 load / 4 hours Protein, g / l Red blood cells, cells / μl PCE G / mg protein Group con 88-98 0.25-0.35 0-5 0.09-0.17 trol rats Group con 26-38 * 2.41-3.09 * 370-470 * 2.06-2.28 * trol rats Kanefron N, 3.0 ml / kg 69-77 * / ** 0.52-0.72 * / ** 12-38 * / ** 0.93-1.25 * / ** NK 5.0 ml / kg 47-61 * / ** 1,52-l, 77 * / ** 47-53 * / ** 2.08-2.28 * NK 20.0 ml / kg 64-74 * / ** 0.72-0.84 * / ** 15-35 * / ** 1.56-1.74 * / **

where PCE is pseudocholinesterase,

NK - nephroprotective composition,

* - the signs are significant in comparison with the iptacts, p <0.05,

** - differences are significant compared with the control, p <0.05.

Claims (1)

The use of a composition containing highlander bird grass 20-25 wt.%, Lingonberry leaves 13-17 wt.%, Viburnum Sargent fruits 5-9 wt.%, Rose hips of different Far Eastern species fruits 20-25 wt.%, Schisandra chinensis seeds 13- 17 wt.%, Wild strawberries leaves 5-9 wt.%, Corn stigmas 5-9 wt.%, Representing an infusion of the listed raw materials as a means with nephroprotective activity in the treatment of glomerulonephritis.