RU2553367C1 - Disinfectant for sanitation of veterinary supervision facilities - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: disinfectant for sanitation comprises colloidal silver stabilized with didecyldimethylammonium bromide, alkyl polyglucoside, alkyl-fraction C₄-C₁₀ and water in the following ratio of components, wt %: colloidal silver 0.004-0.04, didecyldimethylammonium bromide 0.4-0.8, alkyl polyglucoside, alkyl-fraction C₄-C₁₀ 0.3-0.6, water - the rest.

EFFECT: use of the invention enables to increase the biocidal activity while reduction of toxicity.

2 cl, 9 tbl, 4 ex

Description

FIELD OF THE INVENTION

The invention relates to sanitation, in particular to a disinfectant for sanitation of objects of veterinary supervision, and can be used as a disinfectant for the sanitation of livestock buildings, food and processing industries.

For the production of disinfectants, substances from the following chemical groups are most often used: peroxides, halogens, alcohols, aldehydes and dialdehydes, guanidines, surfactants. In most cases, preference is given to combination preparations containing 2-3 compatible active substances from various groups of chemical compounds (Mashneva L.V. Disinfectants - what to choose? / L.V. Mashneva // Meat Technologies. - 2011. - No. 9. - S. 66-68).

Known disinfectant composition consisting of hydrogen peroxide, water-soluble sodium salts of ethylenediaminetetraacetic acid, surfactant, flavoring and solvent (patent RU No. 2360416, CL A01N 25/22, A01N 43/14, A01N 33/08, A01P 1/00, publ. 07/10/2009).

The disadvantage of this composition is that the complexing agent requires a single mechanical activation, as a result of which the solutions are not subject to subsequent dilution, which complicates their storage and use.

Known disinfectant detergent containing alkyldimethylbenzylammonium chloride, polyhexamethylene guanidine hydrochloride, or polymethylene guanidine hydrophosphate, or a mixture thereof and nonionic surfactant, high molecular weight polyoxyethylene with a molecular weight of 1 × 106 × 109 and / or high molecular weight polyacrylamide with a ratio of 106 × 10 × 10 and having a molecular weight of 106 × components, parts by weight:

Alkyldimethylbenzylammonium chloride 0.03-54.0 Polyhexamethylene guanidine hypochloride or polymethylene guanidine hydrophosphate or their mixture in any ratio 0.01-33.0 Nonionic Surfactant 0.01-30.0 High molecular weight polyoxyethylene and / or high molecular weight polyacrylamide 0.01-2.0 Water 45.0-160.0

Partially or completely, water can be used water containing silver ions obtained by electrolysis by dissolving a silver electrolyte, and / or water that was in contact with schungite, and / or water, to which silver nanoparticles are added (patent RU No. 2315626, CL A61L 2/16, C11D 3/48, publ. 01.27.2008).

The disadvantage of this disinfectant is that silver, which is part of the water for dilution, is taken without a certain concentration, which can lead to an increase in microbiological parameters in solutions containing the same amount of the main active substance.

The closest in technical effect is a disinfectant for the treatment of skin containing colloidal silver, at least one cationic surfactant, auxiliary additives and water in the following ratio, wt.%:

colloidal silver 10 ^-5 -0.5 cationic surfactant 0.01-25 water and auxiliary additives up to 100

As cationic surfactants can be used: benzyl dimethyl [3- (myristoylamino) propyl] ammonium chloride (HBDMAPA), alkyl dimethylbenzylammonium salts, dioctyldimethylammonium, didecyldimethylammonium, ethylmethylmethylmethyl-dimethylamyldimethylamide dimethylamide ethyl -benzyl) ammonium, dimethylbenzylammonium, alkyltrimethylammonium, e.g. chlorides, propionates, methyl sulfates, octenidine dihydrochloride, N, N-bis- (3-aminopropyl) dodecylamine, poly (hexamethylene guanidinium) chloride, phosphate , gluconate, monohydrate and other polyalkylene guanidines, poly (4,9-dioxa-dodecane-1,12-guanidinium) phosphate, polyhexamethylene biguanide salts - hydrochloride, gluconate and others, as well as other cationic surfactants.

The silver content in the disinfectant preparation according to the invention can be from 10 ^-5 wt.% To 0.1 wt.%, And in the concentrate of the disinfectant, which must be diluted to 0.5 wt.% Before use.

The cationic surfactant content or the total content of several cationic surfactants in the antibacterial preparation according to the invention is from 0.01 wt.% To 1 wt.%, And in the disinfectant concentrate, which must be diluted before use to 25 wt.% .

As additives, propylene glycol (a water-retaining and bactericidal agent), polyethylene glycol (thickener) and a food-grade acid salt (pH disinfectant regulator) selected from the group consisting of: sodium or potassium citric acid, as well as sodium or potassium salt of lactic acid can be used (RU patent No. 2427380, class А61К 33/38, А61К 31/14, A61L 2/16, А61Р 17/02, published on 08.27.2011).

The disadvantage of this disinfectant is the low foaming effect.

In common with the claimed agent are colloidal silver and a cationic surfactant. The difference from the prototype of the claimed disinfectant for sanitation of objects of veterinary surveillance is the presence of an additional non-ionic surfactant alkylpolyglucoside, which allows the formation of resistant foam and degreases the treated surfaces faster, which helps to increase the quality of disinfection.

Brief description of drawings and other materials

In the table. 1 given a disinfectant for sanitation of objects of veterinary surveillance, the determination of the sensitivity of the microorganism Escherichia coli to a disinfectant for sanitation of objects of veterinary surveillance.

In the table. 2 - also, the sensitivity of the culture of Escherichia coli to various concentrations of a disinfectant for the rehabilitation of objects of veterinary surveillance.

In the table. 3 - also, hematological parameters of white mice (n = 8).

In the table. 4 - also, leukocyte formula of white mice,% (n = 8).

In the table. 5 - also, the scheme of experience and the results of studying the acute toxicity of a disinfectant for the rehabilitation of objects of veterinary surveillance in white mice.

In the table. 6 - also, acute toxicity of a disinfectant for sanitation of objects of veterinary supervision with a single intragastric administration for white mice (mg / kg).

On chart 7 - also, a graphical representation of the toxicity of the disinfectant for sanitation of objects of veterinary supervision and calculation of LD ₁₆ (probit 4) and LD ₈₄ (probit 6) by probit analysis (according to A.A. Stupnikov, 1975).

In the table. 8 - also, the results of tests of a disinfectant for the rehabilitation of objects of veterinary supervision in a production environment.

In the table. 9 - also, the quality indicators of cow's milk after testing the disinfectant for sanitation of objects of veterinary supervision (n = 40).

Disclosure of invention

The objective of the invention is to develop a highly effective disinfectant that is environmentally friendly, does not adversely affect equipment, animals and people and has good cleaning properties. For this, a complex of didecyldimethylammonium bromide, alkyl polyglucoside and colloidal silver was used as an active active ingredient in a disinfectant.

The technical result that can be achieved using the present invention is to increase the biocidal action of the disinfectant, reduce toxicity due to the fact that the concentration of the active substance is reduced, and increase the washing properties due to the introduction of alkylpolyglucoside.

The technical result is achieved using a disinfectant for sanitation of objects of veterinary supervision, including colloidal silver stabilized with didecyldimethylammonium bromide, and water, characterized in that it additionally introduces alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions with vigorous stirring and processing of the reaction mass with external physical influences , namely thermal, ultrasonic and ultraviolet radiation, with the following ratio of components, wt.%:

Colloidal silver 0.004-0.04 Didecyldimethylammonium bromide 0.4-0.8 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.3-0.6 Water Rest

The inventive disinfectant for sanitation of objects of veterinary surveillance is an aqueous solution of yellow color, odorless, has a pronounced bactericidal effect, does not have a local irritating and sensitizing effect, which makes it possible to use it in the presence of animals; It has pronounced washing properties and does not cause damage to the treated surfaces.

The effectiveness of disinfectants is determined by many factors: composition, active substance content, production technology, microflora at the objects to be treated, disinfection method (Noskova A.V. New disinfectants / A.V. Noskova // Veterinary medicine. - 2009. - No. 9 . - S. 43-45.).

Quaternary ammonium compounds from the group of cationic surfactants have both a disinfectant and detergent effect. They can have a neutral pH (from 5.5 to 7.5), which does not require specialized protection of the skin and mucous membranes. Do not damage the surface. In addition to the high bactericidal activity of preparations containing quaternary ammonium compounds, there is no inhalation toxicity (Fadeeva L.L. Cationic surfactants as a biocidal basis of modern antiseptics / L.L. Fadeeva and [et al.] // Veterinary medicine. - 2004. - No. 5. - P. 41-43; Khudyakov A.A. Effective disinfection and selection of a disinfectant / A.A. Khudyakov // Veterinary Medicine. - 2010. - No. 2. - P. 18-22).

The bactericidal effect of cationic surfactants is associated with inactivation of the synthesis of cell enzymes, denaturation of cell proteins and impaired permeability of cell membranes (Fomina T.A. Sanitary processing as a guarantee of high-quality products / T.A. Fomina, M.Yu. Minaev // Meat technologies . - 2012. - No. 1. - S. 42-43).

Alkylpolyglucoside is a nonionic surfactant, forms a stable foam and has good detergent properties. In addition, alkylpolyglucoside is biodegradable, since it is obtained from plant materials, therefore, it does not harm animals, staff and the environment.

Silver-based preparations provide an antibacterial effect on a wide range of bacteria, destroying the bacterial cell membranes and inhibiting their growth. Colloidal silver kills up to 99.9% of bacteria and prevents the appearance of odors (Smirnov AM Veterinary and sanitary aspects of the use of nanosilver / AM Smirnov and [et al.] // Vetkorm. - 2011. - No. 3. - P. 18-19) .

The mechanism of action of this agent is that the cationic surfactant is adsorbed on the cell membrane, and colloidal silver penetrates the nucleus, this leads to lysis of the cell membrane, impaired osmotic metabolism, protein precipitation, destruction of the genetic information of DNA, and as a result of the death of bacteria, in this case, the action of alkyl polyglucoside provides the formation of resistant foam, which helps to maintain the concentration of the active substance on the treated surface.

The essence of obtaining a disinfectant for sanitation of objects of veterinary surveillance is as follows: a solution of silver nitrate in the presence of didecyldimethylammonium bromide and alkyl polyglucoside is added to an aqueous solution of sodium borohydride with vigorous stirring and processing of the reaction mass with external physical influences, namely thermal, ultrasonic and ultraviolet radiation. Before use, the drug is diluted 100-1000 times.

The implementation of the invention

Examples of specific performance of obtaining and testing a disinfectant for the rehabilitation of objects of veterinary surveillance.

Example 1

A disinfectant for the rehabilitation of objects of veterinary supervision is obtained as follows. 1.6 ml of a 6% solution of didecidimethylammonium bromide and 2.2 ml of a 4.5% solution of alkyl polyglucoside are introduced into 96 ml of water, and the amount of sodium borohydride (0.02 ml of a 25% solution) that is stoichiometrically necessary to restore silver nitrate is dissolved. Then, with vigorous stirring and processing of the reaction mass with external physical influences, namely, thermal, ultrasonic and ultraviolet radiation, 0.02 ml of a 0.22% silver nitrate solution is dropwise added to the reaction mixture. Stirring is continued until the reaction is complete. The result is the following ratio of components, wt.%:

Colloidal silver 0,0004 Didecyldimethylammonium bromide 0.1 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.1 Water Rest

This tool, when testing the antimicrobial effect of the suspension method on cultures of Escherichia coli (strain 1257) and Staphylococcus aureus (strain 906), shows that the bactericidal activity against gram-positive bacteria is 87% and against gram-negative bacteria - 85%, starting from 5 min exposure.

Tests in production conditions give the following results: when controlling the quality of disinfection with E. coli and staphylococci, the disinfection efficiency of naturally contaminated objects is 81%, with an exposure of 30 minutes at a concentration of 0.25%.

Example 2

Carried out analogously to example 1, but take the following amount of water: 86 ml, a solution of didecidimethylammonium bromide - 6.7 ml, an alkyl polyglucoside solution - 6.7 ml, a sodium borohydride solution - 0.2 ml and 0.2 ml of silver nitrate solution the following ratio of components, wt.%:

Colloidal silver 0.004 Didecyldimethylammonium bromide 0.4 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.3 Water Rest

The disinfectant for sanitation of objects of veterinary supervision according to example 2 shows that its effectiveness against bacteria Escherichia coli (strain 1257) and Staphylococcus aureus (strain 906) is increased in comparison with example 1 and is 100%, starting from 5 min exposure.

The efficiency of disinfection of naturally contaminated objects under industrial conditions when monitoring the quality of disinfection of E. coli and staphylococci is 100% with an exposure of 30 minutes at a concentration of 0.25%.

Example 3

Carried out analogously to examples 1 and 2, but take the following amount of substances: water - 70 ml, a solution of didecidimethylammonium bromide - 13.4 ml, an alkyl polyglucoside solution - 13.4 ml, sodium borohydride solution - 2.0 ml and 2.0 ml of nitrate solution silver in the following ratio, wt.%:

Colloidal silver 0.04 Didecyldimethylammonium bromide 0.8 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.6 Water Rest

The obtained disinfectant according to example 3 has a pronounced (100%) bactericidal activity against gram-positive and gram-negative bacteria (Escherichia coli (strain 1257) and Staphylococcus aureus (strain 906)) when evaluated by the suspension method, starting from 5 min of exposure.

When testing the product under production conditions, the disinfection efficiency of naturally contaminated objects is 100% when controlling the quality of disinfection with E. coli and staphylococci, with an exposure of 30 minutes at a concentration of 0.25%.

Example 4

Carried out analogously to example 1, but take the following amount of substances: water - 20.0 ml, a solution of didecidimethylammonium bromide - 20.0 ml, an alkyl polyglucoside solution - 20.0 ml, a sodium borohydride solution -20.0 ml and 20.0 ml of nitrate solution silver in the following ratio, wt.%:

Colloidal silver 0.4 Didecyldimethylammonium bromide 1,2 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.9 Water Rest

The disinfectant of example 4 shows that its bactericidal activity against Escherichia coli and staphylococci decreased by 6% compared to example 3 and is 94% when evaluated by the suspension method, starting from 5 min of exposure, which is associated with the content of high concentrations of active substances in a disinfectant, leading to a decrease in the synergistic action of its components and a decrease in bactericidal effectiveness.

When disinfecting naturally contaminated objects under production conditions, the processing efficiency is 92% at a concentration of 0.25% with an exposure of 30 minutes.

Thus, the most optimal are examples 2, 3.

The disinfectant for sanitation of objects of veterinary surveillance was tested in laboratory conditions on pathogenic microorganisms and laboratory animals and in production conditions with the following ratio of components, wt.%:

Colloidal silver 0,022 Didecyldimethylammonium bromide 0.6 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.45 Water Rest

When determining the sensitivity to the studied preparation for the culture of Escherichia coli strain 1257, meat-peptone broth (MPB) is used as a nutrient medium. The broth culture is pre-diluted with saline to a cell concentration of 5 · 10 ⁶ / ml The scheme of the experiment is presented in table. one.

The contents of the tubes are mixed by shaking and incubated in a thermostat at a temperature of 37 ° C for 24 hours. At the end of the indicated time, the first three tubes are plated on nutrient medium in Petri dishes and placed in a thermostat at 37 ° C for 24 hours.

After a day, the changes that occurred in the tubes are recorded: in the first tube, the broth remains transparent, in the second tube the BCH slightly turbid, in the remaining tubes the broth becomes stable turbid. Then do the seeding of the first three tubes on a medium of MPB in a Petri dish of 0.1 ml and put in a thermostat at a temperature of 37 ° C for 24 hours.

After this time, the reaction of Escherichia coli microorganisms to various concentrations of a disinfectant for sanitation of objects of veterinary surveillance is observed. The results of the studies are presented in table. 2.

As can be seen from the table. 2, when the concentration of colloidal silver is 4.4 · 10 ^-5 mol / l, there is no growth of microorganisms in the nutrient medium; at a concentration of colloidal silver 4.4 · 10 ^-6 mol / l - there is a slight increase in the microorganisms Escherichia coli; at a concentration of 4.4 · 10 ^-7 mol / l, intensive growth of bacterial colonies is observed.

Thus, the minimum inhibitory concentration of a disinfectant for sanitation of objects of veterinary supervision is 4.4 · 10 ^-5 mol / l.

The experiment on finding the maximum tolerated dose (MTD) is carried out on clinically healthy white mice weighing 20-22 g. The starting dose for finding the minimally toxic doses (maximum tolerated) is 100 mg / kg body weight and in group 1, in which it was used, not changes in the behavior and physiological state of mice occur. In groups 2–8, in which doses of 200, 300, 400, 500, 600, 700, and 800 mg / kg, respectively, were introduced, no visible deviations were noted either. The dose of 900 mg / kg, administered to mice of the 9th group, although it does not cause death in any of the eight animals, but after the administration is observed: rapid breathing and palpitations, aggressive behavior, which are replaced by periods of deep oppression, and the state of oppression lasts 1.5 -2 hours. Then all the mice in the group return to normal and take food and water. Changes in hematological parameters are noted (Tables 3, 4).

In the analysis of hematological parameters, it was found that the number of red blood cells in groups No. 1-7 and No. 10 is within normal limits, and in groups No. 8-9 it decreases below normal and is 17.98 and 29.94% respectively less relative to control.

The hemoglobin level is reduced in groups No. 6-9 by 21.15%, 23%, 26.59 and 31.23% of the control group.

There is an increase in the number of leukocytes in groups No. 7-9, which is 16.07%, 31.35 and 36.05%, respectively, less than the control.

The data of the leukocyte formula and the number of platelets are in all experimental and control groups within the physiological norm.

Since when testing a dose of 900 mg / kg, phenomena and changes in hematological parameters are recorded, indicating poisoning of laboratory animals, but death is not observed, this dose is taken as the maximum tolerated (MTD) and the starting one for the experiment to determine lethal doses (table. 5).

To determine lethal doses of the drug, clinically healthy white mice weighing 20-22 g are used; studies are carried out according to the “Methodological Instructions for Assessing the Toxicity and Danger of Disinfectants” (MU 1.2.1105-02. - M., 2002.). The route of administration is chosen intragastric, because this product is intended for the processing of agricultural premises, equipment, feeders and drinking bowls, from which animals take food and water. With intragastric administration of the drug in the maximum possible amounts of death of animals is not observed, the results of the study on acute toxicity with the enteric route of administration are presented in table. 6 and graph 7.

The object of the study under production conditions is a typical barn in the SEC "Novomaryevsky" Shpakovsky district. A new tool is tested in 0.025-0.5% concentrations. The concentrated solution is diluted with tap water. Disinfection is carried out by small-droplet irrigation with predetermined solution concentrations after preliminary mechanical cleaning of the treated surfaces.

The quality of disinfection is controlled by the release of bacteria from the group of Escherichia coli and staphylococci from washes from naturally contaminated surfaces of the room. The tests are carried out in accordance with the "Rules for the disinfection and disinfestation of objects of state veterinary surveillance", M., 2002 and the "Recommendations for sanitary and bacteriological examination of flushes from the surface of objects subject to veterinary surveillance", M., 1988. Wash is the control from surfaces taken before disinfection.

Processing with a disinfectant for sanitation of objects of veterinary supervision is carried out in the absence of animals according to the scheme specified in table. 8.

When testing a 0.25% concentration of a new disinfectant for sanitizing veterinary surveillance objects after an exposure of 10 min, E. coli does not stand out on the treated objects, but staphylococci are noted on the floor, walls and partitions.

A test of 0.5% concentration with an exposure of 10 min shows that disinfection is achieved only with respect to E. coli.

Complete disinfection at all objects in the calf is achieved by exposure to solutions of 0.25 and 0.5% concentrations for 30 and 60 min with a single irrigation while controlling the quality of disinfection for the isolation of Escherichia coli and staphylococci.

Thus, the results of the experiments indicate that the new disinfectant for sanitation of objects of veterinary supervision has a pronounced bactericidal activity in 0.25% concentration (according to the drug) with an exposure of 30 minutes.

Take into account the quality indicators of milk of cows after testing the disinfectant for the rehabilitation of objects of veterinary supervision in a production environment (table. 9).

The amount of dry skim milk residue increases by 1.46% after 24 hours and by 2.47% after 5 days after processing to the data of samples taken before disinfection of the barn.

The fat content in samples of milk obtained 24 hours and 5 days after treatment increases by 1.68 and 4.84%, respectively.

There is an increase in protein in milk relative to the data obtained before the rehabilitation of the premises, by 1.25 and 11.2% after 1 and 5 days.

The density of milk rises by 1 unit 5 days after disinfection and is 1031 kg / m ³ , before processing and one day after processing it is 1030 kg / m ³ .

The acidity after processing changes by no more than 10% both in the direction of increase and decrease, and amounts to 17.7 and 17.1 ° T.

The number of somatic cells after disinfection of the premises is reduced by 5.67 and 5.9% after 24 hours and 5 days relative to the number of somatic cells in milk before processing the barn.

The data of all samples taken before and after processing are within normal limits and milk corresponds to GOST R 52054-2003.

The advantages of the proposed disinfectant for sanitation of objects of veterinary surveillance in that it contains a complex disinfectant of natural origin, cationic and nonionic surfactants with powerful biocidal action. At the same time, silver as a part of a disinfectant for sanitation of objects of veterinary supervision is in a nanoscale state. An increase in disinfecting ability is achieved with the synergistic action of the components, which is due to a decrease in the concentration of the active substance. As a result, the proposed disinfectant for the rehabilitation of objects of veterinary surveillance has pronounced properties to suppress pathogenic microflora and a low toxic effect for warm-blooded animals. Alkylpolyglucoside promotes the formation of a stable foam, which, when processing disinfectable surfaces, provides longer contact of the active substances with pathogenic microflora, which increases the quality of disinfection.

The invention in comparison with the prototype and other technical solutions has the following technical advantages:

- increased biocidal action;

- reduction of toxicity to warm-blooded;

- persistent foaming;

- ease of use;

- reduction of economic costs;

- lack of corrosive action.

Claims (2)

1. A disinfectant for sanitation of objects of veterinary supervision, including colloidal silver, stabilized with didecyldimethylammonium bromide, and water, characterized in that it additionally introduces alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions in the following ratio, wt.%:
Colloidal silver 0.004-0.04 Didecyldimethylammonium bromide 0.4-0.8 Alkyl polyglucoside, C ₄ -C ₁₀ alkyl fractions 0.3-0.6 Water Rest 2. A disinfectant for sanitation of objects of veterinary supervision according to claim 1, characterized in that it is diluted 100-1000 times before use.

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