RU2436593C2 - Biocide composition based on quaternary ammonium and method for preparing it - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, veterinary science, food industry, domestic service and concerns biocide disinfectants and sterilising agents. The biocide composition contains a mixture of three quaternary ammonium compounds in total contents 19-21 %, in the form of a mixture of 50 % water solution of didecyldimethylammonium chloride containing 20 % 2-propanol, 50 % water solution of cocobenzyldimethylammonium chloride containing 2 % ethylene glycol, 30 % water solution of N,N-bis(3-aminopropyl)-dodecylamine, and water and ethanol and/or isopropanol as a solvent. Also, what is disclosed is a method for preparing the biocide composition.

EFFECT: composition is more effective and can be used for disinfection and sterilisation of the various technological items.

3 cl, 7 tbl, 8 ex

Description

The invention relates to the field of medicine, veterinary medicine, the food industry, the household sector and relates to biocidal agents for disinfection and sterilization of objects.

The sanitary and hygienic conditions of life and work of a large number of people in conditions of increasing environmental pollution, microbiological safety (in medical institutions, pharmaceutical and food industries) can be significantly improved by using effective biocidal agents for disinfection and sterilization.

Currently, more than 70% of disinfectants registered in Russia and used in domestic medical practice are based on quaternary ammonium compounds (HOURs). The wide distribution of such drugs is associated with their low toxicity. To enhance the bactericidal effect of the compounds based on QAS, additional active substances are additionally introduced into them.

At the same time, when creating new biocidal agents, not only increasing their antimicrobial activity, but also increasing the duration of their antimicrobial activity after surface treatment, reducing toxicity, allergenicity and environmental safety are relevant.

A disinfectant exhibiting a biocidal effect is known, which contains a polyguanidine compound and a quaternary ammonium compound (HOUR) in a mass ratio of 1: 1 ÷ 4: 1, N, N-bis (3-aminopropyl) dodecylamine and water in the following ratio of components in wt. %: polyguanidine compound 1.5-2.0; Quaternary ammonium compound 0.1-1.5; N, N-bis (3-aminopropyl) dodecylamine 0.2-1.0; water - the rest (RF patent 2317950, publ. 17.07.2006). This tool has a fairly narrow scope - for the treatment of natural and wastewater.

Another example of the known biocidal disinfectants can serve agent containing a disinfecting agent comprising benzalkonium chloride with polyhexamethylene guanidine hydrochloride and / or poligeksametilenguanidinfosfatom, nonionic surfactant, water and the high molecular weight polyoxyethylene to 1 × ¹⁰ June ¹⁰ Sept. -1 × and / or high molecular weight polyacrylamide with a molecular weight of 1 × 10 ⁶ -20 × 10 ⁶ (RF patent 2315626, publ. 01.06.2006). The tool has a low toxicity, but a higher cost due to the inclusion of guanidine compounds.

A disinfectant is also known that includes, as an active ingredient, a quaternary ammonium compound and / or a derivative of polyhexamethylene guanidine and / or N, N-bis (3-aminopropyl) dodecylamine, a nonionic surfactant, and salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acid, as well as a dye and water, with a certain ratio of components, wt.%. (RF patent 2297248, publ. 27.10.2005). The tool is not effectively used to sterilize medical equipment.

A disinfectant is known that contains alkyldimethylbenzylammonium chloride, N, N-bis (3-aminopropyl) dodecylamine, a nonionic surfactant, trilon B, isopropyl alcohol, polyvinylpyrrolidone and water in the following ratio, wt.%: Alkyldimethylbenzylammonium chloride -15.0; N, N-bis (3-aminopropyl) dodecylamine 5.5-8.0; nonionic surfactant 2.0-3.5; isopropyl alcohol 9.0-15.0; polyvinylpyrrolidone 1.0-3.0; Trilon B 0.2-0.5; water up to 100.0. The disinfectant may additionally contain an alkaline agent in a concentration of 0.2-1.40%, which is used as an alkali metal salt with organic and / or inorganic acids, mainly carbonic, acetic, phosphoric, benzoic. Solving the task of creating a disinfectant with low toxicity, this tool does not contain chlorine and aldehydes. The tool has a high activity against tuberculosis and polio virus. The disinfectant has good wetting and washing properties and at the same time has low toxicity (RF patent 2308292, publ. 22.03.2005). This solution can be indicated as the closest analogue - the prototype.

The present invention is the development of a new effective biocidal agent.

The problem is solved by the proposed biocidal composition based on quaternary ammonium compounds and the method for its preparation.

The new biocidal composition is a versatile, highly effective and economical disinfectant.

The composition contains as an active substance a mixture of three quaternary ammonium compounds at a total content of 19-21%, in the form of a mixture of a 50% aqueous solution of didecyldimethylammonium chloride containing 20% 2-propanol, a 50% aqueous solution of cocobenzyl dimethylammonium chloride containing 2% ethylene glycol, 30 % aqueous solution of N, N-bis (3-aminopropyl) dodecylamine, water and ethanol and / or isopropanol are used as a solvent in the following components, wt.%:

50% didecyldimethylammonium chloride solution 5-25 50% solution of cocobenzyl dimethylammonium chloride 5-25 30% solution of N, N-bis (3-aminopropyl) dodecylamine 5-15 Ethanol and / or Isopropanol 18-22 Water rest,

at a pH of the composition 5.5-6.

The composition of the biocidal composition is a concentrate for the production of disinfectants and sterilizing agents. It includes 21% of the active principle and is selected in such a way that, with a given ratio of three quaternary ammonium compounds, it provides high efficiency both at certain dilutions and in undiluted form, depending on the objectives of the invention.

Didecyldimethylammonium chloride, 50% aqueous solution containing 20% 2-propanol - bactericide, fungicide, algicide.

Cocobenzyl dimethylammonium chloride, 50% aqueous solution containing 2% ethylene glycol - biocide, bactericide, fungicide, algicide.

N, N-bis (3-aminopropyl) dodecylamine, 30% aqueous solution -

bactericide, virucide, fungicide, tuberculocide.

In accordance with the purposes of use, the composition may further comprise technological or functional additives, such as emulsifiers, stabilizers, blowing agents, perfumes, colorants, rheology modifiers, antioxidants, peroxide compounds, and the like.

Another object of the invention is a method for producing a biocidal composition. The proposed method includes the following stages: dissolve didecyldimethyl ammonium chloride in 2-propanol and add water to obtain a 50% solution, dissolve cocobenzyl dimethyl ammonium chloride in water, add ethylene glycol and water to obtain a 50% solution, dissolve N, N-bis (3 α-aminopropyl) -dedecylamine in water to obtain a 30% solution, the resulting solutions are mixed in predetermined proportions, then ethanol and / or isopropanol are added to the resulting mixture, and at the same time, water with vigorous stirring using high speed a propeller stirrer at a speed of 500 to 1000 rev / min for 3-5 minutes, if necessary, is adjusted with water the total composition to 100% and adjusted to pH 5.5-6.

If it is necessary to introduce technological or functional additives, they are introduced into a mixture of solutions of quaternary ammonium compounds, dissolving in the corresponding aqueous or organic phases, depending on their solubility.

To adjust the pH, any known means can be used - sodium or potassium hydroxides, amines, hydrochloric acid, phosphoric acid, phosphate buffer, citrate, etc.

The method may include an additional processing step for, for example, 3-5 minutes with ultrasound, preferably with a frequency of 15-17 kHz.

The use of intensive mixing with the simultaneous introduction of streams of alcohol and water allows you to get a highly stable system, to reduce the content of harmful impurities generated in the process of heat generation that occurs when water and alcohol are mixed. Since when mixing ethyl alcohol with water, a decrease in the volume of the mixture is observed - adiabatic compression (contraction), the value of which depends on the strength of the alcohol, it may be necessary to add water to obtain 100 wt.% The final composition.

The resulting biocidal composition is low toxic and environmentally friendly, does not contain chlorine and aldehydes.

It has a wide range of antimicrobial activity in relation to:

- gram-positive and gram-negative bacteria (including pathogens of nosocomial infections, tuberculosis);

- viruses (in relation to causative agents of acute respiratory viral infections, parenteral hepatitis, poliomyelitis, HIV infection);

- pathogenic fungi (including mold fungi, causative agents of candidiasis, trichophytosis - Trichophyton gypseum and dermatophytosis).

The composition has detergent, deodorizing, anticorrosive properties, does not spoil any processed objects and does not discolor fabrics.

The advantage of the composition is that it does not have a fixing effect. In addition, it is characterized by a prolonged residual antimicrobial effect.

The new composition does not have sensitizing and cumulative properties; there are no long-term manifestations (mutagenic, embryotoxic, teratogenic, carcinogenic). It can be used in the presence of people.

In particular, the following fields of application of the claimed composition are possible:

for prophylactic, current and final disinfection of surfaces in rooms, including soft (floor coverings, upholstery fabrics, upholstered furniture), instruments, equipment, including couves for premature babies;

for disinfection of medical devices (including surgical and dental instruments, including rotary and locking, saliva ejectors, denture blanks made of metal, ceramics, plastics, articulators);

for disinfection of patient care items from various materials, toys;

linen (underwear, bedding, overalls for staff, etc.); shoes made of various materials;

for disinfection of tableware, pharmacy and laboratory glassware;

for disinfection of sanitary equipment; cleaning material, rubber mats;

for disinfection of medical waste, including disposable products before disposal;

for disinfecting blood before its disposal;

for pre-sterilization cleaning of medical devices, including surgical and dental instruments, combined and not combined with disinfection, manual and mechanized (using RCD) methods;

for preliminary and sterilization (final) cleaning of rigid and flexible endoscopes and instruments for them manually and mechanically;

for carrying out general cleanups in medical and preventive, penitentiary and child care facilities;

for disinfection in catering and trade:

canteens, cafes, bars, restaurants, consumer markets, supermarkets, etc .;

for disinfection of household facilities (hotels, hostels, sanitary inspection rooms, baths, saunas, laundries, hairdressers, public toilets, swimming pools, sports and recreation centers, cinemas, offices, etc.):

for disinfection of surfaces from any materials in the premises (floor, walls, hard furniture), including soft (carpet and other floor coverings, upholstery fabrics, upholstered furniture);

for disinfection of tools (cosmetic and hairdressing salons), care items and personal care products, special containers and trays, etc .;

for disinfection of residual amounts of fecal-urinary mixture in storage tanks of autonomous toilets;

for disinfection and deodorization of garbage equipment, garbage trucks and garbage bins;

for processing rooms for keeping animals, as well as various surfaces and equipment of diversified livestock complexes, poultry farms, etc.

Effect: expanding the range of applications, including in cases of resistant strains of microorganisms.

The invention may be illustrated, but not limited to the following embodiments.

Example 1

The composition of the biocidal composition with a total content of the active principle of 19%:

50% aqueous solution of didecyldimethylammonium chloride containing 20% 2-propanol 5% 50% aqueous solution containing 2% ethylene glycol 24% Cocobenzyl Dimethyl Ammonium Chloride 30% aqueous solution of N, N-bis (3-aminopropyl) dodecylamine fifteen% Ethanol 22% Citrus flavoring (TU 64-19-149-92) 0.5% Water rest

Solutions of quaternary ammonium compounds are prepared separately: 50 g of didecyldimethylammonium chloride are dissolved in 20 g of 2-propanol and water is added to obtain a solution with a total weight of 100 g, 50 g of cocobenzyl dimethylammonium chloride are dissolved in water, 2 g of ethylene glycol are added and water is added to obtain a solution with a total weighing 100 g, 30 g of N, N-bis (3-aminopropyl) dodecylamine are dissolved in approximately equal amounts of water and the mass of the solution is adjusted with water to obtain a solution with a total mass of 100 g. The resulting solutions are mixed in predetermined proportions. Add fragrance in an amount of 0.5 g. Then, 22 g of ethanol and 33.5 g of water are added to the resulting mixture with vigorous stirring using a high-speed propeller stirrer at a speed of 1000 rpm for 2 minutes, then the total weight of the composition is adjusted with water to 100% and adjust the pH to 5.5-6 with citrate buffer.

Example 2

The composition of the biocidal composition with a total content of the active principle of 21%:

50% aqueous solution of didecyldimethylammonium chloride, containing 20% 2-propanol twenty% 50% aqueous solution of cocobenzyl dimethylammonium chloride, containing 2% ethylene glycol 19% 30% aqueous solution of N, N-bis (3-aminopropyl) dodecylamine 5% Isopropanol twenty% Water rest

Example 3

The composition of the biocidal composition with a total content of the active principle of 21%:

50% aqueous solution of didecyldimethylammonium chloride containing 20% 2-propanol fifteen% 50% aqueous solution of cocobenzyl dimethyl ammonium chloride containing 2% ethylene glycol 21% 30% aqueous solution of N, N-bis (3-aminopropyl) dodecylamine 10% Ethanol and Isopropanol 1: 2 twenty Acid Triphenylmethane Green (TU 6-14-390-80) 0.1 Water rest,

Example 4

The study of the disinfectant properties of the composition

A laboratory experimental study was carried out on 4 reference strains of test microorganisms, regulated by the "Methods of testing disinfectants to assess their safety and effectiveness", approved. Ministry of Health of the Russian Federation, 1998 - Staphylococcus aureus 906, Escherichia coli 1257, Pseudomonas aeruginosa (nosocomial strain - 1544); C. albicans 15, Mycobacterium B ₅ .

A sample of the biocidal composition of example 1 was sequentially diluted to a final concentration of 0.025% -2.5% (for the active substance). The solvent used is drinking water. The exposure time ranged from 15 minutes to 120 minutes. Twice processing of test objects was carried out with an interval of 15 min.

During the experiments, a universal neutralizer was used to neutralize the active substance: tween-80 - 3%, saponin - 3%, histidine - 0.1%, cysteine - 0.1%.

The criterion for the effectiveness of the drug is 100%.

In accordance with the data on the study of the effectiveness of the composition in the disinfection of dishes, linen and patient care items contaminated with bacteria, the composition is effective in concentrations of 0.025-0.1%.

So, a 0.025% solution of the composition provides disinfection of dishes without food debris contaminated with S.aureus, E.coli and Ps. aeruginosa after 30 minutes, and 0.05% after 15 minutes.

Patient care items made of rubbers, plastics, glass, metals contaminated with S. aureus and E. coli, Ps. aeruginosa was disinfected with a solution of a concentration of 0.05% after 60 minutes. An increase in the concentration of the solution to 0.1% made it possible to effectively decontaminate test objects in 30 minutes. Toys made of various materials (plastic, metal, rubber) were disinfected by treatment with 0.1% solutions after 60 minutes.

The disinfecting activity of 0.05%, 0.1%, 0.25% solutions is presented in tables 1-3.

Table 1 The disinfecting activity of a 0.05% (active ingredient) solution in the disinfection of test surfaces contaminated with pathogens of bacterial etiology, including pathogens of nosocomial infections

Objects of disinfection Exposure (minutes) The effectiveness of disinfection in relation to (abs. Average,%) The method of disinfection S.aureus (1.9 × 10 ⁵ CFU / cm ² ) E. coli (1.78 × 10 ⁵ CFU / cm ² ) Ps. aeruginosa (1.8 × 10 ⁵ CFU / cm ² ) CFU / cm ² (M) % CFU / cm ² (M) % CFU / cm ² (M) % Test surfaces thirty 42.5 99.97 ± 0.01 33.3 99.98 ± 0.2 22.0 99.98 ± 0.2 Wiping 60 7.3 99,99 5.5 99.99 ± 0.1 1.7 99.99 ± 0.1 120 0 one hundred 0 one hundred 0 one hundred Test surfaces 120 * 0 one hundred 0 one hundred 0 one hundred Irrigation Note: (M) is the average number of colonies; * - the minimum time at which 100% efficiency is observed.

table 2 The disinfecting activity of a 0.1% (active substance) aqueous solution during the disinfection of test surfaces contaminated with pathogens of bacterial etiology, including pathogens of nosocomial infections Disinfection objects Exposure (minutes) The effectiveness of disinfection in relation to (abs. Average,%) The method of decontamination S. aureus (1.9 × 10 ⁵ CFU / cm ² ) E. coli (1.78 × 10 ⁵ CFU / cm ² ) Ps. aeruginosa (1.8 × 10 ⁵ CFU / cm ² ) CFU / cm ² (M) % CFU / cm ² (M) % CFU / cm ² (M) % Test surfaces thirty 14.2 99.97 ± 0.01 3.3 99.98 ± 0.2 22.0 99.98 ± 0.2 Wipe 60 0 99,99 0 99.99 ± 0.1 1.7 99.99 ± 0.1 Test surfaces 60 * 4.1 one hundred 0 one hundred 0 one hundred Irrigation Test surfaces 60 6.8 99,99 4,5 99,99 - - Brushing Note: (M) is the average number of colonies; * - the minimum time at which 100% efficiency is noted;
- no studies have been conducted.

Table 3 Disinfecting activity of 0.25% (according to the preparation) of an aqueous solution during the disinfection of test surfaces contaminated with pathogens of bacterial etiology (except for tuberculosis) Disinfection objects Exposure (minutes) The effectiveness of disinfection in relation to (abs. Average,%) The method of disinfection S.aureus (1.68 × 10 ⁵ CFU / cm ² ) E.coli (1.75 × 10 ⁵ CFU / cm ² ) CFU / cm ² (M) % CFU / cm ² (M) % Test surfaces fifteen 0 one hundred 0 one hundred Wipe Test surfaces fifteen 4.7 99,99 0 one hundred Irrigation

The study of the activity of aqueous solutions of the biocidal composition according to example 2 in relation to Mycobacterium B ₅ showed (table 4) that it is effective in concentrations of 0.5-0.75-1.0%.

Table 4 The disinfecting activity of an aqueous solution of mycobacteria (with Kef = 100%)

Test objects The concentration of the working solution of the drug (for the drug) (%) ' Disinfection Time (min) The method of disinfection Indoor surfaces, hard furniture, surfaces of appliances, equipment 0.5 60 Rubbing or irrigation 0.75 thirty The surfaces are soft, including carpet and other floor coverings, upholstery fabrics, upholstered furniture. 0.75 60 Brushing Dishes without food debris 0.25 60 Immersion 0.5 thirty Dishes with leftover food 0.75 60 Immersion 1,0 thirty Uncleaned laundry 0.5 60 Soak Healed laundry 0.75 60 Soak Patient care items, toys 0.5 60 Dipping or rubbing 0.75 thirty Medical waste 0.75 120 Immersion Sanitary equipment 0.75 60
90 Wiping
Irrigation Medical Products 0.5 60 Immersion 0.75 thirty Harvesting material 0.75 60 Immersion 1,0 thirty

As follows from the data presented, the active concentration during exposure for 60 minutes is from 0.25 to 0.75, while according to the patent of the Russian Federation 2308292 - 1%.

Example 5

The study of biocidal activity to resistant strains

Certain strains of test microorganisms are also characterized by their level of resistance to reference disinfecting agents. In connection with the increasing introduction into practice of medical disinfection of surfactant-based products, in particular HOUR (Quaternary ammonium compounds), the problem of the possible formation of bacterial resistance to them is becoming more and more urgent. To solve this problem, it is necessary to monitor the resistance of hospital and other bacterial strains to disinfectants, to develop a standard method for determining the resistance of bacteria to disinfectants.

Research in this area, conducted by the Research Institute of Disinfectology Rospotrebnadzor, Department of Disinfectology MMA named after I.M.Sechenova, showed that regarding quaternary ammonium compounds and tertiary amines, T.gypseum is the most stable microorganism, followed by Mycobacterium B5 and Candida albicans. Poliovirus in terms of resistance is on the same level with the latter or follows them.

Mold and yeast fungi can act as causative agents of nosocomial infections (nosocomial infections), causing severe pneumonia, generalized processes and skin lesions. Newborns often become infected with yeast-like fungi of the genus Candida, in some carriage of mushrooms is formed for many years. The external environment is a very important factor in the epidemiology of fungal diseases. Therefore, the establishment of high efficiency of the proposed new composition in relation to the most stable strain of T.gypseum, gives a definite advantage to the created biocidal agent.

Data on the fungicidal activity of the composition of example 3 when disinfecting various objects are presented in table 5.

The time indicated in the table is the time during which, at the indicated concentration, the efficiency coefficient is 100%.

Table 5 Test objects The concentration of the working solution (drug) (%) Disinfection time (minutes) The method of disinfection C, albicans T. gypseum Cladosporium spp., Mucor spp. Indoor surfaces, hard furniture, surfaces of appliances, equipment 0.25 thirty 60 60 Rubbing or irrigation The surfaces are soft, including carpet and other floor coverings, upholstery fabrics, upholstered furniture. 0.75 - 60 60 Brushing Dishes without food debris 0.1 60 - - Immersion 0.25 fifteen - - Dishes with leftover food 0.25 60 - - Immersion Non-contaminated patient care items, toys 0.2 45 60 60 Dipping or rubbing 0.25 thirty 45 45 Excreted laundry 0.25 60 90 90 Soak Medical waste 0.25 120 120 120 Immersion Sanitary equipment 0.25 60 90 90 Wiping
Irrigation 0.5 60 90 90 Test products for mecicin purposes 0.25
0.75 60 90
thirty 90 Immersion Harvesting material 0.25 60 90 90 Immersion Rubber mats 0.5 60 60 60 Dipping or rubbing 0.75

Example 6

The study of residual biocidal activity of solutions

In order to identify the residual effect of the agent for a long time (long-term), the test surfaces were treated with a working solution of the composition at concentrations effective against the used test microorganisms: E. coli and C. albicans and dried. Then, daily (for 3 days), the surfaces were contaminated with a suspension of test microorganisms (10 ⁵ CFU / cm ² ) and after 60 minutes of exposure the flush was taken. Controls were surfaces that were not treated with the solution of the test substance (see table 6).

Table 6 The study of long-term (within 4 days) residual antimicrobial action of 0.05% and 0.25% solutions of the drug Duration of observation (day) Microorganism test E. coli C. albicans Control (M) (CFU / cm ² ) Experience Control (M) (CFU / cm ² ) Experience (M) CFU / cm ² Efficiency ratio (%) (M) CFU / cm ² Efficiency coefficient ^) one 1.3 × 10 ⁴ 0 one hundred 1.2 × 10 ⁴ 0 100.00 2 0.9 × 10 ⁴ 105 98.83 0.8 × 10 ⁴ 146 98.17 3 1.1 × 10 ⁴ 1880 82.91 1.0 × 10 ⁴ 1278 87.22 four 0.8 × 10 ⁴ 6477 19.04 0.7 × 10 ⁴ 5800 17.14

The presence of residual antimicrobial action in aqueous solutions of the composition allows to reduce working concentrations with the regular use of the biocidal composition for surface treatment.

Example 8

Evaluation of the virucidal effect against hepatitis B virus

The studies were conducted according to the methods described in "Methods of testing disinfectants to assess their safety and effectiveness", M., 1998, part 1, 2, "Methodological recommendations for determining the virucidal activity of drugs No. 111973 from 09/06/73," for compliance requirements of the “Normative indicators of safety and effectiveness of disinfectants, subject to control during mandatory certification” No. 01-12 / 75-97.

All studies were carried out in 3 repeated experiments and subjected to statistical processing.

Efficiency evaluation criterion - not less than 100%.

When testing the composition used tests indirect determination of inactivation of the virus.

The characterized draining serum with a high protein load (52% in albumin due to the addition of human dry albumin to the serum) and a high content of viral particles was used as a substrate. When preparing the substrate, the following parameters were obtained:

1. HBsAg - positive reaction, concentration of 180 ng / ml.

2. HBsAg - a positive reaction.

3. The concentration of particles of hepatitis B virus is 2.5 · 10 ⁶ Geq / ml.

The initial parameters of the substrate for testing the biocidal composition were determined using diagnostic kits from Hoffman la Roche (Switzerland) on automatic analyzers CobasCore II for ELISA and CobasAmplicore (Switzerland) for PCR analysis. For the test, a substrate was used at a dilution of 1:10 (5.2% in albumin, which corresponds to the protein load of normal human blood serum) in physiological saline. For a model test for disinfection testing, 50 μl of the substrate in the dilutions to be tested was applied to the corresponding test object and dried for 60 min at room temperature. The biocidal composition was used in dilution from 0.25% to 1% by wiping, irrigation, immersion and soaking; exposure time - 30 min, 60 min and 90 min, temperature + 25 ° С. After surface treatment, the substrate was dried and the substrate was extracted with 1 ml of saline for 120 minutes. The resulting extract was analyzed for the presence of hepatitis B virus by the above methods.

The results of the study evaluating virucidal activity are presented in table 7.

Since the table shows the data for 100% effectiveness of the drug against the test virus, the data presented can be considered as recommended modes of use for disinfection of various objects with infections of viral etiology.

Table 7 Recommended modes of application of the biocidal composition in the processing of various objects for infections of viral etiology Disinfection object The concentration of the working solution (for the drug),% The time of disinfection, min Multiplicity of processing The method of disinfection Indoor surfaces (floors, walls, etc.), hard furniture, surfaces of apparatuses and devices 0.25 60 Once Rubbing, irrigation Patient Care Items from metals, plastics, glass 0.5 60 Once Dipping, rubbing rubber 0.5 60 Once Wiping Tableware no food residue 0.25 60 Once Immersion in excess solution with leftover food 1,0 60 Once The ware is pharmaceutical, laboratory; items for washing dishes 1,0 60 Once Immersion in excess solution Lingerie unpolluted 0.5 60 Once Immersion in excess solution polluted 1,0 90 Once Harvesting material 1,0 60 Once Immersion in excess solution Sanitary equipment 0.5 60 Once Rubbing, irrigation 1,0 thirty Medical Products from metals, plastics, glass 0.25 60 Once Dipping, rubbing 0.5 thirty rubber 1,0 60 Once Wiping dental instruments, including rotating 0.25 60 Once Immersion in excess solution 0.5 thirty 1,0 fifteen endoscopes and instruments for them 0.25 60 Once Immersion in excess solution 0.5 thirty 1,0 fifteen incubators 0.25 60 Once Immersion in excess solution 0.5 thirty Medical waste before disposal 1,0 60 Once Immersion in excess solution, wiping Toys 0.25 60 Once Rubbing, dipping, irrigation 0.5 thirty Rubber mats 1,0 60 Once Dipping, rubbing

The results obtained indicate the presence of virucidal activity of the claimed composition against hepatitis B virus in concentrations from 0.25% to 1.0% at various exposures depending on the tested objects.

All disinfectant solutions obtained in examples 1, 2 and 3, when exposed in the recommended modes, do not have a destructive effect on medical endoscopes of domestic and foreign production, their elements and tools for them and do not cause changes in their functional and physico-mechanical properties.

The new biocidal composition has high storage stability. The shelf life of the product is at least 5 years.

You can use the same solution repeatedly during the day.

Claims (3)

1. A biocidal composition containing, as an active ingredient, a mixture of quaternary ammonium compounds and a solvent, characterized in that it contains a mixture of three quaternary ammonium compounds with a total content of 19-21%, in the form of a mixture of a 50% aqueous solution of didecyldimethylammonium chloride containing 20 % 2-propanol, 50% aqueous solution of cocobenzyl dimethylammonium chloride containing 2% ethylene glycol, 30% aqueous solution of N, N-bis (3-aminopropyl) dodecylamine, and as a solvent, water and ethanol and / or isopropanol, as follows Component Content in wt.%:
50% didecyldimethylammonium chloride solution 5-25 50% solution of cocobenzyl dimethylammonium chloride 5-25 30% solution of N, N-bis (3-aminopropyl) dodecylamine 5-15 Ethanol and / or Isopropanol 18-22 Water Rest,
at a pH of the composition 5.5-6. 2. The method for producing the biocidal composition according to claim 1, characterized in that didecyldimethyl ammonium chloride is dissolved in 2-propanol and water is added to obtain a 50% solution, cocobenzyl dimethyl ammonium chloride is dissolved in water, ethylene glycol and water are added to obtain a 50% solution , dissolve N, N-bis (3-aminopropyl) dodecylamine in water to obtain a 30% solution, mix the resulting solutions in predetermined proportions, then add ethanol and / or isopropanol to the resulting mixture and, at the same time, water with vigorous stirring using Hovhan high speed propeller stirrer at a speed of 500 to 1000 rev / min for 3-5 min with water if necessary adjusted total composition to 100% and adjusted to pH 5.5-6. 3. The method according to claim 2, characterized in that the composition is additionally treated for 3-5 minutes with ultrasound at a frequency of 15-17 kHz.