[go: up one dir, main page]

RU2472853C2 - Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES - Google Patents

Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES Download PDF

Info

Publication number
RU2472853C2
RU2472853C2 RU2011101152/10A RU2011101152A RU2472853C2 RU 2472853 C2 RU2472853 C2 RU 2472853C2 RU 2011101152/10 A RU2011101152/10 A RU 2011101152/10A RU 2011101152 A RU2011101152 A RU 2011101152A RU 2472853 C2 RU2472853 C2 RU 2472853C2
Authority
RU
Russia
Prior art keywords
bacterium
bacterium according
aspartic acid
gene encoding
dehydrogenase
Prior art date
Application number
RU2011101152/10A
Other languages
Russian (ru)
Other versions
RU2011101152A (en
Inventor
Татьяна Михайловна Куваева
Сергей Васильевич Смирнов
Ольга Николаевна Иванова
Александр Дмитриевич Киверо
Жанна Иосифовна Каташкина
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) filed Critical Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ)
Priority to RU2011101152/10A priority Critical patent/RU2472853C2/en
Publication of RU2011101152A publication Critical patent/RU2011101152A/en
Application granted granted Critical
Publication of RU2472853C2 publication Critical patent/RU2472853C2/en

Links

Images

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

FIELD: chemistry.
SUBSTANCE: disclosed is Enterobacteriaceae bacteria family - producer of L-aspartic acid, which is modified such that said bacteria contain aspartate dehydrogenase. Disclosed is a method of producing L-aspartic acid, involving growing said bacteria in a culture medium and separating L-aspartic acid from the culture fluid.
EFFECT: invention enables to obtain L-aspartic acid in a large amount.
21 cl, 28 dwg, 22 tbl, 10 ex

Description

Текст описания приведен в факсимильном виде.

Figure 00000001
Figure 00000002
Figure 00000003
Figure 00000004
Figure 00000005
Figure 00000006
Figure 00000007
Figure 00000008
Figure 00000009
Figure 00000010
Figure 00000011
Figure 00000012
Figure 00000013
Figure 00000014
Figure 00000015
Figure 00000016
Figure 00000017
Figure 00000018
Figure 00000019
Figure 00000020
Figure 00000021
Figure 00000022
Figure 00000023
Figure 00000024
Figure 00000025
Figure 00000027
Figure 00000028
Figure 00000029
Figure 00000030
Figure 00000031
Figure 00000032
Figure 00000033
Figure 00000034
Figure 00000035
Figure 00000036
Figure 00000037
Figure 00000038
Figure 00000039
Figure 00000040
Figure 00000041
Figure 00000042
Figure 00000043
Figure 00000044
Figure 00000045
Figure 00000046
Figure 00000047
Figure 00000048
Figure 00000049
Figure 00000050
Figure 00000051
Figure 00000052
Figure 00000053
Figure 00000054
Figure 00000055
Figure 00000056
Figure 00000057
Figure 00000058
Figure 00000059
Figure 00000060
Figure 00000061
Figure 00000062
Figure 00000063
Figure 00000064
The text of the description is given in facsimile form.
Figure 00000001
Figure 00000002
Figure 00000003
Figure 00000004
Figure 00000005
Figure 00000006
Figure 00000007
Figure 00000008
Figure 00000009
Figure 00000010
Figure 00000011
Figure 00000012
Figure 00000013
Figure 00000014
Figure 00000015
Figure 00000016
Figure 00000017
Figure 00000018
Figure 00000019
Figure 00000020
Figure 00000021
Figure 00000022
Figure 00000023
Figure 00000024
Figure 00000025
Figure 00000027
Figure 00000028
Figure 00000029
Figure 00000030
Figure 00000031
Figure 00000032
Figure 00000033
Figure 00000034
Figure 00000035
Figure 00000036
Figure 00000037
Figure 00000038
Figure 00000039
Figure 00000040
Figure 00000041
Figure 00000042
Figure 00000043
Figure 00000044
Figure 00000045
Figure 00000046
Figure 00000047
Figure 00000048
Figure 00000049
Figure 00000050
Figure 00000051
Figure 00000052
Figure 00000053
Figure 00000054
Figure 00000055
Figure 00000056
Figure 00000057
Figure 00000058
Figure 00000059
Figure 00000060
Figure 00000061
Figure 00000062
Figure 00000063
Figure 00000064

Claims (21)

1. Бактерия семейства Enterobacteriaceae - продуцент L-аспарагиновой кислоты, модифицированная таким образом, что указанная бактерия содержит аспартатдегидрогеназу.1. A bacterium of the Enterobacteriaceae family is a producer of L-aspartic acid, modified in such a way that said bacterium contains aspartate dehydrogenase. 2. Бактерия по п.1, отличающаяся тем, что указанная бактерия модифицирована за счет введения гена, кодирующего аспартатдегидрогеназу.2. The bacterium according to claim 1, characterized in that said bacterium is modified by introducing a gene encoding aspartate dehydrogenase. 3. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Thermotoga maritima.3. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Thermotoga maritima. 4. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Polaromonas sp.4. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Polaromonas sp. 5. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Rhodopseudomonas palustris,5. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Rhodopseudomonas palustris, 6. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Ralstonia eutropha.6. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Ralstonia eutropha. 7. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Azorhizobium caulinodans.7. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Azorhizobium caulinodans. 8. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Bradyrhizobium japonicum.8. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Bradyrhizobium japonicum. 9. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Mesorhizobium sp.9. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Mesorhizobium sp. 10. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Corynebacterium glutamicum.10. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is derived from Corynebacterium glutamicum. 11. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Nitrosopumilus maritimus.11. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Nitrosopumilus maritimus. 12. Бактерия по п.2, отличающаяся тем, что ген, кодирующий аспартатдегидрогеназу, получен из Oceanicola granulosus.12. The bacterium according to claim 2, characterized in that the gene encoding aspartate dehydrogenase is obtained from Oceanicola granulosus. 13. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Pantoea.13. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Pantoea. 14. Бактерия по п.13, отличающаяся тем, что указанная бактерия принадлежит к виду Pantoea ananatis.14. The bacterium according to item 13, wherein the bacterium belongs to the species Pantoea ananatis. 15. Бактерия по п.13, отличающаяся тем, что указанная бактерия дополнительно модифицирована таким образом, что она имеет, по крайней мере:
- сниженную активность α-кетоглутаратдегидрогеназы;
- сниженную активность цитратсинтазы;
- увеличенную активность фосфоенолпируваткарбоксилазы или пируваткарбоксилазы;
- сниженную активность аспартатаммиаклиазы;
- сниженную активность пируваткиназы.15. The bacterium according to item 13, wherein the specified bacterium is additionally modified so that it has at least:
- reduced activity of α-ketoglutarate dehydrogenase;
- reduced activity of citrate synthase;
- increased activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase;
- reduced activity of aspartate ammonia lyase;
- reduced pyruvate kinase activity. 16. Бактерия по п.15, отличающаяся тем, что указанная бактерия дополнительно модифицирована таким образом, что она имеет сниженную активность аспартатаммиаклиазы (аспартазы).16. The bacterium according to clause 15, wherein said bacterium is further modified so that it has a reduced activity of aspartate ammonia lyase (aspartase). 17. Бактерия по п.15, отличающаяся тем, что указанная бактерия дополнительно модифицирована таким образом, что она имеет сниженную активность пируваткиназы.17. The bacterium according to claim 15, characterized in that said bacterium is further modified so that it has a reduced pyruvate kinase activity. 18. Бактерия по п.15, отличающаяся тем, что указанная бактерия дополнительно модифицирована таким образом, что она имеет сниженную активность малатдегидрогеназы.18. The bacterium according to clause 15, wherein said bacterium is further modified so that it has a reduced malate dehydrogenase activity. 19. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Escherichia.19. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Escherichia. 20. Бактерия по п.19, отличающаяся тем, что указанная бактерия принадлежит к виду Escherichia coli.20. The bacterium according to claim 19, characterized in that said bacterium belongs to the species Escherichia coli. 21. Способ получения L-аспарагиновой кислоты, включающий:
- выращивание бактерии по любому из пп.1-20 в питательной среде; и
- выделение из культуральной жидкости L-аспарагиновой кислоты. 21. A method for producing L-aspartic acid, comprising:
- growing bacteria according to any one of claims 1 to 20 in a nutrient medium; and
- isolation of L-aspartic acid from the culture fluid.
RU2011101152/10A 2011-01-14 2011-01-14 Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES RU2472853C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2011101152/10A RU2472853C2 (en) 2011-01-14 2011-01-14 Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2011101152/10A RU2472853C2 (en) 2011-01-14 2011-01-14 Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU2010101135/10A Substitution RU2010101135A (en) 2010-01-15 2010-01-15 BACTERIA OF THE ENTEROBACTERIACEAE FAMILY - PRODUCER OF L-ASAPPARATE OR METABOLITES, L-ASPARATE DERIVATIVES, AND METHOD OF PRODUCING L-ASAPPARATE OR METABOLITES, PRODUCED L-ASAPPARATE

Publications (2)

Publication Number Publication Date
RU2011101152A RU2011101152A (en) 2012-07-27
RU2472853C2 true RU2472853C2 (en) 2013-01-20

Family

ID=46850249

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2011101152/10A RU2472853C2 (en) 2011-01-14 2011-01-14 Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES

Country Status (1)

Country Link
RU (1) RU2472853C2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2805253C1 (en) * 2020-01-30 2023-10-13 СиДжей ЧеилДжеданг Корпорейшн New modified polypeptide with reduced citrate synthase activity and method for producing l-amino acid using it

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006254795A (en) * 2005-03-17 2006-09-28 Shimane Univ Aspartate dehydrogenase, alanine dehydrogenase, L-aspartate production method, and D-malate production method
JP2006254730A (en) * 2005-03-15 2006-09-28 Univ Of Tokushima Novel aspartate dehydrogenase
RU2337959C2 (en) * 2006-01-17 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) METHOD OF OBTAINING L-THREONINE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE yfeH IS INACTIVATED

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006254730A (en) * 2005-03-15 2006-09-28 Univ Of Tokushima Novel aspartate dehydrogenase
JP2006254795A (en) * 2005-03-17 2006-09-28 Shimane Univ Aspartate dehydrogenase, alanine dehydrogenase, L-aspartate production method, and D-malate production method
RU2337959C2 (en) * 2006-01-17 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) METHOD OF OBTAINING L-THREONINE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE yfeH IS INACTIVATED

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHIRU YANG, et al., "Aspartate Dehydrogenase, a Novel Enzyme Identified from Structural and Functional Studies of TM1643", THE JOURNAL OF BIOLOGICAL CHEMISTRY, Issue of March 7, 2003, Vol.278, No.10, pp.8804-8808. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2805253C1 (en) * 2020-01-30 2023-10-13 СиДжей ЧеилДжеданг Корпорейшн New modified polypeptide with reduced citrate synthase activity and method for producing l-amino acid using it

Also Published As

Publication number Publication date
RU2011101152A (en) 2012-07-27

Similar Documents

Publication Publication Date Title
RU2010101135A (en) BACTERIA OF THE ENTEROBACTERIACEAE FAMILY - PRODUCER OF L-ASAPPARATE OR METABOLITES, L-ASPARATE DERIVATIVES, AND METHOD OF PRODUCING L-ASAPPARATE OR METABOLITES, PRODUCED L-ASAPPARATE
TWI573869B (en) Introducing microorganisms with a fixed cycle of carbon dioxide
Li et al. Metabolic engineering of Corynebacterium glutamicum for methionine production by removing feedback inhibition and increasing NADPH level
RU2014127151A (en) METHOD FOR PRODUCING L-LYSINE USING MICRO-ORGANISMS WITH THE ABILITY TO PRODUCE L-LYSINE
BRPI0708008A2 (en) induction and stabilization of enzymatic activity in microorganisms
Ying et al. Optimization of culture conditions for enhanced lysine production using engineered Escherichia coli
RU2006122804A (en) NEW TYPES OF SCAR BACTERIA AND METHOD FOR PRODUCING AMBERIC ACID USING THE SPECIFIED BACTERIA
TWI642783B (en) Microorganism having carbon dioxide fixation cycle introduced thereto
WO2005030973A1 (en) Method of purifying succinic acid from fermentation liquid
CN103361389A (en) Method for aerobic production of alanine or a compound arising using alanine
RU2011116147A (en) METHOD FOR PRODUCING L-AMINO ACID OF THE GLUTAMATE FAMILY USING THE CORINEFORM BACTERIA
RU2018132244A (en) Microorganism for producing O-acetyl homoserine and a method for producing O-acetyl homoserine using this microorganism
RU2411289C2 (en) Pantoea genus bacteria, producer of l-aspartate or metabolite, which is derivative of l-aspartate, and method of producing l-aspartate or metabolite which is derivative of l-aspartate
RU2472853C2 (en) Enterobacteriaceae BACTERIA FAMILY - PRODUCER OF L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES, AND METHOD OF PRODUCING L-ASPARTIC ACID OR METABOLITES, L-ASPARTIC ACID DERIVATIVES
RU2018144165A (en) METHOD FOR ENZYMATIC L-LYSINE PRODUCTION
CN105073999B (en) The production method of 5-aminolevulinic acid or its salt
Conrady et al. Carboxylic acid consumption and production by Corynebacterium glutamicum
BRPI0513485A (en) process for the preparation of monomers and polymers thereof
WO2012157776A1 (en) Acrylamide aqueous solution, stabilization agent for acrylamide aqueous solution, and stabilization method for acrylamide aqueous solution
EP2151499A3 (en) Processes for the preparation of phosphatides
JP2009225705A (en) Method for producing theanine
US10294505B2 (en) Microorganism for production of chemicals derived from acetyl-CoA
Shen et al. Stereoselective nitrile hydratase
RU2322496C2 (en) Use of phosphoketolase for production of useful metabolites
JP6194255B2 (en) Process for producing 5-aminolevulinic acid or a salt thereof