RU2467989C2 - Method of treating organic wastes - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to agriculture. The method of treating organic wastes using vermiculture with Eisenia fetida earthworms, wherein a strain of Trichoderma asperellum MG-97 (VKPM F-765) in form of spores or mycelium or a preparation of trichodermin M are simultaneously added with the earthworms to the starting substrate in amount of 10⁵-10⁶ CFU/kg of the starting substrate.

EFFECT: invention improves quality of the obtained product, which lies in that the biopreparation has improved fungicidal and plant growth stimulation properties, as well as shorter period of treating organic wastes and high output of earthworm biomass.

12 tbl, 9 ex

Description

The invention relates to environmental biotechnology, the processing of organic waste using biological agents to obtain plant protection products and biomass of earthworms.

Known methods for processing organic waste for the production of biofertilizers using an industrial line of earthworms (California red hybrid, Eisenia fetida) (1, 2). The resulting biofertilizers are characterized by high fertilizing properties, but they do not have a significant effect on phytopathogenic soil microorganisms.

Microbial preparations based on strains of Bacillus subtilis (3, 4) Trichoderma hamatum (5) and a number of other bacteria and fungi are known to protect plants from diseases.

There is a method in which organic waste is processed and a suspension of phytopathogenic antagonist microorganisms: Trichoderma hamatum and bacteria Pseudomonas maltophila or Flavobacterium is added to the resulting compost (6). Compost obtained by this method has low fertilizing properties and does not stimulate plant growth.

The closest analogue to the present invention is a method of processing organic waste using earthworms Eisenia fetida to obtain a biological product, which consists in the fact that Bacillus subtilis bacteria or Trichoderma viride fungus are introduced into the finished biological fertilizer. However, this method does not change the duration of vermicomposting, the productivity of growing earthworms (growth and reproduction efficiency), a large number of microorganisms (bacteria or microscopic fungi) must be introduced into biofertilizer, and it does not have properties that stimulate plant growth and development (7).

The technical result is to improve the quality of the resulting product, which consists in the fact that the biological product has increased fungicidal and plant growth promoting properties, as well as reduced processing time of organic waste and increased yield of worm biomass.

The goal is achieved by the fact that the processing of the substrate based on organic waste of agricultural, municipal and industrial enterprises is carried out using earthworms (California red hybrid) and microorganisms with fungicidal and plant growth promoting properties, as well as the ability to decompose organic polymer compounds.

The essence of the invention lies in the fact that at the initial stage, earthworms (California red hybrid, Eisenia fetida) and the Trichoderma asperellum MG-97 fungus strain are simultaneously introduced into a substrate based on organic wastes (animal manure, plant residues, agricultural and food waste products). VKPM F-765) or the Trichodermin-M preparation (8), which have fungicidal, plant growth-promoting properties and the ability to degrade lignocellulosic waste (9.10). The strain Trichoderma asperellum MG-97 (VKPM F-765) or the drug "Trichodermin-M" contribute in the amount of 10 ⁵ -10 ⁶ colony forming units per 1 kg of substrate. Vermicomposting is carried out for 40-60 days, as a result of which a biological product with high fungicidal and growth-stimulating properties and biomass of earthworms are obtained.

Strain MG-97 T. asperellum is a known strain (Strain for protection of conifers from root rot, RF Patent No. 2171580) and obtained by Sadykova B.C. as a result of breeding work on the grounds of productivity, fungicidal and plant growth-promoting activity and the ability to decompose waste from the woodworking and hydrolysis industries. Deposited in the All-Russian Collection of Industrial Microorganisms under the number F-765.

The biological product “Trichodermin-M” contains propagules of T. asperellum strain MG-97 (4 × 10 ⁶ CFU / kg). The Trichodermin-M biological product is obtained by surface solid-phase cultivation in plastic containers on fir bark pretreated with isopropanol for 14 days (10).

Example 1. A method of processing organic waste with the introduction of red California worms into the substrate

For vermicomposting, manure is used (cow, horse or their mixture with plant residues, straw, sawdust), rotted on an open concrete site for 20 days at a temperature of 20-60 ° С. During this period, organic residues are periodically watered to maintain humidity (60-80%) and turned over to optimize aeration and temperature.

After adjusting the pH = 6.5-7.5, red Californian worms (Eisenia fetida) are added to the obtained substrate at the rate of 40 g (50 individuals 3-4 cm in size) per 1 kg. Vermicomposting is carried out for 40-60 days at a temperature of 20-25 ° C and a humidity of 50-80%.

At the end of vermicomposting, the worms are separated, the preparation is dried under natural conditions and sieved through a sieve.

The finished product has the following composition:

Mass fraction of moisture - 50%

Organic matter (With org.) - 32-38%

pH - 6.5-7.5

Total nitrogen - 0.8%

Total phosphorus (P ₂ O ₅ ) - 0.9%

Total potassium (K ₂ O) - 0.9%

Nitrates (mg / 100 g) - 8.05-9.50

Ammonium (mg / 100 g) - 0.55-0.70

Exchange phosphorus (mg / 100 g) - 110.09-129.24

Mobile potassium (mg / 100 g) - 740.20-765.35

Cu (mg / 1000 g) - 82.49

Zn (mg / 1000 g) - 172.81

Cd (mg / 1000 g) - 0.59

Pb (mg / 1000 g) - 15.59

Ni (mg / 1000 g) - 20.38

Co (mg / 1000 g) - 10.96

Cr (mg / 1000 g) - 17.32

Mn (mg / 1000 g) - 545.84

E. coli titer less than 0.01

helminth eggs not identified

Weed seeds - less than 20 per 1 ton.

No pathogenic bacteria and phytopathogenic fungi, helminth eggs, and weed seeds were detected in the preparation (extremely low amount, several (5) times less than permissible for biohumus) (TU RSFSR No. 949-91). It is characterized by a high content of humified organic matter, easily accessible to plants of the mineral forms of nitrogen, phosphorus and potassium and is environmentally friendly.

The increase in the biomass of earthworms for 42 days is 28% per 1 kg of substrate (at a humidity of 50%). Earthworm biomass averages 51.2 g per 1 kg.

During vermicomposting of the fir bark, the finished product is characterized by similar indicators, with the exception of a higher content of organic substances (With org. - 45-49%).

Example 2. A method of processing organic waste with the addition of red California worms and the strain of the fungus Trichoderma asperellum MG-97 (VKPM F-765)

For vermicomposting, manure is used (cow, horse or their mixture with plant residues, straw, sawdust), rotted on an open concrete site for 20 days at a temperature of 20-60 ° С. During this period, organic residues are periodically watered to maintain humidity (60-80%) and turned over to optimize aeration and temperature.

After adjusting the pH = 6.5-7.5, red Californian worms (Eisenia fetidd) are added to the obtained substrate at the rate of 40 g (50 individuals 3-4 cm in size) per 1 kg and T. asperellum strain MG-97 (1 × 10 ⁶ CFU / kg) as an aqueous suspension of spores and mycelium. Vermicomposting is carried out for 40-60 days at a temperature of 20-25 ° C and a humidity of 50-80%.

At the end of vermicomposting, the worms are separated, the biological product is dried under natural conditions and sieved through a sieve.

The finished biological product has the following composition:

Mass fraction of moisture - 50%

Organic matter (With org.) - 26-27%

pH - 6.5-7.5

Total nitrogen - 0.8%

Total phosphorus (P ₂ O ₅ ) - 0.9%

Total potassium (K ₂ O) - 0.9%

Nitrates (mg / 100 g) - 8.75-9.00

Ammonium (mg / 100 g) - 0.60-0.65

Exchange phosphorus (mg / 100 g) - 130.10-150.35

Mobile potassium (mg / 100 g) - 750.22-770.45

Cu (mg / 1000 g) - 80.49

Zn (mg / 1000 g) - 170.81

Cd (mg / 1000 g) - 0.60

Pb (mg / 1000 g) - 15.60

Ni (mg / 1000 g) - 20.40

Co (mg / 1000 g) - 10.90

Cr (mg / 1000 g) - 17.30

Mn (mg / 1000 g) - 545.90

E. coli titer less than 0.01

helminth eggs not identified

Weed seeds - less than 20 per 1 ton.

Titre T. asperellum - 1 × 10 ⁸ -2 × 10 ⁸ CFU / kg

Pathogenic bacteria and phytopathogenic fungi, helminth eggs, and weed seeds were not detected in the biological product (extremely low amount, several (5) times less than permissible for biohumus) (TU RSFSR No. 949-91). It is characterized by a high content of humified organic matter, easily accessible to plants of the mineral forms of nitrogen, phosphorus and potassium and is environmentally friendly.

The population density of trichoderma in the final product is 1-2 orders of magnitude higher than that introduced in the initial substrate, and is 1 × 10 ⁸ -2 × 10 ⁸ CFU / kg of biological product, which causes its increased fungicidal and growth-promoting properties.

The increase in the biomass of earthworms for 42 days is 40% per 1 kg of substrate. The biomass of earthworms averages 57 g per 1 kg of biological product.

Example 3. The method of processing organic waste with the introduction of red California worms and the drug "Trichodermin-M" on the bark of fir

For vermicomposting, manure is used (cow, horse or their mixture with plant residues, straw, sawdust), rotted on an open concrete site for 20 days at a temperature of 20-60 ° С. During this period, organic residues are periodically watered to maintain humidity (60-80%) and turned over to improve aeration and reduce temperature.

Red Californian worms (Eisenia fetida) are added to the prepared substrate at the rate of 40 g (50 individuals 3-4 cm in size) per 1 kg and the Trichodermin-M biological product based on T.asperellum MG-97 strain in the amount of 1 × 10 ⁵ -4 × 10 ⁶ CFU of the fungus per 1 kg of the initial substrate.

Vermicomposting is carried out for 42 days at a temperature of 20-25 ° C and a humidity of 50-80%. At the end of the worms are separated, the biological product is dried in vivo and sieved through a sieve.

The finished biological product has the following composition:

Mass fraction of moisture - 50%

Organic matter (With org.) - 40-46%

pH - 6.5-7.5

Total nitrogen - 0.8%

Total phosphorus (P ₂ O ₅ ) - 0.9%

Total potassium (K ₂ O) - 0.9%

Nitrates (mg / 100 g) - 9.60-9.70

Ammonium (mg / 100 g) - 0.23-0.29

Exchange phosphorus (mg / 100 g) - 105.11-125.29

Mobile potassium (mg / 100 g) - 500.50-524.50

Cu (mg / 1000 g) - 82.40

Zn (mg / 1000 g) - 172.90

Cd (mg / 1000 g) - 0.60

Pb (mg / 1000 g) - 15.50

Ni (mg / 1000 g) - 20.30

Co (mg / 1000 g) - 10.90

Cr (mg / 1000 g) - 17.30

Mn (mg 1000 g) - 545.80

E. coli titer less than 0.01

helminth eggs not identified

Weed seeds - less than 20 per 1 ton.

Title T. asperellum - 1 × 10-2 × 10 ⁸ CFU / kg

In the biological product, pathogenic bacteria and phytopathogenic fungi, helminth eggs and weed seeds were not detected (extremely low quantity, less than 20 seeds / t, 5 times lower than according to TU RSFSR No. 949-91). It is characterized by a high content of humified organic matter, easily accessible to plants of the mineral forms of nitrogen, phosphorus and potassium and is environmentally friendly.

The population density of trichoderma in the final product - bio-fertilizer is 1-2 orders of magnitude higher than that introduced in the original substrate, and is 1 × 10 ⁸ -2 × 10 ⁸ CFU / kg of biological product, which causes its increased fungicidal and growth-promoting properties. The increase in the biomass of earthworms for 42 days is 45% per 1 kg of substrate. The biomass of earthworms averages 58 g per 1 kg of the resulting biological product.

Example 4. The fungicidal properties of the biological product obtained according to example 2 (vermicomposting with the introduction of the strain Trichoderma asperellum MG-97 (VKPM F-765))

To evaluate the fungicidal properties of a biological product, it is applied to the soil in a ratio of 1: 5 by volume. The experiments are carried out in pots of 500 g of soil mixture in each. The repetition of experiments three times. In each pot, seeds of Rodnichok cucumbers previously soaked in water are sown. The study is carried out on an artificial infectious background, for which a phytopathogen inoculum is introduced into the soil mixture. To assess the effect of the drug, two strains of phytopathogens are introduced into the soil before sowing, in an amount that causes 100% death of plants in the control. The infectious load is 5 × 10 ⁶ CFU / g of soil for Fusarium sporotrichioides and 1 × 10 ⁵ CFU / g for Alternaria sp. Cucumber seeds are sown in prepared soil mixtures. Plants are incubated in conditions of full round-the-clock illumination and humidity of at least 80%. Take into account the germination and post-emergence death (attack) seedlings, the results are presented in tables 1 and 2.

Obtained by the proposed method of vermicomposting, a biological product increases the resistance of cucumber plants to damage by root phytopathogens - fusaria and alternaria. In comparison with the prototype, the germination of cucumbers increases from 55% to 100% in the presence of a Fusarium infection in the soil (table 1) and from 88% to 100% in the soil with phytopathogenic - alternaria. Post-emergence death of seedlings is reduced from 55% to 22% in soil with Fusaria and from 22% to 10% by alternaria. In contrast to the prototype, the resistance of seedlings to root infections increases significantly, and the drug obtained by the proposed method is effective against an infectious background of phytopathogens, leading to 100% death of plants.

Table 1 Fungicidal activity of a biological product to root Fusarium pathogens Options / day Germination,% Post-emergence death,% 2 5 10 fifteen 10 fifteen thirty The closest analogue obtained by vermicomposting without making mushrooms 0 55 one hundred one hundred 0 0 0 Biological product obtained by vermicomposting with T. asperellum 40 55 one hundred one hundred 0 0 0 The closest analogue obtained by vermicomposting without making fungi against the infectious background of F.sporotrichioides 0 thirty 55 55 10 thirty 55 Biological product with T. asperellum on an infectious background of F. sporotrichioides 0 thirty 60 one hundred 10 22 22

table 2 Fungicidal activity of the biological product to root rot caused by phytopathogens Alternaria sp. Options / day Germination,% Post-emergence death,% 2 5 10 fifteen 10 fifteen thirty The closest analogue obtained by vermicomposting without making mushrooms 0 55 one hundred one hundred 0 0 0 Biological product obtained by vermicomposting with T. asperellum 40 55 one hundred one hundred 0 0 0 The closest analogue obtained by vermicomposting without making fungi against an infectious background Alternaria sp. 40 55 88 88 10 22 22 Biological product obtained by vermicomposting with trichodermin on an infectious background Alternaria sp. 0 thirty one hundred one hundred 10 0 10

Example 5. The fungicidal properties of the biological product obtained according to example 3 (vermicomposting with the drug Trichodermin-M)

To evaluate the fungicidal properties of a biological product, it is applied to the soil in a ratio of 1: 5 by volume. The experiments are carried out in pots of 500 g of soil mixture in each. The repetition of experiments three times. In each pot, seeds of Rodnichok cucumbers previously soaked in water are sown. The study is carried out on an artificial infectious background, for which a phytopathogen inoculum is introduced into the soil mixture. To assess the effect of biological products, a phytopathogen strain is introduced into the soil before sowing in an amount that causes 100% plant death in the control. The infectious load is 5 × 10 ⁶ CFU / g of soil for Fusarium sporotrichioides and 1 × 10 ⁵ CFU / g for Alternaria sp. Cucumber seeds are sown in prepared soil mixtures. Plants are incubated in conditions of full round-the-clock illumination and humidity of at least 80%. Take into account the germination and post-emergence death (attack) seedlings, the results are presented in tables 3 and 4.

Table 3 Fungicidal activity of a biological product obtained by vermicomposting with trichodermin-M to root Fusarium pathogens Options / day Germination,% Post-emergence death,% 2 5 10 fifteen 10 fifteen thirty The closest analogue obtained by vermicomposting without making mushrooms 0 55 one hundred one hundred 0 0 0 Biological product obtained by vermicomposting with trichodermin twenty 55 one hundred one hundred 0 0 0 The closest analogue obtained by vermicomposting without making fungi against the infectious background of F.sporotrichioides 0 thirty 55 60 10 thirty 55 Biological product obtained by vermicomposting with trichodermin against the infectious background of F. sporotrichioides 0 22 60 one hundred 10 0 0

Table 4 Fungicidal activity of a biological product obtained by vermicomposting with trichodermin-M to root rot caused by alternaria Options / day Germination,% Post-emergence death,% 2 5 10 fifteen 10 fifteen thirty The closest analogue obtained by vermicomposting without making mushrooms 0 55 one hundred one hundred 0 0 0 Biological product obtained by vermicomposting with trichodermin twenty 55 one hundred one hundred 0 0 0 The closest analogue obtained by vermicomposting without making fungi against an infectious background Alternaria sp. 0 55 88 88 10 22 22 Biological product obtained by vermicomposting with trichodermin on an infectious background Alternaria sp. 0 40 60 one hundred 0 0 0

Example 6. The influence of the method of vermicomposting on the density of populations of phytopathogens in the biological product

The estimation of the number of phytopathogens in the preparation obtained by the proposed method of vermicomposting is carried out according to examples 1 and 2 and against the background of introducing strains of phytopathogenic fungi into the initial substrate in an amount that causes 100% plant death. The infectious load on the substrate for vermicomposting the phytopathogen Fusarium sporotrichioides is 5 × 10 ⁶ CFU / g and Alternaria sp. - 1 × 10 CFU / g of substrate. In microbiological crops for Chapek and wort-agar nutrient media, changes in the density of the phytopathogen population in the substrate are taken into account during vermicomposting and the final product is a biological product.

When vermicomposting according to the proposed method, in contrast to the prototype, the death of phytopathogenic fungi is much more effective, since trichoderma is added to the original substrate. In the resulting biological product, the population density of phytopathogens introduced into the initial substrate is several times (2-5 times) lower than in the biological fertilizer according to the prototype (tables 5 and 6).

Example 7. Stimulation of the growth and development of plants with the biological product obtained in example 2 (vermicomposting with the introduction of the strain of the fungus Trichoderma asperellum MG-97 (VKPM F-765))

To study the properties of a biological product to stimulate plant growth, it is introduced into the soil in a ratio of 1: 5 by volume. The experiments are carried out in pots of 500 g of soil mixture in each. The repetition of experiments three times. The study is carried out in soil without and with an artificial infectious background, for which a phytopathogen inoculum is introduced into the soil mixture. The infectious load is 5 × 10 ⁶ CFU (micro and macroconidia) / g of soil for Fusarium sporotrichioides and 1 × 10 ⁵ CFU / g for Alternaria sp. Pre-soaked seeds of Rodnichok cucumbers are sown in prepared soil mixtures. Plants are incubated in conditions of full round-the-clock illumination and relative humidity of at least 80%. After growing for one month, the cucumber plants are weighed and their morphometric indicators are determined: the number of leaves, the length of the aerial and root systems, the total biomass of the aerial parts of the plants and the average biomass of one plant (tables 7 and 8). The data presented in tables 5 and 6 show that the biological product has a growth-promoting effect, increasing the aerial part and the root system. The growth-promoting effect of the drug obtained by the proposed method is significantly higher than that of the prototype. The most powerful effect it has on the formation of leaves and root systems of plants.

Example 8. The efficiency of processing organic substrates with the proposed method of vermicomposting

The effect of the Trichoderma asperellum strain, introduced in the form of an aqueous suspension, and the Trichodermin-M preparation based on it on the decomposition of organic substrates using the proposed method of vermicomposting are studied. The strain Trichoderma asperellum MG-97 has laccase (80 units / g a.s.), peroxidase (40 units / g a.s.) and cellulolytic (270 units / g a.s. ) activities, which determines its ability to efficiently destroy complex organic substances, including lignocellulosic compounds predominant in plant residues (8).

The ability of the strain Trichoderma asperellum and the drug "Trichodermin-M" to intensify the process of vermicomposting is evaluated by the content of organic carbon and mineral forms of nitrogen (nitrates and ammonium) in the resulting preparation according to the proposed method in comparison with the prototype. At the same time, the total number of microscopic fungi, which are the leading destructors of plant polymer compounds, in substrates subjected to vermicomposting is estimated.

The addition of the Trichoderma asperellum strain and the Trichodermin-M preparation to the initial substrate increases the number of colony forming units of fungi in the substrate and accelerates the mineralization of organic compounds (Tables 9 and 10). This leads to a greater decrease in organic carbon during vermicomposting and, accordingly, its lower content in the biological product and the accumulation of more inorganic forms of nitrogen in it than when bio-fertilizing according to the prototype.

Table 9 The number of microscopic fungi in the substrate during vermicomposting in a biological product Options The number of CFU x 10 ⁶ 0 days 7 days 14 days 42 days The closest analogue obtained by vermicomposting without making mushrooms 0.003 ± 0.001 0.21 ± 0.01 0.84 ± 0.01 0.51 ± 0.01 Biological product obtained by vermicomposting with trichodermin 1.003 ± 0.001 3.23 ± 0.04 3.96 ± 0.04 2.94 ± 0.04 Biological product with T. asperellum 1.003 ± 0.001 2.45 ± 0.01 2.87 ± 0.01 2.12 ± 0.01

Table 10 The content of organic carbon and mineral forms of nitrogen in the biofertilizer obtained in example 1 and 2 Option / Metric Sorg *,% Nmin, mg / 100 g The closest analogue obtained by vermicomposting without making mushrooms 36.6 9.40 Biological product obtained by vermicomposting with T. asperellum 26.5 9.88 The closest analogue obtained by vermicomposting without introducing mushrooms with fir bark (control for the variant with the introduction of the drug) 47.6 9.20 Biological product obtained by vermicomposting with the drug "Trichodermin-M" 43.0 10.93 * - organic carbon content (calculated on dry weight) in the initial substrate before vermicomposting - 66.2-67.0%.

Example 9. The productivity of earthworms with the proposed method of vermicomposting

The specifics of the action of the antagonist Trichoderma asperellum strain MG-97, introduced in the form of an aqueous suspension, and the drug "Trichodermin-M" on the growth and development of worms are studied. The influence of the antagonist strain and the biological product based on it is evaluated by the dynamics of biomass accumulation and the proportion of mature worms in the population. Counts of earthworms are carried out on 0, 7, 14 and 42 days.

The addition of the antagonist strain and the Trichodermin-M preparation to the substrate at the beginning of vermicomposting and the use of mushroom propagules in food positively affect the growth, biomass accumulation, and the rate of formation of sexually mature individuals in the E. fetida worm population (Tables 11 and 12). The earthworm biomass increases during vermicomposting according to the prototype from 40.0 to 51.2 g / kg (28%) of the obtained biofertilizer, and according to the proposed method, from 40 to 57.06 g / kg (43%) with Trichoderma asperellum and from 40 to 58.14 g / kg with trichodermin-M (by 45%). The proportion of sexually mature individuals of earthworms is also higher when vermicomposting substrates by the proposed method than the prototype. After 2 weeks and until the completion of vermicomposting, it is 45-50%, and according to the prototype 31-40% in the population of E. fetida. The number of cocoons deposited by earthworms during vermicomposting is 15-25% higher with the proposed method compared to the prototype.

The data obtained show that by introducing Trichoderma asperellum and the drug "Trichodermin-M" in the initial substrate, the effectiveness of vermiculture is increased (tables 11 and 12).

So, the proposed method of vermicomposting allows more efficient processing of organic waste (reduce time), increase the production of earthworms and the proportion of mature individuals in it, and obtain a biological product that has an increased suppressive effect on phytopathogens and growth-promoting on plants.

Literature

1. Striganova B.R. Humus and its creators. / Household economy, No. 6, 1991. - S.12-16.

2. Dondokova VB The influence of environmental conditions on the cultivation of the earthworm Eisenia fetida upon receipt and use of vermicompost. / Abstract. diss. Cand. biol. sciences. Ulan-Ude. 2006 .-- 23 p.

3. French patent No. 8113118.1981.

4. Patent of Russia No.2019966 C1, cl. A / 01 63/00, 1991.

5. French patent No. 2545099, C12N 1/14, 1984.

6. US patent No. 4642131, CL. C05F 11/08, 1981.

7. Patent of Russia No. 2125549 C1F 11/08, 1999.

8. Sadykova B.C., Gromovy TI, A.N. Likhachev, A.V. Kurakov A.V. The biological activity of Siberian strains of Trichoderma as a selection factor for the creation of new generation plant protection biological products. / Biotechnology No. 6, 2007, S.12-17.

9. Tretyakova I.N., Sadykova B.C., Cooper P.N., Noskova N.V. Growth-stimulating activity of strains of the genus Streptomyces and Trichoderma and prospects for their use for microclonal propagation of conifers. / Biotechnology No. 1, 2009 - S.39-45.

10. Sadykova B.C., Bondar P.N., Tretyakova I.N., Noskova N.E. Antagonistic and growth-promoting activity of strains of the genus Trichoderma and prospects for their use in biocontrol. / Immunology, allergology, infectology, t.2, 2009. - p.206-207.

11. Gaydasheva II, Gromovy TI, Sadykova B.C., Zobova N.V., Bondar P.N. Prospects for the use of biological products for plant protection in the cultivation of cereals in Central Siberia. / Bulletin of the Krasnoyarsk State Agrarian University, T.1, 2008. - P.27-32.

12. TU of the RSFSR No. 949-91.

Claims (1)

A method of processing organic waste using vermiculture with Eisenia fetida earthworms, characterized in that the Trichoderma asperellum MG-97 strain (VKPM F-765) is introduced simultaneously with the worms in the form of spores and mycelium or trichodermin-M in the amount of 10 ⁵ -10 ⁶ CFU / kg of starting substrate.