RU2337669C2 - Method for stomach ulcer treatment in young pig - Google Patents

Abstract

FIELD: medicine, veterinary medicine.

SUBSTANCE: method prescribes introducing of encapsulated "Biopag-D" preparation into young pig fodder, in the form of 0.01% solution, by 1.5 g capsules to 1 kg body weight daily, during 7 days.

EFFECT: invention allows appreciable sanitation of pigs' livestock, significant lowering loss and weight increase retardation; enables obtaining healthy posterity from these young pigs in future.

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Description

The invention relates to veterinary medicine, namely to gastroenterology.

Analogues of the proposed method for the correction of peptic ulcer (UB) of the stomach and duodenal ulcer in piglets do not exist.

There are recommendations in the literature on the use of Biopag-D, such as disinfection of premises, irrigation of root crops to preserve them during storage, treatment of skin in order to prevent and treat skin infections in animals (Efimov K.M., Gembitsky P.A. , Snezhko A.G. Polyguanidines - a class of low-toxic long-acting disinfectants // Disinfection Business, 2000. - No. 4. - p.32-36, www.ruscience.newmail.ru/journals/jmed.htm; Naumov M.M. , Medvedev I.N., Efimov K.M. et al. Practical recommendations for the use of Biopag-D in veterinary medicine. Moscow, 2006. - 22 pp.). However, the drug has never been used to treat ulcer in piglets.

It is known that against the background of standard feeding in pigsties, by 1 year of life, in 70% of cases pigs develop YAB. It is the reason for the decrease in weight gain and often their death due to the development of bleeding, perforation or penetration of the ulcer. The reason for the development of peptic ulcer is the colonization of the gastrointestinal tract of piglets Helicobacter pilori, which upsets the balance between the acid-forming and barrier functions of the animal’s stomach in favor of the former, weakening the body’s defenses, increasing the likelihood of animal death.

The aim of the invention is to develop an effective approach to the treatment of ulcer in piglets.

The essence of the proposed method lies in the fact that for the treatment of ulcer, Biopag-D is added to piglet feed 0.01% in capsule form (capsules from gelatin) at the rate of 1.5 g capsules per 1 kg of animal body weight per day for 7 days. This allows sanitation of the gastrointestinal tract of the piglet from N. Pylori and in 70% to ensure scarring of the ulcer, approaching 100% by the 10th day of observation.

The inventive method is as follows. In the beginning, gelatin capsules are prepared for pouring the Biopag-D solution. The gelatin mass is prepared. Gelatin, freshly boiled hot water, glycerin in a ratio of 2.5: 6: 1 are added to a flask with a capacity of 50 ml and the mass is heated in a water bath at a temperature of 90-95 ° С. Mixing during dissolution should be careful, otherwise air enters the viscous solution. To remove air, the solution is kept without stirring for 30 minutes at a temperature of 45 ° C, after which it is poured into a porcelain crucible.

Then carry out the formation of soft gelatin shells and drying of the capsules. Metal forms (olives) with a diameter of 5 mm are wiped with a gauze swab moistened with liquid paraffin, cooled at a temperature of 3-5 ° C for 5-6 minutes and immersed in a gelatinous mass at a temperature of 38-40 ° C for 1-2 s.

To evenly distribute the mass and to prevent sagging, the molds are smoothly lifted by rotating in a horizontal position around its axis. With this technology for the production of capsules, their wall thickness does not exceed 1 mm. Forms with gelatin films are refrigerated for 5-7 minutes at 5 ° C. After this, the capsules are carefully cut with a scalpel (blade) at the base of the olive. Capsules are carefully removed from the mold, removed and placed in the nest of the cachet machine.

The capsules are filled with a solution of 0.01% Biopag-D using a syringe with a curved cannula, which is inserted into the opening of the capsule without wetting the edges. A solution of a 0.01% Biopag-D solution is prepared by dissolving 1 g of Biopag-D in 10 liters of distilled water.

The capsule opening is sealed with an electric soldering iron heated to a temperature of 55-56 ° C (voltage 12 V). The molten film seals the capsule due to surface tension. Sealing should be smooth, rounded and without sagging. For gelatin dehydration, the filled soft capsules are dried at a temperature of 20-23 ° C in a desiccator over calcined calcium chloride.

To calculate the enclosed Biopag-D capsule, the following chain of reasoning is necessary. Since the capsule is a sphere with a diameter of 7 mm and a radius of 3.5 mm, and the cavity filled with a solution of a sphere with a diameter of 5 mm and a radius of 2.5 mm, their volume is calculated by the formula (Pogorelov A.V. Geometry. Textbook for 6- 10 classes, M .: Education, 1985):

then the volume of one capsule with the solution is:

and volume

the solution contained in one capsule is

The volume of one gelatin capsule is equal to the difference of these volumes and is:

V _{gelatin capsule} = V _{capsule with a solution of} -V _{solution in the capsule} = 0.180-0.065 = 0.115 cm ³

Given that 1 cm ³ = 1 ml and that the density of gelatin capsules is 1.35 g / ml (Weiss A. Macromolecular chemistry of gelatin, M., 1971), we calculate the weight of the gelatin shell of one capsule:

0.115 ml × 1.35 g / ml = 0.152 g

Given that 1 cm ^{3 of an} aqueous solution has a mass of 1 g, we calculate the mass of one capsule as a whole:

m of _{one capsule with a solution} = 0.152 g + 0.065 g = 0.217 g

Moreover, the mass of Biopag-D contained in a 0.01% solution in one capsule is:

m _{“Biopag-D” in 1 capsule} = 0.065 g × 0.0001 = 0.0000065 g

According to the materials of the application for an invention, in order to achieve a technical result, it is necessary to give piglets 1.5 g of capsules per 1 kg of body weight. Given that 1 capsule has a mass of 0.217 g, then 1.5 g of them contains:

1.5 g: 0.217 g = 6.91 pcs, which contain 6.91 × 0.0000065 g = 0.00004 g

Thus, the technical result of the claimed invention is achieved using Biopag-D at a dose of 0.00004 g per 1 kg of piglet body weight.

After receiving the finished capsules, they are used in piglets for the treatment of ulcer. Capsules "Biopag-D" are mixed with animal feed and fed for 7 days at the rate of 1.5 g of capsules containing 0.00004 g of active substance per 1 kg of piglet body weight.

EFFECT: invention makes it possible to cure YB in piglets as a result of 7 day feeding of Biopag-D 0.01% in capsule form at the rate of 1.5 g of Biopag-D per 1 kg of animal body weight per day in almost 100% of cases. This allows you to significantly improve the number of pigs, significantly reduce mortality, delay in weight gain, optimizing meat production, and makes it possible to subsequently obtain healthy offspring from these piglets.

The availability of the method of treating ulceration in piglets will allow for the rapid and cost-effective treatment of animals, healing the herd.

Example 1. Piglet No. 26, 1 year 1 month, 85 kg, reduced the gain, began to refuse feed. The piglet underwent gastroscopic examination and established a stomach ulcer with a diameter of 1.5 cm and a depth of 0.5 cm along the lesser curvature. From the detritus covering the ulcer niche, a sample was taken for microscopy, in which the presence of Helicobacter pylori was detected. The pig was diagnosed with gastric ulcer with contamination of Helicobacter pylori. Piglet was prescribed 127.5 g of Biopag-D 0.01% capsules containing 0.0034 g of active ingredient per day for 7 days. This allowed 5 days later to normalize the animal’s appetite, its motor activity. Repeated gastroscopy on the 8th day after the start of treatment showed scarring of the ulcer with no ulcerative defect. Microscopic examination of the contents of the stomach did not reveal the presence of Helicobacter pylori.

Further monitoring of this pig did not reveal a deviation from the norm and the absence of a recurrence of ulcer.

Example 2. Piglet No. 32, 1 year 3 months, 96 kg, reduced the gain, began to refuse feed. The piglet underwent gastroscopic examination and established a stomach ulcer with a diameter of 1.2 cm and a depth of 0.3 cm in the antrum. From the detritus covering the ulcer niche, a sample was taken for microscopy, in which the presence of Helicobacter pylori was detected. The pig was diagnosed with gastric ulcer with contamination of Helicobacter pylori. The animal was prescribed 144 g of Biopag-D capsules 0.01%, containing 0.00384 g of active ingredient, per day for 7 days. This allowed after 4 days to normalize the animal’s appetite, its motor activity. Repeated gastroscopy on the 9th day after the start of treatment showed complete scarring of the ulcer with no ulcerative defect. Microscopic examination of the stomach contents of the animal did not reveal Helicobacter pylori.

Further monitoring of the pig did not reveal a deviation from the norm and the absence of recurrence of ulcer.

Claims (1)

A method for treating peptic ulcer disease in piglets, comprising the use of a Biopag-D capsule enclosed in a 0.01% solution of 1.5 g capsules per 1 kg of animal body weight per day for 7 days when mixed with food.