RU2366439C1 - Method for making precipitated preparation of shelf fungus - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: diffusion juice of shelf fungus is prepared and precipitated by adding 20% hydrochloric acid solution to pH 2.0-2.2. Before the precipitation stage, aqueous solution of hyperbranched polymer concentrated 1*10^-7-1*10^-20 % is introduced into shelf fungus juice in ratio 10:1. Processing is performed at room temperature within 0.5-1.5 hours.

EFFECT: lower impurity content in a product, higher yield and antioxidant activity of a product.

1 tbl, 2 ex

Description

The invention relates to the pharmaceutical, food and cosmetic industries and for obtaining a precipitated preparation from birch fungus chaga. A known method for determining the integral antioxidant capacity of biological fluids (patent No. 2253114, IPC ⁷ G01N 33/48, authors Pogoreltsev VI, Ziyatdinova GK, Budnikov GK Patent holder Kazan State Medical University).

A known method of obtaining funds with antioxidant activity (patent No. 2203077, IPC ⁷ A61K 35/78, A61P 39/06, authors Ermilova E.V., Krasnov E.A., Pisareva S.I., Kadirova T.V., applicant Siberian State Medical University).

The prototype is a method of producing a precipitated preparation from chaga birch mushroom (A.S. No. 126988, authors N.A. Yakimov, S.M. Andreeva, E.V. Alekseeva and N.L. Mattison).

The disadvantage of these inventions is the high content of ash and other ballast impurities, the low yield of the polyphenolic complex and its low antioxidant activity.

The objective of the proposed invention is to reduce the content of ash and other ballast impurities, increase the yield of the polyphenolic complex and increase its antioxidant activity.

This object is achieved in that in the method for producing a precipitated preparation from birch fungus fungus, by producing diffusion fungus juice, precipitating it, adding a 20% hydrochloric acid solution to a pH of 2.0-2.2, according to the invention, to the precipitation stage Chaga diffusion juice is added an aqueous solution of a hyperbranched polymer with a concentration of 1 · 10 ^-7 -1 · 10 ^-20 , in a ratio of 10: 1, processing is carried out at room temperature for 0.5-1.5 hours.

A method of obtaining an alcohol extract of chaga is as follows.

Chaga diffusion juice obtained from 10 g of raw material is treated with an aqueous solution of hyperbranched Boltorn 30 polymer with a concentration of 1 · 10 ^-7 -1 · 10 ^-20 %, using the ratio of diffusion juice: Boltorn 30 hyperbranched polymer solution 10-10: 1 at room temperature in within 0.5-1.5 hours. The polyphenol complex is precipitated with a 20% solution of hydrochloric acid to a pH of 2.0-2.2 and a precipitate of the PFA polyphenolic complex is obtained (1). Its mass, antioxidant capacity (AOE) and ash content are determined.

Example 1. Chaga diffusion juice, obtained from 10 g of raw materials, is treated with an aqueous solution of hyperbranched Boltorn 30 polymer with a concentration of 1 · 10 ^-20 %, using the ratio of diffusion juice: solution of hyperbranched polymer Boltorn 30 - 10: 1 at room temperature for 0, 5-1.5 hours. The polyphenol complex is precipitated with a 20% hydrochloric acid solution to a pH of 2.0-2.2, 1.57 g of a precipitate is obtained - PFC (1), the ash content of which is 3.55%. The antioxidant capacity (AOE) of the isolated polyphenolic complex - PFC (1) is 64 ± 3.5 kCl / 100 g, S _r = 0.05.

Example 2. Chaga diffusion juice, obtained from 10 g of raw material, is treated with an aqueous solution of hyperbranched polymer Boltorn 30 with a concentration of 1 · 10 ^-12 %, using a ratio of 10: 1 at room temperature for 1 hour. The polyphenol complex is precipitated with a 20% hydrochloric acid solution to a pH of 2.0-2.2, 1.83 g of the precipitate is obtained - polyphenol (1), the ash content of which is 3.21%. The antioxidant capacity (AOE) of the isolated polyphenolic complex - PFC (1) is 39 ± 3 kC / 100 g, S _r = 0.05.

The results of multiple experiments were calculated using the program "Statistics 6", which is reflected in the data of table 1, at P = 0.95, n = 5.

Table 1 The use of hyperbranched polymer Boltorn 30 to obtain a precipitated preparation from birch fungus fungus The concentration of the polymer BH 30,% PFC weight AOE PFK, kCl / 100 g PFK PFC ash g %, to raw materials g %, by weight of PFC 0 1.5417 ± 0.0280 15.42 31 ± 1 0.2875 ± 0.0012 4.8 10 ^-4 1.4913 ± 0.0272 14.91 30 ± 1 * 10 ^-7 1.5362 ± 0.0199 15.36 39 ± 3 * 10 ^-12 1.8269 ± 0.0333 18.27 32 ± 1 0.2698 ± 0.0009 3,55 10 ^-16 1.5102 ± 0.0027 15.10 47 ± 2 * 10 ^-20 1.5688 ± 0.0184 15.69 64 ± 3,5 0.2371 ± 0.0009 3.21 10 ^-22 1.4034 ± 0.0212 14.03 31 ± 3 * Note: * - not determined.

Hyperbranched polymers, in particular Boltorn 30, are designed to deliver bioactive molecules to target tissues (Javier Rojo, Rafael Delgado - Glicodendritic structures: promising new antiviral drugs, Journal of Antimicrobial Chemotherapy, vol. 54, No. 3, p.579-581) . Having a large volume of the molecule and a large number of hydroxyl groups at its periphery, hyperbranched polymers are able to interact with substances of hydrophilic and hydrophobic nature, as well as with metal cations. Chaga diffusion juice is a colloidal polydisperse system, the dispersed phase of which is represented by a polyphenolic complex. The introduction of a low concentration of Boltorn 30 hyperbranched polymer into this colloidal system at a low concentration leads to the rearrangement of both the dispersion medium and the dispersed phase (polyphenol complex). Due to the restructuring of the colloidal system of chaga diffusion juice, when applying Boltorn 30, there is a decrease in the content of ash and other ballast impurities, an increase in the yield of the polyphenolic complex and an increase in its antioxidant activity.

Using the alleged invention allows, in comparison with the control:

- reduce the content of ash and other ballast substances that make up the polyphenol complex by 33% (example 1) and 26% (example 2);

- increase the yield of the polyphenolic complex by 18.5% (example 2);

- to increase the antioxidant activity of the polyphenolic complex in half (example 1).

The invention can be used to obtain, on its basis, drugs, dietary supplements, functional foods and cosmetics.

Claims (1)

A method of obtaining a precipitated preparation from chaga birch fungus by obtaining chaga diffusion juice, precipitating its addition of a 20% hydrochloric acid solution to a pH of 2.0-2.2, characterized in that an aqueous solution of hyperbranched is added to the chaga diffusion juice polymer with a concentration of 1 · 10 ^-7 -10 ^-20 %, in a ratio of 10: 1, processing is carried out at room temperature for 0.5-1.5 hours