RU2354405C2 - Disinfecting means - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine and sanitation, and can be used for disinfection in medical-prophylactic institutions of different type, units of communal services, on any transport. Disinfectant, includes polymeric organic substance, bactericidal additive - 2-methyl-5-[di(trifluorinemethyl)oximethyl]furane and ethanol, as polymeric organic substance it contains 50% water solution of vinyl pyrrolidone copolymer with vinyl acetate, with definite component ratio.

EFFECT: invention allows saving high disinfecting properties of means during 26 months, is highly efficient against bacterial (including tuberculosis bacteria), viral infections, pathogenic fungi and belongs to low-hazard substances.

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Description

The invention relates to sanitation, relates to disinfectants, can be used for disinfection of small in area and difficult to process surfaces in rooms, hard furniture, equipment, apparatus, devices in medical institutions of various profiles, as well as offices, communal services, any transport.

Known disinfectant "Vilagin" (RF Patent No. 1522498, A61L 2/16 from 03/09/94). The disadvantage of this tool is its low solubility in water; in terms of acute toxicity, it refers to substances of the 3rd hazard class - moderately hazardous (GOST 12.1.007-76 "Harmful substances. Classifications and general safety requirements"). Vilagin in its native form and in alcohol solutions (60-80%) has an irritating effect on the skin, has an irritating effect on the mucous membranes. In addition, during prolonged storage (more than 3 months), it oxidizes in the light, reducing its activity. Thus, in practice, it has not found application as a disinfectant.

The closest in composition and type of action is the aerosol disinfectant "Vilag-2", including, wt.%:

Vilagin 4.0-5.0

Resin SVAP-3 (50% alcohol solution) 0.25-1.5

Propellant 20-50

Ethyl alcohol - the rest

(RF patent No. 2119519, IPC A61L 2/22, С09К 3/30, bull. No. 27, 1998)

However, in view of the resolution of the Ministry of Health of the Russian Federation of the Department of Sanitary and Epidemiological Supervision dated 01.01.2001 “On the prohibition of the production in the Russian Federation of disinfectants based on chlorofluorocarbons”, the drug “Vilag-2” cannot be produced, and therefore it can be used in practice, since it contains 2 Freon (or chlorofluorocarbon) No. 11 and No. 12 in an amount up to 50%, which are azo-hazardous.

Thus, the disadvantage of the prototype is the presence of 50% ozone-depleting chladones, a shorter shelf life, a narrow range of disinfectant effects.

The objective of the invention is the creation of a low-hazard disinfecting composition with a shelf life of at least 26 months with high disinfecting properties on a wide range of pathogenic bacteria, as well as destroying molds that are the causative agents of serious human diseases.

This problem is solved in that a 50% aqueous solution of a vinylpyrrolidone copolymer (hereinafter referred to as SWAP-3 resin) with vinyl acetate, ethanol and a bactericidal additive, 2-methyl-5-di (trifluoromethyl) oxymethylfuran, are included in the following ratio, wt.%:

Vilagin (2-methyl-5-di (trifluoromethyl) oxymethylfuran 6.0-8.0 50% aqueous solution of resin SWAP-3 0.1-1.0 Ethyl technical alcohol rest

Below is a causal relationship of the totality of the essential features of the proposed composition with the achieved technical result.

Table 1 shows examples of analog formulations by action and the proposed funds.

Table 1 Tool Components Vilag-2, wt. % Villa des, wt.% Vilagin 4.0-5.0 6.0-8.0 SWAP-3 (50% alcohol solution) 0.25-1.5 (50% aqueous solution) 0.1-1.0 Ethanol rest rest Propylene 20.0-50.0 -

Distinctive features of the proposed funds are:

1. An unobvious increase in the content of bactericidal additives to 6.0-8.0%.

This made it possible to detect a wider range of disinfecting effects in Ville-dez, including the destructive effect on tuberculosis mycobacteria, pathogenic molds, and reduced the time of destructive effects on viruses.

2. The non-obvious use of a 50% aqueous solution of SVAP-3 reduced the toxicity of the claimed drug and increased the shelf life to 26 months from the date of manufacture (Table 2).

table 2 Name of funds Anti-tuberculosis properties Virucidal properties Fungicidal properties Toxicity LD ₅₀ mg / kg Expiration date (month) Ville des + 100% virucidal effect after 30 minutes. + > 7000.0 26 Vilag-2 - 99.98% virucidal effect after 60 minutes. - 5000.0 12-14

To prevent the rapid evaporation of the bactericide Vilagin from the treated surfaces, a 50% aqueous solution of a copolymer of vinyl pyrralidone with vinyl acetate (hereinafter SVAP-3) in the amount of 0.1-1.0% was introduced.

The introduction of SWAP-3 in the formulation in an amount of less than 0.1% leads to a decrease in the disinfecting effect and an increase in the multiplicity of processing to achieve the same result.

Keeping the resin SVAP-3 more than 1.0% leads to the creation of a film on the surface and does not increase the disinfecting effect.

The optimum in useful properties is the content of SVAP-3 resin in an amount of 0.5%, since the disinfecting effect for all tested test cultures (E. coli, St.aurus, Bac.cerus) is shown to be maximum (99.9-100%) .

An increase in the content of Vilagin in the agent up to 6.0-8.0% made it possible to obtain a 100% disinfecting effect against Mycobacterium tuberculosis, molds.

Due to the simplification of the composition of the disinfectant due to the exclusion of freons in the spectrum of application of the composition, the economy of the method of preparation of the composition, this technical solution is new and has an inventive step.

The components of the disinfectant are affordable, produced by the domestic industry, therefore, the proposed composition is industrially applicable.

The method of preparation means consists in simple mixing of the components in a weight ratio at room temperature (18-20 ° C).

The invention is illustrated by the following examples.

Example 1

A study of the effectiveness of the disinfectant effect of the Vilag-2 and Vil-des preparations on a pure culture of tuberculosis mycobacteria was carried out on model tiled surfaces under protein load conditions.

From a monthly culture of mycobacteria with typical cultural properties, a suspension containing 1 ml of 1 billion microbial cells was prepared. A suspension of mycobacteria was applied to a surface test using a manual sprayer on 2 lines and left at t = 20 ° C for 12 hours in the laboratory room. Each test object of the 1st line was irrigated twice until it was completely moistened from a distance of 20 cm with the preparation Vilag-2 (5% ADV), 2 lines with the drug Wil-des (8% ADV). The control served as test objects, which were irrigated with sterile distilled H ₂ O (3 objects) and 90% ethanol (3 objects) instead of disinfectants. After the exposure time, the remaining liquid from the test surfaces was collected with sterile gauze napkins moistened with sterile distilled water, and each was placed in test tubes with 3.0 ml of 10% sodium phosphate solution for 10 min to neutralize disinfectants. Then they made inoculations from each tube of 0.1 and 0.5 ml per 2 culture media — Levinshtein — Jensen and Mordovsky. 280 samples were investigated. Crops were incubated in an incubator at 37 ° C for 90 days. The growth control of the culture was carried out visually 1 time in 7 days and recorded after 30, 45 and 90 days. The results are presented in table 3. From where it is seen that the complete disinfection of 100% of the test surfaces occurred when using des. means of Ville-des (8% ADV) with a holding time of 60 minutes The drug Vilag-2 (5% ADV) analogue in action even after 60 minutes did not find a sufficient disinfecting effect, its disinfection rate was 99.77.

Thus, the disinfectant Ville-dez exhibits a higher bactericidal effect against tuberculosis mycobacteria.

The results of studies of the anti-tuberculosis activity of the preparations (double treatment; surface-tile; irrigation method) are given in table 3.

Table 3. Name of drugs (% ADV) Holding time (min) The number of colonies in experimental samples % disinfection in relation to water control Number of repetitions Vilag-2 (5% ADV) thirty 53 99.13 twenty Vilag-2 (5% ADV) 60 fourteen 99.77 twenty Ville-des (8% ADV) thirty 24 99.60 twenty Ville-des (8% ADV) 60 0 100.0 twenty Control 1 (water) 60 6110 0 8 Control 2 (alcohol) 60 14058 0 12

Example 2

The “Vid-des” disinfectant has virucidal properties, moreover, with a microbial load, it is stronger than the proven “Vilag-2” means with a lower exposure. So, to assess the virucidal activity of Vid-Dez, we also used type 1 post-myelitis virus (LS vaccine strain with 2 kv) according to the method described in the Guidelines for the determination of virucidal activity of drugs No. 1119-73, approved. Ministry of Health of the USSR.

The initial control titer of the virus in the experiments with “Vilag-2” was 9.7 * 10 ⁴ , and in experiments with “Ville-des” - 10 ^6.35 .

The treatment of infected surface surfaces was performed twice; the inactivated cattle serum served as a protein load.

The results are presented in table 4.

Table 4 Test surface Vilag-2 Ville des Death Time - 100% Defect Linoleum 45 min 30 minutes Oil painted wood 60 min 30 minutes The control 9.7 * 10 ⁴ 1 * 10 ^6.35

Table 4 shows the advantage of the proposed compound, namely, 100% virucidal effect in Ville-dez occurs 1.5-2 times faster than in "Vilag-2".

The main advantage of the new means of "Wil-des" are fungicidal properties that are not available in "Vilag-2".

Example 3

Investigations of the Ville-dez anti-fungal agent were carried out by conventional methods on 3 types of molds: Aspergillus niger, Aspergillus fumigatus and Penicillium chrisogenum and on 3 types of model surfaces: tile, linoleum and painted wood. Microbial load amounted to 2 billion microbial proteins in 1 ml, protein protection - 20% inactivated serum.

Table 5 Types of surfaces Exposition CFU at 72.3 cm ² - the percentage of disinfection Repetition Aspergillus niger Aspergillus fumigatus Penicillium chrisogenum tile 2 (15 '+ 30') 0-100.0 1.3-99.99 0-100.0 3 2 (15 '+ 45') 0-100.0 0-100.0 0-100.0 3 the control 28197 25305 27634 3 linoleum 2 (15 '+ 30') 0-100.0 1,0-99,99 0-100.0 3 2 (15 '+ 45') 0-100.0 0-100.0 0-100.0 3 the control 29308 27123 29197 3 Painted tree 2 (15 '+ 30') 1,5-99,99 1.9-99.99 1,6-99,99 3 2 (15 '+ 45') 0-100.0 0-100.0 0-100.0 3 the control 31455 33981 30757 3

Thus, the tool "Ville-des" has fungicidal properties against mold fungi with double surface treatment and exposure time of 45-60 minutes.

Example 4

Means "Ville-des" has a wide range of disinfectant effects. Thus, studies of the antimicrobial properties of Ville-des and Vilag-2 by irrigation on tiled surfaces infected with 2 ml test cultures of Shigella flexneri (2a527), Proteus vulgaris (5/26), Pseudomonas aerujiuosa (165), Klebsiella pheumonie (444), Salmonella typhymorium (2148), Candida albicaus, Trichophiton rubrum showed 100% effect of the drug "Wil-des" and only 93.0-96.0% disinfectant effect of the drug "Vilag-2" (Table 6) .

Table 6 The results of the study of the disinfecting properties of the preparations "Wil-des" and "Vilag-2" by irrigation. (total exposure - 30 min, microbial load of 2 billion in 1 ml, protein load of 20% inactivated serum). Test surface - tile Name of funds Disinfection rate Shigella flexneri Proteus vulgaris Pseudomonas aerujiuosa Klebsiella pheumonie Salmonella typhymorium Candida albicaus Trichophiton rubrum Ville des 100.0 100.0 100.0 100.0 100.0 100.0 100.0 Vilag-2 95.8 95.6 93.0 96.0 95.8 96.0 95.0

In terms of safety parameters, the claimed “Wil-des” compound, like the analogue according to the action of “Vilag-2”, belongs to the 4th hazard class according to GOST 12.1.007-76, however, the Wil-des is less toxic, because it LD ₅₀ for laboratory animals exceeds 7000 mg / kg. Ville-des does not have local irritant, allergenic, sensitizing, inhalation effects on a living organism.

When studying the physicochemical properties during storage of Ville-des, it was established that all its properties are preserved after 26 months, and the analogue has a shelf life of 12 months by comparison, i.e. the claimed compound has a shelf life of more than 2 times that of the analogue of "Vilag-2".

Thus, the created (disinfecting) preparation, called by the authors "Ville-des", refers to low-hazard substances) is highly effective against bacterial (including tuberculosis mycobacteria), viral infections, and also has disinfectant properties against mold pathogens, has a longer storage period (26 months) and can be used for disinfection of premises, apparatus, devices in hospitals of various profiles, offices, public utilities and on any transport.

Claims (1)

A disinfectant comprising a polymeric organic substance, a bactericidal additive - 2-methyl-5- [di (trifluoromethyl) oxymethyl] furan and ethyl alcohol, characterized in that it contains a 50% aqueous solution of a vinylpyrrolidone-vinyl acetate copolymer as a polymer organic substance in the following ratio of components, wt.%:
2-methyl-5- [di (trifluoromethyl) oxymethyl] furan 6.0-8.0 Vinyl Acetate Vinyl Acetate Copolymer (50% aqueous solution) 0.1-1.0 Technical ethyl alcohol rest