RU2348174C1 - Fodder additive for sucking period piglets - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention is simultaneously relevant for such areas of activities as animal science, veterinary, breeding, plant cultivation, agricultural products processing and biotechnologies; its utilisation for preparation of fodder compounds enables solution of such tasks as microbiocenosis normalisation, gastrointestinal system fermentative processes intensification, carbohydrate metabolism enhancement and prevention of non-contagious diseases which ultimately results in better preservation of swine livestock. For the proposed fodder additive preparation a probiotic ingredient (Lb. Acidophilus lactic acid bacilli isolate in the form of "Biobacton" dietary supplement) is mixed with a vegetative powder preparation of dried stevia leafy mass. The additive is introduced in the diet of sucking period piglets.

EFFECT: normalisation of microbiocenosis related to acidogenous malfunctions; enhanced enzyme activity and digestibility of fodder compounds; carbohydrate metabolism improvement; productivity optimisation; swine livestock sanity and environmental safety.

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Description

FIELD OF THE INVENTION

The invention relates to livestock, veterinary medicine, crop production, processing of agricultural products, biotechnology, and is intended for use in feeding suckling piglets and creating methods for maintaining the livestock of farm animals by introducing probiotic and plant additives into their diet to activate enzymatic, increase the acidogenic activity of the gastrointestinal path, regulation of protein-carbohydrate metabolism at various stages of feeding, acceleration of the adaptation period and improving the ecological status of animals and the environment.

BACKGROUND

Known RF patent No. 2062038, CL. A23K 1/16, 1996. Provides the use of a probiotic liquid preparation of amylovarin (strain BT - 24/88) containing 1 ml of 100 million CFU in a dose of 5-15 ml per head per day.

A known method of obtaining a feed additive, which uses a combination of live cultures of probiotic bacteria Runninococcus, Lb. Acidophilus in the ratio of wt.%: Uterine culture of bacteria - 15-35, adsorbent - 65-85 (RF Patent No. 2190332, Cl. A23K 1 \ 16, 2002), as well as probiotic feed additives containing animal bifidobacteria and streptococci ( Development of the Research Institute of Agriculture of the North-East, Kirov Region) - streptobifid, lacticide - live cultures of lactic acid bacteria and streptococci grown on milk whey (Development of GosNIIIPM).

There is data on a producer of a natural sugar substitute for dietetic nutrition, primarily for patients with diabetes mellitus - Stevia plants (Stevia rebaudiana Bertoni). Biochemical studies of stevia leaves showed that they have a low calorie content and have the property of lowering blood sugar levels (A.A. Krivenko. Prospects for the use of a new sugar-bearing culture (Stevia rebaudiana Bertoni). / A.A. Krivenko, V.I. Zhabina, O. V. Mironova, A. I. Gavrilov, L. D. Ostroukhova. // III international practical conference "State of the biosphere and human health" - Penza, 2003, pp. 91-93).

Known feed additive from the leaf-stem mass of stevia TU 9759-004-00668620-2007.

Known biologically active additive containing up to 10 ⁹ lactobacilli Lb. Acidophilus in the form of dietary supplements Biobacton, which have antagonistic activity against a wide range of pathogenic and conditionally pathogenic microorganisms of the intestine to create favorable conditions for the development of microflora and functions of the digestive tract and metabolic processes.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is "The method of growing piglets", RF Patent No. 2062038, 1996, Cl. A23K 1/16, which provides for the oral administration of a probiotic mixed with feed to piglets individually or in a group manner during the suction period, obtained on the basis of amylovarin (Lb. Amilovorus) BT - 24/88, containing 100 ml CFU in 1 ml, and introduced into the feed at the rate of 5-15 ml per head per day by lubricating the nipples in sows before feeding the newborn.

The disadvantage of this method is that when feeding piglets, only one factor is used that makes it possible to digest food - this is the use of a probiotic amylovarin.

The invention in comparison with the prototype and other known technical solutions has the following advantages: makes it possible to expand the use of probiotic additives containing Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton and a vegetable feed supplement containing a stevioside complex as the active substance, which allows creating a balanced system that activates reflex functions and provides normobiocenosis of animals.

SUMMARY OF THE INVENTION

The objective of the invention is to develop a feed additive for piglets of the suckling period, which could normalize the activity of the gastrointestinal tract by increasing the bifidogenic factor, activate enzymatic activity, carbohydrate metabolism.

The purpose of the invention is the creation of a feed additive for piglets of the suckling period using local probiotics and plant components for the treatment and prevention of non-communicable diseases, normalizing microbiocenosis, activating the enzymatic activity of the gastrointestinal tract, improving carbohydrate metabolism, increasing the gain in live weight, ensuring an increase in the adaptation period of development, increase safety and ensure the improvement of the ecological condition of piglets and the environment of various zones of Stavropolskog edge.

The technical result is achieved using a feed supplement, including probiotics - Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton and a herbal supplement containing a dry powdery leaf-stem mass from stevia, wt.%:

Biobactone 90 Herbal supplement - Dry powder Stevia leaf mass 10

The essence of the invention lies in the fact that for the preparation of a feed additive probiotics - Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton is mixed with a herbal supplement - dry powdered leaf-stem mass from stevia, previously processed by thoroughly grinding and mixing the ingredients in the mixer for 20 minutes.

Tests show that the proposed feed supplement compensates for the deficiency of useful microorganisms in the diets necessary for the growth and development of pigs, which increase the enzymatic activity of the gastrointestinal tract and improve carbohydrate metabolism, which leads to improved productive indicators.

The use of the proposed feed additive for piglets of the suckling period will allow to obtain the effect due to the normalization of microbiocenosis, increased digestibility of feeds by activating the gastrointestinal tract, improving carbohydrate metabolism, which will lead to an increase in live weight, increase preservation and improve the ecological state of piglets and the environment of various zones Stavropol Territory.

DETAILED DESCRIPTION OF THE INVENTION

Examples of specific performance of obtaining a feed additive.

Example No. 1

To prepare 100 kg of a feed additive, mix, wt.%: Biobactone dietary supplement - 95.0 with a herbal supplement - dry powdery leaf-stem mass from stevia - 5.0, pre-processed by thoroughly mixing the ingredients in the mixer for 20 minutes.

Analysis of experimental data on the use of feed additives obtained according to example No. 1 showed an increase in productive indicators: the safety of piglets by 13.7-14.2%, average daily weight gain by 15.7-18.3%, payment of feed by 1.8- 2.3%.

Example No. 2

The feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of the feed additive is mixed, wt.%: Dietary supplement Biobacton, 90.0 with a herbal supplement - dry powdery leaf-stem mass from stevia - 10.0, previously processed analogously to example No. 1.

Analysis of experimental data on the use of feed additives obtained according to example No. 2 showed an increase in productive indicators: the safety of piglets by 23.7-24.8%, average daily weight gain by 25.7-28.3%, payment of feed by 2.3- 2.8%.

Example No. 3

The feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of the feed additive is mixed, wt.%: Biobactone dietary supplement, 85.0 with a herbal supplement - dry powdery leaf-stem mass from stevia - 15.0, previously processed analogously to example No. 1.

The analysis of experimental data on the use of the feed additive obtained according to example No. 3 showed an increase in productive indicators: the safety of piglets by 23.7-24.8%, average daily weight gain by 25.7-28.3%, payment of feed by 2.3-2 ,8%. Control group:

the diet of the control group consists without the use of additives.

The invention in comparison with the prototype and other known technical solutions has the following advantages.

Lactobacillus culture Lb. Acidophilus in the form of dietary supplement Biobactone provides:

- improvement of the enzymatic and acidogenic activity of the gastrointestinal tract, normalization of microbiocenosis.

The presence of a herbal supplement - dry powdered leafy mass from stevia provides:

- regulation of carbohydrate metabolism;

- environmental cleanliness.

In general, the combination of feed additive components provides:

- normalization of microbiocenosis of animals and the environment;

- acceleration of the adaptation period of piglets;

- gain in live weight;

- increase in productive indicators;

- improvement of the ecological state of animals and the environment.

FODDER SUPPLEMENT COMPONENTS FOR PIGLING PIGLING PERIOD

Creation of a feed supplement using environmentally friendly plant materials, biologically active additives - probiotics based on live biocultures of microorganisms to normalize microbiocenosis, enhance the enzymatic activity of the gastrointestinal tract, improve carbohydrate metabolism, increase live weight gain, ensure an increase in the adaptation period of development, increase preservation and improving the ecological condition of farm animals and the environment of various zones of the Stavropol kr i.

BIOLOGICALLY ACTIVE VEGETABLE COMPONENTS - STEVE SHEET MASS

Inadequate intake of various nutrients by the body negatively affects not only animal health, but also human health, which can be the main cause of human diseases. The natural ingredients of dietary supplements not only make up for the deficiency of feed substances, but also have a positive effect on various systems of the animal organism.

Thus, the main purpose of dietary supplements is to provide the body of an animal with nutritional support, disease prevention, assisting and mobilizing the body's defenses to fight diseases, and supporting physiological, biological and environmental health.

The normal development of a living organism is a rather complex process, and a large number of different components and nutrients are needed to maintain it.

For the normal development of the animal organism, biologically active plant components are necessary.

Plant material is a feed containing fiber and lignin, rich in provitamins, vitamins and BEV - biologically active extractive substances necessary to increase the usefulness of animal feeding diets.

Biologically active substances (BAS) are natural or synthetic products that are used in small doses when fed to farm animals and poultry, which have high biological activity. Dietary supplements are necessary to fill the deficit of certain substances vital to the body of the animal, which they receive in insufficient quantities with feed. Most feeds do not currently contain many energetically important substances, vitamins, minerals. Long-term storage, the use of preservatives, the use of chemical fertilizers, the artificial acceleration of ripening, non-technological methods of production and processing are factors that contribute to the disappearance of essential nutrients from feed products, reduce their feed value, quality and safety.

However, herbal supplements used for fattening piglets do not provide complete assimilation of feed.

Cultivated in the Stavropol Territory, a new technical culture - honey grass - Stevia (Stevia Rebaudiana Bertoni) has environmental cleanliness and biological activity, as it contains active biologically active substances - fat, protein, fiber, as well as minerals - calcium, phosphorus, Bev, carotene.

The characteristics and component composition of a new cultivated sugar-bearing industrial crop - honey grass - Stevia (Stevia Rebaudiana Bertoni) - a potential plant component for feed additives are given in Tables 1, 2, 3, 4.

Table 1
Organoleptic indicators of dry leafy mass from stevia The name of indicators Characteristic and norm 1. Appearance A mixture of leaves, stems, buds and flowers with a particle size of from 0.4 to 4 mm 2. The color of the leaf and stems From light green to green. Yellow to brown color allowed 3.Smell Weak, inherent in this raw material, without extraneous odors 4. Taste Sweet, with a bitter taste

Physico-chemical parameters of dry leaf-stem mass from stevia meet the requirements specified in table 2.

table 2 Name of indicator Mass fraction,% ND on test methods Crude protein, not less than 10.0 GOST 13496.4-93 Crude fiber, not more 24.0 GOST 13496.2-91 Crude ash, insoluble in HCl, not less than 11.6 GOST 26226-95 Crude fat, not less 1,02 GOST 13979.2-94 Moisture, not more 10.12 GOST 13496.3-92 Calcium, not less 1,08 GOST 26570-95 Phosphorus, not less 0.87 GOST 26657-97 Bev 43.39 GOST 27548-97 Glycosides 4.4-4.6 TU 10.18 USSR-76-87 Carotene, not less 16.25 GOST 27548-97 Permissible mass fraction of nitrates,% no more 200 GOST 13496.19 The amount of exchange energy, MJ, not less 10.68 GOST 27978 Number of feed units, not less 0.9 GOST 7978

According to the microbiological indicators and the content of bacterial semen and pathogenic microflora according to the “Rules for bacteriological research of feeds”, the dry leafy mass from stevia meets the requirements presented in Table 3.

Table 3 microbiological indicators Norm Bakemsemen, not more than 1 gr 5 · 10 ⁵ Pathogenic microflora Not allowed

In order to use stevia as a source of increasing the ecological status of weakened animals and to develop environmentally friendly biologically active feed additives of a new generation using plant components, studies were conducted to determine the ecological purity of the stevia plant. It was found that in the leaves and stems of the stevia variety Ramonskaya sweetened, cultivated in the Stavropol Territory, the content of lead, arsenic, mercury, cadmium, HCH, DDE, DDT, nitrates, etc. is within the limits allowed by SanPiN for plant-based dietary supplements.

The safety indicators of dry leafy mass from stevia correspond to the requirements presented in table 4.

Table 4 Indicators Allowed Levels ND on test methods Arsenic, mg / kg, not more than 0.5 GOST 9958-81 Mercury, mg \ kg 0.05 GOST 26927 Lead, mg / kg, no more 3.0 GOST 28875-90 Cadmium, mg / kg, no more 0.3 GOST 28875-90 Radionuclides: cesium - 137 200 Bq / kg MU 5779-91 strontium - 90 one hundred Bq / kg MU 5778-91 General toxicity Not allowed GOST R 52 337 Pesticides GOST 13496.20 HCST 0.2 DDT and its metabolites 0.05

The content in the leaves of stevia of manganese, copper and zinc, obviously, determines its therapeutic effect in a number of diseases and as a corrector of immunity. Satisfying the physiological needs of animals in minerals depends on their bioavailability from various feed additives. Vegetable feed consumed by animals only partially satisfies the requirements for some mineral substances, for example, calcium - by 20-30%, phosphorus - by 30-45%. Therefore, for proper feeding, it is necessary to additionally introduce in the diet of animals supplements containing macro- and microelements.

Table 5 shows the microelement composition of the leaves and stems of stevia.

Table 5 The trace element composition of leaves and stems of stevia, mg / kg No. Name Manganese Zinc Copper one. Leaves 14.0 33.8 7.0 2. The stalks 4.0 7.0 4.2

As a result of studies of the microelement composition of leaves and stems of stevia, data were obtained that the leaves of stevia in comparison with the stems contain 3.5 times more manganese, 4.8 times more zinc, almost 2 times copper.

The leaves and stems of stevia contain a complex of biologically active substances that determine the adaptogenic properties of humans and animals. In the complex of modern problems, stevia has properties that are useful for the animal organism, necessary for creating new generation feed additives, and can become a promising culture not only in agronomic practice, but also in animal husbandry.

BIOLOGICALLY ACTIVE ADDITIVES - PROBIOTICS IN THE FORM OF LIVING BIOCULTURES OF MICRO-ORGANISMS

The efficiency of animal feed use depends on a number of factors, including the conditions of keeping and feeding, as well as their genetic potential. To partially replace the missing biologically active substances that stimulate the growth, development and vital activity of the body, as well as normalize the biocenosis, it is necessary to use dietary supplements of microbial origin.

In countries with highly developed livestock breeding, probiotics are widely used to prevent intestinal dysbiosis of young farm animals and birds.

Interest in probiotics as environmentally friendly drugs has intensified the processes of studying the biological properties of bacteria, the most active in probiotic terms. The most studied and implemented is the creation and use of bacterial preparations using microorganisms - probiotics.

Drugs and dietary supplements produced by biotechnological methods using live microorganisms are used to stimulate nonspecific immunity, prevent and treat mixed gastrointestinal infections, and digestive disorders resulting from various factors.

Biologically active substances, probiotics are a new class of drugs that affect the body at a systemic level. Their influence affects regulatory systems, due to which nonspecific resistance of the organism, immunity is activated. The use of probiotics allows immunocorrection of the body.

Acidophilic lactobacilli in the form of dietary supplement "Biobacton" TU 9229-054-07532800-2005 contain up to 10 ⁹ bacterial cells Lb. Acidophilus strain 12 b. This strain of acidophilic lactobacilli is safe: it contains live microorganisms that "colonize" the digestive tract and have the ability to inhibit or inhibit the growth of bacteria that cause gastrointestinal upset. By the mechanism of action, the Biobacton dietary supplement replenishes the deficit of acidophilic rods and has a high antagonistic activity against a wide range of pathogenic and conditionally pathogenic microorganisms, which leads to the displacement of the latter from intestinal microbiocenosis and the creation of favorable conditions for the normalization of microflora and digestive tract function, metabolic processes.

In general, the combination of components of the feed additive for piglets of the suckling period - biologically active additives: probiotics - live cultures of Lactobacillus Lb. Acidophilus strain 12 b and vegetable - leaf-stem mass powder from stevia provides increased reproductive qualities and productive indicators.

Claims (1)

A feed additive for suckling piglets, including a culture of Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton and herbal supplement - dry powdery leaf-stem mass from stevia, wt.%:
BAA Biobacton 90 Herbal supplement - dry powdered leafy mass from stevia 10