RU2233122C1 - Method for predicting psoriasis - Google Patents

Abstract

FIELD: medicine, dermatology.

SUBSTANCE: the present innovation deals with treating skin diseases. For this purpose one should perform microscopic testing of biological tissue, moreover, it is necessary to sample blood serum at the volume of 20-25 mcl to apply it onto the slide's surface, dry it for 18-24 h at 20-30 C, and at detecting marginal-amorphous area at the surface of dried drop along with intermediate area - with single, disseminated bracket-shaped structures and central one - with rounded structures of irregular shape and with cavities and hollows located at the background of amorphous central area it is possible to diagnose psoriasis. The present innovation enables to perform diagnostics of psoriasis at short period of time at the lowest labor expenses and, moreover, it is atraumatic.

EFFECT: higher efficiency of diagnostics.

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Description

The invention relates to medicine, namely to laboratory methods for the study of skin diseases, and can be used in the diagnosis of psoriasis.

Known laboratory methods for the diagnosis of psoriasis according to the immunomorphological picture (L.V. Beletskaya et al. Labeled antibodies in normal and pathological morphology (atlas), M., 2000, p. 19).

The clinical picture of psoriasis, which the doctor observes when examining the patient, is characterized by a monomorphic rash in the form of flat pink-red papules up to 1 cm in size, a dense consistency, slightly rising above the level of healthy skin. The following diagnostic phenomena are characteristic of these rashes - the psoriatic triad (the phenomenon of stearin stain, the phenomenon of psoriatic film, the phenomenon of blood dew).

The disadvantage of this method is the low reliability of the diagnosis, since it is based on the subjective assessment of a doctor.

The closest is a method for the diagnosis of psoriasis, including a microscopic examination of biological tissue (B.A. Berenbein and others. Differential diagnosis of skin diseases. - M., Medicine, 1989, p. 106.

In this method, the diagnosis of psoriasis is carried out by histological examination of a skin sample. In this case, characteristic morphological changes on the surface of the skin are determined: parakeratosis, acanthosis, thinning of the malpigium layer over the elongated papilla of the dermis. Lymphomacrophagic infiltration around the vessels of the dermis is characteristic.

The disadvantage of this method is its invasiveness, complexity, this study requires a significant investment of time and the use of expensive equipment.

The task was to eliminate these shortcomings and create a more economical and non-traumatic method that would allow in a short time to diagnose psoriasis with the least labor.

The problem is solved in that in a method for the diagnosis of psoriasis, including microscopic examination of biological tissue, a blood serum is taken in a volume of 20-25 μl, applied to the surface of a glass slide in the form of a drop, dried for 18-24 hours at a temperature of 20-30 ° C , and upon detection on the surface of the dried drop, the marginal - amorphous zone, the intermediate - with single, scattered bracket-like structures and the central - with rounded structures of irregular shape with voids and recesses placed on the background of the amorphous surface of the cent zone, diagnosed with psoriasis.

Figure 1 shows the appearance of a dried drop with skin lesions of a non-psoriatic nature (eczema); figure 2 is the same in the presence of psoriasis.

The method is as follows.

Blood is taken from a patient in a test tube, kept for 2 hours at a temperature of 20-30 ° C. Then a blood serum sample is applied to the surface of the slide in an amount of 20-25 μl, applied to the surface of the slide in the form of a drop and left at a temperature of 20-30 ° C until completely dried. Then, under the small magnification of the microscope (× 15), they look at the surface of the entire droplet and, when the dried droplet is found on the surface, the marginal — amorphous zone, the intermediate — with single, scattered bracket-like structures and central — with irregular-shaped round structures with voids and recesses placed against the background amorphous surface of the central zone, psoriasis is diagnosed.

Examples.

1. Patient S., born in 1955, medical history No. 1321. The diagnosis at admission is exacerbation of chronic eczema. A 25 μl blood sample was taken and applied as a drop to the surface of a glass slide. The dried drop has different background color, clear and abundant structuring, located both in the central zone of the drop and in the transition and peripheral zones, with an abundance of cracks, nuclei, nuclear formations, waves of chemical activity. The presence of pathological structures in the form of Arnold languages in the transition and marginal zones was also detected (Fig. 1).

Conclusion: non-psoriatic skin lesions.

2. Patient P., born in 1953, medical history No. 13804. The diagnosis at admission is chronic eczema. Microscopy on the surface of the dried droplet revealed the following: edge - amorphous zone, intermediate - with single, scattered bracket-like structures and central - with rounded structures of irregular shape with voids and recesses similar to “geographical structures”, which are placed against the amorphous surface of the central zone (Fig. .2).

Conclusion: common vulgar psoriasis.

The method is technically simple. With its help, you can diagnose psoriasis with an unclear clinical picture without histological and histochemical studies.

Claims (1)

A method for the diagnosis of psoriasis, including microscopic examination of biological tissue, characterized in that a blood serum is taken in a volume of 20-25 μl, applied to the surface of a glass slide in the form of a drop, dried for 18-24 hours at a temperature of 20-30 ° C and at revealing on the surface of a dried drop an edge - amorphous zone, an intermediate - with single scattered bracket-like structures and a central one - with round structures of irregular shape with voids and grooves placed centrally against the amorphous surface zone diagnose psoriasis.

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