RU2279874C9 - Preparation for prophylaxis and treatment of horse with helminthism - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention proposes a preparation that comprises compound among avermectins group, praziquantel or phenazal and special additives in the following ratio of components, wt.-%: compound among avermectins group, 0.05-10.0; praziquantel or phenazal, 3.0-70.0, and special additives, the balance. Enhances resistance of horses against the repeated infection.

EFFECT: valuable veterinary properties of preparation.

2 cl, 6 ex

Description

The invention relates to the field of veterinary medicine and can be used for the prevention and treatment of horse helminthiases.

Known antiparasitic drugs for the treatment and prevention of helminthiasis in horses:

Emboteip (Baimida, Ireland) - as DV contains 400 mg of pyrantel embonate.

The disadvantage of this drug is that in horses with helminthiases, the drug is used in different doses - with strongilatoses, oxyurosis, cyatostominoses and parascaridosis at a dose of 19 mg per kg of animal weight, with anoplocephalidoses - 38 mg of embonate pyrantel per kg of weight (see, for example, Klenova I.O., Maltsev K.L., Yaremenko N.A., Arkhipov I.A. Veterinary preparations in Russia, M., Selkhozizdat, 2004, v. 1, p. -302, 393).

Panakur paste (Intervet, Holland) - DV fenbendazole, is prescribed at a dose of 7.5 mg / kg.

The disadvantage of this drug is that horses develop resistance to fenbendazole, as an anthelmintic. To ivermectin, it occurs mainly in sheep and goats (see, for example, Viktorov A.V., Drinyaev V.A. Development of resistance to ivermectin // Veterinary Medicine. 2002. No. 4, Andrews et el. Cbemotberapy of Parasitic Diseases London, New York, 1986. P.-498).

Equisect paste (Farmbiomed, Russia) - DV aversectin C - composition of a natural avermectin complex obtained by microbiological synthesis using Streptomyces avennitilis culture. The drug is given once inside at a rate of 0.23 mg / kg body weight (2 g of paste per 10 kg of animal body weight) (see, for example, Klenova I.F., Maltsev K.L., Yaremenko N.A., Arkhipov I.A. Veterinary preparations in Russia, M., Selkhozizdat, 2004, vol. 1, p.302, 393).

The disadvantage of this drug is that on a horse weighing 500-600 kg, 100-120 g of the drug must be set.

The closest is Equalan (Merial, France), a paste containing 1.87% ivermectin (see, for example (WO 01/60409 A, 08/23/2001). The disadvantage of this paste is that Equalan has no effect on cestode.

The objective of the present invention is to develop a highly effective drug for the prevention and treatment of horse helminthiases, including nematodoses and cestodoses, which, when reducing the dose of active substances, can expand the spectrum of action of the drug and increase the resistance of animals to re-infection.

The problem is solved due to the fact that the preparation for the prevention and treatment of animal helminthiasis, containing a compound from the group of avermectins and target additives, according to the invention additionally contains praziquantel or phenasal in the following ratio of ingredients, in wt.%:

compound from the group of avermectins 0.05-10.0 praziquantel or phenasal 3.0-70.0 targeted supplements rest

As a compound from the group of avermectins, the drug contains ivermectin, or abamectin, or doramectin, or moxidectin.

The mechanism of action of a compound from the group of avermectins in nematodes is to stimulate the formation of gamma-aminobutyric acid (GABA) by nerve endings with increased binding of GABA to polysympathetic GABA receptors. In this case, the transmission of nerve impulses is blocked, causing nematode paralysis.

The derivative of prazinoisoquinol is praziquantel (molecular formula - C ₁₉ H ₂₄ N ₂ O ₂ ), the mechanism of action of which is based on vacuolization of tegument and muscle contracture of helminths (see, for example, Andrews et el. Cbemotberapy of Parasitic Diseases, London, New York, 1986 , p. 498).

Phenasal is effective only in animal cestodoses. The disadvantage of phenasal is that it is effective only at high therapeutic doses, and also that the drug has a side effect - it can cause vomiting or salivation in animals (see, for example, RU 2123332 C1, 12.20.1998).

As targeted additives, the preparation contains a solvent (e.g., distilled water), a solubilizer (e.g., sorbitol or polyvinyl-pyrrolidone), a preservative (e.g., sugar or Santoquin), and an emulsifier (e.g., gelatin).

The technical result provided by the invention is to increase the effectiveness of the drug by expanding the spectrum of its action while reducing the concentration of compounds from the group of avermectins and praziquantel or phenasal, as well as increasing the resistance of horses to reinfestation.

The preparation according to the invention is illustrated by the following examples, which however do not cover, and moreover do not limit the entire scope of the claims of this invention.

Example 1

The drug is prepared as follows.

To the compound from the group of avermectins, praziquantel, emulsifier, preservative, solubilizer and distilled water are gradually added. The mixture is passed through a dispersant to obtain a homogeneous mass.

Get the drug in the form of a paste of the following composition (in wt.%):

- Ivermectin 2.0 - Praziquantel 10.0 - Sugar 20,0 - Santokhin 0.02 - Sorbitol 5,0 - Polyvinylpyrrolidone 10.0 Gelatin 4.0 Distilled water - rest

Example 2

Similar to the first example, only the drug contains abamectin and phenasal (in wt.%):

Abamectin 2.0 Fenasal 10.0 - Sugar 20,0 - Santokhin 0.02 - Sorbitol 5,0 - Polyvinylpyrrolidone 10.0 Gelatin 4.0 Distilled water - rest

Examples characterizing the anthelmintic effectiveness of the drug.

Example 3

In the experiment, horses of the Trakhenin riding breed, Oryol and Russian trotters invaded by Parascaris equorum, Anoplocephala magna and intestinal strongylases of the Strongylidae family, ZAO Moscow Stud Farm No. 1 of the Odintsovo district of the Moscow Region, in the amount of 114 goals (50 heads of young animals and 64 heads of mares) were used ) The animals were given the paste obtained according to example 1, inside, at the rate of 1.0 g of paste per 100 kg of body weight, which amounted to 0.15 mg / kg for ivermectin and 0.8 mg / kg for praziquantel. The effectiveness of the drug against parascaridosis, anoplocephaliasis and strongilata of the digestive canal of horses was 100%.

Example 4

The paste obtained according to example 2 was used for parascaridosis, oxyurosis, strongylatosis of the digestive tract and anoplocephalidosis. There were 54 in one experiment and 17 horses in another. The paste was given individually at a dose of 1.0 g per 100 kg of animal weight, which amounted to 0.15 mg / kg for abamectin and 0.8 mg / kg for phenasal. 100% efficacy against all parasites was obtained.

Example 5

The drug was administered individually, once to 42 horses with rhinestosis and gastrophilia. The effectiveness in rhinestrosis was 99.3-100%, and in case of gastrostylosis 99.9-100%. Three horses receiving three times higher doses of paste had no visible clinical changes.

Example 6

In the experiment on 42 horses, paste was applied to 12 foals, one year old, and 30 heads of mares spontaneously infected with parascaris, strongilates, and anoplocephalids inside, based on DV ivermectin 0.15 mg / kg and praziquantel 0.8 mg / kg, based on 1.0 g per 100 body weight. The intensity of invasion (AI) by strongilates ranged from 48 to 1320 eggs per g of feces, in 9 animals infected with anoplocephalates, from 22 to 110 eggs per g of feces, in 10 animals infected with parascaris, AI ranged from 44 to 1100 eggs per g of feces. Animals tolerated the drug well, deviations in general condition were not noted. The effectiveness of the drug was 100% for nematodoses and cestodoses.

In the study after 8 months of the experimental group, foals and mares of invasion by the above types of nematodes and cestodes were not detected. In the control group (18 goals) of adipinate deworming with piperazine at a dose of 10.0 per 50 kg of live weight with moistened barley for two consecutive days, 8 months later, using the coprological method for studying feces by the Fulleborn method, 2 heads of delafondia and in 8 heads, eggs have anoplocephalus.

The results obtained indicate a high anthelmintic efficacy of the paste in therapeutic doses according to coprolaroscopic studies.

Thus, a highly effective preparation for the prevention and treatment of horse helminthiases, including nematodoses and cestodoses, was obtained, which allows, with a decrease in the dose of active substances, to expand its spectrum of action and increase the resistance of animals to reinfestation.

Claims (2)

1. A drug for the prevention and treatment of horse helminthiasis, containing a compound from the group of avermectins and targeted additives, characterized in that it additionally contains praziquantel or phenasal in the following ratio, wt.%: Avermectin compound 0.05-10.0 Praziquantel or Phenasal 3.0-70.0 Targeted Supplements Rest 2. The drug for the prevention and treatment of horse helminthiasis according to claim 1, characterized in that as a compound from the group of avermectins contains ivermectin, or abamectin, or doramectin, or moxidectin.