RU2269938C1 - Device for topical electron microscopic detection of l -shaped bacteria at nose polypi - Google Patents

Abstract

FIELD: medical equipment.

SUBSTANCE: device can be used in microbiology. It characterizes by peculiarities of taking microorganisms from surface of nose polipi for direct electron microscope testing. Device has rod with cap. Rod is made of iron. Two-sided sticky carbonaceous band is fixed onto cap of rod. Top sticky surface of band is brought into contact with mucous of polipi of nose for getting preparation-print of micro content followed by viewing it in scanning electron microscope. Device doesn't cause traumatism of patient's mucous and allows determining L-shaped bacteria quickly as well as their sizes, shape and types of reproduction onto polyp tissue.

EFFECT: improved efficiency of detection.

3 dwg, 2 ex

Description

The proposed device relates to medical equipment, more specifically to devices used in microbiology, and relates to the technical features of the collection of microorganisms from the surface of the nasal polyps for their direct electron microscopic examination.

A device is known for determining surface contamination by microorganisms in the form of gauze swabs: the first is moistened with a 5% solution of polyvinyl alcohol, the second is moistened with a 0.9% aqueous solution of sodium chloride, and the third is a dry gauze swab. After sequentially wiping the nasal mucosa with tampons, the tampons are washed from microorganisms in one vessel with a 0.9% aqueous solution of sodium chloride, followed by determination of the number of microorganisms in the wash [1]. Such a device has the following disadvantages:

1. The impossibility of direct electron microscopic examination of the material taken with gauze swabs.

2. The inability to detect L-forms of bacteria.

3. Mechanical damage to L-forms.

4. The inability to determine the ratio of microbial cells in their habitat and methods of their division on the surface of the nasal mucosa.

5. A mixture of microorganisms from different topical surfaces of the nasal cavity.

6. The impossibility of taking material from inaccessible and small in surface area of the nose polyp.

7. The inability to pre-select nutrient media for subsequent bacteriological studies.

8. The bulkiness of taking the material, the risk of contamination of gauze swabs with airborne microorganisms.

9. Large expenditures of time, alcohol, gauze, nutrient media.

The aim of the invention is to increase the efficiency of topical electron microscopic detection of L-forms of bacteria, accelerating and simplifying the research process.

The device is prepared in advance for reusable use and sterilized in one of the cold ways.

The device (Fig. 1) consists of a rod 1 made, for example, of iron having a hat 2. On the end surface of the hat 2 of the rod 1 is fixed carbon double-sided adhesive tape 3 having one adhesive side 4 and the other adhesive side 5 with a protective coating 6 made, for example, from waxed paper. One of its sticky side 4 is glued to the hat, its other sticky side 5 with a coating 6, which protects the adhesive surface from drying and contamination, is facing outward.

The device is used as follows.

In a sterile glass Petri dish with adhesive tape 3, its adhesive side 5, the protective coating 6 is removed. A sterile nasal mirror is inserted into the patient’s nasal cavity, the device is gripped by the rod 1 with tweezers and the adhesive side 5 of the adhesive tape 3 is pressed against the surface of the polyp. As a result, on the sticky side 5 of the adhesive tape 3, an imprint of the L-form bacteria is obtained. After drying in a sterile glass Petri dish, the imprint preparation is sprayed with carbon in a VUP-4 vacuum chamber to create a conductive coating, then the imprint preparation is viewed in a LEO 1420 scanning electron microscope, choosing the optimal magnification from 1500 to 30000 or more times.

The fundamental difference between the proposed device and the known one is that with its help it is possible to detect L-forms of bacteria on a specific surface of the nose polyp without damaging the L-forms of bacteria, accelerating and simplifying the research process.

The proposed device was tested in 10 adult patients with polyposis rhinosinusitis - all on the surface of the polypous tissue were found various variants of L-forms of bacteria.

Examples of the use of the device in a clinical setting.

Patient A., 48 years old. Diagnosis: polypous rhinosinusitis. Using the proposed device, an imprint was taken from the polyp mucosa. The results of viewing the imprint preparation in a scanning electron microscope are presented in FIG. 2: a — fragmentation of the filamentous form and budding of elementary bodies on its surface (enlarged. × 8000); b - the exit of elementary bodies to the surface of the thread along the right contour (increased. × 25000); c - conglomerate from L-forms (enlarged. × 8000).

Patient V., 56 years old. Diagnosis: bronchial asthma, polypous rhinosinusitis. Using the proposed device, an imprint was taken from the polyp mucosa. The results of viewing the imprinted preparation in a scanning electron microscope are presented in Fig. 3: a - fragmentation of the lower sections of the filiform form with budding of elementary bodies along the right contour (increased 9000); b - budding of elementary bodies at the upper pole of the thread (enlarged. × 15000); (d) conglomerates from L-forms (enlarged × 10,000; enlarged × 7,000).

Information sources

1. USSR author's certificate No. 919354, cl. C 12 G 1/00, 1980.

Claims (1)

A device for detecting bacteria, containing a rod with a cap for insertion into the nasal cavity and a double-sided carbon tape to obtain an imprint of the L-forms of bacteria and viewed in a scanning electron microscope, while one side of the adhesive tape is glued to the cap and the other side designed to be pressed to the surface of the polyp, it has a coating to protect the adhesive surface from drying out and contamination.

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