RU2117273C1 - Medium for histological slice inclusion - Google Patents

Abstract

FIELD: medicine, biology, histology. SUBSTANCE: medium consists of 19-21% foam plastic solution in xylene stabilized by addition of dimethylphthalate at ratio 1: 100. Solution drop is applied on slice and dried and slice is inclosed into clear and stable at storage material as a film. Their can be stored in simple paper package without sun rays effect for indefinitely long time. Solution can be prepared easily and stored for a long time without alternations under usual conditions. EFFECT: improved and simplified method, decreased cost.

Description

 The invention relates to medicine and biology, and specifically to methods for the conclusion and storage of histological preparations.

 The purpose of the invention is the replacement of expensive and practically inaccessible synthetic resins for the conclusion of stained sections of tissues and their long-term storage, followed by quick processing for re-examination.

 The goal is achieved by using for the conclusion of histological sections a widespread view of one of the polymers - a solution of polystyrene foam in xylene, which after drying forms a transparent film, is easily separated from the slide, which eliminates the need for coverslips. The resulting preparations can be stored for a long time in paper bags in a dark place, and the main dye does not fade. If it is necessary to re-examine the slice, the foam film quickly dissolves in xylene, the freed slice can be glued to a glass slide.

 The most common medium used in domestic practice for the conclusion and storage of stained histological preparations is Canadian or fir balms, which are solutions of natural resins in xylene. At the same time, during drying due to the volatilization of the solvent, air bubbles are often sucked under the coverslip, which greatly complicates the study of the drug. Often, the resin from the edges falls on the upper surface of the coverslip and, drying up, forms irregularities, which also interferes with the observation under the microscope. With prolonged storage (more than a year), the balms take a yellow color, drying out, cracking, thereby destroying the drug. The latter phenomenon is more often observed during the conclusion and storage of thick sections. In addition, the main aniline dyes in such preparations are discolored, and Prussian blue and eosin fade quite quickly. To avoid these drawbacks, various synthetic resins have been proposed (e.g., cellulose tricaprate, plasticized maleic polymer, cycloparaffin polymer, polystyrene). Most of them are neutral, the basic aniline dyes are well preserved in them, the refractive indices of light for them satisfy the objectives of microscopy, but at the same time they are very expensive and practically inaccessible. To store glass with slices enclosed in resin, it is necessary to have either special boxes or bulky devices in the form of cabinets with drawers.

 Working is a solution of foam in xylene. For microscopy, the best concentration is in the range of 19-21% (with a higher or lower concentration, the clarity of the image in the microscope is significantly reduced). To prepare 300 ml of a solution, 60 g of dry and clean pieces of foam, used as packaging material, are dissolved in 300 ml of o-xylene. To slow down the drying process to prevent wrinkling of the slice after complete dissolution of the foam, a universal stabilizer is added - dimethyl phthalate at the rate of 1: 100 (in this case 3 ml). The resulting clear and colorless solution can be stored indefinitely in bottles with a ground lid under normal conditions without direct sunlight.

 The conclusion of the slices is as follows. After staining and dehydration, a drop of a solution for soaking is applied to the slide with a slice. After 40-60 minutes, the solution is again applied in such an amount that it, spreading out, extends 2.0-2.5 cm beyond the edges of the cut. The solution completely dries out within 20-24 hours, after which the cut can be examined under a microscope. If necessary, the film with a slice can easily be separated from the glass slide, at its edges you can make inscriptions or symbols. Painted sections enclosed in foam film can be stored in plain paper envelopes. On control preparations, even after one and a half years of fading or damage to the sections was not observed. If it is necessary to re-examine the film, it can be easily dissolved in xylene, and the freed cut should be glued onto a glass slide.

Claims (1)

 The environment for the conclusion of histological sections based on resin in xylene, characterized in that the resin contains a 19 - 21% solution of foam in xylene, stabilized with dimethyl phthalate at a rate of 1: 100.