RU2159434C1 - Method for determining the nature of intra-articular inflammation - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves studying optical structure properties of the synovial fluid using polarization photometry method. The sample under study is placed into 0.9% sodium chloride solution as salt medium. Absorption index is determined in optical units. The absorption index value being less than 0.06 optical units, acute inflammation phase is considered to be the case. EFFECT: simplified method. 1 tbl

Description

 The invention relates to medicine, namely to clinical laboratory diagnostics.

Assessment of the condition of the synovial fluid is an extremely important point in the differential diagnosis of injuries and diseases of the joints. Traditionally, the study of joint fluid includes a description of physical properties (color, character, viscosity, and others), counting the number of leukocytes, the content of which, as a rule, correlates with the activity of the inflammatory process, cytological examination (Laboratory research methods in the clinic: Reference book / Menshikov V.V. , Delektorskaya LN, Zolotnitskaya RP, and others; Edited by VV Menshikov. - M .: Medicine, 1987. - 368 p.), Allowing to determine the nature of the exudate - neutrophilic or lymphocytic, which is one of major prize nak differentiation of acute and chronic inflammatory process. In addition, to assess the phase of joint inflammation (acute, chronic), the determination of the concentration of biochemical markers of inflammation, acute phase proteins (acidic α-glycoprotein, α ₁ -antitrypsin, C-reactive protein, haptoglobin, ceruloplasmin, immunoglobulins, etc., is also often used. )

 However, the above methods are either cumbersome and require expensive reagents and highly skilled performer, or subjective and uninformative.

 A known method for assessing intra-articular inflammation by determining the concentration of haptoglobin in the synovial fluid (Udovitsyna TI Methods for the determination of individual proteins. Krasnoyarsk, 1991. - 15 p.), With an increase in which an island-inflammatory reaction in the joint is noted. Its normal content is 0.5-1.4 g / l. The disadvantages of this method are the need to use a large number of a number of reagents and the additional risk of infection of the researcher with infectious diseases in the preparation of one of the reagents. In addition, to obtain a result by this method, a large amount of the studied material is necessary.

 The task is to simplify the assessment of the nature of intra-articular inflammation and reduce research costs is solved as follows.

 In the method for assessing the nature of intra-articular inflammation by examining a synovial fluid according to the invention, the structural and optical properties of a fluid sample in polarized light, placed in a salt medium — 0.9% sodium chloride solution — are examined, the absorption index is determined, and when its value is less than 0.060 optical units conclude about the "island-inflammatory" nature of intraarticular inflammation.

 The method is as follows. The patient's joint is punctured in one of the generally accepted methods. Material for research is taken from the middle part of the supernatant after centrifugation of the synovial fluid. The preparation of an optical cell for investigation by polarization photometry is carried out according to the generally accepted methodology (Zapetskiy E.V. Phase-structural analysis of bile and its importance in the diagnosis of cholecystitis. - Sverdlovsk, 1991): a drop of the test liquid is placed on a glass slide, a drop of isotonic sodium chloride solution is added and carefully close with a coverslip. The absorption index is determined on an SF-16 spectrophotometer, the measurement result is expressed in optical units.

 When the absorption coefficient is less than 0.06 opt. units conclude the acute phase of inflammation, with a coefficient value of more than 0.06 opt. units - about the chronic phase of inflammation.

 Compared with the prototype, the proposed method is more economical, since it does not require special reagents, time-consuming, to obtain the result, it is necessary 50 times less than the studied material. In addition, it is so simple that it can be performed by a laboratory assistant. The advantage of this method is also the fact that without additional costs it allows you to quickly resolve the issue or confirm the result obtained with the Rivalt test on the affiliation of articular effusion to transudates or exudates. The method is quite accurate, which is confirmed by a comparative assessment with the analogue and prototype (see table).

Claims (1)

 A method for assessing the nature of intra-articular inflammation by examining a synovial fluid, characterized in that the absorption rate of a sample of synovial fluid placed in a salt medium is determined - 0.9% sodium chloride solution in polarized light, and when its value is less than 0.06 opt. units ascertain the acute phase of joint inflammation.

Images