RU2149648C1 - Method for producing bandage material - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves oxidizing medical gauze to dialdehyde cellulose with following trypsin immobilization, washing the produced matrix with distilled water, drying and treating with aqueous solution of a biologically active substance like Mexydol. Treatment is carried out with 0.4-0.6% aqueous solution of Mexydol containing 1.8-2.2 mg of Mexydol per 1 cm² of treated material. EFFECT: enhanced effectiveness in treating trophic ulcers. 1 tbl

Description

 The invention relates to medicine, specifically to obtaining dressings with antiseptic properties, for the treatment of mainly trophic ulcers and purulent-septic complications.

 There is a method of obtaining traditional dressings for the treatment of trophic ulcers, consisting in the fact that zinc-gelatin paste is applied to the gauze substrate. Dressings are applied for 30-40 days, sometimes up to 3 months. It is necessary to carry out toilet ulcers with irrigation with proteolytic enzymes and the parallel appointment of physiotherapy (Big Medical Encyclopedia. M: Soviet Encyclopedia, 1985, v.25, p. 347-348). Treatment with traditional dressings is long, protracted and does not always lead to ulcer closure.

 Known closest to the claimed method for producing dressings, consisting in the fact that medical gauze is pre-oxidized with a sodium periodate solution to dialdehyde cellulose, then treated with a trypsin solution, washed and dried. Then the resulting material is impregnated with a 0.02% solution of furatsilina in distilled water (a.c.SSSR N 1474917, class A 61 K 31/25, 1984).

 The resulting material has healing properties and its use leads to a reduction in the treatment of trophic ulcers by an average of 24%. The lack of material is not high enough in the treatment of trophic ulcers.

 The invention solves the problem of expanding the range of dressings and obtaining dressings with high efficiency in the treatment of trophic ulcers.

The problem is solved due to the fact that in the method for producing dressings, including the oxidation of medical gauze to dialdehyde cellulose followed by immobilization of trypsin, washing the resulting matrix with distilled water, drying and treating with an aqueous solution of a biologically active substance, Mexidol is used as a biologically active substance, and processing conduct 0.4 - 0.6% aqueous solution of Mexidol to the content of Mexidol 1.8-2.2 mg per 1 cm ^{2 of the} processed material.

 Mexidol is a derivative of 3-hydroxypyridine and is 2-ethyl-6-methylpyridine succinate - a synthetic antioxidant (P.I. Tolstoy, V.T. Krivikhin et al. Laser radiation and antioxidants in the treatment of purulent-necrotic processes of the lower extremities in patients with sugar diabetes. M: Orbit-M, 1998, p. 43).

Mexidol is a structural analogue of the group of vitamins B ₆
The method is as follows:
First carry out the oxidation of medical gauze 0,065 N. sodium periodate solution at pH 5.5 and room temperature in the dark for 18-20 hours with processing module 5.

 The oxidized material is washed with distilled water and dried at room temperature. The gauze oxidation state is 2%.

 Trypsin immobilization is carried out by treating the matrix with a trypsin solution with a concentration of 0.05% in phosphate buffer at pH 7.0 for 2 hours with processing module 5.

 Then the material is squeezed and dried in air.

A 0.4-0.6% aqueous solution of Mexidol is prepared and an activated matrix containing immobilized trypsin is treated with a module of 4-6 for 12 hours at room temperature until the Mexidol content is 1.8-2.2 mg per 1 cm ² , after which the material is squeezed and dried in air.

 The invention is illustrated by examples.

 Example 1

 Medical gauze is treated with 0.0625 N. sodium periodate solution (pH 5.5) for 20 hours at room temperature in the dark.

Next, the sample is washed with water (5 times for 30 minutes), squeezed to a weight gain of 100% and treated with a 0.05% solution of trypsin in 0.06 M phosphate buffer at pH 7.5 and 4 ^o C for 12 hours with module 5 .

 The obtained sample is washed from a protein that is not chemically bound to the carrier, physiological saline, distilled water and dried in air.

After drying, gauze containing 0.2% chemically bound trypsin is treated with module 5 with a 0.4% solution of mexidol in water to a mexidol content of 1.8 g / cm ^{2 of the} processed material and dried in air. The healing properties of the material are presented in the table.

 Example 2

 The oxidation of medical gauze and the immobilization of trypsin is carried out according to example 1.

Then the material is treated with a 0.5% aqueous solution of Mexidol with module 6 to a Mexidol content of 1.8-2.2 mg / cm ^{2 of the} processed material, squeezed and dried in air. The healing properties of the material are presented in the table.

 Example 3

 The oxidation of medical gauze and the immobilization of trypsin is carried out according to example 1.

Then the material is treated with a 0.6% aqueous solution of Mexidol with module 6 to a Mexidol content of 2.2 g / cm ^{2 of the} processed material, squeezed and dried in air. The healing properties of the material are presented in the table.

 To illustrate the high efficiency of the medicinal properties of the proposed dressing in the treatment of trophic ulcers, trophic ulcers of the lower extremities were treated using traditional dressings, napkins containing immobilized trypsin, and furatsilin and napkins obtained by the proposed method.

Studies were conducted in patients whose ulcerous surface area ranged from 10 to 20 cm ² and from 20 to 40 cm ²
In the first group of patients (treatment using traditional dressings) after cleansing the ulcer surface of purulent and necrotic masses and at the beginning of epithelialization, a zinc-gel dressing was applied, which included an equal amount of zinc oxide and gelatin - 50 g, 300 g of glycerol and 100 ml distilled water.

 In the second group of patients, after the toilet of the ulcer, napkins containing immobilized trypsin and furatsilin were applied.

 The third group of patients was treated with wipes containing immobilized trypsin and mexidol.

 The treatment results are shown in the table.

 An essential step in the treatment of patients with ulcers of the lower extremities is the halving of the ulcer area, which in the first group of patients treated with traditional dressings occurred on 20.4 ± 1.0 days. In the second group of patients treated with Dalcex-trypsin + furatsilin wipes, a 50% reduction in ulcer area occurred by 18.5 ± 1.5 days, and in the third group of patients treated with Dalcex-trypsin + mexidol wipes, the reduction in area ulcers occurred on 15 ± 1.5 days.

 As can be seen from the above data, the use of Dalpex-trypsin + Mexidol wipes shortens the treatment time for trophic ulcers and in most cases leads to ulcer closure.

Claims (1)

A method for producing a dressing material, including oxidizing medical gauze to dialdehyde cellulose followed by immobilization of trypsin, washing the resulting matrix with distilled water, drying and treating with an aqueous solution of a biologically active substance, characterized in that Mexidol is used as a biologically active substance, and the processing is carried out 0.4 - 0.6% solution of Mexidol to a content of 1.8 - 2.2 mg per 1 cm ^{2 of the} processed material.

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