RU2019118791A - Популяции клеток почек и их применение - Google Patents
Популяции клеток почек и их применение Download PDFInfo
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- RU2019118791A RU2019118791A RU2019118791A RU2019118791A RU2019118791A RU 2019118791 A RU2019118791 A RU 2019118791A RU 2019118791 A RU2019118791 A RU 2019118791A RU 2019118791 A RU2019118791 A RU 2019118791A RU 2019118791 A RU2019118791 A RU 2019118791A
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- cell population
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- 210000003734 kidney Anatomy 0.000 title claims 4
- 210000004027 cell Anatomy 0.000 claims 15
- 102000011782 Keratins Human genes 0.000 claims 4
- 108010076876 Keratins Proteins 0.000 claims 4
- 102100033366 Glutathione hydrolase 1 proenzyme Human genes 0.000 claims 3
- 101710205562 Glutathione hydrolase 1 proenzyme Proteins 0.000 claims 3
- 102000011899 Aquaporin 2 Human genes 0.000 claims 2
- 108010036221 Aquaporin 2 Proteins 0.000 claims 2
- 102100034459 Hepatitis A virus cellular receptor 1 Human genes 0.000 claims 2
- 101710185991 Hepatitis A virus cellular receptor 1 homolog Proteins 0.000 claims 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 2
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 2
- 101000998011 Homo sapiens Keratin, type I cytoskeletal 19 Proteins 0.000 claims 1
- 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 210000003292 kidney cell Anatomy 0.000 claims 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/22—Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4741—Keratin; Cytokeratin
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
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- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02002—Gamma-glutamyltransferase (2.3.2.2)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
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- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4742—Keratin; Cytokeratin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
- G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
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- Bioinformatics & Cheminformatics (AREA)
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- Urology & Nephrology (AREA)
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- General Engineering & Computer Science (AREA)
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- Animal Behavior & Ethology (AREA)
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- Developmental Biology & Embryology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
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Claims (13)
1. Обогащенная гетерогенная популяция клеток почек млекопитающих, полученная из образца почки млекопитающего,
где указанная обогащенная популяция содержит клетки, которые экспрессируют VEGF и KIM-1 до детектируемых уровней в культуральной среде,
где более 4,5% клеток в указанной обогащенной популяции экспрессируют GGT-1, и
где более 80% клеток в указанной обогащенной популяции экспрессируют цитокератин.
2. Популяция клеток по п. 1, где VEGF, KIM-1, GGT-1 и цитокератин являются детектируемыми с помощью моноклонального или поликлонального антитела.
3. Популяция клеток по п. 1, где более 18% клеток экспрессируют GGT-1.
4. Популяция клеток по п. 1, где цитокератин выбирают из CK8, CK18, CK19 и их комбинаций.
5. Популяция клеток по п. 4, где более 80% экспрессируют CK18.
6. Популяция клеток по п. 1, где цитокератин является детектируемым с помощью поликлонального антитела.
7. Популяция клеток по п. 1, дополнительно отличающаяся экспрессией AQP клетками в указанной обогащенной популяции.
8. Популяция клеток по п. 1, дополнительно отличающаяся тем, что менее 40% клеток в указанной обогащенной популяции экспрессируют AQP2.
9. Популяция клеток по п. 7, где AQP2 является детектируемым с помощью моноклонального антитела.
10. Популяция клеток по п. 1, где указанный образец почки млекопитающего представляет собой образец почки человека.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261718150P | 2012-10-24 | 2012-10-24 | |
| US61/718,150 | 2012-10-24 | ||
| US201361876616P | 2013-09-11 | 2013-09-11 | |
| US61/876,616 | 2013-09-11 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015119514A Division RU2702721C2 (ru) | 2012-10-24 | 2013-10-24 | Популяции клеток почек и их применение |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2023125277A Division RU2023125277A (ru) | 2012-10-24 | 2023-10-03 | Популяции клеток почек и их применение |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2019118791A true RU2019118791A (ru) | 2019-07-11 |
| RU2805243C2 RU2805243C2 (ru) | 2023-10-12 |
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