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RU2018144231A - A method of constructing biocatalyst strains having nitrilase activity and increased operational stability, a recombinant bacterial strain Rhodococcus rhodochrous obtained in this way, a method for the synthesis of acrylic acid using this strain as a biocatalyst - Google Patents

A method of constructing biocatalyst strains having nitrilase activity and increased operational stability, a recombinant bacterial strain Rhodococcus rhodochrous obtained in this way, a method for the synthesis of acrylic acid using this strain as a biocatalyst Download PDF

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Publication number
RU2018144231A
RU2018144231A RU2018144231A RU2018144231A RU2018144231A RU 2018144231 A RU2018144231 A RU 2018144231A RU 2018144231 A RU2018144231 A RU 2018144231A RU 2018144231 A RU2018144231 A RU 2018144231A RU 2018144231 A RU2018144231 A RU 2018144231A
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Russia
Prior art keywords
biocatalyst
strain
constructing
synthesis
acrylic acid
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RU2018144231A
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Russian (ru)
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RU2731289C2 (en
RU2018144231A3 (en
Inventor
Александр Степанович Яненко
Сергей Петрович Воронин
Андрей Дмитриевич Новиков
Сергей Алексеевич Глинский
Константин Валерьевич Лавров
Рубен Арменович Минасян
Original Assignee
Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика)
Акционерное Общество "Биоамид" (АО "Биоамид")
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Application filed by Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика), Акционерное Общество "Биоамид" (АО "Биоамид") filed Critical Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика)
Priority to RU2018144231A priority Critical patent/RU2731289C2/en
Publication of RU2018144231A publication Critical patent/RU2018144231A/en
Publication of RU2018144231A3 publication Critical patent/RU2018144231A3/ru
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Publication of RU2731289C2 publication Critical patent/RU2731289C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (3)

1. Способ конструирования штамма-биокатализатора, обладающего нитрилазной активностью и повышенной операционной стабильностью, путем интеграции в геном бактерий Rhodococcus rhodochrous двух копий гена нитрилазы из штамма Alcaligenes denitrificans С-32 VKM B-2243D.1. A method for constructing a biocatalyst strain having nitrilase activity and increased operational stability by integrating two copies of the nitrilase gene from the Alcaligenes denitrificans C-32 VKM B-2243D strain into the Rhodococcus rhodochrous bacterial genome. 2. Рекомбинантный штамм бактерий Rhodococcus rhodochrous ВКПМ АС-2111, полученный способом по п. 1.2. Recombinant strain of bacteria Rhodococcus rhodochrous VKPM AC-2111, obtained by the method according to p. 1. 3. Способ синтеза акриловой кислоты в форме акрилата аммония из акрилонитрила с использованием в качестве биокатализатора рекомбинантного штамма бактерий по п. 2.3. The method of synthesis of acrylic acid in the form of ammonium acrylate from acrylonitrile using as a biocatalyst a recombinant strain of bacteria according to claim 2.
RU2018144231A 2018-12-13 2018-12-13 Method for constructing a biocatalyst strain based on bacteria of the genus rhodococcus, having nitrilase activity and high operational stability, recombinant strain of rhodococcus rhodochrous bacteria produced by such method, a method for synthesis of acrylic acid using this strain as a biocatalyst RU2731289C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2018144231A RU2731289C2 (en) 2018-12-13 2018-12-13 Method for constructing a biocatalyst strain based on bacteria of the genus rhodococcus, having nitrilase activity and high operational stability, recombinant strain of rhodococcus rhodochrous bacteria produced by such method, a method for synthesis of acrylic acid using this strain as a biocatalyst

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2018144231A RU2731289C2 (en) 2018-12-13 2018-12-13 Method for constructing a biocatalyst strain based on bacteria of the genus rhodococcus, having nitrilase activity and high operational stability, recombinant strain of rhodococcus rhodochrous bacteria produced by such method, a method for synthesis of acrylic acid using this strain as a biocatalyst

Publications (3)

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RU2018144231A true RU2018144231A (en) 2020-06-15
RU2018144231A3 RU2018144231A3 (en) 2020-06-15
RU2731289C2 RU2731289C2 (en) 2020-09-01

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RU2018144231A RU2731289C2 (en) 2018-12-13 2018-12-13 Method for constructing a biocatalyst strain based on bacteria of the genus rhodococcus, having nitrilase activity and high operational stability, recombinant strain of rhodococcus rhodochrous bacteria produced by such method, a method for synthesis of acrylic acid using this strain as a biocatalyst

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Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004295409B2 (en) * 2003-12-02 2010-07-29 Ciba Specialty Chemicals Water Treatments Limited Strain of Rhodococcus rhodochrous NCIMB 41164 and its use as producer of nitrile hydratase
RU2323977C2 (en) * 2006-01-30 2008-05-10 Закрытое акционерное общество "Ашленд МСП" Biotechnical method of ammonium acrylate generating
RU2337954C1 (en) * 2007-04-19 2008-11-10 Закрытое акционерное общество "Биоамид" Strain of bacteria alcaligenes denitrificans -nitrilase producent

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RU2731289C2 (en) 2020-09-01
RU2018144231A3 (en) 2020-06-15

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