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RU2017105590A - METHOD FOR PCR-RFLP ANALYSIS FOR GENOTYPING OF CATTLE BY RPS3A GENE - Google Patents

METHOD FOR PCR-RFLP ANALYSIS FOR GENOTYPING OF CATTLE BY RPS3A GENE Download PDF

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Publication number
RU2017105590A
RU2017105590A RU2017105590A RU2017105590A RU2017105590A RU 2017105590 A RU2017105590 A RU 2017105590A RU 2017105590 A RU2017105590 A RU 2017105590A RU 2017105590 A RU2017105590 A RU 2017105590A RU 2017105590 A RU2017105590 A RU 2017105590A
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Russia
Prior art keywords
pcr
rps3a
genotyping
cattle
rflp analysis
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RU2017105590A
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Russian (ru)
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RU2667124C2 (en
RU2017105590A3 (en
Inventor
Егор Яковлевич Лебедько
Ольга Александровна Епишко
Витольд Казимирович Пестис
Людмила Александровна Танана
Екатерина Сергеевна Чебуранова
Наталья Александровна Сонич
Мурат Юрьевич Шевченко
Original Assignee
Федеральное государственное бюджетное образовательное учреждение высшего образования "Брянский государственный аграрный университет"
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Priority to RU2017105590A priority Critical patent/RU2667124C2/en
Publication of RU2017105590A publication Critical patent/RU2017105590A/en
Publication of RU2017105590A3 publication Critical patent/RU2017105590A3/ru
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Publication of RU2667124C2 publication Critical patent/RU2667124C2/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Claims (7)

Способ проведения ПЦР-ПДРФ-анализа для генотипирования крупного рогатого скота по гену RPS3A, отличающийся тем, что на этапе ПЦР используются другие последовательности олигонуклеотидов:The method of PCR-RFLP analysis for genotyping of cattle by the RPS3A gene, characterized in that at the PCR stage, other oligonucleotide sequences are used: RPS3A 1:5' - GGC CAG GGA CCG TGT ТСА TTG AGG АСА ТС - 3'RPS3A 1: 5 '- GGC CAG GGA CCG TGT TCA TTG AGG ASA TC - 3' RPS3A 2: 5' - ТТС CTG GGA ССС CGT GAG АСА CAT ACT TG - 3',RPS3A 2: 5 '- TTC CTG GGA CCC CGT GAG ACA CAT ACT TG - 3', Используется иная ПЦР-программа:A different PCR program is used: х1: 94°С - 5 минx1: 94 ° C - 5 min х30: 94°С - 30 сек, 55°С - 30 сек, 72°С - 30 секx30: 94 ° C - 30 sec, 55 ° C - 30 sec, 72 ° C - 30 sec х1: 72°С - 10 мин.x1: 72 ° C - 10 min.
RU2017105590A 2017-02-20 2017-02-20 Method of conducting of pcr-rflp analysis for genotyping of large cattle by rps3a gene RU2667124C2 (en)

Priority Applications (1)

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RU2017105590A RU2667124C2 (en) 2017-02-20 2017-02-20 Method of conducting of pcr-rflp analysis for genotyping of large cattle by rps3a gene

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Application Number Priority Date Filing Date Title
RU2017105590A RU2667124C2 (en) 2017-02-20 2017-02-20 Method of conducting of pcr-rflp analysis for genotyping of large cattle by rps3a gene

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RU2017105590A true RU2017105590A (en) 2018-08-20
RU2017105590A3 RU2017105590A3 (en) 2018-08-20
RU2667124C2 RU2667124C2 (en) 2018-09-14

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Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2429275C (en) * 2000-11-16 2006-04-11 Christian Bendixen Genetic test for the identification of carriers of complex vertebral malformations in cattle
RU2528743C1 (en) * 2013-01-29 2014-09-20 Рамиль Ришадович Вафин Method of carrying out pcr-rflp for genotyping cattle on alleles a and k of gene dgat1

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Publication number Publication date
RU2667124C2 (en) 2018-09-14
RU2017105590A3 (en) 2018-08-20

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Effective date: 20200221