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RU2015154352A - GENETIC DESIGN FOR EXPRESSION OF GENES IN CELLS OF THE INSECT POLYPEDILUM VANDERPLANKI - Google Patents

GENETIC DESIGN FOR EXPRESSION OF GENES IN CELLS OF THE INSECT POLYPEDILUM VANDERPLANKI Download PDF

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Publication number
RU2015154352A
RU2015154352A RU2015154352A RU2015154352A RU2015154352A RU 2015154352 A RU2015154352 A RU 2015154352A RU 2015154352 A RU2015154352 A RU 2015154352A RU 2015154352 A RU2015154352 A RU 2015154352A RU 2015154352 A RU2015154352 A RU 2015154352A
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RU
Russia
Prior art keywords
insect
cells
expression
genes
genetic design
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RU2015154352A
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Russian (ru)
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RU2626590C2 (en
Inventor
Такахиро Кикавада
Олег Александрович Гусев
Ришар Корнетт
Елена Ильясовна Шагимарданова
Окада Джун
Согаме Йоичиро
Александр Александрович Несмелов
Original Assignee
федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ)
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Priority to RU2015154352A priority Critical patent/RU2626590C2/en
Publication of RU2015154352A publication Critical patent/RU2015154352A/en
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Publication of RU2626590C2 publication Critical patent/RU2626590C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (3)

1. Генетическая конструкция для экспрессии генов в клетках насекомого Polypedilum vanderplanki, включающая выделенный из последовательности ДНК Polypedilum vanderplanki промотор Р121.1. A genetic construct for gene expression in Polypedilum vanderplanki insect cells, comprising the P121 promoter isolated from the Polypedilum vanderplanki DNA sequence. 2. Генетическая конструкция по п. 1, отличающаяся тем, что промотор Р121 заменяют на аналогичный промотор со сходством нуклеотидной последовательности не менее 86%.2. The genetic construct according to claim 1, characterized in that the P121 promoter is replaced with a similar promoter with a similar nucleotide sequence of at least 86%. 3. Генетическая конструкция по п. 2, отличающаяся тем, что в качестве аналогичного промотора используют промотор P-II.3. The genetic construct according to claim 2, characterized in that the P-II promoter is used as a similar promoter.
RU2015154352A 2015-12-18 2015-12-18 Genetic design for expression of genes in cells of insects polypedilum vanderplanki RU2626590C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2015154352A RU2626590C2 (en) 2015-12-18 2015-12-18 Genetic design for expression of genes in cells of insects polypedilum vanderplanki

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2015154352A RU2626590C2 (en) 2015-12-18 2015-12-18 Genetic design for expression of genes in cells of insects polypedilum vanderplanki

Publications (2)

Publication Number Publication Date
RU2015154352A true RU2015154352A (en) 2017-06-22
RU2626590C2 RU2626590C2 (en) 2017-07-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2015154352A RU2626590C2 (en) 2015-12-18 2015-12-18 Genetic design for expression of genes in cells of insects polypedilum vanderplanki

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2763049C1 (en) * 2021-03-22 2021-12-27 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Genetic structure for increasing radiation resistance of insect cell culture p. vanderplanki
RU2766887C1 (en) * 2021-03-29 2022-03-16 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Genetic construct with apoptosis inhibitor protein intended for expression of foreign genes in cells of insect polypedilum vanderplanki in conditions of high radiation background

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69829189T2 (en) * 1997-03-27 2006-02-16 The University Of British Columbia, Vancouver INSECT EXPRESSION VECTORS
US7473769B2 (en) * 2004-09-08 2009-01-06 National Institute Of Agrobiological Sciences Insect desiccation resistance genes and uses thereof
TWI477602B (en) * 2006-02-09 2015-03-21 Educational Foundation Jichi Medical Univ Novel viral vector
CN102203264A (en) * 2008-09-02 2011-09-28 替代基因表达有限公司(艾尔基尼克斯) Insect-derived promoters for foreign proteins expression in insect cells

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RU2626590C2 (en) 2017-07-28

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