RU2013114374A - Дифференцирование плюрипотентных стволовых клеток - Google Patents
Дифференцирование плюрипотентных стволовых клеток Download PDFInfo
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- RU2013114374A RU2013114374A RU2013114374/10A RU2013114374A RU2013114374A RU 2013114374 A RU2013114374 A RU 2013114374A RU 2013114374/10 A RU2013114374/10 A RU 2013114374/10A RU 2013114374 A RU2013114374 A RU 2013114374A RU 2013114374 A RU2013114374 A RU 2013114374A
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- 230000004069 differentiation Effects 0.000 title claims abstract 7
- 210000001778 pluripotent stem cell Anatomy 0.000 title claims abstract 7
- 210000004027 cell Anatomy 0.000 claims abstract 12
- 239000003112 inhibitor Substances 0.000 claims abstract 8
- 230000000968 intestinal effect Effects 0.000 claims abstract 5
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims abstract 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract 4
- 210000001900 endoderm Anatomy 0.000 claims abstract 4
- 229930002330 retinoic acid Natural products 0.000 claims abstract 4
- 229960001727 tretinoin Drugs 0.000 claims abstract 4
- 210000003890 endocrine cell Anatomy 0.000 claims abstract 2
- 210000000130 stem cell Anatomy 0.000 claims abstract 2
- SNFYYXUGUBUECJ-UHFFFAOYSA-N N-{4-[2-ethyl-1-(1,2,4-triazol-1-yl)butyl]phenyl}-1,3-benzothiazol-2-amine Chemical group C=1C=C(NC=2SC3=CC=CC=C3N=2)C=CC=1C(C(CC)CC)N1C=NC=N1 SNFYYXUGUBUECJ-UHFFFAOYSA-N 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 210000000496 pancreas Anatomy 0.000 claims 1
- 210000002438 upper gastrointestinal tract Anatomy 0.000 abstract 2
- 101100001670 Emericella variicolor andE gene Proteins 0.000 abstract 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 230000009996 pancreatic endocrine effect Effects 0.000 abstract 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract 1
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- C12N5/06—Animal cells or tissues; Human cells or tissues
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Abstract
1. Способ получения популяции клеток-предшественников эндокринных клеток поджелудочной железы из плюрипотентных стволовых клеток, состоящий из стадий:a. Культивирования популяции плюрипотентных стволовых клеток;b. Дифференцирования популяции плюрипотентных стволовых клеток в популяцию клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы;с. Дифференцирования популяции клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы, в популяцию клеток первичной кишечной трубки;D. Дифференцирования популяции клеток первичной кишечной трубки в популяцию клеток заднего сегмента передней кишки; иE. Культивирования популяции клеток задней части передней кишки в среде с добавлением агента, расщепляющего ретиноевую кислоту.2. Способ по п. 1, в котором агент, расщепляющий ретиноевую кислоту, представляет собой ингибитор CYP26A.3. Способ по п. 2, в котором ингибитор CYP26A используют в концентрации от приблизительно 1 нмоль/л до приблизительно 1000 нмоль/л.4. Способ по п. 2, в котором ингибитор CYP26A используют в концентрации от приблизительно 10 нмоль/л до приблизительно 100 нмоль/л.5. Способ по п. 2, в котором ингибитор CYP26A представляет собой N-{4-[2-этил-1-(1H-1, 2, 4-триазол-1-ил)бутил]фенил}-1, 3-бензотиазол-2-амин.
Claims (5)
1. Способ получения популяции клеток-предшественников эндокринных клеток поджелудочной железы из плюрипотентных стволовых клеток, состоящий из стадий:
a. Культивирования популяции плюрипотентных стволовых клеток;
b. Дифференцирования популяции плюрипотентных стволовых клеток в популяцию клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы;
с. Дифференцирования популяции клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы, в популяцию клеток первичной кишечной трубки;
D. Дифференцирования популяции клеток первичной кишечной трубки в популяцию клеток заднего сегмента передней кишки; и
E. Культивирования популяции клеток задней части передней кишки в среде с добавлением агента, расщепляющего ретиноевую кислоту.
2. Способ по п. 1, в котором агент, расщепляющий ретиноевую кислоту, представляет собой ингибитор CYP26A.
3. Способ по п. 2, в котором ингибитор CYP26A используют в концентрации от приблизительно 1 нмоль/л до приблизительно 1000 нмоль/л.
4. Способ по п. 2, в котором ингибитор CYP26A используют в концентрации от приблизительно 10 нмоль/л до приблизительно 100 нмоль/л.
5. Способ по п. 2, в котором ингибитор CYP26A представляет собой N-{4-[2-этил-1-(1H-1, 2, 4-триазол-1-ил)бутил]фенил}-1, 3-бензотиазол-2-амин.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37848010P | 2010-08-31 | 2010-08-31 | |
| US61/378,480 | 2010-08-31 | ||
| PCT/US2011/048131 WO2012030540A2 (en) | 2010-08-31 | 2011-08-17 | Differentiation of pluripotent stem cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016135361A Division RU2673946C1 (ru) | 2010-08-31 | 2011-08-17 | Дифференцирование плюрипотентных стволовых клеток |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2013114374A true RU2013114374A (ru) | 2014-10-10 |
| RU2599420C2 RU2599420C2 (ru) | 2016-10-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013114374/10A RU2599420C2 (ru) | 2010-08-31 | 2011-08-17 | Дифференцирование плюрипотентных стволовых клеток |
| RU2016135361A RU2673946C1 (ru) | 2010-08-31 | 2011-08-17 | Дифференцирование плюрипотентных стволовых клеток |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016135361A RU2673946C1 (ru) | 2010-08-31 | 2011-08-17 | Дифференцирование плюрипотентных стволовых клеток |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US9181528B2 (ru) |
| EP (1) | EP2611907B1 (ru) |
| JP (2) | JP6133776B2 (ru) |
| KR (1) | KR101836855B1 (ru) |
| CN (1) | CN103154237B (ru) |
| AR (1) | AR082821A1 (ru) |
| AU (1) | AU2011296383B2 (ru) |
| BR (1) | BR112013004614A2 (ru) |
| CA (1) | CA2809305C (ru) |
| ES (1) | ES2585028T3 (ru) |
| MX (1) | MX348537B (ru) |
| PH (1) | PH12013500349A1 (ru) |
| PL (1) | PL2611907T3 (ru) |
| RU (2) | RU2599420C2 (ru) |
| SG (1) | SG187947A1 (ru) |
| WO (1) | WO2012030540A2 (ru) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| CA3114827C (en) | 2007-07-31 | 2023-09-05 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic endocrine |
| CA2954431C (en) | 2007-11-27 | 2021-08-24 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic cells |
| RU2551772C2 (ru) | 2008-02-21 | 2015-05-27 | Сентокор Орто Байотек Инк. | Способы, поверхностно-модифицированные носители и композиции для иммобилизации, культивирования и открепления клеток |
| AU2009267137A1 (en) | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
| MX2011004565A (es) | 2008-10-31 | 2011-07-28 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| MX2011004563A (es) | 2008-10-31 | 2011-06-01 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| BRPI0921996A2 (pt) | 2008-11-20 | 2015-08-18 | Centocor Ortho Biotech Inc | Métodos e composições para cultura e ligação de células em substratos planos. |
| EP3260534A1 (en) | 2008-11-20 | 2017-12-27 | Janssen Biotech, Inc. | Pluripotent stem cell culture on micro-carriers |
| EP2456862A4 (en) | 2009-07-20 | 2013-02-27 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS |
| BR112012017761A2 (pt) | 2009-12-23 | 2015-09-15 | Centocor Ortho Biotech Inc | diferenciação das células-tronco embrionárias humanas |
| US9969981B2 (en) | 2010-03-01 | 2018-05-15 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| RU2663339C1 (ru) | 2010-05-12 | 2018-08-03 | Янссен Байотек, Инк. | Дифференцирование эмбриональных стволовых клеток человека |
| KR101836850B1 (ko) | 2010-08-31 | 2018-03-09 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| CA2809300A1 (en) | 2010-08-31 | 2012-03-08 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| AU2012355698B2 (en) | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| RU2018128383A (ru) | 2012-03-07 | 2019-03-14 | Янссен Байотек, Инк. | Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток |
| AR090970A1 (es) * | 2012-05-07 | 2014-12-17 | Janssen Biotech Inc | Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico |
| RU2018108850A (ru) | 2012-06-08 | 2019-02-26 | Янссен Байотек, Инк. | Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки |
| KR102058259B1 (ko) * | 2012-06-26 | 2019-12-23 | 세라시스, 인코포레이티드 | 인슐린 의존성 당뇨병의 치료에 유용한 줄기 세포 및 췌장 세포 |
| JP6470687B2 (ja) * | 2012-09-03 | 2019-02-13 | ノヴォ ノルディスク アー／エス | 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製 |
| EP4039798A1 (en) | 2012-12-31 | 2022-08-10 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells |
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| EP3149156B1 (en) | 2014-05-28 | 2021-02-17 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| EP3204489A4 (en) * | 2014-10-08 | 2018-09-05 | Agency for Science, Technology and Research | Methods of differentiating stem cells into liver cell lineages |
| JP6804438B2 (ja) | 2014-10-17 | 2020-12-23 | チルドレンズ ホスピタル メディカル センター | 多能性幹細胞を使用するヒト小腸のｉｎ ｖｉｖｏモデル、並びにそれを作製、及び使用する方法 |
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| PL2611907T3 (pl) | 2016-11-30 |
| KR101836855B1 (ko) | 2018-04-19 |
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| SG187947A1 (en) | 2013-03-28 |
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| CN103154237A (zh) | 2013-06-12 |
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| EP2611907A4 (en) | 2014-01-22 |
| EP2611907B1 (en) | 2016-05-04 |
| EP2611907A2 (en) | 2013-07-10 |
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| JP6353951B2 (ja) | 2018-07-04 |
| AU2011296383A1 (en) | 2013-03-07 |
| PH12013500349A1 (en) | 2022-03-30 |
| CN103154237B (zh) | 2016-03-16 |
| BR112013004614A2 (pt) | 2024-01-16 |
| JP6133776B2 (ja) | 2017-05-24 |
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| US9181528B2 (en) | 2015-11-10 |
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| CA2809305C (en) | 2019-06-11 |
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| CA2809305A1 (en) | 2012-03-08 |
| JP2013536687A (ja) | 2013-09-26 |
| MX2013002407A (es) | 2013-04-05 |
| US20120052576A1 (en) | 2012-03-01 |
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