RU2012117014A - METHOD FOR PRODUCING RECOMBINANT CAPSIDE PROTEIN OF HEPATITIS VIRUS E. RECOMBINANT VACCINE FOR PREVENTION OF VIRAL HEPATITIS E. - Google Patents
METHOD FOR PRODUCING RECOMBINANT CAPSIDE PROTEIN OF HEPATITIS VIRUS E. RECOMBINANT VACCINE FOR PREVENTION OF VIRAL HEPATITIS E. Download PDFInfo
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- RU2012117014A RU2012117014A RU2012117014/10A RU2012117014A RU2012117014A RU 2012117014 A RU2012117014 A RU 2012117014A RU 2012117014/10 A RU2012117014/10 A RU 2012117014/10A RU 2012117014 A RU2012117014 A RU 2012117014A RU 2012117014 A RU2012117014 A RU 2012117014A
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- Prior art keywords
- recombinant
- protein
- hepatitis
- orf2
- rthev
- Prior art date
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract 15
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 9
- 108010008038 Synthetic Vaccines Proteins 0.000 title claims abstract 5
- 229940124551 recombinant vaccine Drugs 0.000 title claims abstract 5
- 206010019799 Hepatitis viral Diseases 0.000 title claims abstract 3
- 230000002265 prevention Effects 0.000 title claims abstract 3
- 201000001862 viral hepatitis Diseases 0.000 title claims abstract 3
- 241000700605 Viruses Species 0.000 title 1
- 208000006454 hepatitis Diseases 0.000 title 1
- 231100000283 hepatitis Toxicity 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 241000724675 Hepatitis E virus Species 0.000 claims abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 4
- 241000320412 Ogataea angusta Species 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims abstract 4
- 239000000178 monomer Substances 0.000 claims abstract 4
- 108090000565 Capsid Proteins Proteins 0.000 claims abstract 2
- 101710167800 Capsid assembly scaffolding protein Proteins 0.000 claims abstract 2
- 102100023321 Ceruloplasmin Human genes 0.000 claims abstract 2
- 101900111963 Hepatitis E virus Capsid protein Proteins 0.000 claims abstract 2
- 101710113540 ORF2 protein Proteins 0.000 claims abstract 2
- 101710090523 Putative movement protein Proteins 0.000 claims abstract 2
- 239000002671 adjuvant Substances 0.000 claims abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 239000000427 antigen Substances 0.000 claims abstract 2
- 102000036639 antigens Human genes 0.000 claims abstract 2
- 108091007433 antigens Proteins 0.000 claims abstract 2
- 239000003085 diluting agent Substances 0.000 claims abstract 2
- 238000002955 isolation Methods 0.000 claims abstract 2
- 239000003550 marker Substances 0.000 claims abstract 2
- 229920001184 polypeptide Polymers 0.000 claims abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 2
- 238000000746 purification Methods 0.000 claims abstract 2
- 150000007944 thiolates Chemical class 0.000 claims abstract 2
- 229960005486 vaccine Drugs 0.000 claims abstract 2
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Способ получения рекомбинантного капсидного белка вируса гепатита Е генотипа 3 (rtHEV-ORF2), включающий культивирование штамма-продуцента, содержащего последовательность ДНК, кодирующую белок ORF2, в подходящих для экспрессии указанного белка условиях, выделение и очистку целевого белка, отличающийся тем, что культивируют рекомбинантный штамм дрожжей Hansenula polymorpha КБТ-11/pHEV-001, содержащий интегрированную в геном последовательность ДНК, включающую рекомбинантный ген, кодирующий полипептид rtHEV-ORF2 с аминокислотной последовательностью SEQ ID NO:1, под контролем промотора гена МОХ Hansenula polymorpha и ген LEU2 S.cerevisiae в качестве селективного маркера, а после выделения и очистки получают белок rtHEV-ORF2 в виде VLP, состоящей из мономеров с молекулярной массой мономера 56 кДа.2. Рекомбинантная вакцина для профилактики вирусного гепатита Е, содержащая эффективное количество капсидного белка вируса гепатита Е, адъювант и физиологически приемлемый разбавитель, отличающаяся тем, что в качестве антигена вируса гепатита Е вакцина содержит белок rtHEV-ORF2, полученный по способу п.1.3. Рекомбинантная вакцина по п.2, отличающаяся тем, что дополнительно содержит мертиолят.1. A method of producing a recombinant hepatitis E virus capsid protein of genotype 3 (rtHEV-ORF2), comprising cultivating a producer strain containing a DNA sequence encoding an ORF2 protein under conditions suitable for expression of said protein, isolating and purifying the target protein, characterized in that cultivate a recombinant strain of Hansenula polymorpha KBT-11 / pHEV-001 containing the DNA sequence integrated into the genome, including the recombinant gene encoding the rtHEV-ORF2 polypeptide with the amino acid sequence of SEQ ID NO: 1, under warning light gene promoter and MOX Hansenula polymorpha LEU2 S.cerevisiae gene as a selectable marker, and after isolation and purification rtHEV-ORF2 protein in a VLP, consisting of monomers with a molecular mass of 56 kDa.2 monomer. Recombinant vaccine for the prevention of viral hepatitis E, containing an effective amount of the capsid protein of the hepatitis E virus, an adjuvant and a physiologically acceptable diluent, characterized in that the vaccine contains the rtHEV-ORF2 protein obtained by the method of clause 1.3 as an antigen of the hepatitis E virus. The recombinant vaccine according to claim 2, characterized in that it further comprises thiolate.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2012117014/10A RU2501809C1 (en) | 2012-04-27 | 2012-04-27 | Method for obtaining recombinant core protein of hepatitis e virus and recombinant vaccine for prophylaxis of hepatitis e virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2012117014/10A RU2501809C1 (en) | 2012-04-27 | 2012-04-27 | Method for obtaining recombinant core protein of hepatitis e virus and recombinant vaccine for prophylaxis of hepatitis e virus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2012117014A true RU2012117014A (en) | 2013-11-10 |
| RU2501809C1 RU2501809C1 (en) | 2013-12-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012117014/10A RU2501809C1 (en) | 2012-04-27 | 2012-04-27 | Method for obtaining recombinant core protein of hepatitis e virus and recombinant vaccine for prophylaxis of hepatitis e virus |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2501809C1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023231149A1 (en) * | 2022-05-25 | 2023-12-07 | Xuzhou Medical University | Recombinant orthohepevirus genotype c1 orf2 protein and preparation method therefor and use thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2745558C2 (en) * | 2016-12-27 | 2021-03-29 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр исследований и разработки иммунобиологических препаратов им. М.П. Чумакова РАН" | Method of producing recombinant capsid protein of hepatitis e virus |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6291641B1 (en) * | 1988-06-17 | 2001-09-18 | Genelabs Technologies, Inc. | Hepatitis E virus antigens and uses therefor |
| US6455492B1 (en) * | 1988-06-17 | 2002-09-24 | Genelabs Technologies, Inc. | Hepatitis E virus vaccine and method |
| US6458562B1 (en) * | 1998-04-09 | 2002-10-01 | The United States Of America As Represented By The Secretary Of Health And Human Services | Recombinant proteins of a Pakistani strain of hepatitis E and their use in diagnostic methods and vaccines |
| RU2252748C1 (en) * | 2004-02-11 | 2005-05-27 | Солонский Александр Владимирович | Method for treating viral hepatitis |
| CN101062941A (en) * | 2007-04-27 | 2007-10-31 | 东南大学 | Recombined hepatitis E hepatitis virus protein, preparation method and usage thereof |
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2012
- 2012-04-27 RU RU2012117014/10A patent/RU2501809C1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023231149A1 (en) * | 2022-05-25 | 2023-12-07 | Xuzhou Medical University | Recombinant orthohepevirus genotype c1 orf2 protein and preparation method therefor and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2501809C1 (en) | 2013-12-20 |
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