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RU2010138610A - STABILIZATION OF DEHYDROGENASES BY STABLE COFERENCES - Google Patents

STABILIZATION OF DEHYDROGENASES BY STABLE COFERENCES Download PDF

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RU2010138610A
RU2010138610A RU2010138610/10A RU2010138610A RU2010138610A RU 2010138610 A RU2010138610 A RU 2010138610A RU 2010138610/10 A RU2010138610/10 A RU 2010138610/10A RU 2010138610 A RU2010138610 A RU 2010138610A RU 2010138610 A RU2010138610 A RU 2010138610A
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dehydrogenase
enzyme
independently
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stable coenzyme
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Дитер ХАЙНДЛЬ (DE)
Дитер ХАЙНДЛЬ
Карина ХОРН (DE)
Карина ХОРН
Клаудиа ГЕССЛЕР-ДИЧЕ (DE)
Клаудиа ГЕССЛЕР-ДИЧЕ
Йоахим ХЕНЕС (DE)
Йоахим ХЕНЕС
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Ф.Хоффманн-Ля Рош Аг (Ch)
Ф.Хоффманн-Ля Рош Аг
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Abstract

1. Способ стабилизации фермента, отличающийся тем, что фермент хранят в присутствии стабильного кофермента. ! 2. Способ по п.1, отличающийся тем, что фермент выбран из дегидрогеназ. ! 3. Способ по п.2, отличающийся тем, что фермент представляет собой дегидрогеназу, выбранную из глюкозодегидрогеназы (Е.С.1.1.1.47), лактатдегидрогеназы (Е.С.1.1.1.27, 1.1.1.28), малатдегидрогеназы (Е.С.1.1.1.37), глицериндегидрогеназы (Е.С.1.1.1.6), алкогольдегидрогеназы (Е.С.1.1.1.1), альфа-гидроксибутиратдегидрогеназы, сорбитдегидрогеназы или дегидрогеназы аминокислоты, например дегидрогеназы L-аминокислоты (Е.С.1.4.1.5). ! 4. Способ по любому из пп.1-3, отличающийся тем, что в качестве фермента используют глюкозодегидрогеназу. ! 5. Способ по любому из пп.1-4, отличающийся тем, что стабильный кофермент выбран из стабильных соединений никотинамидадениндинуклеотида (NAD/NADH), и соединений никотинамидадениндинуклеотидфосфата (NADP/NADPH), и соединения формулы (I) ! ! 6. Способ по п.5, отличающийся тем, что стабильный кофермент выбран из соединений, имеющих общую формулу (II) ! ! в которой ! A=аденин или его аналог, ! T=в каждом случае независимо O, S, ! U=в каждом случае независимо OH, SH, BH3 -, BCNH2 -, ! V=в каждом случае независимо OH или фосфатная группа или две группы, образующие циклическую фосфатную группу; ! W=COOR, CON(R)2, COR, CSN(R)2, где R=в каждом случае независимо Н или С1-С2-алкил, ! Х2, Х2=в каждом случае независимо О, СН2, СНСН3, С(СН3)2, NH, NCH3, ! Y=NH, S, O, CH2, ! Z=линейный или циклический органический радикал, при условии, что Z и пиридиновый остаток не связаны посредством гликозидной связи, или его соль, или, где целесообразно, его восстановленная форма. ! 7. Способ по п.6, в котором Z выбран из ! (i) линейного радик 1. The method of stabilization of the enzyme, characterized in that the enzyme is stored in the presence of a stable coenzyme. ! 2. The method according to claim 1, characterized in that the enzyme is selected from dehydrogenases. ! 3. The method according to claim 2, characterized in that the enzyme is a dehydrogenase selected from glucose dehydrogenase (E.C.1.1.1.47), lactate dehydrogenase (E.C.1.1.1.27, 1.1.1.28), malate dehydrogenase (E.C. .1.1.1.37), glycerin dehydrogenase (E.C.1.1.1.6), alcohol dehydrogenase (E.C.1.1.1.1), alpha-hydroxybutyrate dehydrogenase, sorbitol dehydrogenase or amino acid dehydrogenase, e.g. L-amino acid dehydrogenase (E.C.1.4.1.5 ) ! 4. The method according to any one of claims 1 to 3, characterized in that glucose dehydrogenase is used as the enzyme. ! 5. The method according to any one of claims 1 to 4, characterized in that the stable coenzyme is selected from stable compounds of nicotinamide adenine dinucleotide (NAD / NADH), and compounds of nicotinamide adenine dinucleotide phosphate (NADP / NADPH), and a compound of formula (I)! ! 6. The method according to claim 5, characterized in that the stable coenzyme is selected from compounds having the general formula (II)! ! wherein ! A = adenine or its analog,! T = in each case, independently O, S,! U = in each case independently OH, SH, BH3 -, BCNH2 -,! V = in each case, independently OH or a phosphate group or two groups forming a cyclic phosphate group; ! W = COOR, CON (R) 2, COR, CSN (R) 2, where R = in each case independently H or C1-C2-alkyl,! X2, X2 = in each case, independently O, CH2, CHCH3, C (CH3) 2, NH, NCH3,! Y = NH, S, O, CH2,! Z = a linear or cyclic organic radical, provided that Z and the pyridine residue are not linked via a glycosidic bond, or a salt thereof, or, where appropriate, its reduced form. ! 7. The method according to claim 6, in which Z is selected from! (i) linear radic

Claims (24)

1. Способ стабилизации фермента, отличающийся тем, что фермент хранят в присутствии стабильного кофермента.1. The method of stabilization of the enzyme, characterized in that the enzyme is stored in the presence of a stable coenzyme. 2. Способ по п.1, отличающийся тем, что фермент выбран из дегидрогеназ.2. The method according to claim 1, characterized in that the enzyme is selected from dehydrogenases. 3. Способ по п.2, отличающийся тем, что фермент представляет собой дегидрогеназу, выбранную из глюкозодегидрогеназы (Е.С.1.1.1.47), лактатдегидрогеназы (Е.С.1.1.1.27, 1.1.1.28), малатдегидрогеназы (Е.С.1.1.1.37), глицериндегидрогеназы (Е.С.1.1.1.6), алкогольдегидрогеназы (Е.С.1.1.1.1), альфа-гидроксибутиратдегидрогеназы, сорбитдегидрогеназы или дегидрогеназы аминокислоты, например дегидрогеназы L-аминокислоты (Е.С.1.4.1.5).3. The method according to claim 2, characterized in that the enzyme is a dehydrogenase selected from glucose dehydrogenase (E.C.1.1.1.47), lactate dehydrogenase (E.C.1.1.1.27, 1.1.1.28), malate dehydrogenase (E.C. .1.1.1.37), glycerin dehydrogenase (E.C.1.1.1.6), alcohol dehydrogenase (E.C.1.1.1.1), alpha-hydroxybutyrate dehydrogenase, sorbitol dehydrogenase or amino acid dehydrogenase, e.g. L-amino acid dehydrogenase (E.C.1.4.1.5 ) 4. Способ по любому из пп.1-3, отличающийся тем, что в качестве фермента используют глюкозодегидрогеназу.4. The method according to any one of claims 1 to 3, characterized in that glucose dehydrogenase is used as the enzyme. 5. Способ по любому из пп.1-4, отличающийся тем, что стабильный кофермент выбран из стабильных соединений никотинамидадениндинуклеотида (NAD/NADH), и соединений никотинамидадениндинуклеотидфосфата (NADP/NADPH), и соединения формулы (I)5. The method according to any one of claims 1 to 4, characterized in that the stable coenzyme is selected from stable compounds of nicotinamide adenine dinucleotide (NAD / NADH), and compounds of nicotinamide adenine dinucleotide phosphate (NADP / NADPH), and a compound of formula (I)
Figure 00000001
Figure 00000001
 6. Способ по п.5, отличающийся тем, что стабильный кофермент выбран из соединений, имеющих общую формулу (II)6. The method according to claim 5, characterized in that the stable coenzyme is selected from compounds having the general formula (II)
Figure 00000002
Figure 00000002
 в которойwherein A=аденин или его аналог,A = adenine or its analogue T=в каждом случае независимо O, S,T = in each case, independently O, S, U=в каждом случае независимо OH, SH, BH3 -, BCNH2 -,U = in each case independently OH, SH, BH 3 - , BCNH 2 - , V=в каждом случае независимо OH или фосфатная группа или две группы, образующие циклическую фосфатную группу;V = in each case, independently OH or a phosphate group or two groups forming a cyclic phosphate group; W=COOR, CON(R)2, COR, CSN(R)2, где R=в каждом случае независимо Н или С1-С2-алкил,W = COOR, CON (R) 2 , COR, CSN (R) 2 , where R = in each case independently H or C 1 -C 2 alkyl, Х2, Х2=в каждом случае независимо О, СН2, СНСН3, С(СН3)2, NH, NCH3,X 2 , X 2 = in each case, independently O, CH 2 , CHCH 3 , C (CH 3 ) 2 , NH, NCH 3 , Y=NH, S, O, CH2,Y = NH, S, O, CH 2 , Z=линейный или циклический органический радикал, при условии, что Z и пиридиновый остаток не связаны посредством гликозидной связи, или его соль, или, где целесообразно, его восстановленная форма.Z = a linear or cyclic organic radical, provided that Z and the pyridine residue are not linked via a glycosidic bond, or a salt thereof, or, where appropriate, its reduced form. 7. Способ по п.6, в котором Z выбран из7. The method according to claim 6, in which Z is selected from (i) линейного радикала, имеющего 4-6 атомов C, в которых 1 или 2 атома C необязательно замещены одним или несколькими гетероатомами, выбранными из O, S и N, и(i) a linear radical having 4-6 C atoms in which 1 or 2 C atoms are optionally substituted with one or more heteroatoms selected from O, S and N, and (ii) радикала, включающего циклическую группу, которая имеет 5 или 6 атомов C, которая необязательно содержит гетероатом, выбранный из O, S и N, и необязательно один или несколько заместителей, и радикала CR4 2, где CR4 2 связан с циклической группой и с Х2, где R4=в каждом случае независимо H, F, Cl, CH3.(ii) a radical comprising a cyclic group that has 5 or 6 C atoms, which optionally contains a heteroatom selected from O, S and N, and optionally one or more substituents, and a CR 4 2 radical, where CR 4 2 is attached to a cyclic a group and with X 2 , where R 4 = in each case independently H, F, Cl, CH 3 . 8. Способ по п.7, в котором Z представляет собой соединение общей формулы (III)8. The method according to claim 7, in which Z is a compound of General formula (III)
Figure 00000003
Figure 00000003
 в которой одинарная или двойная связь может присутствовать между R5' и R5”, гдеin which a single or double bond may be present between R 5 'and R 5 ” , where R4=в каждом случае независимо H, F, Cl, CH3,R 4 = in each case independently H, F, Cl, CH 3 , R5=CR4 2,R 5 = CR 4 2 , где R5'=O, S, NH, NC1-C2-алкил, CR4 2, СНОН, СНОСН3, иwhere R 5 ′ = O, S, NH, NC 1 -C 2 -alkyl, CR 4 2 , CHOH, CHOC 3 , and R5”=CR4 2, СНОН, СНОСН3, если существует одинарная связь между R5' и R5”,R 5 ”= CR 4 2 , CHOH, CHOC 3 , if there is a single bond between R 5 'and R 5” , где R5'=R5”=CR4, если существует двойная связь между R5' и R5”, иwhere R 5 '= R 5 ” = CR 4 if there is a double bond between R 5 ' and R 5” , and R6, R6'=в каждом случае независимо СН или ССН3.R 6 , R 6 '= in each case, independently CH or CCH 3 . 9. Способ по п.6, в котором W=CONH2 или COCH3.9. The method according to claim 6, in which W = CONH 2 or COCH 3 . 10. Способ по п.8 или 9, в котором R5 представляет собой СН2.10. The method of claim 8 or 9, in which R 5 represents CH 2 . 11. Способ по п.8, в котором R5' выбран из CH2, CHOH и NH.11. The method of claim 8, in which R 5 'is selected from CH 2 , CHOH and NH. 12. Способ по п.8, в котором R5' и R5” каждый представляет собой СНОН.12. The method of claim 8, in which R 5 'and R 5 ” each represents CHON. 13. Способ по п.8, в котором R5' представляет собой NH и R5” представляет собой СН2.13. The method of claim 8, in which R 5 'represents NH and R 5 ” represents CH 2 . 14. Способ по п.1, отличающийся тем, что стабильный кофермент представляет собой карбаNAD.14. The method according to claim 1, characterized in that the stable coenzyme is NAD carb. 15. Способ по п.1, отличающийся тем, что фермент, стабилизированный стабильным коферментом, хранят в течение периода по меньшей мере 2 недель.15. The method according to claim 1, characterized in that the enzyme stabilized with a stable coenzyme is stored for a period of at least 2 weeks. 16. Способ по п.1, отличающийся тем, что фермент, стабилизированный стабильным коферментом, хранят при температуре по меньшей мере 20°C.16. The method according to claim 1, characterized in that the enzyme stabilized with a stable coenzyme is stored at a temperature of at least 20 ° C. 17. Способ по п.1, отличающийся тем, что фермент, стабилизированный стабильным коферментом, хранят без осушающего реагента.17. The method according to claim 1, characterized in that the enzyme stabilized with a stable coenzyme is stored without a drying reagent. 18. Способ по п.1, отличающийся тем, что фермент, стабилизированный стабильным коферментом, хранят при относительной влажности, составляющей по меньшей мере 50%.18. The method according to claim 1, characterized in that the enzyme stabilized with a stable coenzyme is stored at a relative humidity of at least 50%. 19. Способ по п.1, отличающийся тем, что хранение фермента, стабилизированного стабильным коферментом, происходит в виде сухого вещества.19. The method according to claim 1, characterized in that the storage of the enzyme stabilized with a stable coenzyme occurs as a dry substance. 20. Способ по п.1, отличающийся тем, что хранение фермента, стабилизированного стабильным коферментом, происходит в жидкой фазе.20. The method according to claim 1, characterized in that the storage of the enzyme stabilized with a stable coenzyme occurs in the liquid phase. 21. Способ по п.1, отличающийся тем, что хранение фермента, стабилизированного стабильным коферментом, происходит в тестовом элементе.21. The method according to claim 1, characterized in that the storage of the enzyme stabilized with a stable coenzyme occurs in the test element. 22. Фермент, который стабилизирован стабильным коферментом, отличающийся тем, что показывает при хранении в течение по меньшей мере 2 недель, при температуре по меньшей мере 20°C, где необходимо, при высокой влажности и без осушающего реагента, снижение ферментативной активности, составляющее менее чем 50%, по сравнению с начальным уровнем, где фермент представляет собой подвергнутую мутации глюкозодегидрогеназу, и подвергнутая мутации глюкозодегидрогеназа имеет увеличенную термическую или гидролитическую стабильность по сравнению с соответствующей глюкозодегидрогеназой дикого типа.22. An enzyme that is stabilized with a stable coenzyme, characterized in that it shows a decrease in enzymatic activity of less than 2 months when stored for at least 2 weeks, at a temperature of at least 20 ° C, where necessary, at high humidity and without a drying agent, than 50%, compared with the initial level, where the enzyme is a mutated glucose dehydrogenase, and the mutated glucose dehydrogenase has increased thermal or hydrolytic stability compared to the corresponding etstvuyuschey wildtype glucose dehydrogenase. 23. Детекторный реагент для обнаружения анализируемого вещества, который содержит стабилизированный фермент по п.22.23. A detector reagent for detecting an analyte that contains a stabilized enzyme according to claim 22. 24. Тестовый элемент, отличающийся тем, что содержит стабилизированный фермент по п.22 или детекторный реагент по п.23. 24. A test element, characterized in that it contains the stabilized enzyme according to item 22 or the detection reagent according to item 23.
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