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RU2010152769A - METHOD FOR DETERMINING EFFECTIVENESS OF BIODEGRADATION OF OIL HYDROCARBONS IN NATIVE AND POLLUTED SOILS - Google Patents

METHOD FOR DETERMINING EFFECTIVENESS OF BIODEGRADATION OF OIL HYDROCARBONS IN NATIVE AND POLLUTED SOILS Download PDF

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Publication number
RU2010152769A
RU2010152769A RU2010152769/15A RU2010152769A RU2010152769A RU 2010152769 A RU2010152769 A RU 2010152769A RU 2010152769/15 A RU2010152769/15 A RU 2010152769/15A RU 2010152769 A RU2010152769 A RU 2010152769A RU 2010152769 A RU2010152769 A RU 2010152769A
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Russia
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soil
oil
carbon dioxide
amount
hydrocarbons
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RU2010152769/15A
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Russian (ru)
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RU2477472C2 (en
Inventor
Анатолий Маркович Зякун (RU)
Анатолий Маркович Зякун
Владимир Васильевич Кочетков (RU)
Владимир Васильевич Кочетков
Александр Михайлович Боронин (RU)
Александр Михайлович Боронин
Original Assignee
Учреждение Российской академии наук Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (RU)
Учреждение Российской академии наук Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук
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Priority to RU2010152769/15A priority Critical patent/RU2477472C2/en
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Abstract

Способ определения эффективности биодеградации углеводородов нефти в почвах, заключающийся в презентативном отборе образцов нативной или загрязненной поллютантами почвы, отличающийся тем, что для оценки углеводород-окисляющей активности почвенной микробиоты: 1) образец тестируемой почвы помещают в закрытую емкость, в течение определенного времени фиксируют количество метаболической углекислоты до и после внесения тестового субстрата (углеводородов нефти), 2) измеряют количество потребленного кислорода в газовой фазе, 3) измеряют характеристики изотопного состава углерода метаболической углекислоты до и после внесения тестового субстрата, 4) проводят материально-изотопный баланс для определения количества углерода углекислоты, включенного за счет минерализации почвенного органического вещества и углеводородов нефти, 5) используя скорость микробной деградации нефти, проводят длительный прогноз микробного потребления нефти почвенной микробиоты на период положительных годовых температур анализируемого региона. A method for determining the effectiveness of biodegradation of oil hydrocarbons in soils, which consists in presentational sampling of soil native or polluted with pollutants, characterized in that for assessing the hydrocarbon-oxidizing activity of soil microbiota: 1) a sample of the tested soil is placed in a closed container, the amount of metabolic carbon dioxide before and after making the test substrate (oil hydrocarbons), 2) measure the amount of oxygen consumed in the gas phase, 3) measure the char the characteristics of the carbon isotopic composition of the metabolic carbon dioxide before and after applying the test substrate, 4) carry out a material-isotopic balance to determine the amount of carbon dioxide included in the mineralization of soil organic matter and hydrocarbons, 5) using the rate of microbial degradation of oil, conduct a long-term forecast of microbial consumption oil of soil microbiota for a period of positive annual temperatures of the analyzed region.

Claims (1)

Способ определения эффективности биодеградации углеводородов нефти в почвах, заключающийся в презентативном отборе образцов нативной или загрязненной поллютантами почвы, отличающийся тем, что для оценки углеводород-окисляющей активности почвенной микробиоты: 1) образец тестируемой почвы помещают в закрытую емкость, в течение определенного времени фиксируют количество метаболической углекислоты до и после внесения тестового субстрата (углеводородов нефти), 2) измеряют количество потребленного кислорода в газовой фазе, 3) измеряют характеристики изотопного состава углерода метаболической углекислоты до и после внесения тестового субстрата, 4) проводят материально-изотопный баланс для определения количества углерода углекислоты, включенного за счет минерализации почвенного органического вещества и углеводородов нефти, 5) используя скорость микробной деградации нефти, проводят длительный прогноз микробного потребления нефти почвенной микробиоты на период положительных годовых температур анализируемого региона. A method for determining the effectiveness of biodegradation of oil hydrocarbons in soils, which consists in presentational sampling of soil native or polluted with pollutants, characterized in that for assessing the hydrocarbon-oxidizing activity of soil microbiota: 1) a sample of the tested soil is placed in a closed container, the amount of metabolic carbon dioxide before and after making the test substrate (oil hydrocarbons), 2) measure the amount of oxygen consumed in the gas phase, 3) measure the char the characteristics of the carbon isotopic composition of the metabolic carbon dioxide before and after applying the test substrate, 4) carry out a material-isotopic balance to determine the amount of carbon dioxide included in the mineralization of soil organic matter and hydrocarbons, 5) using the rate of microbial degradation of oil, conduct a long-term forecast of microbial consumption oil of soil microbiota for a period of positive annual temperatures of the analyzed region.
RU2010152769/15A 2010-12-24 2010-12-24 Method to detect efficiency of biodegradation of oil hydrocarbons in native and contaminated soils RU2477472C2 (en)

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RU2010152769/15A RU2477472C2 (en) 2010-12-24 2010-12-24 Method to detect efficiency of biodegradation of oil hydrocarbons in native and contaminated soils

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CN113138172A (en) * 2020-01-20 2021-07-20 天津理工大学 Method for testing saline-alkali soil remediation rate
CN113533683A (en) * 2021-06-02 2021-10-22 广东新泓环境咨询有限公司 Surface soil petroleum hydrocarbon pollution early warning method and system
CN115575596A (en) * 2022-09-27 2023-01-06 上海城市水资源开发利用国家工程中心有限公司 A system and method for estimating the amount of degradation of insoluble oils
CN116603843A (en) * 2023-05-26 2023-08-18 北京建工环境修复股份有限公司 Monitoring method of in-situ site repair process

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CN104298868B (en) * 2014-09-30 2018-05-18 北京农业信息技术研究中心 The frozen meat shelf life Forecasting Methodology and system of a kind of Cold Chain Logistics
RU2590554C1 (en) * 2014-12-19 2016-07-10 Федеральное государственное бюджетное учреждение науки Институт механики Уральского отделения Российской академии наук Method of analysing characteristics of the behaviour of contaminants in soil
RU2599131C1 (en) * 2015-04-08 2016-10-10 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт агрохимии им. Д.Н. Прянишникова" (ФГБНУ "ВНИИ агрохимии") Method of making standard samples of weight ratio of oil products in quartz sand
RU2641825C2 (en) * 2016-04-05 2018-01-22 Федеральное государственное бюджетное учреждение науки Институт механики Уральского отделения Российской академии наук Method of testing polluted soils
CN114814184A (en) * 2022-05-10 2022-07-29 中国科学院城市环境研究所 A method for determining the degradation rate of degradable plastics based on carbon 13 isotope method

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RU2199406C2 (en) * 2001-04-25 2003-02-27 Институт биологии Уфимского научного центра РАН Method of cleaning grounds from oil pollution
DE102004014621A1 (en) * 2004-03-25 2005-10-13 H.C. Starck Gmbh Organic compounds with core-shell structure
RU2279472C2 (en) * 2004-06-25 2006-07-10 Открытое акционерное общество "Акционерная нефтяная компания "Башнефть" Method of cleaning crude oil-polluted soil
RU2297290C1 (en) * 2005-10-04 2007-04-20 Закрытое акционерное общество научно-производственное предприятие "Биомедхим" (ЗАО НПП "Биомедхим") Method of recultivation of the bleaching soil polluted with the oil products
RU2007110460A (en) * 2007-03-21 2008-09-27 Государственное образовательное учреждение высшего профессионального образовани Иркутский государственный университет путей сообщени (ИрГУПС) (RU) METHOD FOR DETERMINING SOIL POLLUTION BY OIL PRODUCTS THROUGH PHYSICAL AND CHEMICAL SIMULATION
RU2387996C1 (en) * 2008-09-22 2010-04-27 Общество с ограниченной ответственностью "Научно-исследовательский институт природных газов и газовых технологий - Газпром ВНИИГАЗ" (ООО "ВНИИГАЗ") Method of monitoring cleaning of soil contaminated with hydrocarbons and neutralisation of hydrocarbon sludge through analysis of dehydrogenase activity
CN101858902B (en) * 2010-06-01 2012-12-12 中国科学院广州地球化学研究所 Soil alteration carbonate measuring method integrating carbon isotope ratio

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113138172A (en) * 2020-01-20 2021-07-20 天津理工大学 Method for testing saline-alkali soil remediation rate
CN113138172B (en) * 2020-01-20 2022-06-07 天津理工大学 Method for testing saline-alkali soil remediation rate
CN113533683A (en) * 2021-06-02 2021-10-22 广东新泓环境咨询有限公司 Surface soil petroleum hydrocarbon pollution early warning method and system
CN115575596A (en) * 2022-09-27 2023-01-06 上海城市水资源开发利用国家工程中心有限公司 A system and method for estimating the amount of degradation of insoluble oils
CN116603843A (en) * 2023-05-26 2023-08-18 北京建工环境修复股份有限公司 Monitoring method of in-situ site repair process

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