RU2009118872A - STRAIN AND METHOD FOR OBTAINING MITOMYCIN ANTIBIOTIC WITH BIOSYNTHESIS - Google Patents
STRAIN AND METHOD FOR OBTAINING MITOMYCIN ANTIBIOTIC WITH BIOSYNTHESIS Download PDFInfo
- Publication number
- RU2009118872A RU2009118872A RU2009118872/10A RU2009118872A RU2009118872A RU 2009118872 A RU2009118872 A RU 2009118872A RU 2009118872/10 A RU2009118872/10 A RU 2009118872/10A RU 2009118872 A RU2009118872 A RU 2009118872A RU 2009118872 A RU2009118872 A RU 2009118872A
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- Prior art keywords
- mitomycin
- strain
- medium
- seed
- fermentation medium
- Prior art date
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- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 title claims abstract 29
- 229960004857 mitomycin Drugs 0.000 title claims abstract 15
- 238000000034 method Methods 0.000 title claims abstract 9
- 229930192392 Mitomycin Natural products 0.000 title 1
- 230000003115 biocidal effect Effects 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract 6
- 238000000855 fermentation Methods 0.000 claims abstract 6
- 230000004151 fermentation Effects 0.000 claims abstract 6
- 239000000203 mixture Substances 0.000 claims abstract 6
- 235000015097 nutrients Nutrition 0.000 claims abstract 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract 4
- 229920002472 Starch Polymers 0.000 claims abstract 4
- 241000521327 Streptomyces caespitosus Species 0.000 claims abstract 4
- 241000209149 Zea Species 0.000 claims abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 4
- 238000005273 aeration Methods 0.000 claims abstract 4
- 235000005822 corn Nutrition 0.000 claims abstract 4
- 235000013312 flour Nutrition 0.000 claims abstract 4
- 239000008107 starch Substances 0.000 claims abstract 4
- 235000019698 starch Nutrition 0.000 claims abstract 4
- 230000001954 sterilising effect Effects 0.000 claims abstract 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract 4
- 239000008399 tap water Substances 0.000 claims abstract 3
- 235000020679 tap water Nutrition 0.000 claims abstract 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 2
- 229930006000 Sucrose Natural products 0.000 claims abstract 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract 2
- 239000003242 anti bacterial agent Substances 0.000 claims abstract 2
- 229940088710 antibiotic agent Drugs 0.000 claims abstract 2
- 238000005119 centrifugation Methods 0.000 claims abstract 2
- 235000019441 ethanol Nutrition 0.000 claims abstract 2
- 238000001914 filtration Methods 0.000 claims abstract 2
- 239000008103 glucose Substances 0.000 claims abstract 2
- 239000002054 inoculum Substances 0.000 claims abstract 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Natural products OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract 2
- 244000005700 microbiome Species 0.000 claims abstract 2
- 239000011780 sodium chloride Substances 0.000 claims abstract 2
- 239000005720 sucrose Substances 0.000 claims abstract 2
- 239000006228 supernatant Substances 0.000 claims abstract 2
- 239000007836 KH2PO4 Substances 0.000 abstract 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 2
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 abstract 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 abstract 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 abstract 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Штамм - продуцент митомицина С Streptomyces caespitosus 3810/0Е, депонированный в Коллекции культур микроорганизмов Государственного учреждения Научно-исследовательский Институт по изысканию новых антибиотиков им. Г.Ф.Гаузе Российской Академии медицинских наук (ГУ НИИНА РАМН) под номером ИНА 01082. ! 2. Способ получения митомицина С, в котором используется штамм - продуцент митомицина С по п.1 и питательные среды, разработанные для этого штамма: посевная среда следующего состава, %: глюкоза - 0,6-1,4, крахмал - 0,6-1,4, соевая мука - 2-5, кукурузный экстракт - 0,6-1,0, KH2PO4 - 0,01-0,03, мел - 0,05-0,12, (NH4)2SO4 - 0,06-0,14, вода водопроводная, рН до стерилизации 6,8-7,2, и ферментационная среда следующего состава, %: крахмал - 0,6-1,4, сахароза - 1-3, соевая мука - 2-4, кукурузный экстракт - 0,6-1,0, CoCl2·6H2O - 0,007-0012, KH2PO4 - 0,02, мел - 0,05-0,12, (NH4)2SO4 - 0,06-0,14, FeSO4·7H2O - 0,01-0,02, MgSO4·7H2O - 0,01-0,03, NaCl - 0,2-0,6, вода водопроводная, рН до стерилизации 6,8-7,2. ! 3. Способ получения митомицина С, в котором использован штамм по п.1 и питательные среды по п.2, отличающийся тем, что посевной материал выращивают 40-52 ч на посевной среде в условиях аэрирования и передают в количестве 2-7 об.% в ферментационную среду, на которой выращивают в условиях аэрирования в течение 100-120 ч, после чего мицелий удаляют центрифугированием или фильтрацией, а надосадочную жидкость экстрагируют смесью ацетонитрила и метилового или этилового спирта. ! 4. Способ получения митомицина С, отличающийся тем, что при использовании штамма по п.1, питательных посевной и ферментационных сред по п.2, способа культивирования штамма Streptomyces caespitosus ИНА и выделения митомицина С по п.3 получают от 85 до 100 мкг/мл митомицина С. 1. The strain is the producer of mitomycin C Streptomyces caespitosus 3810 / 0Е, deposited in the Collection of microorganism cultures of the State institution Research Institute for the search for new antibiotics named after GF Gauze of the Russian Academy of Medical Sciences (GU NIINA RAMS) under the number INA 01082.! 2. The method of obtaining mitomycin C, which uses the strain - producer of mitomycin C according to claim 1 and the nutrient medium developed for this strain: seed medium of the following composition,%: glucose - 0.6-1.4, starch - 0.6 -1.4, soy flour - 2-5, corn extract - 0.6-1.0, KH2PO4 - 0.01-0.03, chalk - 0.05-0.12, (NH4) 2SO4 - 0, 06-0.14, tap water, pH before sterilization of 6.8-7.2, and a fermentation medium of the following composition,%: starch - 0.6-1.4, sucrose - 1-3, soy flour - 2-4 corn extract - 0.6-1.0, CoCl2.6H2O - 0.007-0012, KH2PO4 - 0.02, chalk - 0.05-0.12, (NH4) 2SO4 - 0.06-0.14, FeSO4 7H2O - 0.01-0.02, MgSO4.7H2O - 0.01-0.03, NaCl - 0.2-0.6, water, water supply pH, before sterilization, 6.8-7.2. ! 3. The method of producing mitomycin C, in which the strain according to claim 1 and the nutrient medium according to claim 2, wherein the seed is grown for 40-52 hours on the seed medium under aeration conditions and is transferred in an amount of 2-7 vol.% in a fermentation medium, which is grown under aeration conditions for 100-120 hours, after which the mycelium is removed by centrifugation or filtration, and the supernatant is extracted with a mixture of acetonitrile and methyl or ethyl alcohol. ! 4. A method of producing mitomycin C, characterized in that when using the strain according to claim 1, the nutrient inoculum and fermentation medium according to claim 2, a method of cultivating a strain of Streptomyces caespitosus INA and isolating mitomycin C according to claim 3 receive from 85 to 100 μg / ml mitomycin C.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009118872/10A RU2420568C2 (en) | 2009-05-20 | 2009-05-20 | Strain and biosynthesis method of producing antibiotic mitomycin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009118872/10A RU2420568C2 (en) | 2009-05-20 | 2009-05-20 | Strain and biosynthesis method of producing antibiotic mitomycin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2009118872A true RU2009118872A (en) | 2010-11-27 |
| RU2420568C2 RU2420568C2 (en) | 2011-06-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009118872/10A RU2420568C2 (en) | 2009-05-20 | 2009-05-20 | Strain and biosynthesis method of producing antibiotic mitomycin |
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| Country | Link |
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| RU (1) | RU2420568C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317361A (en) * | 2021-12-31 | 2022-04-12 | 北方民族大学 | Streptomyces new strain and separation method and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2614126C1 (en) * | 2016-03-24 | 2017-03-22 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт по изысканию новых антибиотиков имени Г.Ф. Гаузе" | Fungi strain from sordariomycetes class - producer of eremoxylarin a antibiotic |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2050384B (en) * | 1979-04-02 | 1983-04-07 | Bristol Myers Co | Antitumor antibacterial agents |
| SU1272993A3 (en) * | 1984-04-05 | 1986-11-23 | Юниверсити Патентс Инк (Фирма) | Method of producing mitomycin derivatives |
| US6495348B1 (en) * | 1993-10-07 | 2002-12-17 | Regents Of The University Of Minnesota | Mitomycin biosynthetic gene cluster |
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2009
- 2009-05-20 RU RU2009118872/10A patent/RU2420568C2/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317361A (en) * | 2021-12-31 | 2022-04-12 | 北方民族大学 | Streptomyces new strain and separation method and application thereof |
| CN114317361B (en) * | 2021-12-31 | 2023-09-22 | 北方民族大学 | A new strain of Streptomyces and its isolation method and application |
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| Publication number | Publication date |
|---|---|
| RU2420568C2 (en) | 2011-06-10 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20120521 |