RU2004248C1 - Method for preparation of biologically-active substances possessing stimulating and adaptogenic effect - Google Patents

Description

2. The anti-inflammatory effect of the extract of Alma-Ata Hawthorn was studied in accordance with the methodological recommendations for the experimental (preclinical) study of non-steroidal anti-inflammatory pharmacological substances.

As an analogue, the well-known non-steroidal antiflogistic - glycyram was used. Clinical efficacy is assessed by their complex effect on the inflammatory process. An integrated assessment of the activity of the tested extract is presented in the form of sums of the relative values of each of the studied parameters in comparison with the analogue in Table 3. Moreover, aspirin activity was taken as 1. It was shown that the extract of Alma-Ata Hawthorn exceeded the glycerol analogue in terms of the severity of anti-inflammatory activity. The analgesic activity of the drug was also noted.

3. The stimulating effect of the extract of Alma-Ata Hawthorn was carried out by recording the swimming time of animals until they were completely fatigued (until death).

The stimulating effect in experiments on mice was established at a dose of 5 ml / kg. The duration of swimming of experimental animals significantly exceeded the control by 66%. With a single intragastric administration of the extract to rats, the greatest effect was achieved at a dose of 2.5 ml / kg, the swimming duration increased by 70% compared with control (01).

After repeated administration over 5 days at a dose of 5 ml / kg, the swimming time of animals exceeded the control by 78% (, 05).

With increasing doses in experiments on mice and rats, the activity of the extract decreased.

Comparative studies of the stimulating effect of the extract of Hawthorn Alma-Ata with the analogue extract of Eleutherococcus have shown that the advantage of the test preparation is not 19-36%.

4. Adzptogenic effect is established on mice of the Csr line. subjected to subcooling in a special chamber. Compared with eleutherococcus, the adaptogenic effect of the use of extract of hawthorn is higher. When studying the increase in body resistance to a physical factor - cooling, the following data were obtained: animals that received the studied extract at a dose of 10 ml / kg lived 35% longer compared to the control and 17% longer compared to eleutherococcus.

The technical result of using this method is that; that a new herbal remedy was obtained which has low toxicity (practically non-toxic) with a complex of anti-inflammatory, stimulating and adaptogenic effects, which can be widely used in medicine as a therapeutic and prophylactic agent.

(56) Hammerman A.F. and other wild medicinal plants. M .: Medicine, 1976.p.166-171.

Tsinovskis R.E., Baltic Sea Towers. Riga. 1971.

Krylov G.V. The herbs of life and their seekers. Novosibirsk 1969.

Fruit to leaf ratio

1: 1 1: 2 1: 3 1: 4

Table 1

The output of extractive substances. %

14:16

26:27

29:30

2 & 2E.5

Fruit to leaf ratio

1:50

1:75

1: 100

1: 125

Comparison of the anti-inflammatory properties of the ecgtraject of the Hawthorn Epma Atinga glycyrams and aspirin

Note: We have had a 50% reduction in inflammation.

Claims (1)

The claims METHOD FOR PRODUCING BIOLOGICALLY ACTIVE SUBSTANCES POSSESSING STIMULATING ADAPTOGENIC ACTION by extraction of plant materials, characterized in that the extraction is carried out table 2 The yield of extractives,% 25:26 28:29 30:31 29.5: 29 T a b p i c e 3 fruits and leaves of the Alma-Ata Hawthorn (Grategus almaatmicus) with a ratio of 1: 3 and a raw material-extractant ratio of 1 100, the process is carried out at 95 - 90 ° C, followed by purification, thickening and drying of the extract to a powder state.