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RU2004129290A - Разрушение гена redk - Google Patents

Разрушение гена redk Download PDF

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Publication number
RU2004129290A
RU2004129290A RU2004129290/13A RU2004129290A RU2004129290A RU 2004129290 A RU2004129290 A RU 2004129290A RU 2004129290/13 A RU2004129290/13 A RU 2004129290/13A RU 2004129290 A RU2004129290 A RU 2004129290A RU 2004129290 A RU2004129290 A RU 2004129290A
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RU
Russia
Prior art keywords
cell
animal cell
animal
redk
gene
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RU2004129290/13A
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English (en)
Inventor
Уилль м Говард БРИССЕТТ (US)
Уилльям Говард БРИССЕТТ
Кулдип Сингх НЕОТЕ (US)
Кулдип Сингх НЕОТЕ
Джон Эдвард ХЭМБОР (US)
Джон Эдвард ХЭМБОР
Мелани Рут АЛЛЕН (US)
Мелани Рут АЛЛЕН
Марша Линн РОУЧ (US)
Марша Линн РОУЧ
Original Assignee
Пфайзер Продактс Инк. (Us)
Пфайзер Продактс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Пфайзер Продактс Инк. (Us), Пфайзер Продактс Инк. filed Critical Пфайзер Продактс Инк. (Us)
Publication of RU2004129290A publication Critical patent/RU2004129290A/ru

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
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    • C12N2517/02—Cells from transgenic animals
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
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    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (10)

1. Генетически модифицированное, не относящееся к человеку млекопитающее, где модификация включает в себя разрушение гена REDK.
2. Млекопитающее по п.1, где указанное млекопитающее представляет собой мышь.
3. Генетически модифицированная животная клетка, в которой модификация включает в себя разрушение гена REDK.
4. Животная клетка по п.3, где указанная клетка представляет собой ES-клетку или ES-подобную клетку.
5. Животная клетка по п.3, где указанная клетка выделена из генетически модифицированного, не относящегося к человеку млекопитающего по п.1.
6. Животная клетка по п.3, где указанная клетка представляет собой кроветворную клетку или клетку семенников.
7. Животная клетка по п.3, где указанная клетка является мышиной или человеческой [клеткой].
8. Способ идентификации биологической характеристики, ассоциированной со снижением или отменой активности REDK, подразумевающий сравнение биологической характеристики генетически модифицированного, не относящегося к человеку млекопитающего по п.1 или генетически модифицированной животной клетки по п.3, с характеристикой подходящего контроля дикого типа, представляющего собой не относящееся к человеку животное или животную клетку.
9. Способ идентификации гена, который демонстрирует модифицированную экспрессию в результате снижения или отмены активности REDK, подразумевающий сравнение уровня экспрессии по меньшей мере одного гена, отличного от REDK, в животной клетке по п.3, с экспрессией данного гена в подходящей животной клетке дикого типа служащей контролем.
10. Способ идентификации белка, который демонстрирует модифицированный уровень или посттрансляционный процессинг в результате снижения активности REDK в животной клетке, подразумевающий сравнение уровня или посттрансляционных характеристик данного белка в животной клетке по п.3 с уровнем или посттрансляционными характеристиками данного белка в подходящей животной клетке дикого типа, служащей контролем.
RU2004129290/13A 2002-03-30 2003-03-17 Разрушение гена redk RU2004129290A (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36881002P 2002-03-30 2002-03-30
US60/368,810 2002-03-30

Publications (1)

Publication Number Publication Date
RU2004129290A true RU2004129290A (ru) 2005-04-10

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ID=28675543

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RU2004129290/13A RU2004129290A (ru) 2002-03-30 2003-03-17 Разрушение гена redk

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US (1) US20030188325A1 (ru)
EP (1) EP1361270A3 (ru)
JP (1) JP2003310261A (ru)
KR (1) KR20040094879A (ru)
CN (1) CN1646906A (ru)
AU (1) AU2003209590A1 (ru)
CA (1) CA2480286A1 (ru)
HR (1) HRP20040901A2 (ru)
NO (1) NO20044522L (ru)
RU (1) RU2004129290A (ru)
WO (1) WO2003083038A2 (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200115705A1 (en) 2017-06-06 2020-04-16 Zymergen Inc. A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa

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Publication number Publication date
CA2480286A1 (en) 2003-10-09
CN1646906A (zh) 2005-07-27
EP1361270A3 (en) 2004-01-02
EP1361270A2 (en) 2003-11-12
HRP20040901A2 (en) 2004-12-31
KR20040094879A (ko) 2004-11-10
US20030188325A1 (en) 2003-10-02
WO2003083038A3 (en) 2004-02-05
WO2003083038A2 (en) 2003-10-09
JP2003310261A (ja) 2003-11-05
AU2003209590A1 (en) 2003-10-13
NO20044522L (no) 2004-10-21

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