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RU2003102398A - METHOD FOR DETERMINING THE ACID RESISTANCE OF ERYTHROCYTES - Google Patents

METHOD FOR DETERMINING THE ACID RESISTANCE OF ERYTHROCYTES

Info

Publication number
RU2003102398A
RU2003102398A RU2003102398/15A RU2003102398A RU2003102398A RU 2003102398 A RU2003102398 A RU 2003102398A RU 2003102398/15 A RU2003102398/15 A RU 2003102398/15A RU 2003102398 A RU2003102398 A RU 2003102398A RU 2003102398 A RU2003102398 A RU 2003102398A
Authority
RU
Russia
Prior art keywords
blood cells
red blood
acid resistance
determining
hemolysis
Prior art date
Application number
RU2003102398/15A
Other languages
Russian (ru)
Other versions
RU2242760C2 (en
Inventor
Алексей Владимирович Андриенко
Владимир Валерьевич Усынин
Елена Борисовна Науменко
Original Assignee
Алтайский государственный медицинский университет
Filing date
Publication date
Application filed by Алтайский государственный медицинский университет filed Critical Алтайский государственный медицинский университет
Priority to RU2003102398/15A priority Critical patent/RU2242760C2/en
Priority claimed from RU2003102398/15A external-priority patent/RU2242760C2/en
Publication of RU2003102398A publication Critical patent/RU2003102398A/en
Application granted granted Critical
Publication of RU2242760C2 publication Critical patent/RU2242760C2/en

Links

Claims (1)

Способ оценки кислотной стойкости эритроцитов путем определения величины светопропускания стандартизированной взвеси эритроцитов после добавления в нее кислотного гемолитика с построением графика гемолиза, отличающийся тем, что стандартизируют взвесь эритроцитов центрифугированием 15 мин и разведением 0,01 мл эритроцитарного осадка в 4,0 мл физиологического раствора, затем регистрируют время максимального размера эритроцитов и среднюю скорость гемолиза, и рассчитывают индекс кислотной стойкости эритроцитов (ИКС) по формулеA method for assessing the acid resistance of red blood cells by determining the transmittance of a standardized suspension of red blood cells after adding an acid hemolytic to it with the construction of a hemolysis schedule, characterized in that the suspension of red blood cells is standardized by centrifugation for 15 minutes and dilution of 0.01 ml of red blood cells in 4.0 ml of physiological solution, then register the time of the maximum size of red blood cells and the average rate of hemolysis, and calculate the acid resistance index of red blood cells (IKS) according to the formula ИКС=t_d max / gV m,IR = t_d max / gV m, где t_d max - время максимального среднего радиуса эритроцитов;where t_d max is the time of the maximum average radius of red blood cells; gV m - средняя скорость гемолиза.gV m is the average rate of hemolysis.
RU2003102398/15A 2003-01-27 2003-01-27 Method for detecting acid stability of erythrocytes RU2242760C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2003102398/15A RU2242760C2 (en) 2003-01-27 2003-01-27 Method for detecting acid stability of erythrocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003102398/15A RU2242760C2 (en) 2003-01-27 2003-01-27 Method for detecting acid stability of erythrocytes

Publications (2)

Publication Number Publication Date
RU2003102398A true RU2003102398A (en) 2004-08-10
RU2242760C2 RU2242760C2 (en) 2004-12-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2003102398/15A RU2242760C2 (en) 2003-01-27 2003-01-27 Method for detecting acid stability of erythrocytes

Country Status (1)

Country Link
RU (1) RU2242760C2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2098819C1 (en) * 1994-08-19 1997-12-10 Саратовский государственный университет им.Н.Г.Чернышевского Method for diagnosing patient digestive tract pathology

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