RU2001953C1 - Method for vitamin b12 preparation production - Google Patents

Abstract

Usage: microbiological industry, feed vitamin B can be used in agriculture SUMMARY OF THE INVENTION: The cupuric liquid obtained by fermentation of the producing strain Propionibaderium shemranii M 82 is concentrated by condensation to a dry matter content of 10-60 wt.% And spray dried at a temperature of 145-165 ° °; S. 2 tab.

Description

The invention relates to the microbiological industry, and the resulting vitamin B12 can be used in agriculture, in particular in the diets of agricultural animals to increase their productivity and as an anti-anemic drug.

A method is known for producing feed vitamin 612, comprising concentrating the culture fluid to a predetermined solids content and drying.

The feed vitamin B12 obtained in a known manner contains only 20-25% of the total amount of cobalamins of the active form of sulfitocobalamin, the remaining 75-80% of cobalamins are biologically inactive forms. The sulfitoform of this vitamin Bi2, as well as other non-natural forms, such as cyanocobalamin and oxycobalin, when used has a number of contraindications for erythrimia and erythrocytosis, and also causes an increase in benign and malignant neoplasms.

The aim of the invention is to increase the yield and quality of the target product.

This is achieved in that in the method for producing the vitamin Bi2 preparation. providing for the concentration of the culture fluid to a predetermined solids content and drying, concentration of the culture fluid obtained by fermentation of the producing strain Propior is carried out. bacterium shermanll M82, to a solids content of from 10 to 60 wt.%, and drying is carried out at a temperature of 145-165 ° C.

Differences between the proposed method and the known one are the following: use of the strain Propionibacterlum shermanll as a product, thereby increasing the yield of the biologically active form of vitamin B12 5-deoxyadenosyl cobamide (coenzyme B12) due to the direction of biological synthesis;

concentration of the culture fluid is carried out to a solids content of May 10 to 60. % and drying is carried out at a temperature of 145-165 ° C.

The proposed limit of the drying temperature avoids thermal degradation of the target component (coenzyme of vitamin 812), therefore, to increase the yield of the active form of vitamin B12 from the culture fluid.

When concentrating the culture fluid to solids below 10%, the process is not technologically advanced and economically disadvantageous due to the low multiplicity (2) of biomass dehydration. Concentration to a solids content of more than 60% is technically difficult to carry out because of the possibility of equipment.

PRI me R 1. In 10 flasks with a capacity of 700 ml load 400 ml of culture medium of the following composition. % to the volume of the medium:

Corn extract. 6.0 Glucose 2.0

CoCl20.0065

Water Else

The medium is inoculated with 72-hour seed of Vitamin 812 Propionibacterlum shermanll M-82 in an amount of 15% by volume of the medium. Cultivation was carried out under anaerobic conditions at 28-30 ° C with periodic titration of the culture fluid to pH 7.0. starting from 18 hours of growth every 9 hours until the end of fermentation with concentrated ammonia. For 72 hours, the precursor 5,6-dimethylbenzimidazole is added and cultivation is continued for another 48 hours. From 36 to 72 hours, 9 g of glucose is supplied every 9 hours to the flask as a 25% solution according to the following program,% to the volume of the medium:

1 feed 0.24

2 feed 0.36

3 feed 0.48

4 feed 0.60

5 feed 0.72

The result is 5160 ml of culture fluid with a concentration of vitamin Bi2 (in coenzyme form) of 23.8 μg / ml. The removal of vitamin 812 123 mg. The solids content in the culture fluid is 5.6% (289 g).

The resulting culture fluid was dried on an Anhydro-type spray dryer with the following drying parameters:

inlet temperature: 150 ° С; outlet temperature: 80 ° С. the flow rate of the solution for drying 4.2 l / h Received 272 g of vitamin Bia powder with a moisture content of 6.7% and a vitamin B 12 content (in coenzyme form) of 103.5 mg. Mass fraction of vitamin Bi2 (in terms of dry weight) 408 mcg / g. Losses Bi2. mechanical ablation 12%, inactivation 4%. PRI me R 2. Vitamin B12 culture fluid is obtained according to the method described in example 1. 5300 ml of culture fluid with a concentration of vitamin Bia (in coenzyme form) 25 1 μg / ml are obtained. We will eat vitamin B and PZ mg Godrzh of dry substances in the culture fluid 6.0% (318 g). The cuyural fluid is evaporated on a film evaporator in vacuum at a temperature of 70 ° C and a residual pressure of 0.02 MPA to a volume of 1650 ml. The solids content in one stripped off culture fluid is 19.3%. One stripped off culture fluid was dried on anhydrodylate-type hairpin with a centrifugal micron method:

inlet temperature VI - C; outlet temperature 75 C; flow rate for drying 1.0 / h. Received 328 g of vitamin Bi2 powder with a moisture content of 7.8% and the content of the engine B12 (in terms of dry sun, g) of 387 μg / g. Loss of Vitamin Bi2. Mechanical entrainment 5%, Inactivation 7%.

EXAMPLE 3. Vitamin Bi2 culture fluid was obtained as in Example 1. 5020 ml of culture fluid with a vitamin B12 concentration (about the immediate form) of 23.2 μg / ml was obtained. Eating with food-p In 116 mg. The dry content of t L: - D i m. Of ural liquid is 6.2% (31 1 rj to yp, the liquid is separated by a separator of type АСГ Consumption of cultural AIDICity for separation of 5 l / h Received 650 ml of biomass with dry content

18 (. BSM “(sui illi dried on with a multi-spray gun (fluidized casing)) Tempura at the inlet of the M5V Pop cappucci 11G g of vitamin Bi2 f powder contains 3.5% of the contents of the diet and 8i2 city food (about coffee) form) 107 ig Mass fraction of vitamin Bi./ (l p.rrscho tg H; J dry gee) 99 I mcg / g. Ptomin losses

017

With 1% ablation,

i iHai tpltsi 4%.

P | and m R r 4, Lounti counteractural fluid according to example 1. Received G070 ml in I.urel fluid with concentration

5 PMGAMMNE PP (in cofermet form) 20.7 g-sh. CI.GM Vitamin Bi 126mg, Sotsrzha-PMO su / ich cavesy o kuyulyarnoy liquid (..t.) -Kir (297 g Culture fluid iltHTp .. fused using a centrifuge type OS-OM

0 1 luchilm 300 sludge biomass of vitamin Bi2 with a solids content of 56%. The biomass of cyiLH-.vt in a dryer with an inert carrier JCMncpai / pa air / ha at the inlet of 165 ° C. Received 165 g of vitamin Bi2 powder with v.iiineM content of 2.0% and a moisture content of Ov i and vigamia B12 (coenzyme forno) 1 (9 gt. Mpcriach calci thiamine 812 (u by | -.cho1n on dry oec ) G73 mm / g Losses 1 agshia P- | t

0M Manpower's accounted for 3.5%

Ll4jiC4iin; jiun10 /

(rjb) / about lrs se sopdedel ““ tpo of the USSR R jG JJ l, S 1 P 10/42, 1932

Temperature at the inlet to the drying chamber ° С

165 150 140 180 190

Table 1 The effect of temperature on DIIXOP, Bi Bi2

Vitamin Bv yield n ready-made

DOJv1 J11

P2 V 89 70 GS

Decrease, h, jr) than inactivation

g.

Decrease, h, jr) than inactivation

.j, „„ - ™

The effect of the solids content in the condensed biomass on the yield of vitamin Bi2

Claims (1)

The claims METHOD FOR PRODUCING VITAMIN B12 PRODUCT, which involves concentrating the culture fluid to a predetermined solids content and drying, characterized in that, for the purpose of Table 2 To increase the yield and quality of the target product, the culture fluid obtained by fermentation of the producing strain of Propionlbacterlum shermanll M-82 is concentrated to a solids content of 10-60 wt.%, and drying is carried out at 145 - 165 C.