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RU2000129650A - BACTERIOLYTIC COMPLEX, METHOD FOR ITS OBTAINING AND STRAIN FOR IMPLEMENTING THE METHOD - Google Patents

BACTERIOLYTIC COMPLEX, METHOD FOR ITS OBTAINING AND STRAIN FOR IMPLEMENTING THE METHOD

Info

Publication number
RU2000129650A
RU2000129650A RU2000129650/13A RU2000129650A RU2000129650A RU 2000129650 A RU2000129650 A RU 2000129650A RU 2000129650/13 A RU2000129650/13 A RU 2000129650/13A RU 2000129650 A RU2000129650 A RU 2000129650A RU 2000129650 A RU2000129650 A RU 2000129650A
Authority
RU
Russia
Prior art keywords
strain
complex
bacteriolytic
xanthomonas campestris
bacterium
Prior art date
Application number
RU2000129650/13A
Other languages
Russian (ru)
Other versions
RU2193063C2 (en
Inventor
Игорь Степанович Кулаев
Ольга Андреевна Степная
Ирина Матвеевна Цфасман
Таисия Сергеевна Черменская
Василий Константинович Акименко
Лариса Александровна Ледова
Людмила Григорьевна Зубрицкая
Original Assignee
Институт биохимии и физиологии микроорганизмов им. Г.К.Скрябина РАН
Filing date
Publication date
Application filed by Институт биохимии и физиологии микроорганизмов им. Г.К.Скрябина РАН filed Critical Институт биохимии и физиологии микроорганизмов им. Г.К.Скрябина РАН
Priority to RU2000129650/13A priority Critical patent/RU2193063C2/en
Priority claimed from RU2000129650/13A external-priority patent/RU2193063C2/en
Priority to PCT/RU2001/000514 priority patent/WO2002044352A1/en
Priority to KR1020027009664A priority patent/KR20020073189A/en
Priority to US10/181,498 priority patent/US7150985B2/en
Priority to CNB018042473A priority patent/CN1266271C/en
Publication of RU2000129650A publication Critical patent/RU2000129650A/en
Application granted granted Critical
Publication of RU2193063C2 publication Critical patent/RU2193063C2/en
Priority to US11/247,553 priority patent/US20060051336A1/en

Links

Claims (3)

1. Бактериолитический комплекс, продуцируемый бактерией вида Xanthomonas campestris, включающий бактериолитические ферменты мурамидазу, мурамоилаланинамидазу, эндопептадазу, протеазу и полисахарид, отличающийся тем, что он продуцируется штаммом бактерии Xanthomonas campesiris ВКМ В-2249 Д и дополнительно содержит бактериолитический фермент с мол. мас. около 22кДа.1. Bacteriolytic complex produced by a bacterium of the species Xanthomonas campestris, including bacteriolytic enzymes muramidase, muramoylalaninamidase, endopeptadase, protease and polysaccharide, characterized in that it is produced by the strain of the bacterium Xanthomonas campesiris VKM B-2249, which is also used in the list of items in addition to the list of items in addition to the table. wt. about 22kD. 2. Способ получения бактериолитического комплекса, включающий культивирование штамма бактерии вида Xanthomonas campestris на питательной среде, отделение биомассы от культуральной жидкости центрифугированием, осаждение комплекса сульфатом аммония, диализ растворенного осадка и лиофильную сушку целевого продукта, отличающийся тем, что культивируют штамм бактерии Xanthomonas campestris ВКМ В-2249 Д, а в качестве питательной среды используют среду, содержащую глюкозу, пептон, дрожжевой экстракт или дрожжевой автолизат, фосфорнокислые соли натрия и калия, сернокислый магний, хлористый калий, сернокислое железо и воду при следующем содержании компонентов, г/л:
Глюкоза - 2-6
Пептон - 1,5-6
Дрожжевой экстракт - 1-6
Или дрожжевой автолизат с аминным азотом 440-490 мг/л - 40-300 мл
Na2HPO412H2O- - 4-5
КН2PO4 - 1-2
MgSO4•7H2O - 4-6
KCl - 0,4-1,6
FеSO4•7Н2О - 0,05-0,2
Вода - До 1 л
осаждение комплекса сульфатом аммония проводят непосредственно из культуральной жидкости.2. A method of obtaining a bacteriolytic complex, including cultivating a strain of the bacterium of the species Xanthomonas campestris on a nutrient medium, separating the biomass from the culture fluid by centrifuging, precipitating the complex with ammonium sulfate, dialysis the dissolved precipitate and freeze drying the target product, characterized in that the strain of Xanthomonas campestris is cultured. -2249 D, and as a nutrient medium using a medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, chamois acid magnesium, potassium chloride, ferrous sulphate and water in the following component ratio, g / l:
Glucose - 2-6
Peptone - 1.5-6
Yeast Extract - 1-6
Or yeast autolysate with amine nitrogen 440-490 mg / l - 40-300 ml
Na 2 HPO 4 12H 2 O- - 4-5
KN 2 PO 4 - 1-2
MgSO 4 • 7H 2 O - 4-6
KCl - 0.4-1.6
FeSO 4 • 7H 2 O - 0.05-0.2
Water - Up to 1 l
precipitation of the complex with ammonium sulfate is carried out directly from the culture fluid. 3. Штамм бактерий Xanthomonas campestris ВКМ В-2249 Д - продуцент бактериолитического комплекса. 3. The strain of bacteria Xanthomonas campestris VKM B-2249 D - producing bacteriolytic complex.
RU2000129650/13A 2000-11-29 2000-11-29 Bacteriolytic complex, method of its preparing and strain for method realization RU2193063C2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
RU2000129650/13A RU2193063C2 (en) 2000-11-29 2000-11-29 Bacteriolytic complex, method of its preparing and strain for method realization
PCT/RU2001/000514 WO2002044352A1 (en) 2000-11-29 2001-11-28 Bacteriolytic complex, method for producing said complex and strain for carrying out said method.
KR1020027009664A KR20020073189A (en) 2000-11-29 2001-11-28 Bacteriolytic complex, method for producing said complex and strain for carrying out said method
US10/181,498 US7150985B2 (en) 2000-11-29 2001-11-28 Bacteriolytic complex, method for producing said complex and strain for carrying out said method
CNB018042473A CN1266271C (en) 2000-11-29 2001-11-28 Bacteriolytic complex, method for its production and strains used to achieve the method
US11/247,553 US20060051336A1 (en) 2000-11-29 2005-10-11 Bacteriolytic complex, method of its production, and strain for realization of the method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2000129650/13A RU2193063C2 (en) 2000-11-29 2000-11-29 Bacteriolytic complex, method of its preparing and strain for method realization

Publications (2)

Publication Number Publication Date
RU2000129650A true RU2000129650A (en) 2002-11-10
RU2193063C2 RU2193063C2 (en) 2002-11-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2000129650/13A RU2193063C2 (en) 2000-11-29 2000-11-29 Bacteriolytic complex, method of its preparing and strain for method realization

Country Status (5)

Country Link
US (2) US7150985B2 (en)
KR (1) KR20020073189A (en)
CN (1) CN1266271C (en)
RU (1) RU2193063C2 (en)
WO (1) WO2002044352A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2296576C2 (en) * 2005-05-18 2007-04-10 Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН AGENT OF BACTERICIDE ACTION IN RELATES TO VEGETATIVE AND SPORE CELLS Bacillus anthracis, METHOD FOR MALIGNANT ANTHRAX PROPHYLAXIS AND TREATMENT
RU2367469C2 (en) * 2007-05-23 2009-09-20 Закрытое акционерное общество "Биологические исследования и системы" Antibacterial and necrolytic local lisoamidase pharmaceutical composition
RU2407782C2 (en) * 2009-02-20 2010-12-27 Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, DNA FRAGMENT CODING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, AND METHOD OF OBTAINING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA
RU2408725C2 (en) * 2009-02-20 2011-01-10 Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI, DNA FRAGMENT, CODING LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI, AND METHOD FOR PRODUCTION OF LYTIC PROTEASE AlpB OF BACTERIUM Lysobacter sp XLI
WO2013087764A1 (en) * 2011-12-14 2013-06-20 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Process for degrading a biofilm on surfaces of objects
RU2744107C1 (en) * 2020-10-05 2021-03-02 Общество с ограниченной ответственностью «Газпромнефть Научно-Технический Центр» Xanthomonas fuscans bacterial strain - producer of xanthan gum

Family Cites Families (6)

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JPS5612435B1 (en) * 1971-03-29 1981-03-20
GB1353877A (en) 1972-10-12 1974-05-22 Nat Food Research Inst Directo Lytic enzymes
SU519470A1 (en) 1973-09-28 1976-06-30 Днепропетровский Ордена Трудового Красного Знамени Государственный Университет Им.300-Летия Воссоединения Украины С Россией Method for producing lytic enzymes
RU1549227C (en) 1984-10-04 1995-12-20 Институт биохимии и физиологии микроорганизмов РАН Method for producing a complex of lytic enzymes
SU1774658A1 (en) * 1990-11-05 1995-09-10 Институт Биохимии И Физиологии Микроорганизмов Ан Ссср Method for production of complex of lytic ferments
SU1755581A1 (en) * 1990-11-05 1995-08-20 Институт Биохимии И Физиологии Микроорганизмов Ан Ссср Method for isolation of complex of lytic ferments

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