RU2076732C1 - Preparation for sexual cycle regulation in animals - Google Patents

Abstract

FIELD: cattle breeding, veterinary science. SUBSTANCE: invention proposes the preparation FSH-super containing gonadotropic hormones at ratio of follicle-stimulating and luteinizing hormones = (1000-1500):1. EFFECT: enhanced effectiveness of regulation.

Description

 The invention relates to veterinary medicine and animal husbandry and for drugs for controlling the sexual cycle in a wide range of farm animals.

 In conditions of industrial keeping of animals, when individual control over animals is difficult, and sexual hunting and optimal insemination periods are often overlooked, functional disorders of the genital area often occur in animals. This leads to an increase in overshoots, low yield of offspring, early culling of animals and a decrease in the profitability of animal exploitation. In this regard, the control and management of the sexual cycle of animals are necessary in conditions of industrial production of livestock products.

 In the complex of measures aimed at solving this problem, hormonal regulation of the reproductive function of animals is of paramount importance and allows overcoming the low biological fecundity of cattle, inducing and synchronizing sexual hunting, regulating calving time, and shortening the service period.

 To this end, in veterinary and livestock practice, various preparations are used containing gonadotropic hormones of the anterior pituitary gland, which, when introduced into the body of animals, stimulate follicular growth, ovulation and the formation of yellow bodies. At the same time, the animals show signs of sexual hunting (B. P. Zavertyaev. Biotechnology in the reproduction and selection of cattle. L. Agropromizdat. Len. Department of 1989, p. 6 10).

 Hormonal gonadotropic drugs can be obtained either from the blood serum of mares, or from the pituitary gland of various farm animals.

 The drugs of the first group are more common. These include FFA (serum of mares), HFA (serum gonadotropin), as well as a number of preparations based on them - gravogormone, ovariotropin, follygon, malotropin, intergonan, etc. However, side effects may occur in animals. manifested in the form of anaphylaxis. High doses of drugs necessary to achieve a positive result cause the formation of large follicles with hemorrhages, multiple corpus luteum and an increase in the ovaries (5-7 times) with the formation of cysts. In addition, for all preparations based on the blood serum of mares, a long half-life is characteristic, which leads to the accumulation of exogenous hormones in the animal body. In the blood of animals, hormonal status changes, the ovaries are in a state of hyperstimulation, the mobility of the oviducts and uterus increases, which affects the normal development of gametes and the degree of fertility and causes a degenerative change in the embryos. In this regard, preparations of this group are not desirable for repeated use (Ernst L.K. Sergeev N.I. Transplantation of embryos of farm animals. M. VO Agropromizdat. 1989, p. 88 93).

 Pituitary hormonal preparations have a more favorable effect on the animal organism. They do not cause allergic reactions, have a short half-life, which allows them to be used for a long period of time without noticeable disturbances in the genital area of animals, while maintaining the sensitivity of the ovaries to gonadotropins (ibid., P. 94 95).

 However, the possibility of using pituitary gonadotropins in a production environment is limited due to the difficulty of obtaining pure preparations and their high cost, as well as the need for multiple treatments of animals, which significantly increases the labor costs of veterinary specialists (Polyanov N.I. Reproduction in industrial animal husbandry. M. Rosagropromizdat. 1990, p. 150). In this regard, preparations of this group are currently finding wider use for experimental purposes.

 Serum gonadotropin (HFA) was chosen as the reference drug (Sokolov V.D. Nozdrin G.A. Rybakov Yu. N. Medicines used in veterinary practice. Novosibirsk. Science. Sib. Department 1992, p. 146) widely used in practice for the treatment and prevention of various sexual disorders in animals. The drug is a light gray powder, soluble in water and isotonic solution. It induces hunting in 45 50 treated animals and increases fertility by 30 after artificial insemination.

 The basis of hormonal gonadotropic drugs, both serum and pituitary, is follicle-stimulating hormone (FSH). As a rule, preparations contain luteinizing hormone (LH) as an impurity to FSH, which is determined by the difficulty of separation and purification of FSH from LH.

 The presence of impurities complicates the standardization of drugs and the implementation of controlled (dosed) administration of hormones, which leads to their overdose and causes pathological changes in the gonads in animals (cystic changes in the ovaries, multiple corpus luteum, luteinization of follicles, etc.), which are explained by the loss of sensitivity of the receptors to gonadotropic hormones.

 Therefore, it is relevant to create gonadotropic drugs containing a minimum amount of impurities, which will improve the efficiency of using these drugs to regulate the sexual cycle and prevent infertility in animals.

 It is believed, for example, that the most effective effect on the animal reproductive system is exerted by FFA, in which the amount of FSH exceeds LH by 4-12 times. In the case of LH predominance, massive follicle luteinization is observed (follicle transformation into yellow bodies without prior ovulation). Attempts are also being made to use a serum gonadotropin preparation (HFA) with partially inactivated LH (2.5 times), resulting in an increase in the fertility of animals in induced hunting, which contributed to a reduction in the service period (V. Darovskikh et al. Biological properties of HFA preparations and pituitary FSH. Proceedings of the Institute of Physiology and Nutrition of Animals. 1991 (1990), N 54, p. 35 39).

 The technical effect of the invention is to increase the effectiveness of the drug.

 The technical effect is achieved using a preparation containing gonadotropic hormones of the anterior pituitary gland of pigs with a ratio of FSH to LH equal to 1000 1500 units. FSH / 1 unit LH (FSH LH (1000 1500) 1).

 A high degree of chromatographic purification allows one to obtain a preparation with an FSH / LH ratio of (1000 1500) 1, which is characterized by a prolonged action (it remains in the blood for 48 72 hours, unlike other pituitary gonadotropins), high physiological activity at low doses and a single administration and the absence of negative effects on the animal organism. The proposed drug eliminates luteal aggression of the ovaries, leading to the formation of follicular cysts.

 The preparation is an amorphous powder of grayish-white or grayish-yellow color, odorless, readily soluble in physiological saline and distilled water. Apply as a solution.

 Introduction to the agricultural practice of the proposed drug will solve the raw material problem of obtaining gonadotropic drugs, because more affordable raw materials (pituitary glands of pigs) are used to obtain them compared to the blood of mares.

 The proposed drug was tested in production conditions on animals with various disorders of the genital area (hypofunction, persistent corpus luteum, anovulatory syndrome, etc.). Control and experimental groups of animals were selected on the basis of analogues. Animals of both groups were in the same conditions of feeding and keeping.

 The effectiveness of the drug was evaluated by the number of animals that came into the hunt and became pregnant after artificial insemination.

 Example. At the Plemzavod Ardabyevsky farm of the Ryazan region (black and white cows), heifers of 18 19 months of age with a hypofunctional state of the reproductive system were selected. Control animals (25 animals) were injected with HFA in a dose of 3000 IU. The experimental group (28 goals) was administered FSH-super at 100 IE.

 Among the control animals, 20 animals showed hunting, and became 12 pregnant. In the experimental group, 70 animals came to hunting and became pregnant.

 According to a similar scheme, the proposed drug was tested in the GP “Karavaevo” of the Kostroma region on cows of the Kostroma breed to stimulate reproductive function. In the control group, hunting was induced in 55 animals, in the experimental group in 87 animals.

In the Smena GPPZ Zagorsky district of the Moscow Region, animals (Aikshir breed) with long post-calving anestrus or inseminated many times to no avail were selected. The cows of the control group (28 animals) were treated with HFA (3000 IU), and the experimental group (29 animals) was twice FSH super after 12 hours at 100 IE. In the first group, hunting was shown by 40 cows with a pregnancy of 20 In the second group, 73 with a pregnancy of 70
In the Forestry Glades GPZ of the Pushkin District of the Moscow Region, tests were carried out on cows of the Kholmogorsk breed. One group of cows (30 animals) had a hypofunctional ovarian condition. Animals of another group (40 goals) - the normal state of the reproductive system. Half of the animals of each group were injected with HFA in a dose of 3000 IU, and the other half of the proposed drug in a dose of 100 IE. Among animals suffering from disorders of the reproductive system, in the first case 33 came to the hunt and in the second 67 In healthy animals, the first treatment option caused hunting in 75 and the second in 100
Another group of animals (29 animals), which had a prolonged anestrus after calving (40-60 days), FSH-super was administered twice with an interval of 5 days at 100 IE. Signs of hunting were observed in 70 animals.

 Tests have shown that the proposed drug induces sexual hunting in cows, which occurs simultaneously with a gap of 1 2 days, increases fertility in experimental animals by 50, which significantly reduces the service period in the absence of adverse reactions.

 At the Gornyak gas processing plant in the Rostov Region, the drug was used to eliminate mass infertility and shorten the service period.

 For this purpose, calving cows were administered FSH super once at a dose of 300,400 IE 12 days after calving or earlier. On day 2–6 after administration of the drug, 70 animals reacted by hunting and ovulation. Insemination was carried out in the next hunt due to incomplete genital involution on days 14-22 after calving. The service period in the herd was reduced by 40 45 days compared with HSC in the absence of postpartum depression of the ovaries.

 The drug was also used in the treatment of infertility in sows, which depends on the term for weaning piglets. Acyclia attacks lactational stress after weaning piglets.

 At the Kuznetsovsk pig-breeding complex of the Moscow region, an experimental group of sows (50 animals) was administered once at a dose of 100 IE at the proposed preparation 3–4 days after weaning. The control group of animals was injected with HFA in 2000 IE. In the experimental group, sow fertility increased by 5.1 and fecundity by 1.7 pigs.

 At the Vladimir complex, FSH-super was used in different doses to induce sexual hunting in sows after weaning piglets. The control group of animals was treated with HFA in a dose of 2000 IE (66 animals). FSH-super was administered to experimental animals as follows: twice after 12 hours at 50 IE (84 heads), twice after 12 hours at 100 IE (36 goals) and once at 200 IE (37 goals).

 In control, 72.7 sows came into the hunt. Among the experimental group, 94.4 sows came into the hunt. The best was the option with a double introduction of the proposed drug after 12 hours at 100 IE.

 The drug was also tested in fur farming (fur farm "Pushkinsky" in the Moscow region). Ferret females were chosen as a model. The selected animals for further economic use were not suitable. The controls were untreated animals. FSH-super were administered to the experimenters either twice 4 times IE 4 times, or 4 times IE 3 times, and 4 times IE once. Multiplicity of processing did not affect the results of the experiment. A single injection of FSH super at a dose of 4 IE caused among the dysfunctional livestock of female ferret females an increase in the number of pups by 55.1, increasing fertility by 2.2 puppies.

 FSH super was also used to induce superovulation in donor cows at a total dose of 1000 IE. Compared with the known drug, the yield of full-fledged embryos increases by 20 30

Claims (1)

 A preparation for controlling the animal reproductive cycle containing follicle-stimulating and luteinizing hormones, characterized in that the ratio of follicle-stimulating and luteinizing hormones is 1000 1500 1.