RU2057175C1 - Nutrient medium for microorganism growing - Google Patents

Abstract

FIELD: microbiological industry. SUBSTANCE: liquid phase of hydrolytic lignin press drying is neutralized with milk of lime and ammoniacal water and gypsum formed is separated. Required quantity of mineral salts containing nitrogen, phosphorus and potassium is added to filtrate, diluted with water. Yeast Candida is sown and grown at 30-40 C under continuous aeration. EFFECT: enhanced quality of medium. 2 tbl

Description

 The invention relates to the microbiological industry, and in particular to nutrient media for growing microorganisms, in particular fodder yeast.

Known nutrient medium for growing fodder yeast, containing a hydrolyzate of plant materials as a carbon source and sources of mineral salts [1]
Also known is a nutrient medium for the cultivation of fodder yeast, including a hydrolyzate of plant materials, sources of nitrogen, phosphorus, potassium. As sources of carbon for their life, yeast use monosaccharides and organic acids, the content of which in hydrolysates does not exceed 0.2-0.4% [2] (prototype).

 The disadvantage of the above nutrient media is the relatively low biomass yields of 48-55% of the RS. This is due to the fact that, as a carbon source, these nutrient media contain mainly only monosaccharides.

 The aim of the invention is to increase the yield of biomass of microorganisms.

 The goal is achieved by a nutrient medium of known composition containing a carbon source in the form of a hydrolyzate of plant materials, a source of mineral nitrogen, phosphorus, potassium ions, in which the liquid phase of press drying of hydrolysis lignin containing, by weight, is used as a carbon source. monosaccharides (in terms of PB) 1.0-7.0; organic acids 0.8-3.2 and ligno-humic substances 0.3-1.6, water the rest in the following ratio of components, wt.

Source of mineral
nitrogen (in terms of
nitrogen) 0.07-0.49
Potassium ions 0.025-0.175
Phosphorus (in terms of
P ₂ O ₅ ) 0.3-1.6
The liquid phase of the press
hydrolysis drying
lignin rest
Comparative analysis with the prototype allows us to conclude that the claimed nutrient medium differs from the known ones using hydrolysis lignin as a hydrolyzate of the liquid phase of the press drying of the press, as well as a new quantitative ratio of the ingredients of the composition.

As studies have shown, after percolation hydrolysis of plant materials, the non-hydrolyzed part of the product contains hardly hydrolyzable polysaccharides. When the hydrolysis lignin is press-dried under conditions of uniformly increasing pressure in the presence of H ₂ SO ₄ , additional hydrolysis of the hardly hydrolyzable polysaccharides occurs. This is confirmed by a decrease in the content of hardly hydrolyzable substances in squeezed lignin. In addition, there is also a partial destruction of the lignocellulosic complex with the formation of organic acids, an additional source of carbon nutrition of yeast. Thus, as a result of deeper hydrolysis of the lignocellulosic material, the liquid phase will be enriched with both monosaccharides (glucose) and organic acids.

 The use of this liquid phase as a source of carbon with high nutritional value also requires more nutrient salts (nitrogen, phosphorus, potassium), which has changed the quantitative composition of the claimed nutrient medium.

 The following are examples of the preparation of a culture medium for the cultivation of yeast.

From the lignin of the Tavda hydrolysis plant with a moisture content of 66.1% acidity of 2.6%, the content of hardly hydrolyzable substances 21.5% during the drying of the lignin by the press method (application N 4777935/06, positive decision of 04/12/1990) with a uniformly increasing pressure from 10 MPa up to 50 MPa receive the liquid phase of the extraction of lignin. The resulting solution is neutralized with lime milk and ammonia water to pH 4.2-4.4 at 80-85 ^{° C} and blowing air, and then formed gypsum is separated by sedimentation or filtration. Mineral salts containing nitrogen, phosphorus and potassium are added to the filtrate in the required amount. Diammophos was used as a source of nitrogen and phosphorus, and nitrogen introduced into the solution when neutralizing the liquid phase with ammonia water was taken into account. Potassium chloride was used as a source of potassium.

The resulting mash was diluted to a working concentration CM and transmitting on a biochemical processing, such as growing feed yeast Candida scottii Toole-6 at a temperature of 30-40 ^{° C,} pH 4.2-4.4, and a continuous aeration.

 In the table. Figure 2 shows the cultivation of yeast Candida scottii Thul-6 on substrates obtained from the liquid phase of the extraction of lignin. As can be seen from the data in table 2 from 1 l of the liquid phase of the extraction, you can additionally obtain 45-47 g of absolutely dry yeast, and the yield of yeast biomass on the specified substrate increases by 26.8-56.3% compared with the prototype.

Claims (1)

Nutrient medium for growing microorganisms containing a carbon source in the form of a hydrolyzate of plant materials, a source of mineral nitrogen, phosphorus, potassium ions, characterized in that, in order to increase the yield of microorganism biomass, it contains a liquid phase of press drying of hydrolysis lignin containing , wt.%:
Monosaccharides (in terms of RV) - 1 - 7
Organic acids - 0.8 - 3.2
Ligno-humic substances - 0.3 - 1.6
Water - Else
in the following ratio of components, wt.%:
Mineral nitrogen source (in terms of nitrogen) - 0.07 - 0.49
Potassium ions - 0.025 - 0.175
Phosphorus (in terms of P ₂ O ₅ ) - 0.3 - 1.6
Hydrolysis Lignin Press Drying Liquid Phase - Else

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