RU2052266C1 - Method of cow hepatosis treatment - Google Patents

Abstract

FIELD: veterinary. SUBSTANCE: bioactive substance eracond is administrated intramuscularly to animals. Eracond is plant preparation containing vegetable condensed extract, for example, herb or hay of alfalfa and containing dissolved metal salts, mg/kg plant mass: Mo 8.0; Ba 10.0; Pb 20.0; V 1.0; Cr 0.5; Zn 200.0; Fe 300.0; Sn 40.0. Eracond (20% solution) is administrated intramuscularly at the dose 55 ml per animal once for 24 hr every day for 3 days and after 5 days interruption - repeatedly, orally as 10% solution at the dose 220 ml per animal, once for 24 hr every day and after 5 days interruption - repeatedly. EFFECT: increased effectiveness of treatment. 1 tbl

Description

 The invention relates to veterinary medicine and can be used to treat hepatosis of cows.

 Toxic liver dystrophy hepatosis (Dystrophia hepatioxica Hepatosis) is characterized by dystrophic damage to the liver without a pronounced mesenchymal cell reaction, accompanied by phenomena of general toxicosis. Mostly lactating cows, gilts and calves are sick during weaning and fattening.

 The most common and immediate cause of the disease is the accumulation on the farm or eating grains damaged by toxic mushrooms, spoiled silage, bard, pulp, animal feed, and poisonous plants on the pasture. The disease can also occur when animals eat mineral fertilizers and treated with herbicides, overdoses of cattle (cattle) urea. Symptoms of liver dystrophy are observed in cattle with ketosis. Various factors reducing the body's resistance contribute to the disease: hypovitaminosis, mineral starvation, lack of exercise, irregular feeding, and gastrointestinal infections.

 The diagnosis is usually made on the basis of an anamnesis, clinical presentation and autopsy.

 In the treatment of hepatosis, toxic or suspected feed toxicity is excluded from the diet of animals. A diet of easily digestible benign feed is prescribed and the rate of concentrates is reduced accordingly. A sick cattle are washed with scar water with warm water, put deep cleansing enemas and prescribe laxatives. To bind toxins and absorb excess gases, milk, aqueous suspensions of activated charcoal or charcoal are introduced inside the probe. To stimulate peristalsis and secretory function of the intestine, Karlovy Vary salt, sodium or magnesium sulfate are indicated in small doses. With the development of intoxication, glucose with ascorbic acid and cardiac agents are administered intravenously. For 2-3 weeks after clinical recovery, the animals are kept on a sparing diet. Methionine and vitamins are also prescribed (see Veterinary formulation with the basics of therapy and prevention. M. Agropromizdat, 1988. S. 167-169).

For the treatment of the liver, cyanocobalamin (Cyanocabalaminum) vitamin B ₁₂ (Vitaminum B ₁₂ ) is used, which has high biological activity and has a beneficial effect on liver function. Oxycobalamin (Oxycobalaminum) and cobamamide (Cobamamidum) are close to it.

 Vitohepat (Vitohepatum), sirepar (Surepar), calcium chloride (Calcii chloridum), potassium orotate (Kalii arotas) are also used. Ascorbic acid (Acidum ascoridum) can be distinguished from plant products. It is found in rose hips, cabbage, lemon, orange, horseradish, fruits, berries, needles (see Mashkovsky MD Medicines. M. Medicine, 1989, part 2, p. 130).

 As a drug that stimulates metabolic processes, glucose (Glucosum) can be distinguished. With the introduction of hypertonic (10-40%) solutions into the vein, metabolic processes increase, the antitoxic function of the liver improves.

 The liver is involved to one degree or another in all metabolic processes. Therefore, when its function is disturbed, the regulation of metabolic reactions changes, the biochemical parameters of the blood change, the activity of cellular and humoral factors of resistance of the animal organism decreases.

 The considered drugs for the treatment of toxic liver dystrophy are either not effective enough, or their use leads to a change in the morphological composition of the blood.

 The proposed method for the treatment of hepatosis of cows is implemented as follows.

 The Trinity Veterinary Institute together with the Southern Ural collective farm of the Troitsky district of the Chelyabinsk region conducted a scientific and production experiment.

 Five cow groups were selected according to the principle of analogues, ten cows in each group.

 In cows of groups 1, 2, 3 (experimental) and 4 groups (control), hepatosis was established on the basis of clinical signs, biochemical blood tests and a test with sulfuric acid copper according to V.S. Postnikov. Cows of group 5 are healthy (control).

In the blood of cows with impaired functional state of the liver (groups 1-4), the level of carotene (0.32 ± 0.04-0.39 ± 0.04 mg%), glucose (37.86 ± 2.13-39, 96 ± 2.38 mg%), phosphorus (3.1 ± 0.13-3.94 ± 0.19 mg%), calcium (8.9 ± 0.33-9.46 ± 0.41 mg%) sodium (252.3 ± 20.02-312.1 ± 4.46 mg%), potassium (14.4 ± 1.25-15.92 ± 13.6 mg%), reserve alkalinity (30.08 ± 1.11-37.64 ± 3.13% CO ₃ ) and an increased content of total protein (9.03 ± 0.31-10.0 ± 1.2 g%), copper (122.8 ± 7.12 -144.6 ± 2.71 μg%). At the same time, these indicators in healthy cows of group 5 were within normal limits.

 In patients with hepatosis of cows, in contrast to healthy animals, the following changes were noted: a slightly depressed general condition, the hair was ruffled, matte, and skin elasticity was reduced. The appetite is lowered, the chewing gum is sluggish, short. Hypo- or atony of the scar, an increase in the area of hepatic dullness, pain on palpation of the liver was noted.

 The test with copper sulfate according to V.S. Postnikov was negative (-) in group 5, positive (++) and sharply positive (+++) in the remaining groups.

 The cows of the experimental groups were injected with a heracond according to a certain scheme. The drug Erakond is an aqueous extract of alfalfa grass (hay) obtained by treatment with an extractant containing a mixture of trace elements, mg / kg of plant mass: Mo 8.0; Ba 10.0; Pb 20.0; Co 1.05; V 1.0; Cz 0.5; Zn 200.0; Fe 300.0; Sn 40.0.

 The method of preparation and composition of the heraconda is as follows.

 Prepare a solution of salts of trace elements based on distilled water vapor condensate, including Mo 8.0 mg, Ba 10.0 mg, Pb 20.0 mg, Co 1.05 mg, V 1.0 mg, Cz 0.5 mg, Zn 200, 0 mg, Fe 300.0 mg, Sn 40.0 mg.

Grass (hay) alfalfa ground and placed in an autoclave, pour the prepared solution in a ratio of 1: 5, the autoclave was sealed, heating is performed to a temperature of ¹²⁰ ° C and held for 10 minutes. The autoclave is depressurized, the primary extract is poured into the storage tank, and the raffinate remaining in the autoclave is again poured with the extracting mixture in the same ratio and heated to a temperature of 180 ° C ^for 10 minutes. The secondary extract is poured into a storage tank and mixed with the primary extract. The resulting mixture was evaporated until numerous bubbles appeared on the mirror. Then the extract is filtered through a cotton-gauze filter. The resulting filtrate was placed on a baking sheet covered with a polyethylene film, and continue to evaporate in an oven at ⁶⁰ ° C until the mass of plasticizing the entire thickness poured onto a baking extract. The resulting mass is formed into a lump for future use or storage.

 A sterile 10-20% aqueous solution of Heraconda on distilled water was prepared from the plasticizing mass. The solution was filtered through several layers of gauze and used immediately after preparation (it is possible to ampoule the solution for long-term storage).

 In group 1, intravenously 2 times with an interval of 1 day (10% solution at a dose of 110 ml per head) and after a 5-day break again.

 In the second group, intramuscularly (20% aqueous solution, 55 ml per 1 cow 1 time per day) and in the third group inside (10% aqueous solution, 220 ml per 1 head, 1 time per day) daily for 3 days and after a 5-day break again.

Subsequent blood tests showed that in the blood of cows of the experimental groups, the content of carotene (0.45 ± 0.07-0.50 ± 0.05 mg%), glucose (48.3 ± 2.08-52.5 ± 2, 34 mg%), phosphorus (5.28 ± 0.68-6.36 ± 0.41 mg%), calcium (9.58 ± 0.32-10.04 ± 0.27 mg%), sodium (322 , 4 ± 6.82-331.2 ± 11.93 mg%), potassium (17.26 ± 1.63-18.80 ± 0.90 mg%), total protein (6.8 ± 0.27- 7.28 ± 1.02 g%), the level of reserve alkalinity (46.86 ± 1.07-50.36 ± 2.19 vol% CO ₂ ), while the condition of the animals of group 4 worsened.

 The level of these indicators in the blood of healthy cows of group 5 slightly decreased, but continued to remain within the normal range, except for the carotene content (0.26 ± 0.07 mg%).

 In the course of the experiment, it was found that the introduction of intramuscularly and orally (see table) is an effective way of using heraconda.

 Thus, the results show that under the influence of heraconda, the clinical status of cows with hepatosis of cows has significantly improved and the functional state of the liver has normalized. At the same time, no changes in the morphological composition of blood under the influence of heraconda were found. The digestion of animals was normalized. Liver soreness on palpation disappeared.

Claims (1)

 METHOD FOR TREATING COW HEPATOSIS, including the introduction of a biologically active substance, characterized in that a plant alfalfa extract obtained by treatment with an extractant containing a mixture of trace elements, mg / kg of plant mass, is used as a biologically active substance: Mo - 8; Ba - 10.0; Pb - 20; Co - 1.05; V is 1; Cz - 0.5; Zn - 200; Fe - 300; Sn - 40, which is administered in the form of a 20% aqueous solution intramuscularly at a dose of 55 ml per cow once a day for three days, after a five-day break, the course is repeated or administered in a dose of 220% in the form of a 10% aqueous solution ml per cow once a day for three days, and after a five-day break, the course is repeated.

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