RU190718U1 - DISPOSABLE DEVICE FOR FIXING OF SMALL LABORATORY ANIMALS FOR PHOTODYNAMIC THERAPY - Google Patents

Abstract

The invention relates to the field of experimental biology, namely, disposable devices for fixing small laboratory animals during photodynamic therapy. The disposable device consists of a base, an upper screen with a hole for spot irradiation, and five fixing devices, the base being made of corrugated cardboard of a rectangular shape, one fixing device at the edges of each corner consisting of two parts: a rubber cord in a textile braid and a plastic during fixation, forming a closed loop, with the upper screen made of black cardboard according to the size of the base, which covers the laboratory animal and is fixed to the base using a locking device that connects the base and the top screen. One of the four clamps on the base is positioned so that the irradiated limb of the laboratory animal is extended, and this clamp can be located both under the right and under the left irradiated limb. The opening of the upper screen corresponds to the place of the necessary zone of radiation of the laboratory animal. The use of the device allows to achieve reliable fixation of non-narcotized small laboratory rodents for a long time in the position necessary for photodynamic therapy, without adversely affecting the regional blood circulation in its extremities, and also protecting the body of mice from unnecessary radiation and hyperthermia outside the study areas and providing the necessary ventilation. The proposed device is industrially applicable, as it can be produced in the form of disposable sets for experimental biology. The device is simple to manufacture and does not require large expenditures. 6 Il.

Description

The invention relates to the field of experimental biology, namely, disposable devices for fixing small laboratory animals during photodynamic therapy.

During the sessions of laser irradiation, it is important to have a clear and long-term fixation of anesthetized and non-narcotized animals. At the same time, it is necessary to ensure the most comfortable, without pain and pinching of the vessels, the form of fixation. Mice should not be under severe stress or be in an unnatural position for a long time, as this can lead to illness and death of the animal. Tumors for photodynamic therapy are usually transplanted to the thighs of mice, therefore, it is particularly important to isolate and clearly fix this particular zone, with the screening of the rest of the body surface from radiation.

To ensure such conditions in laboratory practice using different fixing devices.

A device for careful fixation in a stationary state of small laboratory animals (RU 2524251 C1), contains a body from the “stenoflex” with a loading opening and is equipped with a tripod, while the device uses a piece of a cylindrical soft elastic tube with two stainless steel crimp clamps as the body steel with a screw, transmitting the force of the worm type.

However, a disadvantage of the known device for careful fixation is the excessive rigidity of the materials used, namely the metal crimped clamps and the body of the "stenoflex", which make it difficult to control the body position of the laboratory animal during fixation. At the same time, metal clamps require a tool to work with them (a screwdriver or a wrench) and do not allow fixing quickly, and a tripod made of wood cannot be fully sterilized by chemical means used in the operating room. In addition, the material from which the instrument case is made does not allow air to pass through and is unable to absorb liquid, which under conditions of prolonged manipulation of laboratory animals may have undesirable consequences (asphyxiation, contamination of the surgical field with biological fluids, etc.).

There is a method of fixing small laboratory animals with adhesive tape on the limbs, torso and head, in a supine position on the operating table, which consists in placing an anesthetized small laboratory animal on a device containing a base with stand supports and guides that follow the contours of a rat placed on the back, with outstretched front and hind legs (RU 2303421 C1).

The disadvantage of this method of fixation is that it is obligatory for it to anesthetize the biomodel (rat), which is unacceptable for simple manipulations that do not require general analgesia. In addition, this method is quite time-consuming and difficult to perform by one person. In the absence of an assistant, these defects significantly increase the time of manipulations, which, in turn, require large doses of narcotic drugs. In addition, the presented method cannot be used for fixing laboratory mice and does not allow changing the position of the animal, which makes it difficult or impossible to use a number of widely used techniques.

Known methods of fixation, where as the housings of devices use segments of the oxygen hose (RU 2521622 C1) or cylinders cut from a polyethylene terephthalate bottle with a volume of 0.375-0.5 liters, in which 4/5 of the bottle is circular across the cross-section of the bottle 5 cm from the bottom, an incision is made, which forms a rat inlet opening when the bottom is folded (RU 2461368 C1).

However, the known methods do not allow to clearly fix the hind limb of the mouse and are not suitable for photodynamic therapy.

There is a method of fixing small laboratory animals (RU 2590637 C1), characterized in that the head, torso and extremities of a non-narcotized animal are wrapped in one and a half turn with a polyurethane foam sheet, stretching it in the central part, to ensure that the material fits snugly to the entire body surface of the laboratory animal, and the loose the edges of the leaf in front of the nose and at the base of the tail are closed and fixed.

However, the known method also does not allow for a clearly and non-traumatic fixation of the hind limb for photodynamic therapy, since the mouse can still jerk with its hind limb and shoot down the irradiation settings.

The technical result of the claimed utility model is the creation of a disposable device for fixing small laboratory animals for photodynamic therapy, allowing you to easily and quickly access the laboratory animal's thigh and irradiate the immobilized area under conditions that maximize the negative factors of long-term immobilization.

This technical result is solved in that it consists of a base, an upper screen with a hole for conducting point irradiation and five fixing devices, the base being made from a rectangular corrugated cardboard with one fixing device at the edges of each corner consisting of two parts: a rubber cord in textile braid and plastic retainer, which form a closed loop during fixation, with the upper screen made of black cardboard according to the size of the base with which the laboratory is covered alive peppermint and fixed to the base by means of the locking device connecting the base and the top screen. One of the four clamps on the base is positioned so that the irradiated limb of the laboratory animal is extended, and this clamp can be located both under the right and under the left irradiated limb. The opening of the upper screen corresponds to the place of the necessary zone of irradiation of the laboratory animal.

The utility model is illustrated with a detailed description and illustrations, which depict:

FIG. 1 - a) the appearance of the device for fixing small laboratory animals for photodynamic therapy; 1 - base; 2 - screen for irradiation with a hole - 6; 3 - locking device; 4 - rubber cord in textile braid; 5 - plastic retainer; b) the device is assembled.

FIG. 2 - the dorsal side of the device; 1 - base; 4 - the ends of the rubber cord; 5 - plastic retainer.

FIG. 3a, 6 is a device diagram with dimensions for fixing a laboratory animal (mouse).

FIG. 4 shows an example of fixing a laboratory animal (mouse) without an upper shielding part; view from above.

FIG. 5 shows an example of fixing a laboratory animal (mouse) without an upper shielding part; side view.

FIG. 6 shows an example of fixing a laboratory animal (mouse) with an upper shielding part; view from above.

FIG. 7 is an example of fixing a laboratory animal (mouse) with an upper shielding part; side view.

A device for fixing small laboratory animals for photo dynamic therapy (Fig. 1, Fig. 2) consists of a base 1, an upper screen 2 with an opening 6 for photodynamic therapy, and five fixing devices 3, the base 1 being made of a rectangular corrugated cardboard , at the edges of each corner there are fixing devices 3, consisting of two parts: a rubber cord in a textile braid 4 and a plastic fixer 5, which form a closed loop during fixation. The upper screen 2 is made of black cardboard according to the size of the base 1, the upper screen 2 being fixed to the laboratory animal and the base 1 by a device 3 connecting the base and the upper screen.

A device for fixing small laboratory animals for photodynamic therapy works as follows.

The non-narcotized laboratory animal (mouse) is placed on a rectangular base of corrugated cardboard 1 and its limbs are fixed with the help of a fixing device 3 consisting of a rubber cord with textile braid 4 providing soft, non-traumatic but complete fixation. Loops tighten the bottom of the cardboard base 1 and fixed with plastic clips 5.

The laboratory animal is covered with an upper screen 2 made of black cardboard with a technological opening 6, specified in the diameter experiment, under the trained extremity of the mouse. The upper screen 2 to the laboratory animal and the base 1 is fixed using the device 3.

The material used provides normal ventilation, eliminates overheating of the animal, absorbs biological fluids and natural excretions. The degree of fixation of the animal is regulated by the tension of the rubber cord. All materials are available, economical and easily recyclable.

An example of execution.

For the experiment, the device was made according to the dimensions indicated in figure 3 (a, b). Unarcotized white mouse, weighing 20 g, female. The mouse was placed on a cardboard base 1 (Fig. 4), and with large laboratory tweezers they gently grabbed the legs and pulled them into loops of rubber cord 4, after which the loop was tightened from below with a plastic retainer 5. on which the irradiation was planned, a gauze pad was enclosed to further level the surface under the laser irradiation (Fig. 5). On top of the back, the mouse was covered with an upper screen 2 made of black cardboard, so that the hole 6 corresponded to the left hip irradiation zone (Fig. 6). The hole diameter was 1.5 cm. The base 1, with the mouse located on it and covered with the upper screen 2, was fixed with the help of a fixing device 3 (Fig. 7). At the next stage, the fixed animal was placed in the laser setup and irradiated with a laser for 5 minutes. At the end of the manipulation, the animal was released, by loosening the fixing rubber cords.

The use of the device allows to achieve reliable fixation of non-narcotized small laboratory rodents for a long time in the position required for photodynamic therapy, without adversely affecting the regional circulation in its limbs, eliminating overheating, and protecting the body of mice from unnecessary exposure outside the study areas.

The proposed device allows you to:

- to simplify the method of fixation, due to the use in its implementation of simple clamps;

- to increase the reliability of fixation due to tight fit with rubber bands with textile sheathing of the legs of the animal;

- to manipulate non-narcotized animals;

- to eliminate possible injuries of the limbs on the sharp edges of the base, due to the soft corrugated cardboard;

- reduce stress in laboratory animals due to the exclusion of uncomfortable postures;

- protect the body of mice from unnecessary exposure outside the study areas.

- ensure good ventilation and eliminate overheating during procedures.

The proposed device is industrially applicable, as it can be produced in the form of disposable sets for experimental biology. The device is simple to manufacture and does not require large expenditures.

Claims (3)

1. A disposable device for fixing small laboratory animals for photodynamic therapy, characterized by the fact that it consists of a base, an upper screen with a hole for conducting point irradiation, and five fixing devices, the base being made of a rectangular corrugated cardboard, along the edges of each corner one fixing device consisting of two parts: a rubber cord in a textile braid and a plastic retainer, which form a closed loop during fixation, with the upper ec en is made of black cardboard base size, which harbor laboratory animal and secured to the base by means of the locking device connecting the base and the top screen. 2. The device according to claim 1, characterized in that one of the four fixatives on the base is positioned so that the irradiated limb of the laboratory animal is extended, and this fixative can be located both under the right and under the left irradiated limb. 3. The device according to PP. 1, 2, characterized in that the opening of the upper screen corresponds to the place of the necessary zone of irradiation of the laboratory animal.

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