PT101813A - Use of di-mannosyl-di-myo-inositol phosphate and 1,3-di- myo-inositol phosphate in the thermal stabilization and osmoprotection of cell components and cells and their protection against dehydration - Google Patents
Use of di-mannosyl-di-myo-inositol phosphate and 1,3-di- myo-inositol phosphate in the thermal stabilization and osmoprotection of cell components and cells and their protection against dehydration Download PDFInfo
- Publication number
- PT101813A PT101813A PT10181396A PT10181396A PT101813A PT 101813 A PT101813 A PT 101813A PT 10181396 A PT10181396 A PT 10181396A PT 10181396 A PT10181396 A PT 10181396A PT 101813 A PT101813 A PT 101813A
- Authority
- PT
- Portugal
- Prior art keywords
- myo
- inositol phosphate
- mannosyl
- inositol
- drying
- Prior art date
Links
- 229910019142 PO4 Inorganic materials 0.000 title abstract 10
- 229960000367 inositol Drugs 0.000 title abstract 10
- 239000010452 phosphate Substances 0.000 title abstract 9
- 210000004027 cell Anatomy 0.000 title 1
- 210000003850 cellular structure Anatomy 0.000 title 1
- 230000018044 dehydration Effects 0.000 title 1
- 238000006297 dehydration reaction Methods 0.000 title 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 title 1
- 230000006641 stabilisation Effects 0.000 title 1
- 238000011105 stabilization Methods 0.000 title 1
- 235000021317 phosphate Nutrition 0.000 abstract 8
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000003599 detergent Substances 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- 238000009825 accumulation Methods 0.000 abstract 1
- 239000002671 adjuvant Substances 0.000 abstract 1
- 239000011942 biocatalyst Substances 0.000 abstract 1
- 230000031018 biological processes and functions Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000002502 liposome Substances 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 239000007800 oxidant agent Substances 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This invention relates to a biological process for the biosynthesis of two intracellular solutes identified as di-mannosyl-di-myo-inositol phosphates and 1,3'-di-myo- inositol phosphate. These have been identified in the hypothermophilic bacteria Thermatoga maritima and Thermatoga neapolitana (growth Topt 80 degree C) during growth at supraoptimal temperatures in complex medium. The environmental conditions necessary for their accumulation in high concentrations indicate that these new compatible solutes can have an effect in protecting macromolecules (specifically biocatalysts) and cells against operations involving heating, drying or freezing. Di-mannosyl-di-myo-inositol phosphate and 1,3'-di-myo- inositol phosphate can be used in enzyme catalysed processes at high temperatures or when these are performed in the presence of chemical denaturing agents such as detergents, organic solvents, oxidizing agents or caotropic agents such as urea or guanidine chloride with the intention of stabilizing their activity over the short and long term. Di-mannosyl-di-myo-inositol phosphate and 1,3'-di-myo- inositol phosphate can also be used to stabilize liposomes and other membrane systems used in the microencapsulation of drugs and other compounds used in the pharmaceutical, cosmetics, detergents and other industries. <IMAGE> Di-mannosyl-di-myo-inositol phosphate and 1,3'-di-myo- inositol phosphate can also be used during the drying of proteins and whole cells for transport and prolonged conservation and also as adjuvants for the preservation of microorganism strains or animal and plant cell lines, as well as perfused organs by freeze-drying or at low temperatures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT10181396A PT101813B (en) | 1996-01-11 | 1996-01-11 | USE OF DI-MANOSIL-DI-MIO-INOSITOL AND PHOSPHATE OF 1,3-DI-MIO-INOSITOL PHOSPHATE IN THERMOESTABILIZATION, OSMOPROTECTION AND PROTECTION AGAINST DEHYDRATION OF CELL COMPONENTS AND CELLS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT10181396A PT101813B (en) | 1996-01-11 | 1996-01-11 | USE OF DI-MANOSIL-DI-MIO-INOSITOL AND PHOSPHATE OF 1,3-DI-MIO-INOSITOL PHOSPHATE IN THERMOESTABILIZATION, OSMOPROTECTION AND PROTECTION AGAINST DEHYDRATION OF CELL COMPONENTS AND CELLS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT101813A true PT101813A (en) | 1997-08-29 |
| PT101813B PT101813B (en) | 1998-12-31 |
Family
ID=20085562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT10181396A PT101813B (en) | 1996-01-11 | 1996-01-11 | USE OF DI-MANOSIL-DI-MIO-INOSITOL AND PHOSPHATE OF 1,3-DI-MIO-INOSITOL PHOSPHATE IN THERMOESTABILIZATION, OSMOPROTECTION AND PROTECTION AGAINST DEHYDRATION OF CELL COMPONENTS AND CELLS |
Country Status (1)
| Country | Link |
|---|---|
| PT (1) | PT101813B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001076572A3 (en) * | 2000-04-12 | 2002-04-11 | Bitop Gmbh | Use of compatible solutes as substances having free radical scavenging properties |
| WO2002015868A3 (en) * | 2000-08-18 | 2002-07-18 | Bitop Gmbh | Cosmetic formulation containing at least one di-sugar alcohol phosphate of c3 to c6 sugar alcohols |
| CN104450794A (en) * | 2013-09-25 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Synergist for improving yield of alcohol |
-
1996
- 1996-01-11 PT PT10181396A patent/PT101813B/en active IP Right Grant
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001076572A3 (en) * | 2000-04-12 | 2002-04-11 | Bitop Gmbh | Use of compatible solutes as substances having free radical scavenging properties |
| WO2002015868A3 (en) * | 2000-08-18 | 2002-07-18 | Bitop Gmbh | Cosmetic formulation containing at least one di-sugar alcohol phosphate of c3 to c6 sugar alcohols |
| CN104450794A (en) * | 2013-09-25 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Synergist for improving yield of alcohol |
Also Published As
| Publication number | Publication date |
|---|---|
| PT101813B (en) | 1998-12-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Patent granted, date of granting |
Effective date: 19980902 |