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PL443827A1 - Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application - Google Patents

Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application

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Publication number
PL443827A1
PL443827A1 PL443827A PL44382723A PL443827A1 PL 443827 A1 PL443827 A1 PL 443827A1 PL 443827 A PL443827 A PL 443827A PL 44382723 A PL44382723 A PL 44382723A PL 443827 A1 PL443827 A1 PL 443827A1
Authority
PL
Poland
Prior art keywords
stem cells
pluripotent stem
induced pluripotent
extracellular vesicles
derived
Prior art date
Application number
PL443827A
Other languages
Polish (pl)
Inventor
Sylwia BOBIS-WOZOWICZ
Milena PAW
Paweł FERDEK
Marcin PIEJKO
Dawid Wnuk
Agnieszka KUSIAK
Jacek LITEWKA
Kinga STOPA
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL443827A priority Critical patent/PL443827A1/en
Priority to PCT/PL2024/050013 priority patent/WO2024172676A1/en
Priority to EP24718302.3A priority patent/EP4665840A1/en
Publication of PL443827A1 publication Critical patent/PL443827A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/45—Artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Reproductive Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

W zgłoszeniu ujawniono lek do leczenia zwłóknień narządowych, który opracowany został na bazie pęcherzyków zewnątrzkomórkowych, pochodzących z indukowanych pluripotencjalnych komórek macierzystych hodowanych w warunkach fizjologicznej hipoksji.The application discloses a drug for the treatment of organ fibrosis, which was developed on the basis of extracellular vesicles derived from induced pluripotent stem cells cultured under physiological hypoxia conditions.

PL443827A 2023-02-18 2023-02-18 Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application PL443827A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PL443827A PL443827A1 (en) 2023-02-18 2023-02-18 Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application
PCT/PL2024/050013 WO2024172676A1 (en) 2023-02-18 2024-02-19 Method for obtaining extracellular vesicles (evs) derived from induced pluripotent stem cells (ipscs) and their use
EP24718302.3A EP4665840A1 (en) 2023-02-18 2024-02-19 Method for obtaining extracellular vesicles (evs) derived from induced pluripotent stem cells (ipscs) and their use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL443827A PL443827A1 (en) 2023-02-18 2023-02-18 Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application

Publications (1)

Publication Number Publication Date
PL443827A1 true PL443827A1 (en) 2024-08-19

Family

ID=90720481

Family Applications (1)

Application Number Title Priority Date Filing Date
PL443827A PL443827A1 (en) 2023-02-18 2023-02-18 Method of obtaining extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSC) and their application

Country Status (3)

Country Link
EP (1) EP4665840A1 (en)
PL (1) PL443827A1 (en)
WO (1) WO2024172676A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180273906A1 (en) * 2014-04-17 2018-09-27 Muhammad Ashraf Microvesicle and stem cell compositions for therapeutic applications

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANDRADE, A.C.; WOLF, M.; BINDER, H.-M.; GOMES, F.G.; MANSTEIN, F.; EBNER-PEKING, P.; POUPARDIN, R.; ZWEIGERDT, R.; SCHALLMOSER, K.: "Int. J. Mol. Sci. 2021, 22, 3890", HYPOXIC CONDITIONS PROMOTE THE ANGIOGENIC POTENTIAL OF HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED EXTRACELLULAR VESICLES, DOI: https://doi.org/10.3390/ijms22083890 *
BOBIS-WOZOWICZ S, MARBÁN E: "Front. Cell Dev. Biol., 2022, 10:919426", EDITORIAL: EXTRACELLULAR VESICLES AS NEXT GENERATION THERAPEUTICS.,, DOI: 10.3389/fcell.2022.919426 *
GUOKAI CHEN, DANIEL R. GULBRANSON, ZHONGGANG HOU, JENNIFER M. BOLIN, VICTOR RUOTTI, MITCHELL D. PROBASCO, KIMBERLY SMUGA-OTTO, SAR: "Nat Methods.; 2011, 8(5): 424–429", CHEMICALLY DEFINED CONDITIONS FOR HUMAN IPS CELL DERIVATION AND CULTURE, DOI: 10.1038/nmeth.1593 *
LOZANO, J.; RAI, A.; LEES, J.G.; FANG, H.; CLARIDGE, B.; LIM, S.Y.; GREENING, D.W.: "Int. J. Mol. Sci. 2022, 23, 14334", SCALABLE GENERATION OF NANOVESICLES FROM HUMAN-INDUCED PLURIPOTENT STEM CELLS FOR CARDIAC REPAIR, DOI: https://doi.org/10.3390/ijms232214334 *
MARTA ADAMIAK I IN.,: "Circ Res.; 2018, 122(2): 296–309", INDUCED PLURIPOTENT STEM CELL (IPSC)-DERIVED EXTRACELLULAR VESICLES ARE SAFER AND MORE EFFECTIVE FOR CARDIAC REPAIR THAN IPSCS, DOI: 10.1161/CIRCRESAHA.117.311769 *
NEA BISTER, CRISTIANA PISTONO, BENJAMIN HUREMAGIC, JUKKA JOLKKONEN, ROSALBA GIUGNO, TARJA MALM: "J Extracell Vesicles, 2020, 10:e12002", HYPOXIA AND EXTRACELLULAR VESICLES: A REVIEW ON METHODS, VESICULAR CARGO AND FUNCTIONS, DOI: https://doi.org/10.1002/jev2.12002 *

Also Published As

Publication number Publication date
WO2024172676A1 (en) 2024-08-22
EP4665840A1 (en) 2025-12-24

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