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PL2963709T3 - Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych - Google Patents

Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych

Info

Publication number
PL2963709T3
PL2963709T3 PL15179339T PL15179339T PL2963709T3 PL 2963709 T3 PL2963709 T3 PL 2963709T3 PL 15179339 T PL15179339 T PL 15179339T PL 15179339 T PL15179339 T PL 15179339T PL 2963709 T3 PL2963709 T3 PL 2963709T3
Authority
PL
Poland
Prior art keywords
methods
nucleic acids
transposon end
modifying nucleic
end compositions
Prior art date
Application number
PL15179339T
Other languages
English (en)
Inventor
Jerome Jendrisak
Gary Dahl
Haiying Li Grunenwald
Nicholas Caruccio
Original Assignee
Epicentre Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42119714&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2963709(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Epicentre Technologies Corporation filed Critical Epicentre Technologies Corporation
Publication of PL2963709T3 publication Critical patent/PL2963709T3/pl

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    • CCHEMISTRY; METALLURGY
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL15179339T 2008-10-24 2009-10-24 Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych PL2963709T3 (pl)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10832108P 2008-10-24 2008-10-24
US10832608P 2008-10-24 2008-10-24
US10832908P 2008-10-24 2008-10-24
US15286809P 2009-02-16 2009-02-16
US15543109P 2009-02-25 2009-02-25
US18453009P 2009-06-05 2009-06-05
EP15179339.5A EP2963709B1 (en) 2008-10-24 2009-10-24 Transposon end compositions and methods for modifying nucleic acids

Publications (1)

Publication Number Publication Date
PL2963709T3 true PL2963709T3 (pl) 2017-11-30

Family

ID=42119714

Family Applications (1)

Application Number Title Priority Date Filing Date
PL15179339T PL2963709T3 (pl) 2008-10-24 2009-10-24 Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych

Country Status (13)

Country Link
EP (6) EP2508529B1 (pl)
JP (1) JP5794572B2 (pl)
CN (2) CN102264914B (pl)
CA (1) CA2750054C (pl)
DK (4) DK2376517T3 (pl)
ES (5) ES2637843T3 (pl)
HK (1) HK1249765B (pl)
HU (1) HUE027961T2 (pl)
LT (1) LT2963709T (pl)
PL (1) PL2963709T3 (pl)
PT (2) PT2963709T (pl)
SI (1) SI2963709T1 (pl)
WO (1) WO2010048605A1 (pl)

Families Citing this family (190)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2240606B1 (en) 2008-01-14 2016-10-12 Applied Biosystems, LLC Compositions, methods, and kits for detecting ribonucleic acid
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3072968A1 (en) * 2010-02-25 2016-09-28 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
US20110224106A1 (en) 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CA2821299C (en) * 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9005935B2 (en) * 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
CN103732744A (zh) * 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
CA2844056A1 (en) * 2011-08-04 2013-02-07 Sage Science, Inc. Systems and methods for processing fluids
EP2783001B1 (en) * 2011-11-22 2018-01-03 Active Motif Multiplex isolation of protein-associated nucleic acids
US10689643B2 (en) * 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
NO2694769T3 (pl) 2012-03-06 2018-03-03
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
WO2013181265A1 (en) * 2012-05-29 2013-12-05 The Johns Hopkins University Eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
CN102703432A (zh) * 2012-07-11 2012-10-03 烟台博诺生物科技有限公司 构建核酸库的方法、试剂及试剂盒
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
CA2887341C (en) 2012-10-12 2021-03-16 Sage Science, Inc. Side-eluting molecular fractionator
WO2014071178A1 (en) * 2012-11-05 2014-05-08 Icahn School Of Medicine At Mount Sinai Method of isolating pure mitochondrial dna
CN103849672B (zh) * 2012-12-06 2017-06-06 中国科学院上海生命科学研究院 一组糖基转移酶及其应用
EP3561072A1 (en) * 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
BR112015019159A2 (pt) 2013-02-08 2017-07-18 10X Genomics Inc geração de código de barras de polinucleotídeos
GB201314695D0 (en) * 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
EP2970951B1 (en) * 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
SG10201708498VA (en) 2013-04-17 2017-11-29 Agency Science Tech & Res Method for generating extended sequence reads
EP2999784B2 (en) * 2013-05-22 2022-11-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
WO2014189957A2 (en) 2013-05-23 2014-11-27 The Board Of Trustees Of The Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9217167B2 (en) * 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
CN105705515B (zh) * 2013-11-07 2021-05-25 安捷伦科技有限公司 多种用于dna操作的转座酶适体
CN107002117B (zh) * 2013-12-05 2021-12-10 生捷科技控股公司 核酸测序方法
CN103710342A (zh) * 2013-12-18 2014-04-09 浙江农林大学 人工诱导的mle转座子试剂盒及其应用
AU2014364926B2 (en) 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
CN105899683A (zh) * 2014-01-14 2016-08-24 凯杰有限公司 带标签dna片段的产生
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
CN106103713B (zh) * 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US20170114333A1 (en) * 2014-04-11 2017-04-27 The Johns Hopkins University Improvements to eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
EP3845640A3 (en) 2014-04-15 2021-09-01 Illumina, Inc. Modified tranposases for improved insertion sequence bias and increased dna input tolerence
EP3680333A1 (en) 2014-04-29 2020-07-15 Illumina, Inc. Multiplexed single cell expression analysis using template switch and tagmentation
CA2949952C (en) 2014-05-23 2021-03-23 Fluidigm Corporation Haploidome determination by digitized transposons
CA3172086A1 (en) * 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
JP2017522866A (ja) 2014-06-26 2017-08-17 10エックス ジェノミクス, インコーポレイテッド 核酸配列の分析
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
ES2713153T3 (es) * 2014-06-30 2019-05-20 Illumina Inc Métodos y composiciones que utilizan la transposición unilateral
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
CN106715713B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 试剂盒及其在核酸测序中的用途
EP3192869B1 (en) * 2014-09-12 2019-03-27 MGI Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
CN105525357B (zh) * 2014-09-30 2018-08-21 深圳华大基因股份有限公司 一种测序文库的构建方法及试剂盒和应用
WO2016061098A1 (en) * 2014-10-14 2016-04-21 Covaris, Inc. Acoustic energy mediation of genetic fragmentation
EP3208345B1 (en) * 2014-10-14 2019-12-04 MGI Tech Co., Ltd. One-stop treatment method for breaking nucleic acid by means of transposase, and reagent
GB201418159D0 (en) * 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
DK3208335T3 (da) * 2014-10-14 2020-08-31 Mgi Tech Co Ltd Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens
CA2964487C (en) 2014-10-15 2024-03-19 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
PT3207134T (pt) * 2014-10-17 2019-09-17 Illumina Cambridge Ltd Transposição que preserva a contiguidade
KR102472027B1 (ko) * 2014-10-17 2022-11-30 일루미나 케임브리지 리미티드 근접 보존 전위
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP4239067A3 (en) 2014-11-05 2023-10-25 Illumina, Inc. Transposase compositions for reduction of insertion bias
ES2706531T3 (es) 2014-11-11 2019-03-29 Illumina Inc Amplificación de polinucleótidos empleando sistemas CRISPR-Cas
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN105985945B (zh) * 2015-01-30 2019-04-23 深圳华大智造科技有限公司 mRNA片段化方法及基于其构建测序文库的方法
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016169431A1 (zh) * 2015-04-20 2016-10-27 深圳华大基因研究院 一种长片段dna文库构建方法
EP3303621B1 (en) 2015-05-28 2025-07-02 Illumina Cambridge Limited Surface-based tagmentation
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
US10280449B2 (en) 2015-06-29 2019-05-07 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
CN108474029B (zh) 2015-08-07 2021-07-23 麻省理工学院 通过扩展显微法的蛋白质和核酸的纳米级成像
CA3172078A1 (en) 2015-08-28 2017-03-09 Illumina, Inc. Nucleic acid sequence analysis from single cells
EP3414255B1 (en) 2015-11-20 2021-09-22 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
CN115369161A (zh) 2015-12-04 2022-11-22 10X 基因组学有限公司 用于核酸分析的方法和组合物
CN105524920B (zh) * 2016-01-21 2018-10-02 深圳华大基因研究院 用于Ion Proton测序平台的TN5建库的引物组、试剂盒和建库方法
DK3455372T3 (da) 2016-05-11 2020-01-06 Illumina Inc Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
US10240196B2 (en) * 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2018042776A1 (ja) * 2016-08-30 2018-03-08 国立大学法人九州大学 Dna結合タンパク質の結合領域の近傍に所望のdna断片を挿入する方法
CN110139932B (zh) * 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN112986589B (zh) * 2017-01-05 2023-01-24 深圳迈瑞生物医疗电子股份有限公司 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10920219B2 (en) 2017-02-21 2021-02-16 Illumina, Inc. Tagmentation using immobilized transposomes with linkers
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
JP2020515250A (ja) 2017-04-07 2020-05-28 セージ サイエンス, インコーポレイテッド 統合型電気泳動dna精製を使用する遺伝的構造変動を検出するためのシステムおよび方法
MX2019013993A (es) * 2017-05-23 2020-07-28 Harvard College Amplificación de etiquetado extremo múltiple de ácidos nucleicos.
CN110678547B (zh) * 2017-05-23 2023-10-31 生物辐射实验室股份有限公司 分子条码化
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4345172A3 (en) 2017-06-05 2024-07-03 Becton, Dickinson and Company Sample indexing for single cells
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
CN107586835B (zh) * 2017-10-19 2020-11-03 东南大学 一种基于单链接头的下一代测序文库的构建方法及其应用
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112004920B (zh) 2018-02-05 2024-08-02 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
US11807903B2 (en) * 2018-02-08 2023-11-07 Universal Sequencing Technology Corporation Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN110219054B (zh) * 2018-03-04 2020-10-02 清华大学 一种核酸测序文库及其构建方法
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US12338486B2 (en) 2018-04-19 2025-06-24 Massachusetts Institute Of Technology Single-stranded break detection in double-stranded DNA
AU2019262048B2 (en) 2018-05-03 2025-09-04 Becton, Dickinson And Company High throughput multiomics sample analysis
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN113249796B (zh) * 2018-06-20 2024-08-27 深圳海普洛斯医学检验实验室 对dna片段进行标记的单分子标签
DK3821011T5 (da) * 2018-07-11 2024-08-19 Cygnus Biosciences Beijing Co Ltd Transposom-aktiveret dna/rna-sekventering (ted rna-seq)
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
CN112359090A (zh) 2018-08-07 2021-02-12 深圳市真迈生物科技有限公司 一种核酸片段的连接方法、测序文库的构建方法及其应用
US12297490B2 (en) 2018-08-28 2025-05-13 Sophia Genetics S.A. Methods for asymmetric DNA library generation and optionally integrated duplex sequencing
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
BR112021006183A2 (pt) 2018-11-30 2021-06-29 Illumina, Inc. análise de múltiplos analitos com o uso de um único ensaio
EP3899028A1 (en) * 2018-12-17 2021-10-27 Illumina, Inc. Methods and means for preparing a library for sequencing
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
KR20210114918A (ko) 2019-01-11 2021-09-24 일루미나 케임브리지 리미티드 복합체 표면-결합 트랜스포좀 복합체
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924505B1 (en) 2019-02-12 2025-12-17 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
CA3130889A1 (en) 2019-02-22 2020-08-27 Massachusetts Institute Of Technology Iterative direct expansion microscopy
WO2020210802A1 (en) * 2019-04-11 2020-10-15 University Of Virginia Patent Foundation Tagmentation to open up circles of dna and detect extrachromosomal circles of dna for diagnosis
CN110257479A (zh) * 2019-06-25 2019-09-20 北京全式金生物技术有限公司 一种快速构建rna 3’端基因表达文库的方法
JP7603586B2 (ja) 2019-07-12 2024-12-20 イルミナ ケンブリッジ リミテッド 電気泳動を用いた核酸ライブラリの調製
EP4004231B1 (en) 2019-07-22 2025-11-12 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
US20220282233A1 (en) * 2019-08-22 2022-09-08 New England Biolabs, Inc. Cleavage of Single Stranded DNA Having a Modified Nucleotide
WO2021048444A1 (en) 2019-09-12 2021-03-18 Thermo Fisher Scientific Baltics Uab Recombinant transposon ends
CN110564705A (zh) * 2019-09-30 2019-12-13 苏州璞瑞卓越生物科技有限公司 一种用于转座酶片段化的试剂及其应用
JP7489455B2 (ja) 2019-10-25 2024-05-23 チャンピン ナショナル ラボラトリー 哺乳類dnaのメチル化の検出及び分析
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
US20210172012A1 (en) 2019-12-04 2021-06-10 Illumina, Inc. Preparation of dna sequencing libraries for detection of dna pathogens in plasma
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
EP4097228B1 (en) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4407030B1 (en) 2020-06-02 2025-12-17 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
WO2021252617A1 (en) * 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
CN115552035A (zh) * 2020-06-09 2022-12-30 因美纳公司 用于增加测序文库产量的方法
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116194589A (zh) 2020-07-31 2023-05-30 贝克顿迪金森公司 用于转座酶可及染色质的单细胞测定
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022143221A1 (zh) * 2020-12-31 2022-07-07 中国科学院北京基因组研究所(国家生物信息中心) 用于标记核酸分子的方法和试剂盒
EP4294933A4 (en) * 2021-02-19 2025-10-15 Nuclear Rna Networks Inc COMPOSITIONS AND METHODS FOR MODULATING GENE TRANSCRIPTION NETWORKS BASED ON SHARED HIGH-IDENTITY TRANSPOSABLE ELEMENT SEQUENCES AND NON-PROCESSIVE PROMOTER TRANSCRIPTS AND PROMOTER-PROXIMAL TRANSCRIPTS
JP2024511766A (ja) 2021-03-29 2024-03-15 イルミナ インコーポレイテッド 改善されたライブラリ調製方法
EP4384634B1 (en) 2021-08-11 2025-05-21 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
EP4493721A1 (en) 2022-03-15 2025-01-22 Illumina, Inc. Parallel sample and index sequencing
EP4590854A1 (en) 2022-09-19 2025-07-30 Illumina, Inc. Deformable polymers comprising immobilised primers
CN115386622B (zh) * 2022-10-26 2023-10-27 北京寻因生物科技有限公司 一种转录组文库的建库方法及其应用
WO2024256580A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025083338A1 (en) * 2023-10-20 2025-04-24 Domus Biotechnologies Oy An in vitro method for fragmenting polynucleotides for next generation sequencing
CN118957058A (zh) 2023-10-20 2024-11-15 北京昌平实验室 用于可靠的无创胚胎植入前基因检测的方法
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
CA2167838C (en) 1993-07-23 1999-11-23 Thomas B. Ryder Methods for enhancing nucleic acid amplification
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
JP2000505312A (ja) 1996-03-18 2000-05-09 モレキュラー バイオロジー リソーシーズ,インコーポレイテッド 標的核酸配列増幅
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
FI103809B1 (fi) * 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
EA004327B1 (ru) 1999-03-19 2004-04-29 Такара Био. Инк. Способ амплификации последовательности нуклеиновой кислоты
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
IL148091A0 (en) 1999-09-13 2002-09-12 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
EA007414B1 (ru) 2000-08-23 2006-10-27 Такара Био Инк. Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности
EP1339863A2 (en) * 2000-12-05 2003-09-03 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
FR2822164B1 (fr) 2001-03-19 2004-06-18 Centre Nat Rech Scient Polypeptides derives des arn polymerases, et leurs utilisations
FI20020746A7 (fi) * 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
US7108976B2 (en) * 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
ATE391173T1 (de) 2002-09-20 2008-04-15 Prokaria Ehf Thermostabile ligase aus thermus phage
WO2004039953A2 (en) * 2002-10-28 2004-05-13 Xeotron Corporation Array oligomer synthesis and use.
ATE411400T1 (de) 2002-11-21 2008-10-15 Epict Technologies Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
JP4789271B2 (ja) * 2005-12-02 2011-10-12 シンセティック ゲノミクス、インク. エラーが最小化された核酸分子の合成
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays

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