PL246301B1 - Liquid disinfectant preparation and method of producing it - Google Patents

Description

Description of the invention

The subject of the invention is a liquid disinfectant preparation for disinfecting the body, surfaces of objects, also in contact with food, showing high antibacterial, antiviral, antifungal and antiyeast activity, with prolonged action.

Under normal circumstances, maintaining general hygiene and washing the body, hands and surfaces of everyday objects provides sufficient protection against the adverse effects of contact with microorganisms and infected objects.

However, there are situations in which such actions prove insufficient, as for example in the case of an epidemic of infectious diseases, especially those transmitted by droplets, when, in addition to ordinary hygiene procedures, it is advisable to function in conditions close to aseptic conditions. It is then recommended to frequently disinfect both hands and surfaces of everyday objects, with biocidal agents ensuring their microbiological purity.

In specific spaces of human activity, such as operating rooms and medical equipment and space for people with reduced immunity, it is also necessary to actively disinfect the air, equipment, instruments and apparatus, as well as maintain the sterility of the clothing of staff staying in these rooms, frequent hand disinfection and the use of appropriate safeguards to limit the spread of microorganisms via droplets.

There are various methods of disinfecting the surfaces of objects for specialized use and methods of disinfecting hands. Already in the mid-19th century, many years before Louis Pasteur discovered the pathogenicity of bacteria (1859), the Hungarian physician Ignac Philipp Semmelweis, working in a Vienna maternity clinic, discovered that washing hands and surgical instruments with calcium hypochlorite significantly reduced the spread of puerperal fever and mortality among patients (1847). The procedure of washing hands and surgical instruments with special antiseptic preparations that he proposed became routine in hospitals only in the second half of the 19th century, after the British surgeon Joseph Lister introduced the procedure of disinfection using a phenol solution or its aerosol (1867). Despite this, already during the Civil War (1861-1865), surgical operations were preceded by washing hands and disinfecting surgical instruments by immersing them in boiling water. A major contribution to the development of antiseptics was also made by the Polish surgeon Jan Mikulicz-Radecki, who used iodoform for disinfection and used disinfected cotton gloves and a mask covering the mouth and nose during operations (1880).

There are now many classical antiseptics used in everyday life and many specialized liquid and solid antiseptic preparations for specialized applications, many of which use ethanol, propanol or hydrogen peroxide as the active ingredient.

A method of chemical disinfection is known (PL 235547 B1), according to which the surface to be disinfected is treated for 0.5 to 2 hours with saponin obtained from Quillaja saponaria, then it is rinsed with water and then treated with a chemical disinfectant from the group consisting of quaternary ammonium salts (QAC), peracetic acid (PAA) and their derivatives, wherein pure saponin or in the form of an extract is used in concentrations of 0.1-5% w/v and at a temperature of 5 to 30°C.

A disinfecting composition for hygienic hand disinfection and hand washing is known (EP 1683418 B2). This composition comprises at least glycerol 1-(2-ethylhexyl) ether, octenidine dihydrochloride and cocoamidopropylamine oxide. Preferably, the composition comprises: 0.5-1% by weight of glycerol 1-(2-ethylhexyl) ether, 0.2-0.5% by weight of octenidine dihydrochloride, 8-12% by weight of cocoamidopropylamine oxide and 80-85% by weight of water, and also, if appropriate: 0.8-1.5% by weight of glycerol, 2-3% by weight of glycerol cocoate, 0.1-0.3% by weight of allantoin, 0.5-2% by weight of hydroxyethylcellulose and 0.6-1 wt.% lactic acid.

A mild and highly skin-compatible composition with a biocidal action for disinfection, sanitization, microbial regulation and sebum regulation is known (EP 2101720 B2). This composition is suitable for skin disinfection and comprises hydrogen peroxide in a concentration of 0.1-10% (w/w), preferably 0.2-6%, and an N-acylated amino acid and/or peptide, or a salt thereof, in the range of 0.1-20% (w/w), preferably 0.1-10%, more preferably 0.2-8% and most preferably 0.2-5%, wherein when the composition comprises an oil phase and an aqueous phase, the composition does not comprise a self-emulsifying base of Protelan ENS in a concentration of 20% (w/w). Preferably, this composition is an aqueous solution.

A hand disinfectant is known (EP 3133918 B2) comprising at least 40% by weight of water, at least one solvent ether, such as dipropylene glycol, tripropylene glycol or tetrapropylene glycol, at least one fatty substance in the form of phenoxyethanol, phenoxypropanol, isodecanol, isododecanol or isotridecanol, the content of said fatty substance being greater than its solubility in water, and 0.1-20% by weight of a microbicidal cationic surfactant, which is didecyldimethylammonium chloride, bromide or iodide, said agent containing neither ethanol nor propanol nor another alcohol having fewer than 9 carbon atoms.

A hand disinfectant and a method of producing and using the same are known (EP 3463269 B2), comprising hypochlorous acid (HOCl), acetic acid (HAc), water and an auxiliary substance, which is a thickening agent, such as, for example, inorganic thickeners, such as colloidal silica, synthetic clay materials or the product sold under the trade name LAPONITE from BYK Additives (Cheshire, UK), and optionally a chelating agent - such as, additionally or alternatively, EDTA, polyethylene glycol, polysorbate, glycerol, an acrylic copolymer, essential oils, buffers, cellulose derivatives or xanthan gum.

Aqueous disinfectants are known (DE 2701133 B2) produced from compounds that split off hydrogen peroxide and from aromatic acyloxycarboxylic acids. However, only a few of these compounds provide disinfectant solutions with a sufficiently broad spectrum of action, and the storage of these acylating agents in a mixture with the necessary inorganic peroxides is only possible for a limited time due to the decomposition reaction.

A solid disinfectant in the form of a powder comprising a peroxide, an acylating agent and a non-ionic surfactant and its use for disinfecting surfaces and instruments or devices, especially in the medical field, for destroying Gram-positive bacteria, mycobacteria and viruses is known. This agent, in the form of a powder based on active acidic substances, comprises a system producing peracetic acid from a peroxide and an N-acylating agent and non-ionic surfactants free of alkoxylated alkylphenols, comprising straight-chain ether alcohols or those containing a methyl branch in position 2, wherein the peroxide is preferably selected from the group of sodium perborate monohydrate, sodium perborate tetrahydrate, sodium percarbonate and mixtures thereof, and the N-acylating agent is preferably selected from the group of tetraacetylglycoluryl, tetraacetylethylenediamine, diacetylhexahydrotriazinedione and mixtures thereof.

A granular cleaning and disinfecting composition is known (EP 2326704 B2) which, when dissolved in water, produces a use solution containing a peroxyacid, comprising: 15-60 wt.% of percarbonate, 8-35 wt.% of an acylating agent, 0.5-5 wt.% of a non-ionic surfactant, 0.1-3 wt.% of a phosphonate as a foam inhibitor, with a bulk density in the range of 0.5-1.4 kg/l, characterized in that it comprises a mixture of at least two granulates, wherein one granulate comprises percarbonate and the other granulate comprises acylating agent, wherein the granulate comprising percarbonate is coated with a water-soluble inorganic salt and the granulate comprising the acylating agent is coated with a water-soluble surfactant.

There is a commercially available liquid disinfection and fogging agent known as Red'Ox Ag12, containing deionized water (88%), hydrogen peroxide (12%), colloidal silver (20 ppm) and orthophosphoric acid (440 ppm), with a pH of 3.8-5.0. The active substance, hydrogen peroxide, destroys microorganisms, while the colloidal silver layer additionally protects against re-colonization by microorganisms. Nevertheless, the disinfection procedure should be repeated several times a day, depending on the needs. The agent is used to wash surfaces, most often diluted with clean water in a ratio of 1: 1, but in the case of heavily soiled and porous surfaces, it is recommended to use it in undiluted form. It is required to apply the agent to the surface and then wipe it dry. The agent can be added to typical surfactant solutions. During fogging, the cleaned surface should be sprayed. The preparation can be used for disinfection and cleaning of horizontal and vertical surfaces, as well as for local disinfection, e.g. door handles, wipers, tools. The preparation can be used for fumigation. Unfortunately, the Red'Ox preparation is dangerous because it is harmful if swallowed and causes serious eye damage. It is recommended to use protective gloves, protective clothing and eye protection during use. In the event of dangerous situations, it is recommended to seek medical advice or seek medical care.

There is a commercially available liquid cleaning and disinfecting product known as PEROXOL, containing hydrogen peroxide (35%), with a pH of 5.1. The preparation is easily biodegradable and does not bioaccumulate. It does not foam and can be used at both low and high temperatures. It has bactericidal properties against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus hirae, Salmonella typhimurium and fungicidal properties, including Candida albicans and Aspergillus Niger. The preparation can be used for circulation, spray, immersion disinfection of surfaces in contact with food in the food industry, including tanks, pipelines, containers, devices and tools. It can also be used for disinfecting water rinsing products in the meat, poultry and vegetable industry, as well as an ingredient enhancing the cleaning properties of washing solutions based on sodium hydroxide. After disinfection, rinse the surface thoroughly with water. The preparation should be used in well-ventilated rooms. Due to the high concentration of hydrogen peroxide, the preparation is harmful if swallowed, irritates the skin and mucous membranes, causes serious eye damage, may cause respiratory tract irritation. Ingestion may lead to serious burns of the mouth and throat and perforation of the esophagus and stomach.

There is a well-known hydrogen peroxide disinfection fogger called Biosanitizer HY, which uses a 7% hydrogen peroxide solution. Dry fog fills the room, penetrates the nooks and crannies and disinfects them, including the walls, ceiling and other devices in the room, demonstrating a biocidal effect. It eliminates viruses, bacteria, fungi and spores from surfaces and rooms.

During the Covid-19 pandemic, wearing masks and protective gloves and frequent hand disinfection are mandatory, as well as disinfection of the surfaces of equipment and devices in public places such as shops, offices, public transport, to which numerous and random users have access.

Currently, the most commonly used liquid and aerosol hand and surface disinfectants use ethanol as an active antiseptic. Although the antiseptic effect of alcohol is effective and well-known, when used frequently, alcohol has a negative effect on the skin, causing dryness and degradation of the epidermis. Moreover, the action of alcohol preparations only allows for temporary disinfection, and the sterile surface very quickly becomes covered with pathogenic microorganisms again. In turn, the use of specialist agents on a large scale is impossible due to their high price and relatively low availability of ingredients.

There is therefore an unmet need for a cheap and easily available aseptic agent that is highly effective while being neutral towards the skin and that provides a more durable disinfection effect than known agents based on volatile alcohols that are clearly damaging to the epidermis of hands.

The essence of the invention

A liquid disinfectant preparation with antibacterial, antiviral, including anti-enveloped viruses, antifungal and antiyeast activity, containing hydrogen peroxide as an active substance, deionized water and phosphate ions, exhibiting an acidic reaction, characterized in that it contains:

3-5% by weight of hydrogen peroxide,

0.01-2% wt. glycerin,

0.01-0.06% by weight of a stabilizing and buffering mixture with the following composition:

0.0001-0.01 wt.% H3PO4,

0.005-0.02% by weight Na4.P2O10H2O,

0.005-0.02 wt.% Na2H2P2O7,

0.005-0.02 wt.% NaNO3,

0.005-0.02 wt.% Na2Sn(OH)6,

0.005-0.02 wt.% Na2SiO3, and 93-95 wt.% demineralized water, and has a pH in the range of 3-5, preferably 3-4.

According to the invention, the stabilizing and buffering mixture preferably contains: 0.001-0.01 wt.% H3PO4, 0.01 wt.% Na4P2O7^10H2O, 0.01 wt.% Na2H2P2O7, 0.01 wt.% NaNO3, 0.01 wt.% Na2Sn(OH)6, 0.01 wt.% Na2SiO3.

According to the invention, the disinfecting preparation additionally contains 0.005-0.02 wt.% of a detergent, preferably in the form of a mixture of anionic or nonionic surface-active compounds selected from sodium lauryl sulfate or ethoxylated alcohols with chain lengths in the range of C6-C15, preferably in the range of C10-C15, for example isotridecanol.

According to the invention, the preparation contains 4.9% by weight of hydrogen peroxide.

According to the invention, the preparation in the version for surface disinfection contains 0.01-0.5% by weight of glycerin, preferably 0.1% by weight of glycerin.

According to the invention, the preparation in the hand disinfection version contains 0.5-2% by weight of glycerin, preferably 1% by weight of glycerin.

The method of producing the liquid disinfectant preparation defined above, according to the invention, is characterized in that it consists of two stages, where:

In the first stage, a concentrate is produced by preliminary preparation of three solutions:

- 50-200 g of Na2Sn(OH)6 powder and 1-3 ml of 5% H3PO4 are added to 900 g of demineralized water, and the tank is left for 24 hours until a white colloidal suspension appears,

- 50-200 g of 30% Na2SiO3 solution and 1-3 ml of 5% H3PO4 are added to 900 g of demineralized water, and the tank is left for 24 hours until a white colloidal suspension appears,

- 1.33-266 g of 75% H3PO4 solution, 50-200 g of NaNO3 powder, 50-200 g of Na4P.-O10H?O powder and 50-200 g of Na2H2P2O7 powder are added to 2000 g of demineralized water and mixed until the ingredients are dissolved, then 100 g of detergent is optionally added, then 450 g of demineralized water is added and the whole is mixed gently, after which these three solutions are combined to form a concentrate weighing 5 kg and with a pH in the range of 0.7-2.7, and in the second stage, 5 kg of the obtained concentrate is mixed with 1-20 kg of glycerin, 85.7-142.9 kg of 35% aqueous hydrogen peroxide solution and diluted with deionized water to obtain a preparation weighing 1000 kg, with a pH in the range of 3-5, preferably 3-4.

According to the invention, in the first step, a detergent is used to prepare the concentrate, preferably in the form of a mixture of anionic or nonionic surface-active compounds selected from sodium lauryl sulphate or ethoxylated alcohols with chain lengths in the range of C6-C15, preferably in the range of C10-C15, for example isotridecanol.

According to the invention, in the first stage, 100 g of Na2Sn(OH)6 powder, 100 g of 30% Na2SiO3 solution, 13.3-133 g of 75% H3PO4 solution, 100 g of NaNO3 powder, 100 g of Na4P?O10H.-O powder, 100 g of Na2H2P2O7 powder and 100 g of detergent are used to produce the concentrate.

According to the invention, during dilution of the concentrate, 140 kg of a 35% aqueous solution of hydrogen peroxide is added and a preparation with a 4.9% H2O2 concentration is obtained.

According to the invention, in the version of the disinfectant preparation for surface disinfection, 1 kg of glycerin is added when diluting the concentrate.

According to the invention, in the version of the disinfectant preparation for hand disinfection, 10 kg of glycerin is added when diluting the concentrate.

According to the invention, during the preparation and storage of the concentrate and the preparation, containers, mixers, tools and pouring lines made exclusively of plastics are used.

Detailed description of the invention

The disinfectant preparation according to the invention has high disinfection efficiency, confirmed by appropriate laboratory tests (including PZH certificates), the results of which are presented in the examples. Laboratory tests have confirmed antibacterial, antiviral, including against enveloped viruses, antifungal and antiyeast activity. What is extremely important, the preparation according to the invention is safe for people and animals, as well as safe for cleaned surfaces, without destroying or causing corrosion.

The advantage of the disinfectant preparation, according to the invention, over currently used disinfectants, apart from the extended action time, is the lack of harmful impact on the environment. This agent is safe for people, does not destroy devices and equipment of disinfected objects, does not cause corrosion of metals and non-metals. It is also non-flammable, because it does not contain alcohol, which is very important for Muslims.

The main advantages of the disinfectant preparation according to the invention include a set of the following features:

- safe for the health of both humans and animals

- intended for disinfection of surfaces with and without contact with food

- proven effectiveness in the fight against COVID 19

- prolonged disinfecting effect

- leaves no marks or discoloration

- wide range of applications and high efficiency

- easy and safe application process

- safe transport and storage

- long shelf life, 24 months in accordance with the standards

- ease of production and availability of cheap ingredients in large quantities

- reduction of factors causing seasonal diseases: bacteria, viruses, allergenic pollen - no waste and harmfulness in the production process.

Composition of the disinfectant preparation

The active substance is hydrogen peroxide, with well-known disinfecting and biocidal properties. Hydrogen peroxide in diluted aqueous solutions (3%) is currently used for wound disinfection, which means that in low concentrations H2O2 is a safe substance for humans, especially for even damaged skin. In turn, solutions with higher concentrations (for example 8%, 12%, 35%) are used for specialist surface disinfection, but their use requires the use of personal protective equipment due to the too strong oxidizing properties of hydrogen peroxide. Eye protection is particularly important here, which is severely irritated in contact with H2O2 solutions with a concentration higher than 5% (EU Assessment Report regarding Hydrogen peroxide, March 2015, Finland). Hydrogen peroxide solutions with higher concentrations are possible to obtain, but are not used for surface disinfection due to low stability, high price and too strong oxidizing properties.

The disinfectant preparation, according to the invention, contains hydrogen peroxide in a concentration range of 3-5% by weight in an aqueous solution. This is a range that allows for effective disinfection of skin and usable surfaces (stronger effect than in the case of hydrogen peroxide) while ensuring safety for the eyes of users in the case of routine fogging of spaces and in the case of inadvertent introduction of this preparation into the eye.

The hydrogen peroxide content in the preparation according to the invention may vary within the range of 3-5% by weight and is preferably 4.9% by weight, which corresponds to the addition of 85.7-142.9 kg of a 35% aqueous hydrogen peroxide solution, preferably 140 kg of a 35% aqueous H2O2 solution, to produce 1000 kg of the preparation when diluting 5 kg of the concentrate.

The disinfectant also contains a stabilizing and buffering mixture, the components of which ensure the stability of hydrogen peroxide in an aqueous solution, inhibiting its decomposition into oxygen and water. The stabilizing and buffering mixture contains H3PO4, Na/.P.-O IOHO, Na2H2P2O7, NaNO3, Na2Sn(OH)6 and Na2SiO3. The stabilization of the hydrogen peroxide solution is ensured in two ways. Firstly, the optimal pH of the preparation is ensured, maximizing the stability of H2O2 molecules while being safe for the skin. Secondly, the absence of iron, manganese and heavy metal ions in the preparation is ensured by using demineralized water and adding complexing substances.

The addition of H3PO4 is intended to give the concentrate and the preparation a suitably acidic pH in the range of 3-5, preferably 3-4, which is known as the range of increased stability of hydrogen peroxide in aqueous solutions. According to the invention, the content of phosphoric acid (V) may range from 0.0001 to 0.03% by weight, preferably 0.001 to 0.01% by weight, which corresponds to the addition of 1.33 to 133 g of a 75% aqueous solution of H3PO4, preferably 13.3 to 133 g, to produce 5 kg of concentrate. The concentrate has a pH in the range of 0.7 to 2.7, which directly translates into obtaining a preparation with a pH in the range of 3-5 when diluting 5 kg of concentrate with demineralized water to a mass of 1000 kg.

The presence of phosphate ions in the concentrate and preparation is also intended to stabilize hydrogen peroxide by complexing iron, manganese and other heavy metal ions, catalyzing the decomposition of hydrogen peroxide according to the mechanism described by Fenton and Haber-Weiss. Phosphate and polyphosphate anions complex iron, manganese and heavy metal ions, saturating their coordination spheres, preventing access of hydrogen peroxide molecules to these ions.

In order to increase the complexing effect of phosphate ions introduced into the concentrate and the preparation in the form of phosphoric acid, the stabilizing and buffering mixture contains an addition of phosphate salts, for example Na4P2O7^10H2O, Na2H2P2O7. However, it is possible to use other salts, including phosphate acid salts, or classical phosphate buffers in high concentrations, which will additionally stabilize the pH of the preparation. According to the invention, the content of Na.-'.P.-O10H'O can vary within the range of 0.005-0.02% by weight, preferably 0.01% by weight, and the content of Na2H2P2O7 can vary within the range of 0.005-0.02% by weight, preferably 0.01% by weight. This corresponds to the addition of 50-200 g, preferably 100 g, of each of these powder components to produce 5 kg of concentrate.

The addition of NaNO3 is intended to additionally increase the stability of hydrogen peroxide in the preparation. Nitrate anions in the presence of hydrogen peroxide transform into a radical form, which captures iron oxoforms, quenching the Fenton and Flaber-Weiss reactions. According to the invention, the NaNO3 content can vary in the range of 0.005-0.02% by weight, preferably 0.01% by weight, which corresponds to the addition of 50-200 g, preferably 100 g, of this powder component to produce 5 kg of concentrate.

The addition of Na2Sn(OH)6 and Na7SiO3 is intended to provide an additional method of stabilizing hydrogen peroxide in the preparation. Each of these compounds forms a colloidal system in acidic aqueous solutions that absorbs iron ions and heavy metals. According to the invention, the content of Na2Sn(OH)6 may vary within the range of 0.005-0.02% by weight, preferably 0.01% by weight, and the content of Na7SiO3 may vary within the range of 0.005-0.02% by weight, preferably 0.01% by weight. This corresponds to the addition of 50-200 g, preferably 100 g, of each of these powder components to produce 5 kg of concentrate.

In order to additionally enhance the effect of the components of the stabilizing and buffering mixture, according to the invention, demineralized, deionized or distilled water is used, preferably purified by reverse osmosis, with a preliminary distillation step to minimize the risk of introducing iron ions and heavy metal ions into the concentrate and preparation. In addition, all solutions are prepared and stored only using vessels, systems and tools made of plastics, because the use of metal can result in contamination of the preparation and thus lead to the decomposition of the active ingredient, i.e. hydrogen peroxide.

The stabilizing and buffering mixture, according to the invention, ensures innovative and effective stabilization of the preparation by minimizing the risk of exposure of hydrogen peroxide to iron, manganese and other heavy metal ions in three independent ways, i.e. by providing complexing components (phosphate anions), capturing components (nitrate radicals) and adsorbing components (a colloid made of Na2Sn(OH)6 and a colloid made of Na2SiO3).

The extended time of effective action of the disinfectant preparation, according to the invention, results from the use of components forming a thin film with an active agent, which has an antiseptic effect until it is washed off the surface or decomposes on its own. The active film, tightly covering the surface, does not allow viruses, bacteria and fungi to re-colonize it for up to 24 hours after application. The active film is created thanks to the use of non-aqueous additives (glycerin, surfactant), which do not allow the water fraction containing stabilized hydrogen peroxide to completely evaporate, thanks to which its activity is maintained for a long time. Otherwise, i.e. when the water fraction evaporates completely, the hydrogen peroxide would also evaporate, leaving the surface unprotected. A similar effect of leaving the surface unprotected is observed when using alcohol preparations, which after effective disinfection evaporate completely.

On the other hand, the use of non-aqueous additives in the preparation (glycerin, surfactant) reduces the surface tension of the water fraction, allowing for better surface wettability, better pore penetration and the creation of a dry mist composed of smaller drops. The addition of a surfactant is of particular importance here. Thanks to this, the contact effectiveness of the disinfectant preparation, according to the invention, significantly exceeds the effectiveness of classic water preparations, and even exceeds the effectiveness of alcohol preparations (due to the extended time of action), showing excellent wettability of surfaces and pores and all hard-to-reach spaces.

The addition of detergent, i.e. surfactant, is particularly important for reducing the surface tension of the preparation, since the addition of glycerin alone has a limited effect in this respect. As detergent, preferably anionic or nonionic surfactants or mixtures thereof are used. Preferably, sodium lauryl sulfate (SDS) or ethoxylated alcohols with chain lengths in the range of C6-C15, preferably ethoxylated fatty alcohols with chain lengths of C10-C15, are used. The use of isotridecanol (C13) is particularly preferred. According to the invention, the detergent content can vary in the range of 0.005-0.02% by weight, preferably 0.01% by weight, which corresponds to the addition of 50-200 g, preferably 100 g, of detergent to produce 5 kg of concentrate.

The glycerin content in the preparation according to the invention may vary in the range of 0.01-2% by weight, preferably 0.1-1% by weight, which corresponds to the addition of 0.1-20 kg, preferably 1-10 kg, of glycerin to produce 1000 kg of preparation when diluting 5 kg of concentrate.

The disinfectant preparation, according to the invention, is used both for disinfecting the body (mainly hands) and for disinfecting usable surfaces and room spaces.

The body disinfection preparation contains a larger addition of glycerin (0.5-2%, preferably 1%), which increases the comfort of use, giving the skin a soft feel to the touch. Glycerin, as a classic agent used in cosmetics, has a soothing and caring effect on the skin, comparable to the effect of glycerin hand creams. In addition, it increases the effectiveness of penetration of skin cavities and strengthens the process of creating an active film after evaporation of most of the water fraction. Then, the active film is able to stay on the skin of the hands longer. The preparation, known under the trade name AntiVir4 HAND, has obtained the permission of the Office for Registration of Medicinal Products, Medical Devices and Biocidal Products No. 8792/22 for making it available on the market and use in the territory of the Republic of Poland as a biocidal product.

The preparation in the variant for disinfection of usable surfaces contains a smaller addition of glycerin (0.01-0.5%, preferably 0.1%), which is intended to ensure the effective formation of an active film on disinfected surfaces and to reduce the surface tension of the preparation, increasing the wettability of the surface and penetration of pores and the creation of a dry mist with smaller drops during the fogging process. The preparation, known under the trade name AntiVir4 SURF, has obtained national permission from the Office for Registration of Medicinal Products, Medical Devices and Biocidal Products No. 8794/22 for making available on the market and use in the territory of the Republic of Poland as a biocidal product.

The composition of the preparation was selected and optimized with the aim of ensuring maximum biocidal activity of hydrogen peroxide, while ensuring its highest possible kinetic stability. The cost of the ingredients and their availability on the market in large quantities were also taken into account. The composition of the disinfectant preparation, according to the invention, provides a stable, high-class disinfectant product with well-documented antiviral, antibacterial, antifungal and antiyeast activity with prolonged action, possible to produce by simple industrial methods in large quantities using cheap and available domestic raw materials.

Method of obtaining a disinfectant preparation

The disinfectant preparation according to the invention is obtained in a procedure using only tools, systems and vessels made of plastics in order to prevent contamination of the preparation with ions of iron, manganese and other heavy metals.

The method of producing a liquid disinfectant preparation consists of two stages, of which the first stage concerns the production of a concentrate, and the second stage concerns the production of the actual preparation, in the appropriate variety. What is extremely important, the concentrate does not contain hydrogen peroxide and glycerin, in order to simplify the procedure of preparation of the preparation and to prevent premature deactivation of the active substance.

The concentrate is a concentrated solution containing a stabilizing and buffering mixture of appropriate concentrations and pH, with its minimal volume, which ensures ease of transport and handling of the ingredients during the subsequent stage of preparation of the preparation.

The concentrate is produced by preliminary preparation of three component solutions, two of which contain the components of the colloidal suspension and the third contains the remaining solid and liquid components.

The colloidal suspension formed from Na2Sn(OH)6 is prepared by adding 50-200 g of Na2Sn(OH)6 powder and 1-3 ml of 5% H3PO4 to 900 g of demineralized water to give the appropriate pH of this solution. Then, the tank is left for 24 h until a white, homogeneous colloid appears.

The Na2SiO3 colloidal solution is prepared by adding 50-200 g of 30% water glass to 900 g of demineralized water and 1-3 ml of 5% H3PO4 to give the appropriate pH to this solution. Then, the container is set aside for 24 h until a white, homogeneous colloid appears.

The concentrate solution is prepared by adding 1.33-133 g of 75% H3PO4 solution, 50-200 g of NaNO3 powder, 50-200 g of N84P2O7-10H2O powder and 50-200 g of Na2H2P2O7 powder to 2000 g of demineralized water, and then mixing everything until the ingredients dissolve. Then, a mixture of 50-200 g of detergents and 450 g of demineralized water is added to the resulting solution, and then mixing everything gently, preventing excessive foaming of the solution. Preferably, 100 g of each of the above-mentioned ingredients is added, which significantly simplifies the concentrate preparation procedure. It should be noted that increasing the content of ingredients will increase the stability of the later preparation and its ability to wetting surfaces, while increasing production costs. The previously obtained colloidal solutions are combined with the actual solution to create a 5 kg concentrate with a pH in the range of 0.7-2.7.

The pH of the concentrate is directly related to the amount of phosphoric acid added. For example, adding a small amount of H3PO4 (e.g. 1.33 g) will result in a concentrate with a pH of 2.7, while adding a moderate amount of H3PO4 (e.g. 13.3 g) will result in a concentrate with a pH of 1.7, and adding a large amount of H3PO4 (e.g. 133 g) will result in a concentrate with a pH of 0.7. Intermediate pH values are easily obtained by using the appropriate amount of phosphoric acid.

In the second stage of preparation of the preparation, 5 kg of the obtained concentrate is mixed with 1-20 kg of glycerin, 85.7-142.9 kg of 35% aqueous hydrogen peroxide solution, and diluted with deionized water to obtain a preparation weighing 1000 kg, having a pH in the range of 3-5, preferably 3-4.

The glycerin content is selected based on the application of the preparation. The preparation for hand disinfection requires the addition of 5-20 kg of glycerin, preferably 10 kg. In turn, the preparation for disinfection of usable surfaces requires the addition of 0.1-5 kg of glycerin, preferably 1 kg.

The amount of hydrogen peroxide solution added is selected based on the strength of the preparation. Using a smaller amount of H2O2 solution allows for lower production costs, but it is recommended to prepare a preparation with a concentration of hydrogen peroxide as close to 5% as possible, most preferably 4.9%. Then, the disinfecting capacity of the preparation is maximized, but it is safely avoided to exceed the limit value of 5%, above which hydrogen peroxide could cause eye irritation.

The pH of the preparation depends directly on the pH of the concentrate. Using a concentrate with a pH of 2.7 will allow you to obtain a preparation with a pH of 5, while using a concentrate with a pH of 1.7 will allow you to obtain a preparation with a pH of 4, and using a concentrate with a pH of 0.7 will allow you to obtain a preparation with a pH of 3. Intermediate pH values are easy to obtain using a concentrate with the appropriate pH.

According to the invention, however, it is possible to prepare a disinfectant preparation in any way, as long as its composition, showing antiviral, antibacterial, antifungal and antiyeast activity, is retained.

Properties of the disinfectant

The disinfection preparation, according to the invention, has high, scientifically proven disinfection effectiveness against viruses, bacteria, fungi and yeasts. The preparation, available under the trade name AntiVir4, has been admitted to the Polish market based on the permits of the Office for Registration of Medicinal Products, Medical Devices and Biocidal Products No. 8792/22 (skin disinfection preparation) and No. 8794/22 (preparation for disinfection of utility surfaces and for veterinary applications).

The drying time of the disinfectant preparation, according to the invention, depends on the ambient temperature and is up to 5 hours. Usually, however, the preparation dries in less than 2-3 minutes. The preparation remains on the surface thanks to the formation of an active film, which extends its action time. After some time, however, the active substance (hydrogen peroxide) on the disinfected surface undergoes automatic degradation, leaving no harmful and toxic substances.

The effectiveness of the preparation is very high. In less than one minute from the application of the preparation, all viruses, bacteria, fungi and yeasts on the disinfected surface are killed. In addition, because the liquid disinfectant is created on the basis of water, the disinfected skin is not destroyed, which is often observed with long-term use of alcohol preparations.

The preparation in the body disinfection variant is used in a similar way to currently used disinfectants, i.e. the hands are wiped with an appropriately large portion of the preparation, which evaporates over time, leaving a thin active film on the hands, showing a prolonged antiseptic effect.

The preparation in the variant for disinfection of usable surfaces can be used classically, by applying the liquid to the surfaces, for example with a moistened material, or by using the fogging or atomization technique, which is particularly advantageous due to the speed of action and the possibility of reaching all surfaces in a given space simultaneously. Fogging is carried out using classical methods, for example ultrasonic foggers. The generated dry fog has disinfecting properties identical to the properties of the preparation used classically. Dry fog, settling on surfaces, creates an active film on them with a prolonged effect.

Disinfection by atomization is recommended for offices and disinfection in residential areas, including toilets. The preparation can be applied using an atomizer with a spray nozzle, which generates a light mist covering the disinfected areas, and then the surface is wiped with a cloth or paper towel. In turn, fogging is recommended for both small and large spaces. It is intended for surface disinfection and decontamination in all industries, in public buildings, warehouses, hospitals and others.

The disinfectant, the method of its preparation and its antiviral, antibacterial and antifungal effects are described below in the examples. All substances used in the examples were characterized by a purity above 95%. A hydrogen peroxide solution from Grupa Azoty (Puławy) was used. Demineralized water from Piotr Parys (Lublin) was used. Quick measurements of the degree of surface cleaning were tested with a KIKOMAN Lumitester SMART luminometer, whose readings below 400 are considered to confirm microbiological purity, and values below 10 indicate sterile conditions.

Example 1. A concentrate with pH = 0.7 was produced for a disinfectant with pH = 3. A colloidal suspension of Na2Sn(OH)6 was prepared by adding 100 g of Na2Sn(OH)6 powder and 3 ml of 5% H3PO4 to 900 g of demineralized water in a 1 L polypropylene tank. Then, the tank was set aside for 24 h until a white, homogeneous colloidal suspension appeared. A colloidal suspension of Na2SiO3 water glass was prepared by adding 100 g of 30% Na2SiO3 water glass and 3 ml of 5% H3PO4 to 900 g of demineralized water in a 1 L polypropylene tank. Then, the tank was set aside for 24 h until a white, homogeneous colloidal suspension appeared, in a 5 L container made of polypropylene, the actual solution of the concentrate was prepared by adding 133 g of 75% H3PO4 solution, 100 g of NaNO3 powder, 100 g of Na4P.-O10H'O powder and 100 g of Na2H2P2O7 powder to 2000 g of demineralized water, after which the whole was stirred until the ingredients dissolved. Then, a mixture of 100 g of detergents and 450 g of demineralized water was added to the resulting solution, after which the whole was gently stirred, preventing excessive foaming of the solution. The previously obtained colloidal suspensions were combined with the actual solution to create a concentrate weighing 5 kg, showing a pH of 0.7.

Example 2. A concentrate with pH = 0.7 for a disinfectant preparation with pH = 3 was prepared as in Example 1, with the difference that instead of 100 g, 50 g of each solid ingredient was measured.

Example 3. A concentrate with pH = 0.7 for a disinfectant preparation with pH = 3 was prepared as in Example 1, with the difference that instead of 100 g, 200 g of each solid ingredient was measured.

Example 4. A concentrate with pH = 1.7 was prepared for a disinfectant preparation with pH = 4. The procedure was as in Example 1, with the difference that instead of 133 g of 75% H3PO4 solution, only 13.3 g of this ingredient was added.

Example 5. A concentrate with pH = 2.7 was prepared for a disinfectant preparation with pH = 5. The procedure was as in Example 1, with the difference that instead of 133 g of 75% H3PO4 solution, only 1.33 g of this ingredient was added.

Example 6. A disinfectant preparation was produced in a variant for hand disinfection, exhibiting pH = 3. For this purpose, a 5 kg concentrate, exhibiting pH = 0.7, produced in Example 1, was used. 5 kg of the concentrate and a portion of 100 L of demineralized water were added to a 1000 L polypropylene tank, and then 10 kg of glycerin, 140 kg of 35% aqueous hydrogen peroxide solution were added, after which the whole was diluted, the level of demineralized water was supplemented until the preparation weight reached 1000 kg. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 1% glycerin and exhibited pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. A disinfecting effect was observed and confirmed by lumimetric measurements, observing a reading oscillating around level 10.

Example 7. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with reduced H2O2 content. The procedure was as in Example 6, with the difference that 90 kg of a 35% aqueous solution of hydrogen peroxide was added. The obtained disinfectant preparation contained 3.1% hydrogen peroxide, 1% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 8. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with a moderate H2O2 content. The procedure was as in Example 6, with the difference that 115 kg of a 35% aqueous solution of hydrogen peroxide was added. The obtained disinfectant preparation contained 4.0% hydrogen peroxide, 1% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 9. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with increased glycerin content. The procedure was as in Example 6, with the difference that 20 kg of glycerin was added. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 2% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 10. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with reduced glycerin content. The procedure was as in Example 6, with the difference that 5 kg of glycerin was added. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 0.5% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 11. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 4. The procedure was as in Example 6, with the difference that the concentrate produced in Example 4 was used for its production. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 1% glycerin and showed pH = 4. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 12. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 5. The procedure was as in Example 6, with the difference that the concentrate produced in Example 5 was used for its production. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 1% glycerin and showed pH = 5. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 13. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with a reduced concentration of powder components. The procedure was as in Example 6, with the difference that the concentrate produced in Example 2 was used for its production. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 1% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 14. A disinfectant preparation was produced in a variant for hand disinfection, showing pH = 3 with an increased concentration of powder components. The procedure was as in Example 6, with the difference that the concentrate produced in Example 3 was used for its production. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 1% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfecting effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 15. A disinfectant preparation was produced in a variant for disinfecting usable surfaces, exhibiting pH = 3. For this purpose, a 5 kg concentrate, exhibiting pH = 0.7, produced in Example 1, was used. 5 kg of the concentrate and a portion of 100 L of demineralized water were added to a 1000 L polypropylene tank, and then 1 kg of glycerin, 140 kg of 35% aqueous hydrogen peroxide solution were added, after which the whole was diluted, the level of demineralized water was supplemented until the preparation weight reached 1000 kg. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 0.1% glycerin and exhibited pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. A disinfecting effect was observed and confirmed by lumimetric measurements, observing a reading oscillating around level 10.

Example 16. A disinfectant preparation was produced in a variant for disinfecting usable surfaces, showing pH = 3 with increased glycerin content. The procedure was as in Example 15, with the difference that 5 kg of glycerin was added. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 0.5% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfectant effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 17. A disinfectant preparation was produced in a variant for disinfecting usable surfaces, showing pH = 3 with reduced glycerin content. The procedure was as in Example 6, with the difference that 0.1 kg of glycerin was added. The obtained disinfectant preparation contained 4.9% hydrogen peroxide, 0.1% glycerin and showed pH = 3. The preparation was poured into 1 L containers made of polypropylene. The preparation was characterized by the stability of the active substance - no increase in pressure in the containers was observed. The disinfectant effect was observed, which was confirmed by lumimetric measurements, observing a reading oscillating around the level of 10.

Example 18. Independent laboratory tests were carried out on the biocidal effectiveness of the disinfectant preparation in the hand disinfection variant, obtained in Example 6, containing 4.9% hydrogen peroxide, 1% glycerin and showing pH = 3. The tests were performed in the environmental research laboratory of EKOLABOS Sp. z o.o. in Wrocław in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws of 2015 item 1926) and the relevant European standards regarding phase 2 / stage 2 tests: PN-EN 13697+A1:2019-08, PN-EN 1650:2019-12, PN-EN 14675+2015-06, PN-EN 14349:2013-05. Phase 2 stage 1 studies involve the use of a dilution and neutralization method, in which the test organism is exposed to the preparation at various concentrations, times and temperatures with the addition of burdening substances. These methods are intended to confirm the product's performance in laboratory conditions similar to the intended use.

RB/6240/10/21

The aim of the tests was to assess the biocidal efficacy of the product against the strains Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442, Escherichia coli ATCC 10536, Enterococcus hirae ATCC 10541, Salmonella typhimurium ATCC 14028, Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. The tests were performed in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015, item 1926) and in accordance with the European standard PN-EN 13697+A1:2019-08 Chemical disinfectants and antiseptics - Quantitative method for the evaluation of bactericidal and fungicidal effect of chemical disinfectants used in the food sector, industrial conditions and households and public facilities on non-porous surfaces. Test method and requirements (Phase 2, Step 2). According to the standard, a disinfectant has a biocidal effect on the strain used if the measured log reduction of bacterial cells is >4 and of yeast and mould cells >3.

The following conditions were used: Incubation 24 h at 37°C ±1°C for bacteria, 48 h at 30°C ±1°C for yeasts, 5 days at 30°C ±1°C for moulds. Number of test repetitions for a microorganism: 1. Test temperature: 20°C ±1°C. Mandatory contact time of the product with a bacterial suspension: 5 min ±10 s. Mandatory contact time of the product with a yeast or mould suspension: 15 min ±10 s. Incident substances: bovine albumin 3 g/l. Diluent used during the test: distilled water. Stability of the mixture of product with diluent: No precipitate formed during the test. Method used: neutralization of solutions. Counting method: deep plate plating. Neutralizer used, composition: Polysorbate 80 (30 g/l); Sodium thiosulfate (10 g/l); Lecithin (3 g/l). The neutralizer used allowed the validation of the method. Media used: Trypticasein Soy LAB-Agar (TSA) for bacteria and Malt-extract Agar (MEA) for yeasts and molds.

PL 246301 Β1

Bacteria Validation Test Results

Test organism Bacterial suspension for testing Neutralizer Toxicity Test Validation study Water test N NT NC Nc Staphylococcus aureus ATCC 6538 ΙΟ ⁶ : >330 10 ⁷ : 43 N: 7.03 NT:6.90 NC: 6.88 Nc: 6.91 Pseudomonas aeruginosa ATCC 15442 10' ⁵ : >330 ΙΟ ⁷ : 38 N: 6.98 NT: 6.96 NC: 6.93 Nc: 6.97 typhimurium ATCC 14028 10' ⁶ : >330 10' ⁷ : 45 N: 7.05 NT: 6.95 NC: 6.95 Nc: 6.96 Escherichia coli ATCC 10536 10' ⁶ : >330 10' ⁷ : 38 N: 6.98 NT: 6.86 NC: 6.89 Nc: 6.90 Enterococcus hirae ATCC 10541 10- ⁶ : >330 10' ⁷ : 41 N: 7.01 NT: 6.89 NC: 6.92 Nc: 6.94

N-logio of the number of cfu/ml applied to the test surface

NT - logio number of cfu/ml on the surface intended for the neutralizer toxicity test

NC - logio number of cfu/ml on the surface intended for validation testing

Nc - logio number of cfu/ml on the surface intended for testing in the control with water

Nts - number of cfu remaining on the surface after the test

Bacterial test results

Test organism Nc Results for individual concentrations in % by volume of the product (test conditions: contact time: 5 min, temperature: 20°C ± 1°C) 100% 1% 0.1% Staphylococcus aureus ATCC 6538 6.91 10-0:7110-1x14 10-2x14 10-0:>330 10-l:>330 10-2:>330 10-0:>330 10-l:>330 10-2:>330 Sun:2.85 Nts:0 Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nd) R: 4.06 R: <1.39 R: <1.39 Pseudomonas aeruginosa ATCC 15442 6.97 10-0x14 10-1X14 10-2Χ14 10-0:>330 10-l:>330 10-2:>330 10-0:>330 10-1:>330 10-2:>330 Sun:<2.15 Nts:0 Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nc) R: >4.83 R: <1.45 R: <1.45 Escherichia coli ATCC 10536 6.90 10-0x14 10-1x14 10-2x14 10-0:>330 10-l:>330 10-2:>330 10-0:>330 10-1:>330 10-2:>330 Sun:<2.15 Nts:0 Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nd) R: >4.76 R: <1.38 R: <1.38 Enterococcus hirae ATCC 10541 6.94 10-0x14 10-1x14 10-2x14 10-0:>330 10-l:>330 10 2:>33O 10-0:>330 10-l:>330 10-2:>330 Sun:<2.15 Nts:O Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nd) R: >4.79 R: <1.42 R: <1.42 Salmonella typhimurium ATCC 14028 6.96 10-0:<14 10-1X14 10-2x14 10-0:>330 10-l:>330 10-2:>330 10-0:>330 10-1:>330 10-2:>330 Sun:<2.15 Nts:0 Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nd) R: >4.81 R: <1.44 R: <1.44

Nd - logio number of cfu/ml on the surface intended for testing the effectiveness of the disinfectant

R-reduction of the number of microorganisms during the test

Nts - number of cfu remaining on the surface after the test

PL 246301 Β1

Yeast and mold validation test results

Test organism Bacterial suspension for testing Neutralizer Toxicity Test Validation study Water test N NT NC Nc brasiliensis ATCC 15404 10-5: >165 10-6: 30 N: 5.88 NT: 5.72 NC: 5.73 Nc: 5.76 Candida albicans ATCC 10231 10-5: >330 10-6: 41 N: 6.01 NT: 5.92 NC: 5.94 Nc: 5.95

N — logio of the number of cfu/ml deposited on the test surface

NT - logio number of cfu/ml on the surface intended for the neutralizer toxicity test

NC - logio of the number of cfu/ml on the surface intended for validation testing

Nc - logio number of cfu/ml on the surface intended for testing in the control with water

Nts - number of cfu remaining on the surface after the test

Bacterial test results

Test organism Nc Results for individual concentrations in % by volume of the product (test conditions: contact time: 5 min, temperature: 20°C+ 1°C) 100% 1% 0.1% Aspergillus brasiliensis ATCC 16404 5.76 10-0:<14 10-l:<14 1O-2:<14 10-0:>165 10-1: >165 10 2: >165 10-0:>165 10-1: >165 10-2: >165 Sun:<2.15 Nts:0 Sun:>5.22 Nts:>165 Sun:>5.22 Nts:>165 R=(Nc-Nd) R: >3.61 R:<0.54 R:<0.54 Candida albicans ATCC 10231 5.95 10-0:<14 10-l:<14 10-2:<14 10-0:>330 10-l:>330 10-2:>330 10-0:>330 10-l:>330 10-2:>330 Sun:<2.15 Nts:0 Nd:>5.52 Nts:>330 Nd:>5.52 Nts:>330 R=(Nc-Nc) R: >3.80 R: <0.43 R: <0.43

Nd - logio number of cfu/ml on the surface intended for testing the effectiveness of the disinfectant

R-reduction of the number of microorganisms during the test

Nts - number of cfu remaining on the surface after the test

The product tested according to the PN-EN 13697+A1:2019-08 standard, after a contact time of 5 min., temperature of 20°C, in the presence of a loading substance, shows a bactericidal effect on surfaces (reduction >4 log) against:

Staphylococcus aureus ATCC 6538 at 100% concentration Pseudomonas aeruginosa ATCC 15442 at 100% concentration Escherichia coli ATCC 10536 at 100% concentration Enterococcus hirae ATCC 10541 at 100% concentration Salmonella typhimurium ATCC 14028 at 100% concentration

The product tested according to the PN-EN 13697+A1:2019-08 standard, after a contact time of 15 min., temperature of 20°C, in the presence of a loading substance, shows fungicidal activity on surfaces (reduction >3 log) against:

Aspergillus brasiliensis ATCC 16404 at 100% concentration

Candida albicans ATCC 10231 at 100% concentration

RB/6241/10/21

The aim of the tests was to assess the effectiveness of the fungicidal or yeast-like fungicidal effect of the product against the strains Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. The tests were performed in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015, item 1926) and in accordance with the European standard PN-EN 1650:2019-12 Chemical disinfectants and antiseptics - Quantitative suspension method for the evaluation of fungicidal or yeast-like fungicidal effect of chemical disinfectants and antiseptics used in the food, industrial, household and institutional areas - Test method and requirements (phase 2, stage 1). According to the standard, the disinfectant has a biocidal effect against the strain used if the logarithm of the reduction of microbial cells obtained during the test is >4. Identification of yeast and mold strains: Aspergillus brasiliensis ATCC 16404 incubation 120 h at 30°C ±1 °C; Candida albicans ATCC 10231 incubation 48 h

PL 246301 Β1 at 30°C ±1°C. Number of test repetitions on the microorganism: 1. Test temperature: 20°C ±1°C. Contact time of the product with the microorganism suspension: 60 s ±10 s. Interfering substances: bovine albumin 3 g/l. Diluent used during the test: distilled water Stability of the product-diluent mixture: The product is stable during the test. Method used: neutralization of solutions. Counting method: deep-plate inoculation. Neutralizer used, composition: Polysorbate 80 (30 g/l) and sodium thiosulfate (10 g/l), Lecithin (3 g/l). The neutralizer used allowed the validation of the method. Medium used: Malt-extract Agar (MEA).

Yeast and mold validation test results

Test organism Bacterial test suspension Validation bacterial suspension Validation study Neutralizer Toxicity Control Water test N NvO And B C Aspergillus brasiliensis ATCC16404 N: 7.48 Nv0: 72 A: 70 B: 71 C: 65 Candida albicans ATCC 10231 N: 7.59 Nv0: 79 A: 74 B: 78 C: 73

N -log of the number of cfu/ml introduced into the test suspension

Nv0 -1/10 cfu/ml in validation suspension

A- number of cfu/ml in the mixture intended for control with water

B- number of cfu/ml in the mixture intended to control the toxicity of the neutralizer

C- number of cfu/ml in the mixture intended for the validation study

Bacterial test results

Test organism YEAH Results for individual concentrations in % by volume (test conditions: contact time: 60 s, temperature: 20°C ± 1°C) 80% 10% 1% Aspergillus brasiliensis ATCC 16404 6.48 <14, <14 >165, >165 >165, >165 Nx: <140 Na: <2.15 Nx: >1650 On: >3.22 Nx: >1650 On: >3.22 R (NO -Na) R: >4.33 R: <3.26 R: <3.26 Candida albicans ATCC 10231 6.59 <14, <14 >330, >330 >330, >330 Nx: <140 Na: <2.15 Nx: >3300 Na: >3.52 Nx: >3300 Na: >3.52 R (NO-Na) R: >4.44 R: <3.07 R: <3.07

NO-log(N/10)

Nx - the number of microorganisms remaining in the test mixture after the preparation has been exposed to it

Na -log of the number of cfu/ml in the test mixture after the preparation has been exposed to

R-logarithm of bacterial cell reduction obtained during the study

The product, tested according to the PN-EN 1650:2019-12 standard, after a contact time of 60 s, a temperature of 20°C, in the presence of a loading substance, shows a fungicidal effect (reduction >4 log) against:

Aspergillus brasiliensis ATCC 16404 at 80% concentration

Candida albicans ATCC 10231 at 80% concentration

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

RB/6256/10/21

The aim of the conducted studies was to assess the biocidal efficacy of the product in relation to the strain Bovine enterovirus Type 1 ATCC VR-796, Porcine respiratory and reproductive syndrome virus (PRRSV) RVB-0209, Influenza A virus (H1N1) ATCC VR-1469. The studies were conducted in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015 item 1926) and in accordance with the European standard PN-EN 14675+2015-06 Chemical disinfectants and antiseptics - Quantitative suspension method for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in veterinary medicine - Test method and requirements (Phase 2/Stage 1).

Incubation 72 h at 37°C ±1°C + 5% CC>2. Number of test repetitions on the microorganism: 1. Test temperature: 10°C ±1 °C. Contact time of the product with the microorganism suspension: 30 min ±10 s and 60 min ±10 s. Incriminating substances: yeast extract 10 g/l + bovine albumin 10 g/l (“dirty” conditions). Product diluent used during the test: Hard water according to PN-EN 14675+2015-06. Stabil

PL 246301 Β1 Product-diluent mixture quality: No precipitate formed during the test. Reference biocidal product: 37% formaldehyde. Method used: neutralization of solutions. Counting method: observation of cytopathic effect, TCID50 endpoint titration using the Spearman-Karber method according to PN-EN 14675+2015-06. Neutralizer used, composition: Eagle minimal medium (MEM). Media used: Eagle minimal medium (MEM) + 10% FBS - medium used to multiply cell lines; Eagle minimal medium (MEM) + 2% FBS - medium used to determine product activity, product cytotoxicity, susceptibility of tested strains to glutaraldehyde, and to determine the concentration of viral strains (the medium was prepared with a concentration of 4% FBS so that the final FBS concentration was 2%). The test mixture was purified from the product in accordance with Appendix B.2 of the PN-EN 14476+A2:2019-08 standard. Efficacy tests against African swine fever ASF were performed on Porcine respiratory and reproductive syndrome virus (PRRSV), which is an alternative to the dangerous strain, in accordance with the work performed at the National Veterinary Institute - National Research Institute, Aleja Partyzantów 57, 24-100 Puławy "Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercially Available Disinfectants" Pathogens 9 (2020) 878.

Identification of microbial strains and cell lines

Virus strain Collection catalog number Cell line Collection catalog number Bovine enterovirus Type 1 (ECBO) ATCC VR-796 BT ATCCCRL-1390 Influenza A virus (H1N1) ATCC VR-1469 MDCK (NBL-2) ATCC CCL-34 PRRSV RVB-0209 MA-104 Clone 1 ATCC CRL-2378.1

Results of validation tests of microbial strains and cell lines

Test organism and And B C D Bovine enterovirus Type 1 (ECBO) N: 8,10 0.21 8,14 1.0 0.08 Influenza A virus (H1N1) N: 844 0.14 8.06 1,2 0.25 PRRSV N :8.02 0.26 8.06 1.8 0.24

α-titer of the virus used for testing, expressed in logarithmic form.

A-difference between the virus changed in the neutralized product and the virus titer. B-control of the virus titer using PBS instead of the tested product.

C-Reduction in viral load after 30 minutes of testing with the reference biocidal product.

D-control of the neutralization efficiency of the tested product

Research results

Test organism and Results for individual concentrations in % by weight of the product (test conditions: temperature: 10°C ± 1°C) 80% 30 min 80% 60 min 0.1% 60 min Bovine enterovirusType 1 (ECBO) N: 8,10 b: <2.00 R: >6.10 b: <2.00 R: >6.10 b: 7.64 R: 0.46 Influenza A virus (Η IN 1) N: 8,14 b: <2.00 R: >5.14 b: <2.00 R: >6.14 b: 7.26 R: 0.88 PRRSV N: 8.02 b: <2.00 R: >6.02 b: <2.00 R: >6.02 b: 7.42 R: 0.60

R-a-b achieved reduction in viral load, b-remaining viral load after contact time t

The product tested according to the PN-EN 14675+2015-06 standard, during 30 min and 60 min, at a temperature of 10°C, in the presence of burdening substances, shows a virus-killing effect (reduction >4 Ig) against:

Bovine enterovirus Type 1 (ECBO)

Influenza A virus (Η1N1)

PRRSV

ATCC VR-796

ATCC VR-1469 RVB-0209 at 80% concentration at 80% concentration at 80% concentration

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

RB/6255/10/21

The aim of the conducted studies was to evaluate the biocidal efficacy of the product against the strains of Poliovirus type 1, LSc 2ab (NIBSC 10/164), Murine norovirus (S99), Adenovirus type 5

PL 246301 Β1 (ATCC VR-5), Human Coronavirus 229E (ATCC VR-740). The tests were performed in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015, item 1926) and in accordance with the European standard PN-EN 14476+A2:2019-08 Chemical disinfectants and antiseptics - Quantitative suspension method for the evaluation of virucidal activity in the medical area - Test method and requirements (Phase 2 / Step 1).

Incubation 72 h at 37°C ±1 °C + 5% CO2. Number of test repetitions on a microorganism: 1. Test temperature: 20°C ±1°C. Contact time of the product with the microbial suspension: 60 s ±10 s, 5 min ±10 s Incident substances: bovine albumin 3 g/l. Product diluent used during the test: Hard water according to PN-EN 14476+A2:2019. Stability of the product mixture with diluent: No precipitate formed during the test. Reference biocidal product: 0.7% (w/v) glutaraldehyde. Method used: neutralization of solutions. Counting method: observation of cytopathic effect, TCID50 endpoint titration Spearman-Karber method according to PN-EN 14476+A2:2019-08. Neutralizer used, composition: Eagle minimal medium (MEM). Media used: Eagle minimal medium (MEM) + 10% FBS - medium used to multiply cell lines; Eagle minimal medium (MEM) + 2% FBS - medium used to determine product activity, product cytotoxicity, susceptibility of tested strains to glutaraldehyde, and to determine the concentration of viral strains (the medium was prepared with a concentration of 4% FBS so that the final FBS concentration was 2%). The test mixture was purified from the product in accordance with appendix B.2 of PN-EN 14476+A2:2019-08.

Identification of microbial strains and cell lines

Virus strain Collection catalog number Cell line Collection catalog number Poliovirus type 1 LSc 2ab NIBSC 10/164 HeLa ATCC CCL-2 Murine norovirus S99 Berlin RAW264.7 ATCCTIB-71 Adenovirus type 5 ATCC VR-5 HeLa ATCC CCL-2 Human coronavirus 229E ATCC VR-740 MRC-5 ATCC CCL-171

Results of validation tests of microbial strains and cell lines

Test organism and And B C D Poliovirus type 1 LSc 2ab 8.48 1% 8.30 1,3 3,1 Murine norovirus 8.44 1% 8.45 2,3 3.5 Adenovirus type 5 8,16 1% 8.20 3.7 5.3 Human coronavirus 229E 8.06 1% 7.94 2.4 3.8

c-titer of the virus used for testing, expressed in logarithmic form.

A-difference between the virus changed in the neutralized product and the virus titer. B-control of the virus titer using PBS instead of the tested product.

C-Reduction in viral load after 30 minutes of testing with the reference biocidal product.

D-control of the neutralization efficiency of the tested product

Research results

Test organism and Results for individual concentrations in % by weight of the product (test conditions: temperature: 10°C + 1°C) 80%, 60 sec 80%, 5 min 0.1%, 5 min Poliovirus type 1 LSc 2ab 8.48 b: <2.00 R: >6.48 b: <2.00 R: >6.48 b: 8.03 R: 0.45 Murine norovirus 8/44 b: <2.00 R: >6.44 b: <2.00 R: >6.44 b: 7.65 R: 0.79 Adenovirus type 5 8,16 b: <2.00 R: >6.16 b: <2.00 R: >6.16 b: 7.35 R: 0.81 Human coronavirus 229E 8.06 b: <2.00 R: >6.06 b: <2.00 R: >6.06 b: 6.95 R: 1.11

R-o-b achieved reduction in viral load, b-remaining viral load after contact time t

The product tested according to the PN-EN 14476+A2:2019-08 standard, during 60 s and 5 min, at a temperature of 20°C, diluted in hard water, in the presence of a loading substance, has a cidal effect against viruses, and against enveloped viruses, including in particular coronaviruses (reduction >4 Ig) against:

Poliovirus type 1 LSc 2ab NIBSC 10/164 at 80% concentration

Murine norovirus S99 Berlin at 80% concentration

PL 246301 Β1

Adenovirus type 5 ATCCVR-5 at 80% concentration

Human coronavirus 229E VR-740 at 80% concentration

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

RB/6281/11/21

The aim of the tests was to assess the biocidal efficacy of the product against the strains Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442, Proteus vulgaris ATCC 13315, Enterococcus hirae ATCC 10541, Salmonella typhimurium ATCC 14028. The tests were performed in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015, item 1926) and in accordance with the European standard PN-EN 14349:2013-05 Chemical disinfectants and antiseptics - Quantitative surface method for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary field on non-porous surfaces, without mechanical action - Test method and requirements (phase 2, stage 2). In accordance with the standard, the agent The disinfectant has a biocidal effect on the strain used if the logarithm of bacterial cell reduction obtained during the test is >4.

Incubation 24 h at 37°C ±1°C. Number of test repetitions on a microorganism: 1. Test temperature: 10°C ±1°C. Mandatory contact time of the product with bacterial suspension: 30 min ±10 s. Incriminating substances: bovine albumin 10 g/l and yeast extract 10 g/l. Diluent used during the test: Hard Water according to PN-EN 14349:2013-05. Stability of the product mixture with diluent: No precipitate formed during the test.

Bacteria Validation Test Results

Test organism Bacterial suspension for testing Neutralizer Toxicity Test Validation study Water test N NT NC Nc Staphylococcus aureus ATCC 6538 10-6: >330 10-7: 41 N: 8.01 NT: 7.78 NC: 7.86 Nc: 7.91 Pseudomonas aeruginosa ATCC 15442 10-6: >330 10-7: 39 S: 7.99 NT: 7.67 NC: 7.72 Nc: 7.71 Proteus vulgaris ATCC 13315 10-6: >330 10-7: 35 N: 7.94 NT: 7.67 NC: 7.69 Nc: 7.76 Enterococcus hirae ATCC 10541 10-6: >330 10-7: 46 N: 8.06 NT: 7.71 NC: 7.75 Nc: 7.78 Salmonello typhimurium ATCC 14028 10-6: >330 10-7: 44 N: 8.04 NT: 7.69 NC: 7.72 Nc: 7.75

N-login of the number of cfu/ml applied to the test surface

B-logio number of cfu/ml on the surface intended for the neutralizer toxicity test

C- logio number of cfu/ml on the surface intended for the validation study;

Nw - logio number of cfu/ml on the surface intended for testing in the control with water

PL 246301 Β1

Bacterial test results

Test organism Nc Results for individual concentrations in % by volume of the product (test conditions: contact time: 5 min, temperature: 20°C ± 1“C) 80% 1% 0.1% Staphylococcus aureus ATCC 6538 7.91 10-0x14 10-1x14 10-0:>330 10-l:>330 10-0:>330 10-l:>330 Na:<2.15 Nts:O Na:>5.52 Nts:>330 Na:>5.52 Nts:>330 R=(Nw -Nd) R: >5.76 R: <3.39 R: <3.39 Pseudomonas aeruginosa ATCC 15442 7.71 10-0x14 10-1x14 10-0:>330 10-l:>330 10-0:>330 10-l:>330 Na:<2.15 Nts:0 Na:>5.52 Nts:>330 Na:>5.52 Nts:>330 R=(Nw -Nd) R: >5.56 R: <3.19 R: <3.19 Proteus yulgaris ATCC 13315 7.76 10-0:<14 10-1x14 10-0:>330 10-l:>330 10-0:>330 10-l:>330 Na:<2.15 Nts:0 Na:>5.52 Nts:>330 Na:>5.52 Nts:>330 R=(Nw -Nd) R: >5.61 R: <3.24 R: <3.24 Enterococcus hirae ATCC 10541 7.78 10-0x14 10-1x14 10-0:>330 10-l:>330 10-0:>330 10-l:>330 Na:<2.15 Nts:0 Na:>5.52 Nts:>330 Na:>5.52 Nts:>330 R=(Nw -Nd) R: >5.63 R: <3.26 R: <3.26 Salmonella typhimurium ATCC 14028 7.78 10-0x14 10-1x14 10-0:>330 10-l:>330 10-0:>330 10-l:>330 Na:<2.15 Nts:O Na:>5.52 Nts:>330 Na:>5.52 Nts:>330 R=(Nw -Nd) R: >5.60 R: <3.23 R: <3.23

Nd - logio number of cfu/ml on the surface intended for testing the effectiveness of the disinfectant R - reduction of the number of microorganisms during the test

Nts - number of cfu remaining on the surface after the test

The product tested according to the PN-EN 14349:2013-05 standard, after a contact time of 30 minutes, at a temperature of 10°C, in the presence of a loading substance, shows a bactericidal effect on surfaces (>> log reduction) against:

Staphylococcus aureus ATCC 6538 at 80% concentration Pseudomonas aeruginosa ATCC 15442 at 80% concentration Proteus vulgaris ATCC 13315 at 80% concentration Enterococcus hirae ATCC 10541 at 80% concentration Salmonella typhimurium ATCC 14028 at 80% concentration

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

RB/6562/01/22

The aim of the conducted tests was to assess the biocidal efficacy of the product against the strain Influenza A virus (H5N1) A/Vietnam/1203/2004. The tests were conducted in accordance with the Act of 9 October 2015 on biocidal products (Journal of Laws 2015, item 1926) and in accordance with the European standard PN-EN 14675+2015-06 Chemical disinfectants and antiseptics - Quantitative suspension method for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in veterinary medicine - Test method and requirements (Phase 2 / Stage 1).

Incubation 72 h at 37°C ±1 °C + 5% CO2. Number of test repetitions on a microorganism: 1. Test temperature: 10°C ±1 °C. Contact time of the product with the microorganism suspension: 30 min ±10 s and 60 min ±10 s. Incident substances: yeast extract 10 g/l + bovine albumin 10 g/l ("dirty" conditions). Product diluent used during the test: sterile demineralized water. Stability of the product-diluent mixture: No precipitate formed during the test. Reference biocidal product: 37% formaldehyde. Method used: neutralization of solutions. Counting method: observation of the cytopathic effect, titration of the TCID50 endpoint using the Spearman-Karber method according to PN-EN 14675+2015-06. Neutralizer used, composition: -Eagle minimal medium (MEM). Substrates used: -Eagle minimal medium (MEM) + 10% FBS - medium used to multiply cell lines, -Eagle minimal medium (MEM) + 2% FBS - medium used to determine the activity of the product, cytotoxicity of the product, susceptibility of the tested strains to formaldehyde, and to determine the concentration of viral strains (the medium was prepared with a concentration of 4% FBS to obtain the final

PL 246301 Β1 FBS concentration was 2%). The test mixture was purified from the product in accordance with Appendix B.4 of the PN-EN 14675:2015-06 standard

Identification of microbial strains and cell lines

Virus strain Collection catalog number Cell line Collection catalog number Influenza A virus (H5N1) A/Vietnam/1203/2004 MDCK (NBL-2) ATCC CCL-34

Results of validation tests of microbial strains and cell lines

Test organism and And B C D Influenza A virus (H1N1) N: 8.24 0.09 8,14 1,3 0.22

c-titer of the virus used for testing, expressed in logarithmic form.

A-difference between the virus changed in the neutralized product and the virus titer

B-control of viral load using PBS instead of the tested product

C-Reduction in viral load after 30 minutes of testing with the reference biocidal product.

D-control of the neutralization efficiency of the tested product

Research results

Test organism and Results for individual concentrations in % by weight of the product (test conditions: temperature: 10°C ± 1°C) 80%, 60 sec 80%, 5 min 0.1%, 5 min Influenza A virus (H5N1) N: 8.24 b: <2.00 R: >5.24 b: <2.00 R: >6.24 b: 7.04 R: 1.20

R-a-b achieved reduction in viral load, h-remaining viral load after contact time t

The product tested according to PN-EN 14675+2015-06, during 30 min and 60 min, temp. 10°C, in the presence of incriminating substances, shows a virus-killing effect (reduction >4 Ig) against: Influenza A virus (H5N1) A/Vietnam/1203/2004 at a concentration of 80%.

The results obtained during all inspections and tests met all the requirements of the methodology and were within the established limits.

Example 19. Independent laboratory tests were carried out on the biocidal effectiveness of the disinfectant preparation in the hand disinfection variant, obtained in Example 6, containing 4.9% hydrogen peroxide, 1% glycerin and showing pH = 3. The tests were carried out at the Department of Virology of the National Institute of Public Health PZH in Warsaw. The aim of the test was to evaluate the ANTMR4 disinfectant in terms of virucidal activity against enveloped viruses, under clean test conditions. The test was performed using a quantitative method in accordance with the PN-EN 14476:2013+A2:2019-08 standard Chemical disinfectants and antiseptics. Quantitative suspension method for determining viral activity in the medical area. Test method and requirements (phase 2 / stage 1). The test was performed according to the guidelines of the above standard for products intended for hygienic hand disinfection by rubbing and for disinfection, in terms of virucidal activity against enveloped viruses. The test was performed using the standard method and the LVP method in clean conditions. The final concentration of the tested, ready-to-use disinfectant was 80% (standard method). Based on the tests, it can be concluded that the tested disinfectant, as a ready-to-use product, has activity in the scope of "virucidal effect against enveloped viruses" in the medical area after a contact time of 1 minute, in clean conditions.

Claims (13)

Patent Claims 1. Liquid disinfectant preparation with antibacterial, antiviral, including against enveloped viruses, antifungal and antiyeast activity, containing hydrogen peroxide as an active substance, deionized water and phosphate ions, showing an acidic reaction, characterized in that it contains: 3-5% by weight of hydrogen peroxide, 0.01-2% by weight of glycerin, 0.01-0.06% by weight of a stabilizing and buffering mixture with the composition: 0.0001-0.01 wt.% H3PO4, 0.005-0.02% by weight On ₄ P2Qy10H2Q, 0.005-0.02% wt. Na2H2P2Q7, 0.005-0.02 wt.% NaNOs, 0.005-0.02 wt.% Na2Sn(QH)6, 0.005-0.02 wt.% Na2SiQs, and 93-95 wt.% demineralized water, and has a pH in the range of 3-5, preferably 3-4. 2. Disinfecting preparation according to claim 1, characterized in that the stabilizing-buffering mixture contains: 0.001-0.01 wt.% HsPQ4, 0.01 wt.% Na4P2Q7^10H2Q, 0.01 wt.% Na2H2P2Q7, 0.01 wt.% NaNQs, 0.01 wt.% Na2Sn(QH)6, 0.01 wt.% Na2SiQs. 3. Disinfecting preparation according to claim 1, characterized in that it additionally contains 0.005-0.02 wt.% of detergent, preferably in the form of a mixture of anionic or nonionic surface-active compounds selected from sodium lauryl sulfate or ethoxylated alcohols with chain lengths in the range of C6-C15, preferably in the range of C10-C15, especially isotridecanol. 4. A disinfecting preparation according to claim 1, characterized in that it contains 4.9% by weight of hydrogen peroxide. 5. Disinfecting preparation according to claim 1, characterized in that in the version for surface disinfection it contains 0.01-0.5% by weight of glycerin, preferably 0.1% by weight of glycerin. 6. A disinfecting preparation according to claim 1, characterized in that in the version for hand disinfection it contains 0.5-2% by weight of glycerin, preferably 1% by weight of glycerin. 7. A method for producing a liquid disinfectant preparation as defined in claims 1-6, characterized in that it consists of two stages, wherein: In the first stage, a concentrate is produced by preliminary preparation of three solutions: - 50-200 g of Na2Sn(QH)6 powder and 1-3 ml of 5% HsPQ4 are added to 900 g of demineralized water, and the tank is left for 24 hours until a white colloidal suspension appears, - 50-200 g of 30% Na2SiQs solution and 1-3 ml of 5% H3PQ4 are added to 900 g of demineralized water, and the tank is left for 24 hours until a white colloidal suspension appears, - 1.33-133 g of 75% H3PQ4 solution, 50-200 g of NaNQ3 powder, 50-200 g of Na4P2Q7^10H2Q powder and 50-200 g of Na2H2P2Q7 powder are added to 2000 g of demineralized water and mixed until the ingredients dissolve, then 100 g of detergent is optionally added, then 450 g of demineralized water and the whole is mixed gently, - then these three solutions are combined to obtain a 5 kg concentrate with a pH in the range of 0.7-2.7, and in the second stage, 5 kg of the obtained concentrate is mixed with 1-20 kg of glycerin and 85.7-142.9 kg of a 35% aqueous solution of hydrogen peroxide and the whole is diluted with deionized water to obtain a 1000 kg preparation with a pH in the range of 3-5, preferably 3-4. 8. A method according to claim 7, characterized in that the concentrate is prepared using a detergent in the form of a mixture of anionic or nonionic surface-active compounds selected from sodium lauryl sulphate or ethoxylated alcohols with chain lengths in the range of C6-C15, preferably in the range of C10-C15, for example isotridecanol. 9. A method according to claim 7, characterized in that 100 g of Na2Sn(QH)6 powder, 100 g of 30% Na2SiQ3 solution, 13.3-133 g of 75% H3PQ4 solution, 100 g of NaNQ3 powder, 100 g of Na4P2Q7^10H2Q powder, 100 g of Na2H2P2Q7 powder and 100 g of detergent are used to prepare the concentrate. 10. The method according to claim 7, characterized in that during dilution of the concentrate 140 kg of a 35% aqueous solution of H2Q2 is added and a preparation with a 4.9% H2Q2 concentration is obtained. 11. The method according to claim 7, characterized in that in the version of the disinfecting preparation for disinfecting surfaces, 1 kg of glycerin is added during dilution of the concentrate. 12. The method according to claim 7, characterized in that in the version of the disinfectant preparation for hand disinfection, 10 kg of glycerin is added during dilution of the concentrate. 13. The method according to claim 7, characterized in that during the preparation and storage of the concentrate and the disinfectant preparation, containers, mixers, tools and pouring lines made exclusively of plastics are used.