PL208416B1 - Application of benzalkonium lactate for washing and disinfection - Google Patents

Description

The subject of the invention is the use of benzalkonium lactate for cleaning and disinfection.

The use of surfactants contributed to the improvement of personal hygiene. We most often use surfactants in the form of water solutions. The type of compound used, and more specifically the ability to lower the surface tension, determine the effectiveness of the cleaning agent. Generally, we divide surfactants into non-ionic and ionic, and the latter may be anionic, cationic and amphoteric. This division results from the structure of the molecule. There are a number of cleaning preparations on the market, while a limited number applies to cleaning and disinfecting preparations.

The subject of the invention is the use of benzalkonium lactate in the form of the L (+), D (-) isomer and the DL racemate, of general formulas 1, 2, 3, in the form of an aqueous solution with a concentration of 0.01% to 80%, for cleaning and disinfecting .

Thanks to the solution according to the invention, the following technical and economic effects were obtained:

- the preparation contains only benzalkonium lactate, it is an aqueous solution of one chemical compound from the group of cationic surfactants,

- the used benzalkonium lactate shows surface activity, is effective against bacteria and fungi, and at the same time has antistatic properties, it is a chemical compound containing a pharmacologically acceptable anion,

- the preparation is colorless, does not stain washed and disinfected surfaces.

The use of water preparations containing an active ingredient showing multifunctional action is pro-ecological. Only one well-defined chemical compound is introduced into the environment. An additional advantage of the invention is the introduction of a pharmacologically acceptable anion, i.e. lactate. The optically active lactates L (+) and D (-) were used, as well as the DL racemate of general formulas 1, 2, 3.

Tables 1 and 2 summarize the determined MIC and MBC values against bacteria and fungi. The MIC values were determined by the serial dilution method.

Measurements of the surface tension were made using the drop shape method at 20 ° C using the Tracker drop shape analyzer by IT Concept. The individual solutions were prepared from a mother liquor with a concentration of 10 ^-2 M by subsequent dilution. The surface activity of the new, aqueous washing-disinfecting preparations according to the invention is illustrated by the following examples:

P r z k ł a d I

Preparation containing D, L-benzalkonium lactate [BA] [D, L-mle]

4.02 g of benzalkonium D, L-lactate [BA] [D, L-mle] were dissolved in 500 cm ^{3 of} tap water. A clear solution was obtained that effectively cleans glass, metal, stone and plastic surfaces (Teflon, PVC). The surface activity of the aqueous solution of benzalkonium D, L-lactate is described by the following parameters: CMC = 3.98x10 ^-3 mol / dm ³ ; ycmc = 37.4 mN / m; Γ = 2.48x10 ^-6 mol / m ² ; A = 6,70x10 ^-19 m ^2; Δ G0dS. = -24.1 kJ / mol.

P r z x l a d II

Preparation containing benzalkonium L-lactate [BA] [D, L-mle]

2.01 g of benzalkonium D, L-lactate [BA] [D, L-mle] were dissolved in 500 cm ³ of demineralized water. A clear solution was obtained that effectively cleans glass, metal, stone and plastic surfaces (Teflon, PVC). The surface activity of the aqueous benzalkonium lactate D, L solution is described by the following parameters: CMC = 5.01x10 ^-3 mol / dm ³ ; ycmc = 38.5 mN / m; Γ = 3.64x10 ^-6 mol / m ² ; A = 4,57x10 ^-19 m ^2; Δ Ga ⁰ ds = -21.9 kJ / mol. Antistatic properties:

surface resistance lg R = 8.9; half-time of charge decay τ = 0.4 s. The measurement was made on a polyethylene film.

Biological research

The activity of benzalkonium lactate against bacteria and fungi was investigated. MIC - the minimum inhibitory concentration was determined by the serial dilution method. MBC - minimum bactericidal concentration determined according to Pernak et al. New J. Chem. 2007, 31, 879-892.

The activity of benzalkonium lactate was assessed against the following bacterial strains: Micrococcus luteus NCTC 7743, Staphylococcus aureus NCTC 4163, Staphylococcus epidermiPL 208 416 B1 dis ATCC 49134, Enterococcus faecium ATCC 49474, Moraxella catarrhalis ATCC90022ensi, ATCC 25CC, ATCC 25CC, ATCC. Proteus vulgaris NCTC 4635, Pseudomonas aeruinosa NCTC 6749, Bacillus subtilis ATCC 6633 and fungi: Candida albicans ATCC 10231, Rhodotorula rubra (Demml 1889, Lodder 1934). The MIC values were determined by the serial dilution method. The MIC values determined are summarized in Table 1 and the MBC values in Table 2. The efficacy of lactate compared to commercial benzalkonium chloride is higher and covers a wide spectrum of microorganisms.

T a b e l a 1. Effect of benzalkonium lactate on bacteria and fungi - MIC minimum inhibitory concentration in ppm

Test strain [BA] [L-mle] [BA] [D, L-mle] [BA] [Cl] * Micrococcus luteus NCTC 7743 1 2 0.5 Staphylococcus aureus NCTC 4163 1 2 1 Staphylococcus epidermidis ATCC 49134 1 1 0.5 Enterococcus faecium ATCC 49474 0.5 2 2 Moraxella catarrhalis ATCC 25238 0.5 2 0.2 Escherichia coli ATCC 25922 0.5 8 1 Serratia marcescens ATCC 8100 62 125 62 Proteus vulgaris NCTC 4635 31 250 31 Pseudomonas aeruginosa NCTC 6749 62 125 62 Bacillus subtilis ATCC 6633 1 1 1 Candida albicans ATCC 10231 2 4 4 Rhodotorula rubra (Demml 1889, Lodder 1934) 2 2 8

* standard, commercial benzalkonium chloride

T a b e l a 2. Effect of benzalkonium lactate on bacteria and fungi - MBC minimum killer concentration in ppm

Test strain [BA] [L-mle] [BA] [D, L-mle] [BA] [Cl] * Micrococcus luteus NCTC 7743 8 16 4 Staphylococcus aureus NCTC 4163 4 2 8 Staphylococcus epidermidis ATCC 49134 4 8 2 Enterococcus faecium ATCC 49474 4 2 8 Moraxella catarrhalis ATCC 25238 4 2 0.5 Escherichia coli ATCC 25922 8 125 1 Serratia marcescens ATCC 8100 62 125 62 Proteus vulgaris NCTC 4635 31 250 31 Pseudomonas aeruginosa NCTC 6749 62 125 62 Bacillus subtilis ATCC 6633 1 1 1 Candida albicans ATCC 10231 8 62 31 Rhodotorula rubra (Demml 1889. Lodder 1934) 4 62 31

* standard, commercial benzalkonium chloride

Claims (1)

The use of benzalkonium lactate in the form of the L (+), D (-) isomer and the DL racemate, with general formulas 1, 2, 3, in the form of an aqueous solution with a concentration of 0.01% to 80%, for cleaning and disinfection.