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PE20251268A1 - Proteiina artificial sensora de actividad e inhibicion de proteasas - Google Patents

Proteiina artificial sensora de actividad e inhibicion de proteasas

Info

Publication number
PE20251268A1
PE20251268A1 PE2023002931A PE2023002931A PE20251268A1 PE 20251268 A1 PE20251268 A1 PE 20251268A1 PE 2023002931 A PE2023002931 A PE 2023002931A PE 2023002931 A PE2023002931 A PE 2023002931A PE 20251268 A1 PE20251268 A1 PE 20251268A1
Authority
PE
Peru
Prior art keywords
biosensor
protease
protease activity
activity
inhibition
Prior art date
Application number
PE2023002931A
Other languages
English (en)
Inventor
Manrique Katherin Lizet Penaranda
Mayer Pohl Luis Milon
Rojas Cesar Gabriel Mendoza
Original Assignee
Univ Peruana De Ciencias Aplicadas S A C
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Peruana De Ciencias Aplicadas S A C filed Critical Univ Peruana De Ciencias Aplicadas S A C
Priority to PE2023002931A priority Critical patent/PE20251268A1/es
Priority to PCT/PE2024/050022 priority patent/WO2025089973A1/es
Publication of PE20251268A1 publication Critical patent/PE20251268A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Se proporciona un biosensor de actividad proteasa fiable para la determinacion de la escision de un sitio de reconocimiento de una proteasa que puede ser visualizado por FRET, cambio de fluorescencia o por electroforesis SDS-PAGE. Este biosensor consiste en un dominio globular, un conector flexible con una zona de reconocimiento/escision proteasa variable y un segundo dominio globular. La propuesta puede ser util para detectar proteasas y evaluar su actividad, como para hallar nuevos inhibidores de proteasas o monitorear la eficacia de agentes terapeuticos en pacientes en tratamiento. El biosensor propuesto es una proteina ingenearizada proveniente de la ingenierizacion racional de proteinas lo que convierte a esta molecula en unica y no presente en la naturaleza.
PE2023002931A 2023-10-24 2023-10-24 Proteiina artificial sensora de actividad e inhibicion de proteasas PE20251268A1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PE2023002931A PE20251268A1 (es) 2023-10-24 2023-10-24 Proteiina artificial sensora de actividad e inhibicion de proteasas
PCT/PE2024/050022 WO2025089973A1 (es) 2023-10-24 2024-10-23 Proteina artificial sensora de actividad e inhibicion de proteasas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PE2023002931A PE20251268A1 (es) 2023-10-24 2023-10-24 Proteiina artificial sensora de actividad e inhibicion de proteasas

Publications (1)

Publication Number Publication Date
PE20251268A1 true PE20251268A1 (es) 2025-05-14

Family

ID=95516726

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2023002931A PE20251268A1 (es) 2023-10-24 2023-10-24 Proteiina artificial sensora de actividad e inhibicion de proteasas

Country Status (2)

Country Link
PE (1) PE20251268A1 (es)
WO (1) WO2025089973A1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI910084A0 (fi) * 1988-07-08 1991-01-07 Smithkline Beckman Corp Retrovirala proteaser bindande peptider.
CN100535123C (zh) * 2001-08-10 2009-09-02 阿赫姆生物系统公司 蛋白酶检测系统
US20050214890A1 (en) * 2003-11-26 2005-09-29 Zhiqun Tan Novel "Cleave-N-Read" system for protease activity assay and methods of use thereof
FR2932190A1 (fr) * 2008-06-10 2009-12-11 Commissariat Energie Atomique Peptides cycliques fluorescents, procedes de preparation de ceux-ci et utilisation de ces peptides pour mesurer l'activite enzymatique d'une enzyme protease
US10962529B1 (en) * 2020-12-01 2021-03-30 King Abdulaziz University Fluorescent probe based biosensor and assay for the detection of SARS-CoV-2

Also Published As

Publication number Publication date
WO2025089973A1 (es) 2025-05-01

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