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PE20251581A1 - Una proteina insecticida mutante vip3 y su aplicacion - Google Patents

Una proteina insecticida mutante vip3 y su aplicacion

Info

Publication number
PE20251581A1
PE20251581A1 PE2025000258A PE2025000258A PE20251581A1 PE 20251581 A1 PE20251581 A1 PE 20251581A1 PE 2025000258 A PE2025000258 A PE 2025000258A PE 2025000258 A PE2025000258 A PE 2025000258A PE 20251581 A1 PE20251581 A1 PE 20251581A1
Authority
PE
Peru
Prior art keywords
vip3
application
mutant
host cell
expression
Prior art date
Application number
PE2025000258A
Other languages
English (en)
Inventor
Lei Lian
Yongchun Wu
Bo Chen
Huarong Li
Original Assignee
Qingdao Kingagroot Seed Science Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Kingagroot Seed Science Co Ltd filed Critical Qingdao Kingagroot Seed Science Co Ltd
Publication of PE20251581A1 publication Critical patent/PE20251581A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/60—Malvaceae, e.g. cotton or hibiscus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion se refiere a una proteina insecticida mutante Vip3; asi como el polinucleotido que codifica dicha proteina, optimizado para que se exprese en celulas de plantas. Tambien se refiere al vector que contiene el polinucleotido y un elemento regulador de la expresion operativamente unido al mismo; a la celula huesped que contiene el polinucleotido o el vector de expresion, con preferencia, la celula huesped es una celula de planta. La aplicacion del vector de expresion o la celula huesped mejora las caracteristicas de resistencia a los insectos de una planta.
PE2025000258A 2022-09-01 2023-08-31 Una proteina insecticida mutante vip3 y su aplicacion PE20251581A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202211069297 2022-09-01
CN202310225662 2023-03-09
PCT/CN2023/116207 WO2024046429A1 (zh) 2022-09-01 2023-08-31 一种突变杀虫蛋白Vip3及其应用

Publications (1)

Publication Number Publication Date
PE20251581A1 true PE20251581A1 (es) 2025-06-16

Family

ID=90025932

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2025000258A PE20251581A1 (es) 2022-09-01 2023-08-31 Una proteina insecticida mutante vip3 y su aplicacion

Country Status (8)

Country Link
CN (1) CN117624314A (es)
AU (1) AU2023332832A1 (es)
CA (1) CA3262019A1 (es)
CL (1) CL2025000540A1 (es)
CO (1) CO2025003941A2 (es)
MX (1) MX2025002417A (es)
PE (1) PE20251581A1 (es)
WO (1) WO2024046429A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119775374B (zh) * 2025-01-02 2025-12-09 江苏海洋大学 一种活性提高的Vip3蛋白及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA116881C2 (uk) * 2012-02-16 2018-05-25 Сінгента Партісіпейшнс Аг Сконструйований пестицидний білок vip3
NZ727213A (en) * 2012-03-09 2020-03-27 Vestaron Corp Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
JP6106263B2 (ja) * 2012-04-06 2017-03-29 モンサント テクノロジー エルエルシー 半翅目昆虫種に対して毒性のタンパク質
WO2017128039A1 (zh) * 2016-01-26 2017-08-03 浙江大学 基因组合及其用途
BR112020021445A2 (pt) * 2018-04-20 2021-01-19 AgBiome, Inc. Genes pesticidas e métodos de uso
CN110903361B (zh) * 2019-12-24 2021-08-06 隆平生物技术(海南)有限公司 一种植物抗虫基因mVip3Aa及其载体和应用

Also Published As

Publication number Publication date
MX2025002417A (es) 2025-04-02
WO2024046429A1 (zh) 2024-03-07
AU2023332832A1 (en) 2025-01-30
CL2025000540A1 (es) 2025-07-18
CA3262019A1 (en) 2025-06-13
CO2025003941A2 (es) 2025-05-08
CN117624314A (zh) 2024-03-01

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