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PE20180045A1 - Dispositivo, plataforma y ensayo para evaluacion de celulas - Google Patents

Dispositivo, plataforma y ensayo para evaluacion de celulas

Info

Publication number
PE20180045A1
PE20180045A1 PE2017001425A PE2017001425A PE20180045A1 PE 20180045 A1 PE20180045 A1 PE 20180045A1 PE 2017001425 A PE2017001425 A PE 2017001425A PE 2017001425 A PE2017001425 A PE 2017001425A PE 20180045 A1 PE20180045 A1 PE 20180045A1
Authority
PE
Peru
Prior art keywords
factor
cells
factors
migration
adherence
Prior art date
Application number
PE2017001425A
Other languages
English (en)
Inventor
Cox Juan Pablo Acevedo
Original Assignee
Cells For Cells S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cells For Cells S A filed Critical Cells For Cells S A
Publication of PE20180045A1 publication Critical patent/PE20180045A1/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0694—Creating chemical gradients in a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se refiere a un dispositivo para el analisis de una poblacion de celulas, caracterizado porque comprende de una camara de carga celular; al menos dos carriles o lineas de ensayo paralelas separadas a igual distancia entre si, cada carril o linea de ensayo comprende un hidrogel; al menos dos canales de ensayo paralelos. Estos dispositivos son capaces de evaluar la respuesta de migracion en la presencia de un gradiente estable encapsulado de un factor o combinacion de factores, y cuantificar la respuesta de adherencia dentro de los microcanales, en la presencia de diferentes factores; la gradiente de factor encapsulado comprende un rango de concentracion del factor de induccion de migracion o adherencia desde aproximadamente 1nM a 400 nM, en donde el factor de liberacion es una proteasa, la colagenasa; las celulas son seleccionadas del grupo que consiste en celulas madre, precursoras, celulas Muse-AT, hematopoyeticas, entre otras. Este dispositivo puede ser utilizado para evaluar la migracion celular o adherencia en la presencia de factores que podrian estar relacionados con la tasa de exito en diferentes tratamientos, tales como recuperacion del dano renal u otras patologias; y permite que esta informacion sea utilizada para evaluar el potencial terapeutico.
PE2017001425A 2015-02-20 2016-02-22 Dispositivo, plataforma y ensayo para evaluacion de celulas PE20180045A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2015/051317 WO2016132182A1 (en) 2015-02-20 2015-02-20 Devices for studying chemotaxis/adherence in cells wherein gradients and channels are formed using hydrogels

Publications (1)

Publication Number Publication Date
PE20180045A1 true PE20180045A1 (es) 2018-01-15

Family

ID=52684599

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2017001425A PE20180045A1 (es) 2015-02-20 2016-02-22 Dispositivo, plataforma y ensayo para evaluacion de celulas

Country Status (7)

Country Link
US (1) US10766029B2 (es)
EP (1) EP3259066A4 (es)
JP (1) JP2018505691A (es)
CL (1) CL2017002101A1 (es)
MX (1) MX2017010569A (es)
PE (1) PE20180045A1 (es)
WO (2) WO2016132182A1 (es)

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EP3519828A4 (en) * 2016-09-29 2020-06-03 Animantis, LLC METHOD AND DEVICE FOR EVALUATING IMMUNE SYSTEM ACTIVITY AND THERAPEUTIC EFFECTIVENESS
CN108619575A (zh) * 2018-06-15 2018-10-09 天津工业大学 一种可缓释生长因子的表层分子印迹高强度水凝胶膜的制备方法
CN109358009B (zh) * 2018-10-26 2021-08-10 武汉百德瑞康生物技术有限公司 胱抑素c测定试剂盒及其制备方法和检测方法
CN111695389B (zh) * 2019-03-15 2023-06-20 北京四维图新科技股份有限公司 一种车道线聚类方法及装置
CA3160257A1 (en) * 2019-12-02 2021-06-10 Ho Nam Chan A chemical delivery system, device and method thereof
CN111138723B (zh) * 2019-12-10 2021-07-20 四川农业大学 一种3d打印梯度抗菌膜的制备方法、产品及其应用
CO2020015994A1 (es) 2020-12-18 2022-06-21 Inst Distrital De Ciencia Biotecnologia E Innovacion En Salud Idcbis Método in vitro para evaluación funcional de progenitores hematopoyéticos
CN112903637B (zh) * 2021-01-18 2022-06-03 江西英大生物技术有限公司 尿胰蛋白酶原-2的检测试剂盒、适配的检测装置及检测方法
CN114836291B (zh) * 2021-02-01 2025-11-21 深圳拜尔洛克生物技术有限公司 生物材料解冻复苏的系统及其方法
CN215775135U (zh) * 2021-03-03 2022-02-11 深圳拜尔洛克生物技术有限公司 用于生物组织冷冻保存或解冻复苏的芯片
CN113373198A (zh) * 2021-06-17 2021-09-10 上海睿钰生物科技有限公司 一种确定细胞的迁移能力的方法和系统
WO2023144747A1 (en) * 2022-01-28 2023-08-03 Glaxosmithkline Intellectual Property Development Limited High-throughput assay for cell migration, chemotaxis, and function

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US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US6699665B1 (en) * 2000-11-08 2004-03-02 Surface Logix, Inc. Multiple array system for integrating bioarrays
JP2005523710A (ja) * 2002-04-24 2005-08-11 サーフェイス ロジックス，インコーポレイティド 白血球遊走のモニター装置及び方法
US7857849B2 (en) * 2004-10-05 2010-12-28 The Board Of Trustees Of The Leland Stanford Junior Iniversity Artificial corneal implant
US20090136982A1 (en) * 2005-01-18 2009-05-28 Biocept, Inc. Cell separation using microchannel having patterned posts
US8685638B2 (en) * 2005-01-20 2014-04-01 The Regents Of The University Of California Cellular microarrays for screening differentiation factors
WO2009102453A2 (en) * 2008-02-11 2009-08-20 The General Hospital Corporation System and method for quantitative assessment of biological migration behavior
CA2720261C (en) * 2008-04-08 2020-09-22 Massachusetts Institute Of Technology Three-dimensional microfluidic platforms and methods of use thereof
JP5228826B2 (ja) * 2008-11-17 2013-07-03 大日本印刷株式会社 細胞画像解析装置および細胞画像解析方法
WO2010108095A2 (en) * 2009-03-19 2010-09-23 The General Hospital Corporation Microfluidic cell motility assay
CN103477222B (zh) * 2010-09-29 2016-08-10 麻省理工学院 用于高通量研究细胞相互作用的装置
EP2714884B1 (en) 2011-05-27 2019-08-21 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
KR101311325B1 (ko) * 2012-09-13 2013-09-30 이상재 합성 디자인된 3차원 미세환경 구조물

Also Published As

Publication number Publication date
WO2016132221A2 (en) 2016-08-25
EP3259066A2 (en) 2017-12-27
WO2016132182A1 (en) 2016-08-25
US20180236442A1 (en) 2018-08-23
WO2016132221A3 (en) 2016-10-13
MX2017010569A (es) 2018-07-06
EP3259066A4 (en) 2018-12-05
CL2017002101A1 (es) 2018-03-23
US10766029B2 (en) 2020-09-08
JP2018505691A (ja) 2018-03-01

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