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PE20110562A1 - Produccion y almacenamiento mejorados de proteinas en plantas - Google Patents

Produccion y almacenamiento mejorados de proteinas en plantas

Info

Publication number
PE20110562A1
PE20110562A1 PE2010001207A PE2010001207A PE20110562A1 PE 20110562 A1 PE20110562 A1 PE 20110562A1 PE 2010001207 A PE2010001207 A PE 2010001207A PE 2010001207 A PE2010001207 A PE 2010001207A PE 20110562 A1 PE20110562 A1 PE 20110562A1
Authority
PE
Peru
Prior art keywords
seed
deficiency
protein
seed storage
proteins
Prior art date
Application number
PE2010001207A
Other languages
English (en)
Inventor
Eliot M Herman
Monica S Schmidt
Original Assignee
Donald Danforth Plant Sci Ct
Us Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Donald Danforth Plant Sci Ct, Us Agriculture filed Critical Donald Danforth Plant Sci Ct
Publication of PE20110562A1 publication Critical patent/PE20110562A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8234Seed-specific, e.g. embryo, endosperm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

REFERIDA A UNA PLANTA TRANSGENICA, CARACTERIZADA PORQUE COMPRENDE: A) UNA DEFICIENCIA DE UNA O MAS PROTEINAS DE ALMACENAMIENTO DE SEMILLA, DONDE LA DEFICIENCIA SE TRADUCE EN UNA REDUCCION DE LA MENOS DE AL MENOS 50% EN PROTEINA DE ALMACENAMIENTO DE SEMILLA ENDOGENA EN COMPARACION CON AQUELLA SILVESTRE; B) UN POLINUCLEOTIDO HETEROLOGO QUE COMPRENDE UN PROMOTOR DE PROTEINA DE ALMACENAMIENTO DE SEMILLA, UN MARCO DE LECTURA ABIERTO QUE COMPRENDE UNA SECUENCIA DE SENAL ER ENLAZADO OPERABLEMENTE AL PROMOTOR DE PROTEINA DE ALMACENAMIENTO DE SEMILLA. DICHA PLANTA TRANSGENICA ES CAPAZ DE PRODUCIR UNA PROTEINA HETEROLOGA A UN NIVEL QUE ES MAYOR A 5% DEL PESO SECO TOTAL DE LA SEMILLA. DICHA SEMILLA ES SOYA Y LA DEFICIENCIA DE PROTEINA DE ALMACENAMIENTO ES DE UNO O MAS DE INHIBIDOR DE TRIPSINA KUNITZ, LECITINA DE SOYA, PROTEINA DE UNION A GLUCOSA, ENTRE OTROS.
PE2010001207A 2008-06-28 2009-06-29 Produccion y almacenamiento mejorados de proteinas en plantas PE20110562A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7661608P 2008-06-28 2008-06-28

Publications (1)

Publication Number Publication Date
PE20110562A1 true PE20110562A1 (es) 2011-08-11

Family

ID=41444990

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2010001207A PE20110562A1 (es) 2008-06-28 2009-06-29 Produccion y almacenamiento mejorados de proteinas en plantas

Country Status (20)

Country Link
US (1) US20100313307A1 (es)
EP (1) EP2307547A4 (es)
JP (1) JP2011526155A (es)
KR (1) KR20110044211A (es)
CN (1) CN102137932A (es)
AP (1) AP2011005557A0 (es)
AR (1) AR072391A1 (es)
AU (1) AU2009261943A1 (es)
BR (1) BRPI0914824A2 (es)
CA (1) CA2729375A1 (es)
CL (1) CL2010001598A1 (es)
CO (1) CO6341485A2 (es)
CU (1) CU20100263A7 (es)
DO (1) DOP2010000399A (es)
EA (1) EA201170104A1 (es)
EC (1) ECSP11010793A (es)
IL (1) IL210210A0 (es)
MX (1) MX2010014541A (es)
PE (1) PE20110562A1 (es)
WO (2) WO2009158694A1 (es)

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JP5809810B2 (ja) * 2011-02-22 2015-11-11 本田技研工業株式会社 タンパク質の植物細胞内への蓄積方法
CA2859490A1 (en) 2012-04-05 2013-10-10 Basf Plant Science Company Gmbh Fungal resistant plants expressing hydrophobin
BR112015009201A2 (pt) * 2012-10-31 2017-11-14 Danisco Us Inc composição compreendeendo um polipeptídeo recombinante, ácido nucleico codificando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
CN106480089A (zh) * 2016-12-30 2017-03-08 上海交通大学 一种提高大豆含硫氨基酸含量及降低过敏原蛋白的方法
CN109486851B (zh) * 2018-10-12 2022-04-01 武汉禾元生物科技股份有限公司 一种提高胚乳生物反应器中重组蛋白表达水平的方法
CN109220805A (zh) * 2018-11-05 2019-01-18 贵州大学 一种红豆树组培苗瓶外生根方法
IL265841A (en) * 2019-04-03 2020-10-28 Yeda Res & Dev A plant that expresses animal milk proteins
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CN110122333A (zh) * 2019-06-17 2019-08-16 西安同人五凤农业有限公司 一种凤凰木种子促进发芽生根的方法
WO2020256372A1 (ko) 2019-06-17 2020-12-24 주식회사 바이오앱 아프리카 돼지열병의 진단을 위한 항원 생산용 재조합 벡터 및 이의 용도
US11326176B2 (en) * 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
US12359212B2 (en) 2019-11-22 2025-07-15 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
US20230380373A1 (en) * 2020-10-28 2023-11-30 Pioneer Hi-Bred International, Inc. Leghemoglobin in soybean
WO2022093977A1 (en) * 2020-10-30 2022-05-05 Fortiphyte, Inc. Pathogen resistance in plants
KR102630105B1 (ko) * 2020-11-26 2024-01-29 전남대학교산학협력단 Cel12A 단백질을 포함하는 셀룰로오스 분해용 조성물 및 이의 제조방법
WO2023027402A1 (ko) 2021-08-27 2023-03-02 주식회사 바이오앱 아프리카 돼지열병 바이러스 유래 항원 단백질을 포함하는 아프리카 돼지열병의 예방용 백신
WO2023076272A1 (en) * 2021-10-25 2023-05-04 The Regents Of The University Of California Vectors and methods for improved dicot plant transformation frequency
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
KR20250014003A (ko) * 2023-07-18 2025-02-03 메디프로젠 주식회사 저장단백질 함량이 저감된 콩 형질전환체 및 이의 용도
KR102726138B1 (ko) 2023-10-17 2024-11-14 대한민국 백시니아 바이러스 재조합 a33r 단백질 및 이를 포함하는 오르소폭스바이러스 항체 진단 키트

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Also Published As

Publication number Publication date
CL2010001598A1 (es) 2011-07-15
ECSP11010793A (es) 2011-07-29
AP2011005557A0 (en) 2011-02-28
BRPI0914824A2 (pt) 2015-12-01
MX2010014541A (es) 2011-07-29
WO2009158716A1 (en) 2009-12-30
CA2729375A1 (en) 2009-12-30
EP2307547A4 (en) 2011-06-22
CN102137932A (zh) 2011-07-27
EP2307547A1 (en) 2011-04-13
WO2009158694A1 (en) 2009-12-30
KR20110044211A (ko) 2011-04-28
CO6341485A2 (es) 2011-11-21
EA201170104A1 (ru) 2011-08-30
DOP2010000399A (es) 2012-11-15
JP2011526155A (ja) 2011-10-06
CU20100263A7 (es) 2012-06-21
AU2009261943A1 (en) 2009-12-30
US20100313307A1 (en) 2010-12-09
AR072391A1 (es) 2010-08-25
IL210210A0 (en) 2011-03-31

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