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PE20020535A1 - ASPERGILLUS OCHRACEUS 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE - Google Patents

ASPERGILLUS OCHRACEUS 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE

Info

Publication number
PE20020535A1
PE20020535A1 PE2001001080A PE2001001080A PE20020535A1 PE 20020535 A1 PE20020535 A1 PE 20020535A1 PE 2001001080 A PE2001001080 A PE 2001001080A PE 2001001080 A PE2001001080 A PE 2001001080A PE 20020535 A1 PE20020535 A1 PE 20020535A1
Authority
PE
Peru
Prior art keywords
oxidoreductase
refers
alpha hydroxylase
alpha
fragments
Prior art date
Application number
PE2001001080A
Other languages
Spanish (es)
Inventor
Suzanne L Bolten
Robert Clayton
Alan M Easton
Leslie C Engel
Dean M Messing
John S Ng
Beverly Reitz
Mark C Walker
Ping T Wang
Original Assignee
Searle Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle Llc filed Critical Searle Llc
Publication of PE20020535A1 publication Critical patent/PE20020535A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C12P33/10—Hydroxylating at 11 position at 11 alpha-position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Rheumatology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pain & Pain Management (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE REFIERE A UNA ENZIMA CITOCROMO P450-COMO (Aspergillus ochraceus 11 ALFA HIDROXILASA) Y UNA OXIDOREDUCTASA (Aspergillus ochraceu OXIDOREDUCTASA) AISLADA A PARTIR DE UN cADN GENERADA A PARTIR DE mARN DE ESPORAS DE Aspergillus ochraceus. CUANDO SE CODIFICA EL cADN EL 11 ALFA HIDROXILASA ES CO-EXPRIMIDO EN LAS CELULAS DE INSECTOS Spodoptera frugiperda (Sf-9) CON EL cADN QUE CODIFICA LA OXIDOREDUCTASA HUMANA COMO UN ELECTRO DONANTE, ESTA CATALIZA EXITOSAMENTE LA CONVERSION SUBSTRATO ESTEROIDE 4-ANDROSTENE-3,17-DIONA (AD) A UN 11 ALFA-HIDROXI-AD DETERMINADO POR HPLC. SE REFIERE TAMBIEN A MOLECULAS ASOCIADAS CON ACIDO NUCLEICO O DERIVADAS A PARTIR DE ESTOS cADNs INCLUYENDO COMPLEMENTOS HOMOLOGOS, FRAGMENTOS Y METODOS QUE USAN ESTAS MOLECULAS DE ACIDO NUCLEICO PARA GENERAR POLIPEPTIDO Y FRAGMENTOS. SE REFIERE TAMBIEN A LA GENERACION DE ANTICUERPOS QUE RECONOCE EL A. ochraceus 11 ALFA HIDROXILASA Y OXIDOREDUCTASA Y METODOS QUE USAN ESTOS ANTICUERPOS PARA DETECTAR LA PRESENCIA DENTRO DE CELULAS HUESPED SIN MODIFICARLAS. LA INVENCION PROVEE UNA NUEVA ENZIMA 11 ALFA HIDROXILASA Y OXIDOREDUCTASA, SUS ACIDOS NUCLEICOS, PROTEINAS, PEPTIDOS, FRAGMENTOS Y HOMOLOGOS. SE REFIERE A DEMAS A METODOS DE IDENTIFICACION Y CLONACION DE OTRAS ENZIMAS INVOLUCRADAS EN EL METABOLISMO ESTEROIDEREFERS TO A CYTOCHROME P450-AS ENZYME (Aspergillus ochraceus 11 ALPHA HYDROXYLASE) AND AN OXIDOREDUCTASE (Aspergillus ochraceu OXIDOREDUCTASE) ISOLATED FROM A cDNA GENERATED FROM ESPORCH FROM MARN. WHEN THE cDNA IS CODED THE 11 ALPHA HYDROXYLASE IS CO-EXPRESSED IN THE INSECT CELLS Spodoptera frugiperda (Sf-9) WITH THE cDNA THAT CODES THE HUMAN OXIDOREDUCTASE AS A DONOR ELECTRO, THIS SUCCESSFULLY SUCCESSFUL 4-SUCCESSFUL CATALYZE 3-CONCERN , 17-DIONA (AD) AT 11 ALPHA-HYDROXY-AD DETERMINED BY HPLC. IT ALSO REFERS TO MOLECULES ASSOCIATED WITH NUCLEIC ACID OR DERIVED FROM THESE cDNAs INCLUDING HOMOLOGICAL COMPLEMENTS, FRAGMENTS AND METHODS THAT USE THESE NUCLEIC ACID MOLECULES TO GENERATE POLYPEPTIDE AND FRAGMENTS. IT ALSO REFERS TO THE GENERATION OF ANTIBODIES RECOGNIZED BY A. ochraceus 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE AND METHODS USED BY THESE ANTIBODIES TO DETECT THE PRESENCE INSIDE HOST CELLS WITHOUT MODIFYING THEM. THE INVENTION PROVIDES A NEW ENZYME 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE, THEIR NUCLEIC ACIDS, PROTEINS, PEPTIDES, FRAGMENTS AND APPROVALS. REFERS TO OTHER METHODS OF IDENTIFICATION AND CLONING OF OTHER ENZYMES INVOLVED IN STEROID METABOLISM

PE2001001080A 2000-10-30 2001-10-30 ASPERGILLUS OCHRACEUS 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE PE20020535A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24430000P 2000-10-30 2000-10-30

Publications (1)

Publication Number Publication Date
PE20020535A1 true PE20020535A1 (en) 2002-06-05

Family

ID=22922180

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2001001080A PE20020535A1 (en) 2000-10-30 2001-10-30 ASPERGILLUS OCHRACEUS 11 ALPHA HYDROXYLASE AND OXIDOREDUCTASE

Country Status (12)

Country Link
US (2) US7033807B2 (en)
EP (1) EP1352054A2 (en)
JP (1) JP2005512502A (en)
CN (1) CN1531590A (en)
AR (1) AR031169A1 (en)
AU (1) AU2002241768A1 (en)
BR (1) BR0115245A (en)
CA (1) CA2427615A1 (en)
IL (1) IL155529A0 (en)
MX (1) MX245674B (en)
PE (1) PE20020535A1 (en)
WO (1) WO2002046386A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2342305T3 (en) * 2004-08-12 2010-07-05 Nippon Suisan Kaisha, Ltd. METHOD OF PRODUCTION OF POLYINSATURATED FATTY ACIDS USING A NEW CELL CONSERVATION TECHNIQUE.
MX2007008773A (en) * 2005-01-25 2007-09-12 Bayer Cropscience Sa Method of analyzing phosphorous acid, fosetyl-al or both simultaneously.
CN102827913B (en) * 2012-08-31 2014-01-15 湖南成大生物科技有限公司 Method for preparing 11 alpha-OH-ADD by mixed fermentation of microbes
CN103627721B (en) * 2013-10-22 2015-08-05 浙江工业大学 G6PDH gene is improving bread mould to the application in steroidal C11 'alpha '-hydroxylation ability and bacterial strain
SI3495482T1 (en) * 2014-01-21 2021-03-31 Synplogen Co., Ltd. The process of preparing a DNA unit assembly and the process of making a DNA concatemer
CN104388515A (en) * 2014-11-28 2015-03-04 江西赣亮医药原料有限公司 Method for producing 11alpha-OH-ADD from phytosterol by mixed fermentation
CN105039470B (en) * 2015-08-03 2018-10-09 华南理工大学 A kind of electron donor albumen of Lactobacillus casei degrading nitrite and its preparation method and application
ES2648614B1 (en) * 2016-05-30 2018-10-15 Consejo Superior De Investigaciones Científicas (Csic) PRODUCTION OF STEROIDS 11-ALFA HYDROXYLATES THROUGH BIOTRANSFORMATION WITH RECOMBINANT BACTERIA
US20190264253A1 (en) * 2016-10-19 2019-08-29 Molecular Warehouse, Ltd. Ultrasensitive electrochemical biosensors
WO2018077944A2 (en) * 2016-10-27 2018-05-03 Bayer Aktiengesellschaft 4,5-annulated 1,2,4-triazolones
WO2020018729A1 (en) * 2018-07-17 2020-01-23 Conagen Inc. Biosynthetic production of gamma-lactones
US11574832B2 (en) * 2018-09-05 2023-02-07 Assembléon B.V. Die attach systems including a verification substrate
CN109182143B (en) * 2018-09-28 2021-09-24 河南农业大学 A kind of Aspergillus fumigatus LSD-1 and its cultivation method and application
WO2020097588A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110499254B (en) * 2019-07-22 2022-05-06 吉林大学 Saline-alkali-resistant aspergillus ochraceus strain W1, and microbial inoculum and application thereof
CN110343622B (en) * 2019-07-26 2021-01-01 兰州理工大学 Cellulase-producing Glycyrrhiza endophytes and their application in extracting glycyrrhizic acid from Glycyrrhiza
CN110713941A (en) * 2019-10-22 2020-01-21 上海应用技术大学 High-expression cytochrome P450 monooxygenase aspergillus ochraceus strain as well as construction method and application thereof
CA3158430A1 (en) * 2019-10-25 2021-04-29 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN113583984B (en) * 2021-05-28 2023-05-23 湖北大学 Cytochrome P450 monooxygenase CYP109B2 and application thereof
CN114456952B (en) * 2022-02-22 2023-04-14 昆明理工大学 A kind of chrysosporium and application thereof
CN115074293B (en) * 2022-07-16 2024-05-28 山东金洋药业有限公司 Glycerol glucoside purification process
CN116240184A (en) * 2023-02-13 2023-06-09 上海交通大学 Construction and application of steroid C14 alpha hydroxylase CLCYP14 mutant

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4419446A (en) * 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4559332A (en) * 1983-04-13 1985-12-17 Ciba Geigy Corporation 20-Spiroxanes and analogues having an open ring E, processes for their manufacture, and pharmaceutical preparations thereof
US4588683A (en) * 1984-02-06 1986-05-13 Eastman Kodak Company Method of preparing 11β, 17α, 20, 21-tetrahydroxy steroids and corresponding 11β, 17α, 21-trihydroxy-20-oxo steroids
US4935233A (en) * 1985-12-02 1990-06-19 G. D. Searle And Company Covalently linked polypeptide cell modulators
US5869283A (en) * 1988-05-06 1999-02-09 Roussel Uclaf Expression cassette operable in a recombinant host
EP0528906B1 (en) 1990-04-30 1995-10-11 The Board Of Regents, The University Of Texas System STEROID 5$g(a)-REDUCTASES
US5384253A (en) * 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5348886A (en) * 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
EP0900283B1 (en) * 1995-12-12 2002-04-03 Akzo Nobel N.V. MICROBIAL 11alpha-HYDROXYLATION OF STEROIDS
BR9714510A (en) 1996-12-11 2000-11-28 Searle & Co Process and preparation of 9,11-epoxy steroids and useful intermediates
SE0302534L (en) 2003-09-23 2005-03-08 Haldex Brake Prod Ab evidence Spring

Also Published As

Publication number Publication date
CA2427615A1 (en) 2002-06-13
CN1531590A (en) 2004-09-22
US7033807B2 (en) 2006-04-25
AR031169A1 (en) 2003-09-10
EP1352054A2 (en) 2003-10-15
IL155529A0 (en) 2003-11-23
US7238507B2 (en) 2007-07-03
MXPA03003809A (en) 2003-07-28
US20030148420A1 (en) 2003-08-07
BR0115245A (en) 2005-05-10
WO2002046386A2 (en) 2002-06-13
AU2002241768A1 (en) 2002-06-18
WO2002046386A3 (en) 2003-08-07
JP2005512502A (en) 2005-05-12
MX245674B (en) 2007-05-07
US20050003473A1 (en) 2005-01-06

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