LV10307B - Molecular cloning and expression of genes encoding proteolytic enzymes - Google Patents

Molecular cloning and expression of genes encoding proteolytic enzymes Download PDF

Info

Publication number: LV10307B
Authority: LV; Latvia
Prior art keywords: bacillus; host cell; dna; cell; serine protease
Prior art date: 1987-02-27

Application number

LVP-93-1390A

Other languages

English (en)

Latvian (lv)

Other versions

LV10307A (lv

Inventor

Eekelen Christiaan A G Van

Der Laan Johannes C Van

Leonardus J S M Mulleners

Original Assignee

Gist Brocades Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-02-27

Filing date

1993-12-29

Publication date

1995-08-20

1993-12-29 Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv

1994-10-20 Publication of LV10307A publication Critical patent/LV10307A/xx

1995-08-20 Publication of LV10307B publication Critical patent/LV10307B/en

Links

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229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
238000013519 translation Methods 0.000 description 1
239000007195 tryptone soya broth Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Mycology (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

LVP-93-1390A 1987-02-27 1993-12-29 Molecular cloning and expression of genes encoding proteolytic enzymes LV10307B (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP87200358		1987-02-27
PCT/NL1988/000007 WO1988006624A2 (fr)	1987-02-27	1988-02-26	Clonage et expression moleculaire de genes codant des enzymes proteolytiques

Publications (2)

Publication Number	Publication Date
LV10307A LV10307A (lv)	1994-10-20
LV10307B true LV10307B (en)	1995-08-20

Family

ID=8197584

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
LVP-93-1390A LV10307B (en)	1987-02-27	1993-12-29	Molecular cloning and expression of genes encoding proteolytic enzymes

Country Status (19)

Country	Link
US (1)	US5217878A (fr)
JP (1)	JP3026567B2 (fr)
KR (1)	KR0127560B1 (fr)
CN (1)	CN88101681A (fr)
AT (2)	ATE181110T1 (fr)
AU (1)	AU620026B2 (fr)
BG (1)	BG49049A3 (fr)
BR (1)	BR8805647A (fr)
CA (1)	CA1339100C (fr)
DE (2)	DE3856331T2 (fr)
DK (2)	DK175738B1 (fr)
FI (3)	FI105483B (fr)
HU (1)	HU213220B (fr)
LT (1)	LT4000B (fr)
LV (1)	LV10307B (fr)
NO (1)	NO884423D0 (fr)
PT (1)	PT86864B (fr)
RU (1)	RU2023723C1 (fr)
WO (1)	WO1988006624A2 (fr)

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ES2562919T3 (es)	2007-08-06	2016-03-09	University Of Chile	Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío
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1988
- 1988-02-26 JP JP63502472A patent/JP3026567B2/ja not_active Expired - Lifetime
- 1988-02-26 WO PCT/NL1988/000007 patent/WO1988006624A2/fr not_active Ceased
- 1988-02-26 PT PT86864A patent/PT86864B/pt not_active IP Right Cessation
- 1988-02-26 BR BR888805647A patent/BR8805647A/pt not_active IP Right Cessation
- 1988-02-26 KR KR1019880701347A patent/KR0127560B1/ko not_active Expired - Fee Related
- 1988-02-26 HU HU881832A patent/HU213220B/hu not_active IP Right Cessation
- 1988-02-26 AU AU13980/88A patent/AU620026B2/en not_active Expired
- 1988-02-27 CN CN198888101681A patent/CN88101681A/zh active Pending
- 1988-02-29 US US07/162,184 patent/US5217878A/en not_active Expired - Lifetime
- 1988-02-29 CA CA000560126A patent/CA1339100C/fr not_active Expired - Lifetime
- 1988-02-29 AT AT91203297T patent/ATE181110T1/de not_active IP Right Cessation
- 1988-02-29 AT AT88200377T patent/ATE180016T1/de not_active IP Right Cessation
- 1988-02-29 DE DE3856331T patent/DE3856331T2/de not_active Expired - Lifetime
- 1988-02-29 DE DE3856339T patent/DE3856339T2/de not_active Expired - Lifetime
- 1988-10-05 NO NO884423A patent/NO884423D0/no unknown
- 1988-10-24 FI FI884904A patent/FI105483B/fi active IP Right Grant
- 1988-10-25 BG BG085831A patent/BG49049A3/xx unknown
- 1988-10-26 RU SU884356848A patent/RU2023723C1/ru active
- 1988-10-26 DK DK198805939A patent/DK175738B1/da not_active IP Right Cessation
1992
- 1992-11-27 FI FI925413A patent/FI106726B/fi active
1993
- 1993-12-29 LV LVP-93-1390A patent/LV10307B/en unknown
1994
- 1994-01-28 LT LTIP1820A patent/LT4000B/lt not_active IP Right Cessation
2000
- 2000-05-15 FI FI20001152A patent/FI119028B/fi not_active IP Right Cessation
2004
- 2004-08-20 DK DK200401262A patent/DK175852B1/da not_active IP Right Cessation

Also Published As

Publication number	Publication date
FI106726B (fi)	2001-03-30
DE3856331T2 (de)	1999-10-14
FI119028B (fi)	2008-06-30
US5217878A (en)	1993-06-08
DK200401262A (da)	2004-08-20
JP3026567B2 (ja)	2000-03-27
DK593988A (da)	1988-10-26
FI105483B (fi)	2000-08-31
FI20001152L (fi)	2000-05-15
NO884423L (no)	1988-10-05
PT86864B (pt)	1992-05-29
RU2023723C1 (ru)	1994-11-30
KR0127560B1 (ko)	1997-12-29
LTIP1820A (en)	1995-08-25
FI925413A0 (fi)	1992-11-27
HU213220B (en)	1997-03-28
DE3856339T2 (de)	1999-11-04
ATE181110T1 (de)	1999-06-15
LV10307A (lv)	1994-10-20
FI925413L (fi)	1992-11-27
NO884423D0 (no)	1988-10-05
ATE180016T1 (de)	1999-05-15
DK593988D0 (da)	1988-10-26
FI884904A0 (fi)	1988-10-24
LT4000B (en)	1996-06-25
DE3856331D1 (de)	1999-06-17
JPH01502876A (ja)	1989-10-05
AU1398088A (en)	1988-09-26
WO1988006624A3 (fr)	1988-11-17
DK175738B1 (da)	2005-02-07
DK175852B1 (da)	2005-04-04
KR890700665A (ko)	1989-04-26
CA1339100C (fr)	1997-07-29
FI884904L (fi)	1988-10-24
AU620026B2 (en)	1992-02-13
DE3856339D1 (de)	1999-07-15
WO1988006624A2 (fr)	1988-09-07
BG49049A3 (bg)	1991-07-15
BR8805647A (pt)	1989-10-31
CN88101681A (zh)	1988-11-09
PT86864A (pt)	1988-03-01

Publication	Publication Date	Title
US5217878A (en)	1993-06-08	Molecular cloning and expression of genes encoding proteolytic enzymes
EP0479396B1 (fr)	1999-06-09	Transformation de souches de Bacillus alkalophiles
AU620326B2 (en)	1992-02-20	Stable gene amplification in chromosomal dna of prokaryotic microorganisms
US4801537A (en)	1989-01-31	Vector for expression of polypeptides in bacilli
US5017477A (en)	1991-05-21	Enhancing DNA sequencies derived from the sacQ gene
US6100063A (en)	2000-08-08	Procaryotic cell comprising at least two copies of a gene
EP0149241A2 (fr)	1985-07-24	Séquence de bases d'ADN contenant des régions impliquées dans la production et la sécrétion d'une protéine, ADN recombinant comprenant la totalité ou une partie de la séquence de base d'ADN et méthode pour la production de protéines utilisant cet ADN recombinant
Honjo et al.	1984	Cloning and expression of the gene for neutral protease of Bacillus amyloliquefaciens in Bacillus subtilis
JP2501779B2 (ja)	1996-05-29	アルカリプロテア―ゼの製造方法
EP0303668A1 (fr)	1989-02-22	Vecteurs d'expression stables