[go: up one dir, main page]

LT3119886T - Rnr analizės būdas, išsaugantis kopijų skaičių - Google Patents

Rnr analizės būdas, išsaugantis kopijų skaičių

Info

Publication number
LT3119886T
LT3119886T LTEP15711509.8T LT15711509T LT3119886T LT 3119886 T LT3119886 T LT 3119886T LT 15711509 T LT15711509 T LT 15711509T LT 3119886 T LT3119886 T LT 3119886T
Authority
LT
Lithuania
Prior art keywords
analysis method
copy number
rna analysis
preserving rna
number preserving
Prior art date
Application number
LTEP15711509.8T
Other languages
English (en)
Inventor
Pamela Moll
Original Assignee
Lexogen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lexogen Gmbh filed Critical Lexogen Gmbh
Publication of LT3119886T publication Critical patent/LT3119886T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00Methods specially adapted for identifying library members
    • C40B20/04Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327RNAse, e.g. RNAseH
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/155Modifications characterised by incorporating/generating a new priming site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/173Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/179Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16Assays for determining copy number or wherein the copy number is of special importance

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTEP15711509.8T 2014-03-21 2015-03-20 Rnr analizės būdas, išsaugantis kopijų skaičių LT3119886T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14161135.0A EP2921556A1 (en) 2014-03-21 2014-03-21 Copy number preserving RNA analysis method
PCT/EP2015/055961 WO2015140307A1 (en) 2014-03-21 2015-03-20 Copy number preserving rna analysis method

Publications (1)

Publication Number Publication Date
LT3119886T true LT3119886T (lt) 2017-11-10

Family

ID=50342241

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEP15711509.8T LT3119886T (lt) 2014-03-21 2015-03-20 Rnr analizės būdas, išsaugantis kopijų skaičių

Country Status (13)

Country Link
US (1) US10597699B2 (lt)
EP (2) EP2921556A1 (lt)
JP (1) JP6608384B2 (lt)
KR (1) KR102398479B1 (lt)
CN (2) CN106414737A (lt)
AU (1) AU2015233383B2 (lt)
CA (1) CA2939056A1 (lt)
DK (1) DK3119886T3 (lt)
ES (1) ES2645423T3 (lt)
LT (1) LT3119886T (lt)
NO (1) NO3119886T3 (lt)
PL (1) PL3119886T3 (lt)
WO (1) WO2015140307A1 (lt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN110520540B (zh) 2017-04-13 2023-10-03 Imba-莫利库尔生物技术研究所 核酸修饰和鉴定方法
EP3388530A1 (en) 2017-04-13 2018-10-17 IMBA-Institut für Molekulare Biotechnologie GmbH Nucleic acid modification and identification method
WO2019060857A1 (en) * 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
CA3084439A1 (en) * 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
KR102767028B1 (ko) * 2018-06-06 2025-02-13 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트
EP3666904A1 (en) * 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method
CN110669830B (zh) * 2019-10-24 2023-05-23 裕策医疗器械江苏有限公司 一种低质量ffpe dna的处理方法、装置和存储介质
EP4023770A1 (en) 2021-01-05 2022-07-06 Narodowy Instytut Onkologii im. Marii Sklodowskiej-Curie Panstwowy Instytut Oddzial w Gliwicach A method of examining genes for the diagnosis of thyroid tumors, a set for the diagnosis of thyroid tumors and application
CN114854827A (zh) * 2022-05-18 2022-08-05 苏州极客基因科技有限公司 一种用于富集mRNA的磁珠复合物及其应用
EP4303324A1 (en) 2022-07-05 2024-01-10 Narodowy Instytut Onkologii im. Marii Sklodowskiej-Curie Panstwowy Instytut Oddzial w Gliwicach A method of distinguishing between benign and malignant thyroid nodules

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554730A (en) 1993-03-09 1996-09-10 Middlesex Sciences, Inc. Method and kit for making a polysaccharide-protein conjugate
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
ATE318327T1 (de) 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6406891B1 (en) * 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6544741B1 (en) 2000-07-12 2003-04-08 Quark Biotech, Inc. Sequence specific and sequence non-specific methods and materials for cDNA normalization and subtraction
WO2002059357A2 (en) 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP1371726A1 (en) * 2002-06-14 2003-12-17 Rijksuniversiteit Groningen Method for amplifying RNA
US7205128B2 (en) * 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
AT502823B1 (de) 2005-11-29 2007-06-15 Seitz Alexander Dr Polynukleotid-amplifikation
US7770107B2 (en) 2006-03-01 2010-08-03 Oracle International Corporation Methods and systems for extracting and processing translatable and transformable data from XSL files
DK3064599T3 (en) 2008-02-15 2019-04-08 Synthetic Genomics Inc METHODS IN VITRO FOR COMBINING AND COMBINATORY CONSTRUCTION OF NUCLEIC ACID MOLECULES
US7846666B2 (en) * 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
CN102203289A (zh) 2008-10-23 2011-09-28 恰根有限公司 利用内部校正定量检测rna
US20120058520A1 (en) * 2009-05-14 2012-03-08 Wako Pure Chemical Industries, Ltd. Method for synthesis of double-stranded dna corresponding to rna, and method for amplification of the dna
KR102087062B1 (ko) 2011-09-16 2020-03-11 렉소겐 게엠베하 핵산 전사 방법
CN102534813B (zh) 2011-11-15 2013-09-04 杭州联川生物技术有限公司 构建中小片段rna测序文库的方法
CN102533752B (zh) 2012-02-28 2015-01-21 盛司潼 一种Oligo dT引物及构建cDNA文库的方法
CN102817085B (zh) 2012-04-26 2014-07-16 深圳北京大学香港科技大学医学中心 一种快速逆转录microRNA文库的方法

Also Published As

Publication number Publication date
JP2017507663A (ja) 2017-03-23
JP6608384B2 (ja) 2019-11-20
PL3119886T3 (pl) 2017-12-29
AU2015233383B2 (en) 2021-06-24
US20180171384A1 (en) 2018-06-21
EP2921556A1 (en) 2015-09-23
WO2015140307A1 (en) 2015-09-24
EP3119886B1 (en) 2017-08-02
NO3119886T3 (lt) 2017-12-30
KR102398479B1 (ko) 2022-05-16
CN106414737A (zh) 2017-02-15
ES2645423T3 (es) 2017-12-05
AU2015233383A1 (en) 2016-09-08
US10597699B2 (en) 2020-03-24
EP3119886A1 (en) 2017-01-25
CN118755718A (zh) 2024-10-11
DK3119886T3 (da) 2017-11-13
KR20160138168A (ko) 2016-12-02
CA2939056A1 (en) 2015-09-24

Similar Documents

Publication Publication Date Title
GB201403096D0 (en) Sample preparation method
GB201402318D0 (en) Method for materials analysis
GB201406151D0 (en) Method
GB201409558D0 (en) Method
GB201408119D0 (en) Method
GB201406155D0 (en) Method
HUE044429T2 (hu) Analizáló patron és eljárás annak használatára
PL3119886T3 (pl) Sposób analizy RNA zachowujący liczbę kopii
GB201412977D0 (en) Single nucleotide detection method
ZA201608367B (en) Method for methylation analysis
GB201421664D0 (en) Method
GB201403470D0 (en) Method
HUE053440T2 (hu) Embrió-mintavételezõ eljárás
GB201410022D0 (en) Method
LU92498B1 (en) Method for transport analysis
GB201410101D0 (en) Method
GB201411500D0 (en) Method
GB201409234D0 (en) Method
GB201409128D0 (en) Method
GB201412736D0 (en) Method
GB201409101D0 (en) Method
GB201408649D0 (en) Method
GB201407971D0 (en) Method
GB201509656D0 (en) Data analysis method
GB201412356D0 (en) Method